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Items: 1 to 50 of 81


Structure of voltage-dependent anion channel-tethered bilayer lipid membranes determined using neutron reflectivity.

Hoogerheide DP, Noskov SY, Kuszak AJ, Buchanan SK, Rostovtseva TK, Nanda H.

Acta Crystallogr D Struct Biol. 2018 Dec 1;74(Pt 12):1219-1232. doi: 10.1107/S2059798318011749. Epub 2018 Nov 30.


Hitting with a BAM: Selective Killing by Lectin-Like Bacteriocins.

Ghequire MGK, Swings T, Michiels J, Buchanan SK, De Mot R.

MBio. 2018 Mar 20;9(2). pii: e02138-17. doi: 10.1128/mBio.02138-17.


The ColM Family, Polymorphic Toxins Breaching the Bacterial Cell Wall.

Ghequire MGK, Buchanan SK, De Mot R.

MBio. 2018 Feb 13;9(1). pii: e02267-17. doi: 10.1128/mBio.02267-17.


Structural and functional insights into the role of BamD and BamE within the β-barrel assembly machinery in Neisseria gonorrhoeae.

Sikora AE, Wierzbicki IH, Zielke RA, Ryner RF, Korotkov KV, Buchanan SK, Noinaj N.

J Biol Chem. 2018 Jan 26;293(4):1106-1119. doi: 10.1074/jbc.RA117.000437. Epub 2017 Dec 11.


Mechanically Ventilating the Severe Asthmatic.

Laher AE, Buchanan SK.

J Intensive Care Med. 2018 Sep;33(9):491-501. doi: 10.1177/0885066617740079. Epub 2017 Nov 5. Review.


Insertion of proteins and lipopolysaccharide into the bacterial outer membrane.

Botos I, Noinaj N, Buchanan SK.

Philos Trans R Soc Lond B Biol Sci. 2017 Aug 5;372(1726). pii: 20160224. doi: 10.1098/rstb.2016.0224. Review.


Two-Partner Secretion: Combining Efficiency and Simplicity in the Secretion of Large Proteins for Bacteria-Host and Bacteria-Bacteria Interactions.

Guérin J, Bigot S, Schneider R, Buchanan SK, Jacob-Dubuisson F.

Front Cell Infect Microbiol. 2017 May 9;7:148. doi: 10.3389/fcimb.2017.00148. eCollection 2017. Review.


A review of hemodynamic monitoring techniques, methods and devices for the emergency physician.

Laher AE, Watermeyer MJ, Buchanan SK, Dippenaar N, Simo NCT, Motara F, Moolla M.

Am J Emerg Med. 2017 Sep;35(9):1335-1347. doi: 10.1016/j.ajem.2017.03.036. Epub 2017 Mar 18. Review.


A Natural Chimeric Pseudomonas Bacteriocin with Novel Pore-Forming Activity Parasitizes the Ferrichrome Transporter.

Ghequire MG, Kemland L, Anoz-Carbonell E, Buchanan SK, De Mot R.

MBio. 2017 Feb 21;8(1). pii: e01961-16. doi: 10.1128/mBio.01961-16.


The β-barrel assembly machinery in motion.

Noinaj N, Gumbart JC, Buchanan SK.

Nat Rev Microbiol. 2017 Apr;15(4):197-204. doi: 10.1038/nrmicro.2016.191. Epub 2017 Feb 20. Review.


Structural snapshots of the β-barrel assembly machinery.

Bakelar J, Buchanan SK, Noinaj N.

FEBS J. 2017 Jun;284(12):1778-1786. doi: 10.1111/febs.13960. Epub 2016 Nov 29. Review.


Structure of the NPr:EINNtr Complex: Mechanism for Specificity in Paralogous Phosphotransferase Systems.

Strickland M, Stanley AM, Wang G, Botos I, Schwieters CD, Buchanan SK, Peterkofsky A, Tjandra N.

Structure. 2016 Dec 6;24(12):2127-2137. doi: 10.1016/j.str.2016.10.007. Epub 2016 Nov 10.


Structural insight into the role of the Ton complex in energy transduction.

Celia H, Noinaj N, Zakharov SD, Bordignon E, Botos I, Santamaria M, Barnard TJ, Cramer WA, Lloubes R, Buchanan SK.

