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Use of isotopically labeled substrates reveals kinetic differences between human and bacterial serine palmitoyltransferase.

Harrison PJ, Gable K, Somashekarappa N, Kelly V, Clarke DJ, Naismith JH, Dunn TM, Campopiano DJ.

J Lipid Res. 2019 May;60(5):953-962. doi: 10.1194/jlr.M089367. Epub 2019 Feb 21.


Hydrogen Peroxide-Based Fluorometric Assay for Real-Time Monitoring of SAM-Dependent Methyltransferases.

Akhtar MK, Vijay D, Umbreen S, McLean CJ, Cai Y, Campopiano DJ, Loake GJ.

Front Bioeng Biotechnol. 2018 Oct 18;6:146. doi: 10.3389/fbioe.2018.00146. eCollection 2018.


Non-invasive 19F NMR analysis of a protein-templated N-acylhydrazone dynamic combinatorial library.

Ekström AG, Wang JT, Bella J, Campopiano DJ.

Org Biomol Chem. 2018 Nov 7;16(43):8144-8149. doi: 10.1039/c8ob01918e.


Sphingolipid biosynthesis in man and microbes.

Harrison PJ, Dunn TM, Campopiano DJ.

Nat Prod Rep. 2018 Sep 19;35(9):921-954. doi: 10.1039/c8np00019k. Review.


The carbon chain-selective adenylation enzyme TamA: the missing link between fatty acid and pyrrole natural product biosynthesis.

Marchetti PM, Kelly V, Simpson JP, Ward M, Campopiano DJ.

Org Biomol Chem. 2018 Apr 18;16(15):2735-2740. doi: 10.1039/c8ob00441b.


Structural evidence for the covalent modification of FabH by 4,5-dichloro-1,2-dithiol-3-one (HR45).

Ekström AG, Kelly V, Marles-Wright J, Cockroft SL, Campopiano DJ.

Org Biomol Chem. 2017 Aug 2;15(30):6310-6313. doi: 10.1039/c7ob01396e.


Using the pimeloyl-CoA synthetase adenylation fold to synthesize fatty acid thioesters.

Wang M, Moynié L, Harrison PJ, Kelly V, Piper A, Naismith JH, Campopiano DJ.

Nat Chem Biol. 2017 Jun;13(6):660-667. doi: 10.1038/nchembio.2361. Epub 2017 Apr 17.


Characterization of homologous sphingosine-1-phosphate lyase isoforms in the bacterial pathogen Burkholderia pseudomallei.

McLean CJ, Marles-Wright J, Custodio R, Lowther J, Kennedy AJ, Pollock J, Clarke DJ, Brown AR, Campopiano DJ.

J Lipid Res. 2017 Jan;58(1):137-150. doi: 10.1194/jlr.M071258. Epub 2016 Oct 26.


IL-1β-Induced Protection of Keratinocytes against Staphylococcus aureus-Secreted Proteases Is Mediated by Human β-Defensin 2.

Wang B, McHugh BJ, Qureshi A, Campopiano DJ, Clarke DJ, Fitzgerald JR, Dorin JR, Weller R, Davidson DJ.

J Invest Dermatol. 2017 Jan;137(1):95-105. doi: 10.1016/j.jid.2016.08.025. Epub 2016 Oct 1.


Characterization of secreted sphingosine-1-phosphate lyases required for virulence and intracellular survival of Burkholderia pseudomallei.

Custódio R, McLean CJ, Scott AE, Lowther J, Kennedy A, Clarke DJ, Campopiano DJ, Sarkar-Tyson M, Brown AR.

Mol Microbiol. 2016 Dec;102(6):1004-1019. doi: 10.1111/mmi.13531. Epub 2016 Sep 26.


Insight into Coenzyme A cofactor binding and the mechanism of acyl-transfer in an acylating aldehyde dehydrogenase from Clostridium phytofermentans.

Tuck LR, Altenbach K, Ang TF, Crawshaw AD, Campopiano DJ, Clarke DJ, Marles-Wright J.

Sci Rep. 2016 Feb 22;6:22108. doi: 10.1038/srep22108.


Determination of Protein Thiol Reduction Potential by Isotope Labeling and Intact Mass Measurement.

Thurlow SE, Kilgour DP, Campopiano DJ, Mackay CL, Langridge-Smith PR, Clarke DJ, Campbell CJ.

Anal Chem. 2016 Mar 1;88(5):2727-33. doi: 10.1021/acs.analchem.5b04195. Epub 2016 Feb 16.


Molecular basis of Streptococcus mutans sortase A inhibition by the flavonoid natural product trans-chalcone.

Wallock-Richards DJ, Marles-Wright J, Clarke DJ, Maitra A, Dodds M, Hanley B, Campopiano DJ.

