Sort by
Items per page

Send to

Choose Destination

Search results

Items: 1 to 50 of 213


pH-Rate profiles establish that polyketide synthase dehydratase domains utilize a single-base mechanism.

Xie X, Cane DE.

Org Biomol Chem. 2018 Dec 5;16(47):9165-9170. doi: 10.1039/c8ob02637h.


Structure-Function Analysis of the Extended Conformation of a Polyketide Synthase Module.

Li X, Sevillano N, La Greca F, Deis L, Liu YC, Deller MC, Mathews II, Matsui T, Cane DE, Craik CS, Khosla C.

J Am Chem Soc. 2018 May 30;140(21):6518-6521. doi: 10.1021/jacs.8b02100. Epub 2018 May 18.


Substitution of Aromatic Residues with Polar Residues in the Active Site Pocket of epi-Isozizaene Synthase Leads to the Generation of New Cyclic Sesquiterpenes.

Blank PN, Barrow GH, Chou WKW, Duan L, Cane DE, Christianson DW.

Biochemistry. 2017 Oct 31;56(43):5798-5811. doi: 10.1021/acs.biochem.7b00895. Epub 2017 Oct 17.


Stereospecific Formation of E- and Z-Disubstituted Double Bonds by Dehydratase Domains from Modules 1 and 2 of the Fostriecin Polyketide Synthase.

Shah DD, You YO, Cane DE.

J Am Chem Soc. 2017 Oct 11;139(40):14322-14330. doi: 10.1021/jacs.7b08896. Epub 2017 Sep 27.


Elucidation of the Cryptic Methyl Group Epimerase Activity of Dehydratase Domains from Modular Polyketide Synthases Using a Tandem Modules Epimerase Assay.

Xie X, Garg A, Khosla C, Cane DE.

J Am Chem Soc. 2017 Jul 19;139(28):9507-9510. doi: 10.1021/jacs.7b05502. Epub 2017 Jul 7.


Elucidation of the Stereospecificity of C-Methyltransferases from trans-AT Polyketide Synthases.

Xie X, Khosla C, Cane DE.

J Am Chem Soc. 2017 May 3;139(17):6102-6105. doi: 10.1021/jacs.7b02911. Epub 2017 Apr 25.


Exploring the Influence of Domain Architecture on the Catalytic Function of Diterpene Synthases.

Pemberton TA, Chen M, Harris GG, Chou WK, Duan L, Köksal M, Genshaft AS, Cane DE, Christianson DW.

Biochemistry. 2017 Apr 11;56(14):2010-2023. doi: 10.1021/acs.biochem.7b00137. Epub 2017 Mar 31.


Incubation of 2-methylisoborneol synthase with the intermediate analog 2-methylneryl diphosphate.

Chou WK, Gould CA, Cane DE.

J Antibiot (Tokyo). 2017 May;70(5):625-631. doi: 10.1038/ja.2017.24. Epub 2017 Mar 1.


Mechanism and Stereochemistry of Polyketide Chain Elongation and Methyl Group Epimerization in Polyether Biosynthesis.

Xie X, Garg A, Khosla C, Cane DE.

J Am Chem Soc. 2017 Mar 1;139(8):3283-3292. doi: 10.1021/jacs.7b00278. Epub 2017 Feb 14.


Substitution of a Single Amino Acid Reverses the Regiospecificity of the Baeyer-Villiger Monooxygenase PntE in the Biosynthesis of the Antibiotic Pentalenolactone.

Chen K, Wu S, Zhu L, Zhang C, Xiang W, Deng Z, Ikeda H, Cane DE, Zhu D.

Biochemistry. 2016 Dec 6;55(48):6696-6704. Epub 2016 Nov 23.


The T296V Mutant of Amorpha-4,11-diene Synthase Is Defective in Allylic Diphosphate Isomerization but Retains the Ability To Cyclize the Intermediate (3R)-Nerolidyl Diphosphate to Amorpha-4,11-diene.

Li Z, Gao R, Hao Q, Zhao H, Cheng L, He F, Liu L, Liu X, Chou WK, Zhu H, Cane DE.

Biochemistry. 2016 Dec 6;55(48):6599-6604. Epub 2016 Nov 11.