Nature. 2016 Oct 6;538(7623):60-65. doi: 10.1038/nature19757. Epub 2016 Sep 21.


Structural Insights into Substrate Recognition and Catalysis in Outer Membrane Protein B (OmpB) by Protein-lysine Methyltransferases from Rickettsia.

Abeykoon AH, Noinaj N, Choi BE, Wise L, He Y, Chao CC, Wang G, Gucek M, Ching WM, Chock PB, Buchanan SK, Yang DC.

J Biol Chem. 2016 Sep 16;291(38):19962-74. doi: 10.1074/jbc.M116.723460. Epub 2016 Jul 29.


From Constructs to Crystals - Towards Structure Determination of β-barrel Outer Membrane Proteins.

Noinaj N, Mayclin S, Stanley AM, Jao CC, Buchanan SK.

J Vis Exp. 2016 Jul 4;(113). doi: 10.3791/53245.


Structural and Functional Characterization of the LPS Transporter LptDE from Gram-Negative Pathogens.

Botos I, Majdalani N, Mayclin SJ, McCarthy JG, Lundquist K, Wojtowicz D, Barnard TJ, Gumbart JC, Buchanan SK.

Structure. 2016 Jun 7;24(6):965-976. doi: 10.1016/j.str.2016.03.026. Epub 2016 May 5.


The structure of the β-barrel assembly machinery complex.

Bakelar J, Buchanan SK, Noinaj N.

Science. 2016 Jan 8;351(6269):180-6. doi: 10.1126/science.aad3460.


Summary and Future Directions.

Noinaj N, Buchanan SK.

Methods Mol Biol. 2015;1329:279-80. doi: 10.1007/978-1-4939-2871-2_22.


Methods to Characterize Folding and Function of BamA Cross-Link Mutants.

Kuszak AJ, Noinaj N, Buchanan SK.

Methods Mol Biol. 2015;1329:137-47. doi: 10.1007/978-1-4939-2871-2_10.


Heat Modifiability of Outer Membrane Proteins from Gram-Negative Bacteria.

Noinaj N, Kuszak AJ, Buchanan SK.

Methods Mol Biol. 2015;1329:51-6. doi: 10.1007/978-1-4939-2871-2_4.


Fitting the Pieces of the β-Barrel Assembly Machinery Complex.

O'Neil PK, Rollauer SE, Noinaj N, Buchanan SK.

Biochemistry. 2015 Oct 20;54(41):6303-11. doi: 10.1021/acs.biochem.5b00852. Epub 2015 Oct 6. Review.


Outer membrane protein biogenesis in Gram-negative bacteria.

Rollauer SE, Sooreshjani MA, Noinaj N, Buchanan SK.

Philos Trans R Soc Lond B Biol Sci. 2015 Oct 5;370(1679). pii: 20150023. doi: 10.1098/rstb.2015.0023. Review.


Beyond the Crystal Structure: Insight into the Function and Vaccine Potential of TbpA Expressed by Neisseria gonorrhoeae.

Cash DR, Noinaj N, Buchanan SK, Cornelissen CN.

Infect Immun. 2015 Nov;83(11):4438-49. doi: 10.1128/IAI.00762-15. Epub 2015 Sep 8.


Evidence of Distinct Channel Conformations and Substrate Binding Affinities for the Mitochondrial Outer Membrane Protein Translocase Pore Tom40.

Kuszak AJ, Jacobs D, Gurnev PA, Shiota T, Louis JM, Lithgow T, Bezrukov SM, Rostovtseva TK, Buchanan SK.

J Biol Chem. 2015 Oct 23;290(43):26204-17. doi: 10.1074/jbc.M115.642173. Epub 2015 Sep 2.


The β-barrel membrane protein insertase machinery from Gram-negative bacteria.

Noinaj N, Rollauer SE, Buchanan SK.

Curr Opin Struct Biol. 2015 Apr;31:35-42. doi: 10.1016/ Epub 2015 Mar 19. Review.


Reconstitution of a nanomachine driving the assembly of proteins into bacterial outer membranes.

Shen HH, Leyton DL, Shiota T, Belousoff MJ, Noinaj N, Lu J, Holt SA, Tan K, Selkrig J, Webb CT, Buchanan SK, Martin LL, Lithgow T.