Chem Commun (Camb). 2015 Jul 4;51(52):10483-5. doi: 10.1039/c5cc01816a.


Continuous colorimetric assay that enables high-throughput screening of N-acetylamino acid racemases.

Sánchez-Carrón G, Fleming T, Holt-Tiffin KE, Campopiano DJ.

Anal Chem. 2015 Apr 7;87(7):3923-8. doi: 10.1021/ac5047328. Epub 2015 Mar 13.


Garlic revisited: antimicrobial activity of allicin-containing garlic extracts against Burkholderia cepacia complex.

Wallock-Richards D, Doherty CJ, Doherty L, Clarke DJ, Place M, Govan JR, Campopiano DJ.

PLoS One. 2014 Dec 1;9(12):e112726. doi: 10.1371/journal.pone.0112726. eCollection 2014.


ACP-AasS you like it.

Campopiano DJ.

Chem Biol. 2014 Oct 23;21(10):1257-1259. doi: 10.1016/j.chembiol.2014.10.005.


The pyridoxal 5'-phosphate (PLP)-dependent enzyme serine palmitoyltransferase (SPT): effects of the small subunits and insights from bacterial mimics of human hLCB2a HSAN1 mutations.

Beattie AE, Gupta SD, Frankova L, Kazlauskaite A, Harmon JM, Dunn TM, Campopiano DJ.

Biomed Res Int. 2013;2013:194371. doi: 10.1155/2013/194371. Epub 2013 Sep 23.


Triazole biotin: a tight-binding biotinidase-resistant conjugate.

Germeroth AI, Hanna JR, Karim R, Kundel F, Lowther J, Neate PG, Blackburn EA, Wear MA, Campopiano DJ, Hulme AN.

Org Biomol Chem. 2013 Nov 28;11(44):7700-4. doi: 10.1039/c3ob41837e. Epub 2013 Oct 10.


The chemical basis of serine palmitoyltransferase inhibition by myriocin.

Wadsworth JM, Clarke DJ, McMahon SA, Lowther JP, Beattie AE, Langridge-Smith PR, Broughton HB, Dunn TM, Naismith JH, Campopiano DJ.

J Am Chem Soc. 2013 Sep 25;135(38):14276-85. doi: 10.1021/ja4059876. Epub 2013 Sep 11.


Reconstitution of the pyridoxal 5'-phosphate (PLP) dependent enzyme serine palmitoyltransferase (SPT) with pyridoxal reveals a crucial role for the phosphate during catalysis.

Beattie AE, Clarke DJ, Wadsworth JM, Lowther J, Sin HL, Campopiano DJ.

Chem Commun (Camb). 2013 Aug 14;49(63):7058-60. doi: 10.1039/c3cc43001d.


Ribosomally synthesized and post-translationally modified peptide natural products: overview and recommendations for a universal nomenclature.

Arnison PG, Bibb MJ, Bierbaum G, Bowers AA, Bugni TS, Bulaj G, Camarero JA, Campopiano DJ, Challis GL, Clardy J, Cotter PD, Craik DJ, Dawson M, Dittmann E, Donadio S, Dorrestein PC, Entian KD, Fischbach MA, Garavelli JS, Göransson U, Gruber CW, Haft DH, Hemscheidt TK, Hertweck C, Hill C, Horswill AR, Jaspars M, Kelly WL, Klinman JP, Kuipers OP, Link AJ, Liu W, Marahiel MA, Mitchell DA, Moll GN, Moore BS, Müller R, Nair SK, Nes IF, Norris GE, Olivera BM, Onaka H, Patchett ML, Piel J, Reaney MJ, Rebuffat S, Ross RP, Sahl HG, Schmidt EW, Selsted ME, Severinov K, Shen B, Sivonen K, Smith L, Stein T, Süssmuth RD, Tagg JR, Tang GL, Truman AW, Vederas JC, Walsh CT, Walton JD, Wenzel SC, Willey JM, van der Donk WA.

Nat Prod Rep. 2013 Jan;30(1):108-60. doi: 10.1039/c2np20085f. Review.


Partial complementation of Sinorhizobium meliloti bacA mutant phenotypes by the Mycobacterium tuberculosis BacA protein.

Arnold MF, Haag AF, Capewell S, Boshoff HI, James EK, McDonald R, Mair I, Mitchell AM, Kerscher B, Mitchell TJ, Mergaert P, Barry CE 3rd, Scocchi M, Zanda M, Campopiano DJ, Ferguson GP.

J Bacteriol. 2013 Jan;195(2):389-98. doi: 10.1128/JB.01445-12. Epub 2012 Nov 16.


An improved racemase/acylase biotransformation for the preparation of enantiomerically pure amino acids.

Baxter S, Royer S, Grogan G, Brown F, Holt-Tiffin KE, Taylor IN, Fotheringham IG, Campopiano DJ.