The Cytochrome P450-Catalyzed Oxidative Rearrangement in the Final Step of Pentalenolactone Biosynthesis: Substrate Structure Determines Mechanism.

Duan L, Jogl G, Cane DE.

J Am Chem Soc. 2016 Sep 28;138(38):12678-89. doi: 10.1021/jacs.6b08610. Epub 2016 Sep 16.


Nature as organic chemist.

Cane DE.

J Antibiot (Tokyo). 2016 Jul;69(7):473-85. doi: 10.1038/ja.2016.55. No abstract available.


Roles of Conserved Active Site Residues in the Ketosynthase Domain of an Assembly Line Polyketide Synthase.

Robbins T, Kapilivsky J, Cane DE, Khosla C.

Biochemistry. 2016 Aug 16;55(32):4476-84. doi: 10.1021/acs.biochem.6b00639. Epub 2016 Aug 3.


Structure and mechanism of assembly line polyketide synthases.

Robbins T, Liu YC, Cane DE, Khosla C.

Curr Opin Struct Biol. 2016 Dec;41:10-18. doi: 10.1016/ Epub 2016 Jun 5. Review.


Protein-Protein Interactions, Not Substrate Recognition, Dominate the Turnover of Chimeric Assembly Line Polyketide Synthases.

Klaus M, Ostrowski MP, Austerjost J, Robbins T, Lowry B, Cane DE, Khosla C.

J Biol Chem. 2016 Jul 29;291(31):16404-15. doi: 10.1074/jbc.M116.730531. Epub 2016 May 31.


Probing the Role of Active Site Water in the Sesquiterpene Cyclization Reaction Catalyzed by Aristolochene Synthase.

Chen M, Chou WK, Al-Lami N, Faraldos JA, Allemann RK, Cane DE, Christianson DW.

Biochemistry. 2016 May 24;55(20):2864-74. doi: 10.1021/acs.biochem.6b00343. Epub 2016 May 12.


Recognition of acyl carrier proteins by ketoreductases in assembly line polyketide synthases.

Ostrowski MP, Cane DE, Khosla C.

J Antibiot (Tokyo). 2016 Jul;69(7):507-10. doi: 10.1038/ja.2016.41. Epub 2016 Apr 27.


A Turnstile Mechanism for the Controlled Growth of Biosynthetic Intermediates on Assembly Line Polyketide Synthases.

Lowry B, Li X, Robbins T, Cane DE, Khosla C.

ACS Cent Sci. 2016 Jan 27;2(1):14-20. Epub 2016 Jan 6.


Epimerase and Reductase Activities of Polyketide Synthase Ketoreductase Domains Utilize the Same Conserved Tyrosine and Serine Residues.

Xie X, Garg A, Keatinge-Clay AT, Khosla C, Cane DE.

Biochemistry. 2016 Mar 1;55(8):1179-86. doi: 10.1021/acs.biochem.6b00024. Epub 2016 Feb 12.


Structure and Function of Fusicoccadiene Synthase, a Hexameric Bifunctional Diterpene Synthase.

Chen M, Chou WK, Toyomasu T, Cane DE, Christianson DW.

ACS Chem Biol. 2016 Apr 15;11(4):889-99. doi: 10.1021/acschembio.5b00960. Epub 2016 Jan 6.


Structural Studies of Geosmin Synthase, a Bifunctional Sesquiterpene Synthase with αα Domain Architecture That Catalyzes a Unique Cyclization-Fragmentation Reaction Sequence.

Harris GG, Lombardi PM, Pemberton TA, Matsui T, Weiss TM, Cole KE, Köksal M, Murphy FV 4th, Vedula LS, Chou WK, Cane DE, Christianson DW.

Biochemistry. 2015 Dec 8;54(48):7142-55. doi: 10.1021/acs.biochem.5b01143. Epub 2015 Nov 24.


Novel terpenes generated by heterologous expression of bacterial terpene synthase genes in an engineered Streptomyces host.

Yamada Y, Arima S, Nagamitsu T, Johmoto K, Uekusa H, Eguchi T, Shin-ya K, Cane DE, Ikeda H.