Nat Commun. 2014 Oct 24;5:5078. doi: 10.1038/ncomms6078.


Lateral opening and exit pore formation are required for BamA function.

Noinaj N, Kuszak AJ, Balusek C, Gumbart JC, Buchanan SK.

Structure. 2014 Jul 8;22(7):1055-62. doi: 10.1016/j.str.2014.05.008. Epub 2014 Jun 26.


FhaC takes a bow to FHA in the two-partner do-si-do.

Noinaj N, Buchanan SK.

Mol Microbiol. 2014 Jun;92(6):1155-8. doi: 10.1111/mmi.12627. Epub 2014 May 19.


An engineered palette of metal ion quenchable fluorescent proteins.

Yu X, Strub MP, Barnard TJ, Noinaj N, Piszczek G, Buchanan SK, Taraska JW.

PLoS One. 2014 Apr 21;9(4):e95808. doi: 10.1371/journal.pone.0095808. eCollection 2014.


Structural insights into the transport of small molecules across membranes.

Noinaj N, Buchanan SK.

Curr Opin Struct Biol. 2014 Aug;27:8-15. doi: 10.1016/ Epub 2014 Mar 28. Review.


Structural insight into the biogenesis of β-barrel membrane proteins.

Noinaj N, Kuszak AJ, Gumbart JC, Lukacik P, Chang H, Easley NC, Lithgow T, Buchanan SK.

Nature. 2013 Sep 19;501(7467):385-90. doi: 10.1038/nature12521. Epub 2013 Sep 1.


Molecular insight into substrate recognition and catalysis of Baeyer-Villiger monooxygenase MtmOIV, the key frame-modifying enzyme in the biosynthesis of anticancer agent mithramycin.

Bosserman MA, Downey T, Noinaj N, Buchanan SK, Rohr J.

ACS Chem Biol. 2013 Nov 15;8(11):2466-77. doi: 10.1021/cb400399b. Epub 2013 Sep 13.


Integrated nonlinear optical imaging microscope for on-axis crystal detection and centering at a synchrotron beamline.

Madden JT, Toth SJ, Dettmar CM, Newman JA, Oglesbee RA, Hedderich HG, Everly RM, Becker M, Ronau JA, Buchanan SK, Cherezov V, Morrow ME, Xu S, Ferguson D, Makarov O, Das C, Fischetti R, Simpson GJ.

J Synchrotron Radiat. 2013 Jul;20(Pt 4):531-40. doi: 10.1107/S0909049513007942. Epub 2013 May 3.


Structural insight into the lactoferrin receptors from pathogenic Neisseria.

Noinaj N, Cornelissen CN, Buchanan SK.

J Struct Biol. 2013 Oct;184(1):83-92. doi: 10.1016/j.jsb.2013.02.009. Epub 2013 Feb 24.


Specific targeting and killing of Gram-negative pathogens with an engineered phage lytic enzyme.

Lukacik P, Barnard TJ, Hinnebusch BJ, Buchanan SK.

Virulence. 2013 Jan 1;4(1):90-1. doi: 10.4161/viru.22683. No abstract available.


Using a bacteriocin structure to engineer a phage lysin that targets Yersinia pestis.

Lukacik P, Barnard TJ, Buchanan SK.

Biochem Soc Trans. 2012 Dec 1;40(6):1503-6. doi: 10.1042/BST20120209.


The transferrin-iron import system from pathogenic Neisseria species.

Noinaj N, Buchanan SK, Cornelissen CN.

Mol Microbiol. 2012 Oct;86(2):246-57. doi: 10.1111/mmi.12002. Epub 2012 Sep 7. Review.


Dynamic association of BAM complex modules includes surface exposure of the lipoprotein BamC.

Webb CT, Selkrig J, Perry AJ, Noinaj N, Buchanan SK, Lithgow T.

J Mol Biol. 2012 Sep 28;422(4):545-55. doi: 10.1016/j.jmb.2012.05.035. Epub 2012 Jun 6.


Structural engineering of a phage lysin that targets gram-negative pathogens.

Lukacik P, Barnard TJ, Keller PW, Chaturvedi KS, Seddiki N, Fairman JW, Noinaj N, Kirby TL, Henderson JP, Steven AC, Hinnebusch BJ, Buchanan SK.