J Am Chem Soc. 2012 Nov 28;134(47):19310-3. doi: 10.1021/ja305438y. Epub 2012 Nov 15.


Bivalent enzyme inhibitors discovered using dynamic covalent chemistry.

Clipson AJ, Bhat VT, McNae I, Caniard AM, Campopiano DJ, Greaney MF.

Chemistry. 2012 Aug 20;18(34):10562-70. doi: 10.1002/chem.201201507. Epub 2012 Jul 10.


Structural, mechanistic and regulatory studies of serine palmitoyltransferase.

Lowther J, Naismith JH, Dunn TM, Campopiano DJ.

Biochem Soc Trans. 2012 Jun 1;40(3):547-54. doi: 10.1042/BST20110769. Review.


Ferric ion (hydr)oxo clusters in the "Venus flytrap" cleft of FbpA: Mössbauer, calorimetric and mass spectrometric studies.

Mukherjee A, Bilton PR, Mackay L, Janoschka A, Zhu H, Rea D, Langridge-Smith PR, Campopiano DJ, Teschner T, Trautwein AX, Schünemann V, Sadler PJ.

J Biol Inorg Chem. 2012 Apr;17(4):573-88. doi: 10.1007/s00775-012-0878-z. Epub 2012 Feb 17.


Role of a conserved arginine residue during catalysis in serine palmitoyltransferase.

Lowther J, Charmier G, Raman MC, Ikushiro H, Hayashi H, Campopiano DJ.

FEBS Lett. 2011 Jun 23;585(12):1729-34. doi: 10.1016/j.febslet.2011.04.013. Epub 2011 Apr 18.


Interaction of human β-defensin 2 (HBD2) with glycosaminoglycans.

Seo ES, Blaum BS, Vargues T, De Cecco M, Deakin JA, Lyon M, Barran PE, Campopiano DJ, Uhrín D.

Biochemistry. 2010 Dec 14;49(49):10486-95. doi: 10.1021/bi1011749. Epub 2010 Nov 17.


The serine palmitoyltransferase from Sphingomonas wittichii RW1: An interesting link to an unusual acyl carrier protein.

Raman MC, Johnson KA, Clarke DJ, Naismith JH, Campopiano DJ.

Biopolymers. 2010 Sep;93(9):811-22. doi: 10.1002/bip.21482.


Antimicrobial activity of CHIR-090, an inhibitor of lipopolysaccharide biosynthesis, against the Burkholderia cepacia complex.

Bodewits K, Raetz CR, Govan JR, Campopiano DJ.

Antimicrob Agents Chemother. 2010 Aug;54(8):3531-3. doi: 10.1128/AAC.01600-09. Epub 2010 Jun 1. No abstract available.


Nucleophilic catalysis of acylhydrazone equilibration for protein-directed dynamic covalent chemistry.

Bhat VT, Caniard AM, Luksch T, Brenk R, Campopiano DJ, Greaney MF.

Nat Chem. 2010 Jun;2(6):490-7. doi: 10.1038/nchem.658. Epub 2010 May 16.


Inhibition of the PLP-dependent enzyme serine palmitoyltransferase by cycloserine: evidence for a novel decarboxylative mechanism of inactivation.

Lowther J, Yard BA, Johnson KA, Carter LG, Bhat VT, Raman MC, Clarke DJ, Ramakers B, McMahon SA, Naismith JH, Campopiano DJ.

Mol Biosyst. 2010 Sep;6(9):1682-93. doi: 10.1039/c003743e. Epub 2010 May 5. Erratum in: Mol Biosyst. 2011 Dec;7(12):3375.


Conformational preferences of linear beta-defensins are revealed by ion mobility-mass spectrometry.

De Cecco M, Seo ES, Clarke DJ, McCullough BJ, Taylor K, Macmillan D, Dorin JR, Campopiano DJ, Barran PE.

J Phys Chem B. 2010 Feb 18;114(6):2312-8. doi: 10.1021/jp9111662.


Subdivision of the bacterioferritin comigratory protein family of bacterial peroxiredoxins based on catalytic activity.

Clarke DJ, Ortega XP, Mackay CL, Valvano MA, Govan JR, Campopiano DJ, Langridge-Smith P, Brown AR.

Biochemistry. 2010 Feb 16;49(6):1319-30. doi: 10.1021/bi901703m.


Synthesis and application of a new cleavable linker for "click"-based affinity chromatography.

Landi F, Johansson CM, Campopiano DJ, Hulme AN.

Org Biomol Chem. 2010 Jan 7;8(1):56-9. doi: 10.1039/b916693a. Epub 2009 Oct 13.


Insights into how nucleotide-binding domains power ABC transport.

Newstead S, Fowler PW, Bilton P, Carpenter EP, Sadler PJ, Campopiano DJ, Sansom MS, Iwata S.