J Antibiot (Tokyo). 2015 Jun;68(6):385-94. doi: 10.1038/ja.2014.171. Epub 2015 Jan 21.


Terpene synthases are widely distributed in bacteria.

Yamada Y, Kuzuyama T, Komatsu M, Shin-Ya K, Omura S, Cane DE, Ikeda H.

Proc Natl Acad Sci U S A. 2015 Jan 20;112(3):857-62. doi: 10.1073/pnas.1422108112. Epub 2014 Dec 22.


Elucidation of the cryptic epimerase activity of redox-inactive ketoreductase domains from modular polyketide synthases by tandem equilibrium isotope exchange.

Garg A, Xie X, Keatinge-Clay A, Khosla C, Cane DE.

J Am Chem Soc. 2014 Jul 23;136(29):10190-3. doi: 10.1021/ja5056998. Epub 2014 Jul 10.


Comparative analysis of the substrate specificity of trans- versus cis-acyltransferases of assembly line polyketide synthases.

Dunn BJ, Watts KR, Robbins T, Cane DE, Khosla C.

Biochemistry. 2014 Jun 17;53(23):3796-806. doi: 10.1021/bi5004316. Epub 2014 Jun 9.


Assembly line polyketide synthases: mechanistic insights and unsolved problems.

Khosla C, Herschlag D, Cane DE, Walsh CT.

Biochemistry. 2014 May 13;53(18):2875-83. doi: 10.1021/bi500290t. Epub 2014 May 1. Review.


Generation of complexity in fungal terpene biosynthesis: discovery of a multifunctional cytochrome P450 in the fumagillin pathway.

Lin HC, Tsunematsu Y, Dhingra S, Xu W, Fukutomi M, Chooi YH, Cane DE, Calvo AM, Watanabe K, Tang Y.

J Am Chem Soc. 2014 Mar 19;136(11):4426-36. doi: 10.1021/ja500881e. Epub 2014 Mar 11.


Reprogramming the chemodiversity of terpenoid cyclization by remolding the active site contour of epi-isozizaene synthase.

Li R, Chou WK, Himmelberger JA, Litwin KM, Harris GG, Cane DE, Christianson DW.

Biochemistry. 2014 Feb 25;53(7):1155-68. doi: 10.1021/bi401643u. Epub 2014 Feb 11.


Structure and stereospecificity of the dehydratase domain from the terminal module of the rifamycin polyketide synthase.

Gay D, You YO, Keatinge-Clay A, Cane DE.

Biochemistry. 2013 Dec 10;52(49):8916-28. doi: 10.1021/bi400988t. Epub 2013 Nov 25.


Coupled methyl group epimerization and reduction by polyketide synthase ketoreductase domains. Ketoreductase-catalyzed equilibrium isotope exchange.

Garg A, Khosla C, Cane DE.

J Am Chem Soc. 2013 Nov 6;135(44):16324-7. doi: 10.1021/ja408944s. Epub 2013 Oct 29.


In vitro reconstitution and analysis of the 6-deoxyerythronolide B synthase.

Lowry B, Robbins T, Weng CH, O'Brien RV, Cane DE, Khosla C.

J Am Chem Soc. 2013 Nov 13;135(45):16809-12. doi: 10.1021/ja409048k. Epub 2013 Nov 1.


Mechanistic insights from the binding of substrate and carbocation intermediate analogues to aristolochene synthase.

Chen M, Al-lami N, Janvier M, D'Antonio EL, Faraldos JA, Cane DE, Allemann RK, Christianson DW.

Biochemistry. 2013 Aug 13;52(32):5441-53. doi: 10.1021/bi400691v. Epub 2013 Aug 1.


Unexpected reactivity of 2-fluorolinalyl diphosphate in the active site of crystalline 2-methylisoborneol synthase.

Köksal M, Chou WK, Cane DE, Christianson DW.

Biochemistry. 2013 Aug 6;52(31):5247-55. doi: 10.1021/bi400797c. Epub 2013 Jul 22.


Stereochemistry of reductions catalyzed by methyl-epimerizing ketoreductase domains of polyketide synthases.

You YO, Khosla C, Cane DE.

J Am Chem Soc. 2013 May 22;135(20):7406-9. doi: 10.1021/ja4014776. Epub 2013 May 13.