Proc Natl Acad Sci U S A. 2012 Jun 19;109(25):9857-62. doi: 10.1073/pnas.1203472109. Epub 2012 Jun 7.


Crystal structures of the outer membrane domain of intimin and invasin from enterohemorrhagic E. coli and enteropathogenic Y. pseudotuberculosis.

Fairman JW, Dautin N, Wojtowicz D, Liu W, Noinaj N, Barnard TJ, Udho E, Przytycka TM, Cherezov V, Buchanan SK.

Structure. 2012 Jul 3;20(7):1233-43. doi: 10.1016/j.str.2012.04.011. Epub 2012 May 31.


Structural basis for iron piracy by pathogenic Neisseria.

Noinaj N, Easley NC, Oke M, Mizuno N, Gumbart J, Boura E, Steere AN, Zak O, Aisen P, Tajkhorshid E, Evans RW, Gorringe AR, Mason AB, Steven AC, Buchanan SK.

Nature. 2012 Feb 12;483(7387):53-8. doi: 10.1038/nature10823.


Molecular basis for the activation of a catalytic asparagine residue in a self-cleaving bacterial autotransporter.

Barnard TJ, Gumbart J, Peterson JH, Noinaj N, Easley NC, Dautin N, Kuszak AJ, Tajkhorshid E, Bernstein HD, Buchanan SK.

J Mol Biol. 2012 Jan 6;415(1):128-42. doi: 10.1016/j.jmb.2011.10.049. Epub 2011 Nov 7.


Structural insights into the catalytic mechanism of Escherichia coli selenophosphate synthetase.

Noinaj N, Wattanasak R, Lee DY, Wally JL, Piszczek G, Chock PB, Stadtman TC, Buchanan SK.

J Bacteriol. 2012 Jan;194(2):499-508. doi: 10.1128/JB.06012-11. Epub 2011 Nov 11.


Structural insights into Ail-mediated adhesion in Yersinia pestis.

Yamashita S, Lukacik P, Barnard TJ, Noinaj N, Felek S, Tsang TM, Krukonis ES, Hinnebusch BJ, Buchanan SK.

Structure. 2011 Nov 9;19(11):1672-82. doi: 10.1016/j.str.2011.08.010.


Role of the Yersinia pestis Ail protein in preventing a protective polymorphonuclear leukocyte response during bubonic plague.

Hinnebusch BJ, Jarrett CO, Callison JA, Gardner D, Buchanan SK, Plano GV.

Infect Immun. 2011 Dec;79(12):4984-9. doi: 10.1128/IAI.05307-11. Epub 2011 Oct 3.


The structural biology of β-barrel membrane proteins: a summary of recent reports.

Fairman JW, Noinaj N, Buchanan SK.

Curr Opin Struct Biol. 2011 Aug;21(4):523-31. doi: 10.1016/ Epub 2011 Jun 28. Review.


The crystal structure and mechanism of an unusual oxidoreductase, GilR, involved in gilvocarcin V biosynthesis.

Noinaj N, Bosserman MA, Schickli MA, Piszczek G, Kharel MK, Pahari P, Buchanan SK, Rohr J.

J Biol Chem. 2011 Jul 1;286(26):23533-43. doi: 10.1074/jbc.M111.247833. Epub 2011 May 10.


The crystal structure of BamB suggests interactions with BamA and its role within the BAM complex.

Noinaj N, Fairman JW, Buchanan SK.

J Mol Biol. 2011 Mar 25;407(2):248-60. doi: 10.1016/j.jmb.2011.01.042. Epub 2011 Jan 26.


Ancestral and derived protein import pathways in the mitochondrion of Reclinomonas americana.

Tong J, Dolezal P, Selkrig J, Crawford S, Simpson AG, Noinaj N, Buchanan SK, Gabriel K, Lithgow T.

Mol Biol Evol. 2011 May;28(5):1581-91. doi: 10.1093/molbev/msq305. Epub 2010 Nov 15.


Solute and Ion Transport: Outer Membrane Pores and Receptors.

Yamashita S, Buchanan SK.

EcoSal Plus. 2010 Sep;4(1). doi: 10.1128/ecosalplus.3.3.1.


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