Structure. 2009 Sep 9;17(9):1213-22. doi: 10.1016/j.str.2009.07.009.


Dying and necrotic neutrophils are anti-inflammatory secondary to the release of alpha-defensins.

Miles K, Clarke DJ, Lu W, Sibinska Z, Beaumont PE, Davidson DJ, Barr TA, Campopiano DJ, Gray M.

J Immunol. 2009 Aug 1;183(3):2122-32. doi: 10.4049/jimmunol.0804187. Epub 2009 Jul 13.


Efficient production of human beta-defensin 2 (HBD2) in Escherichia coli.

Vargues T, Morrison GJ, Seo ES, Clarke DJ, Fielder HL, Bennani J, Pathania U, Kilanowski F, Dorin JR, Govan JR, Mackay CL, Uhrín D, Campopiano DJ.

Protein Pept Lett. 2009;16(6):668-76.


Structural and functional studies of the biotin protein ligase from Aquifex aeolicus reveal a critical role for a conserved residue in target specificity.

Tron CM, McNae IW, Nutley M, Clarke DJ, Cooper A, Walkinshaw MD, Baxter RL, Campopiano DJ.

J Mol Biol. 2009 Mar 20;387(1):129-46.


Contributions of two UDP-glucose dehydrogenases to viability and polymyxin B resistance of Burkholderia cenocepacia.

Loutet SA, Bartholdson SJ, Govan JR, Campopiano DJ, Valvano MA.

Microbiology. 2009 Jun;155(Pt 6):2029-39. doi: 10.1099/mic.0.027607-0. Epub 2009 Apr 21.


The external aldimine form of serine palmitoyltransferase: structural, kinetic, and spectroscopic analysis of the wild-type enzyme and HSAN1 mutant mimics.

Raman MC, Johnson KA, Yard BA, Lowther J, Carter LG, Naismith JH, Campopiano DJ.

J Biol Chem. 2009 Jun 19;284(25):17328-39. doi: 10.1074/jbc.M109.008680. Epub 2009 Apr 17.


Interrogating the molecular details of the peroxiredoxin activity of the Escherichia coli bacterioferritin comigratory protein using high-resolution mass spectrometry.

Clarke DJ, Mackay CL, Campopiano DJ, Langridge-Smith P, Brown AR.

Biochemistry. 2009 May 12;48(18):3904-14. doi: 10.1021/bi900189e.


Preparation of isotopically labelled recombinant beta-defensin for NMR studies.

Seo ES, Vargues T, Clarke DJ, Uhrín D, Campopiano DJ.

Protein Expr Purif. 2009 Jun;65(2):179-84. doi: 10.1016/j.pep.2008.11.007. Epub 2008 Dec 3.


Effective binding and sensing of lipopolysaccharide: combining complementary pattern recognition receptors.

Ganesh V, Bodewits K, Bartholdson SJ, Natale D, Campopiano DJ, Mareque-Rivas JC.

Angew Chem Int Ed Engl. 2009;48(2):356-60. doi: 10.1002/anie.200804168. No abstract available.


Nitrilotriacetic acid-derivatized quantum dots for simple purification and site-selective fluorescent labeling of active proteins in a single step.

Gupta M, Caniard A, Touceda-Varela A, Campopiano DJ, Mareque-Rivas JC.

Bioconjug Chem. 2008 Oct;19(10):1964-7. doi: 10.1021/bc800273j. Epub 2008 Sep 19.


Plant host and sugar alcohol induced exopolysaccharide biosynthesis in the Burkholderia cepacia complex.

Bartholdson SJ, Brown AR, Mewburn BR, Clarke DJ, Fry SC, Campopiano DJ, Govan JR.

Microbiology. 2008 Aug;154(Pt 8):2513-21. doi: 10.1099/mic.0.2008/019216-0.


Analysis and separation of residues important for the chemoattractant and antimicrobial activities of beta-defensin 3.

Taylor K, Clarke DJ, McCullough B, Chin W, Seo E, Yang D, Oppenheim J, Uhrin D, Govan JR, Campopiano DJ, MacMillan D, Barran P, Dorin JR.

J Biol Chem. 2008 Mar 14;283(11):6631-9. doi: 10.1074/jbc.M709238200. Epub 2008 Jan 7.


Maturation of McjA precursor peptide into active microcin MccJ25.

Clarke DJ, Campopiano DJ.

Org Biomol Chem. 2007 Aug 21;5(16):2564-6.


The structure of serine palmitoyltransferase; gateway to sphingolipid biosynthesis.

Yard BA, Carter LG, Johnson KA, Overton IM, Dorward M, Liu H, McMahon SA, Oke M, Puech D, Barton GJ, Naismith JH, Campopiano DJ.

J Mol Biol. 2007 Jul 27;370(5):870-86. Epub 2007 May 10.


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