Engineered Streptomyces avermitilis host for heterologous expression of biosynthetic gene cluster for secondary metabolites.

Komatsu M, Komatsu K, Koiwai H, Yamada Y, Kozone I, Izumikawa M, Hashimoto J, Takagi M, Omura S, Shin-ya K, Cane DE, Ikeda H.

ACS Synth Biol. 2013 Jul 19;2(7):384-96. doi: 10.1021/sb3001003. Epub 2013 Jan 17.


Mechanism and specificity of an acyltransferase domain from a modular polyketide synthase.

Dunn BJ, Cane DE, Khosla C.

Biochemistry. 2013 Mar 19;52(11):1839-41. doi: 10.1021/bi400185v. Epub 2013 Mar 5.


Product-mediated regulation of pentalenolactone biosynthesis in Streptomyces species by the MarR/SlyA family activators PenR and PntR.

Zhu D, Wang Y, Zhang M, Ikeda H, Deng Z, Cane DE.

J Bacteriol. 2013 Mar;195(6):1255-66. doi: 10.1128/JB.02079-12. Epub 2013 Jan 11.


Diversity and analysis of bacterial terpene synthases.

Yamada Y, Cane DE, Ikeda H.

Methods Enzymol. 2012;515:123-62. doi: 10.1016/B978-0-12-394290-6.00007-0. Review.


Precursor directed biosynthesis of an orthogonally functional erythromycin analogue: selectivity in the ribosome macrolide binding pocket.

Harvey CJ, Puglisi JD, Pande VS, Cane DE, Khosla C.

J Am Chem Soc. 2012 Jul 25;134(29):12259-65. doi: 10.1021/ja304682q. Epub 2012 Jul 11.


Effect of isotopically sensitive branching on product distribution for pentalenene synthase: support for a mechanism predicted by quantum chemistry.

Zu L, Xu M, Lodewyk MW, Cane DE, Peters RJ, Tantillo DJ.

J Am Chem Soc. 2012 Jul 18;134(28):11369-71. doi: 10.1021/ja3043245. Epub 2012 Jul 9.


Role of a conserved arginine residue in linkers between the ketosynthase and acyltransferase domains of multimodular polyketide synthases.

Yuzawa S, Kapur S, Cane DE, Khosla C.

Biochemistry. 2012 May 8;51(18):3708-10. doi: 10.1021/bi300399u. Epub 2012 Apr 24.


Structure of 2-methylisoborneol synthase from Streptomyces coelicolor and implications for the cyclization of a noncanonical C-methylated monoterpenoid substrate.

Köksal M, Chou WK, Cane DE, Christianson DW.

Biochemistry. 2012 Apr 10;51(14):3011-20. doi: 10.1021/bi201827a. Epub 2012 Mar 28.


Structure of geranyl diphosphate C-methyltransferase from Streptomyces coelicolor and implications for the mechanism of isoprenoid modification.

Köksal M, Chou WK, Cane DE, Christianson DW.

Biochemistry. 2012 Apr 10;51(14):3003-10. doi: 10.1021/bi300109c. Epub 2012 Mar 28.


Biochemistry: favouring the unfavoured.

Cane DE.

Nature. 2012 Mar 14;483(7389):285-6. doi: 10.1038/483285a. No abstract available.


Reprogramming a module of the 6-deoxyerythronolide B synthase for iterative chain elongation.

Kapur S, Lowry B, Yuzawa S, Kenthirapalan S, Chen AY, Cane DE, Khosla C.

Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4110-5. doi: 10.1073/pnas.1118734109. Epub 2012 Feb 27.


Essential role of the donor acyl carrier protein in stereoselective chain translocation to a fully reducing module of the nanchangmycin polyketide synthase.

Guo X, Liu T, Deng Z, Cane DE.

Biochemistry. 2012 Jan 31;51(4):879-87. doi: 10.1021/bi201768v. Epub 2012 Jan 17.


Exploration and mining of the bacterial terpenome.

Cane DE, Ikeda H.

Acc Chem Res. 2012 Mar 20;45(3):463-72. doi: 10.1021/ar200198d. Epub 2011 Oct 31. Review.


Supplemental Content

Support Center