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Evaluation of the WASPLab™ Segregation Software to Automatically Analyze Urine Cultures using Routine Blood and MacConkey Agars.

Faron ML, Buchan BW, Relich RF, Clark J, Ledeboer NA.

J Clin Microbiol. 2020 Jan 15. pii: JCM.01683-19. doi: 10.1128/JCM.01683-19. [Epub ahead of print]


Association Between Environmental Factors and Toxigenic Clostridioides difficile Carriage at Hospital Admission.

Muñoz-Price LS, Hanson R, Singh S, Nattinger AB, Penlesky A, Buchan BW, Ledeboer NA, Beyer K, Namin S, Zhou Y, Pezzin LE.

JAMA Netw Open. 2020 Jan 3;3(1):e1919132. doi: 10.1001/jamanetworkopen.2019.19132.


How frequently should sink drains be disinfected?

Ramos-Castaneda JA, Faron ML, Hyke J, Bell-Key D, Buchan BW, Nanchal R, Pintar P, Graham MB, Huerta S, Munoz-Price LS.

Infect Control Hosp Epidemiol. 2020 Mar;41(3):358-360. doi: 10.1017/ice.2019.316. Epub 2020 Jan 10.


A Multicenter Study of the Revogene C. difficile System for Detection of the Toxin B Gene from Unformed Stool Specimens.

Mashock MJ, Faron ML, Carroll KC, Dang C, Lewis S, Salimnia H, Lephart P, Loo VG, Schmitt BH, Young S, Buchan BW, Ledeboer NA.

J Clin Microbiol. 2020 Jan 28;58(2). pii: e01510-19. doi: 10.1128/JCM.01510-19. Print 2020 Jan 28.


Evaluation of WASPLab Software To Automatically Read chromID CPS Elite Agar for Reporting of Urine Cultures.

Faron ML, Buchan BW, Samra H, Ledeboer NA.

J Clin Microbiol. 2019 Dec 23;58(1). pii: e00540-19. doi: 10.1128/JCM.00540-19. Print 2019 Dec 23.


Multicenter Study Demonstrates Standardization Requirements for Mold Identification by MALDI-TOF MS.

Lau AF, Walchak RC, Miller HB, Slechta ES, Kamboj K, Riebe K, Robertson AE, Gilbreath JJ, Mitchell KF, Wallace MA, Bryson AL, Balada-Llasat JM, Bulman A, Buchan BW, Burnham CD, Butler-Wu S, Desai U, Doern CD, Hanson KE, Henderson CM, Kostrzewa M, Ledeboer NA, Maier T, Pancholi P, Schuetz AN, Shi G, Wengenack NL, Zhang SX, Zelazny AM, Frank KM.

Front Microbiol. 2019 Sep 20;10:2098. doi: 10.3389/fmicb.2019.02098. eCollection 2019.


Evaluation of a Novel Multiplex High-Definition PCR Assay for Detection of Tick-Borne Pathogens in Whole-Blood Specimens.

Buchan BW, Jobe DA, Mashock M, Gerstbrein D, Faron ML, Ledeboer NA, Callister SM.

J Clin Microbiol. 2019 Oct 23;57(11). pii: e00513-19. doi: 10.1128/JCM.00513-19. Print 2019 Nov.


Patient-to-Patient Transmission of Klebsiella pneumoniae Carbapenemase Variants with Reduced Ceftazidime-Avibactam Susceptibility.

Munoz-Price LS, Reeme AE, Buchan BW, Mettus RT, Mustapha MM, Van Tyne D, Shields RK, Doi Y.

Antimicrob Agents Chemother. 2019 Sep 23;63(10). pii: e00955-19. doi: 10.1128/AAC.00955-19. Print 2019 Oct.


Use of a cohorting-unit and systematic surveillance cultures to control a Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae outbreak.

Reeme AE, Bowler SL, Buchan BW, Graham MB, Behrens E, Singh S, Hong JC, Arvan J, Hyke JW, Palen L, Savage S, Seliger H, Huerta S, Ledeboer NA, Kotay S, Mathers AJ, Cooper VS, Mustapha MM, Mettus RT, Doi Y, Munoz-Price LS.

Infect Control Hosp Epidemiol. 2019 Jul;40(7):767-773. doi: 10.1017/ice.2019.99. Epub 2019 May 14.


Effectiveness of a hydrogen peroxide foam against bleach for the disinfection of sink drains.

Buchan BW, Arvan JA, Graham MB, Tarima S, Faron ML, Nanchal R, Munoz-Price LS.

Infect Control Hosp Epidemiol. 2019 Jun;40(6):724-726. doi: 10.1017/ice.2019.72. Epub 2019 Apr 17. No abstract available.


Campylobacter culture fails to correctly detect Campylobacter in 30% of positive patient stool specimens compared to non-cultural methods.

Buss JE, Cresse M, Doyle S, Buchan BW, Craft DW, Young S.

Eur J Clin Microbiol Infect Dis. 2019 Jun;38(6):1087-1093. doi: 10.1007/s10096-019-03499-x. Epub 2019 Feb 19.


The relevance of sink proximity to toilets on the detection of Klebsiella pneumoniae carbapenemase inside sink drains.

Buchan BW, Graham MB, Lindmair-Snell J, Arvan J, Ledeboer NA, Nanchal R, Munoz-Price LS.

Am J Infect Control. 2019 Jan;47(1):98-100. doi: 10.1016/j.ajic.2018.06.021. Epub 2018 Aug 29.


Clinical Evaluation of the iCubate iC-GPC Assay for Detection of Gram-Positive Bacteria and Resistance Markers from Positive Blood Cultures.

Granato PA, Unz MM, Widen RH, Silbert S, Young S, Heflin KL, Conover MS, Buchan BW, Ledeboer NA.

J Clin Microbiol. 2018 Aug 27;56(9). pii: e00485-18. doi: 10.1128/JCM.00485-18. Print 2018 Sep.


Elizabethkingia anophelis: Clinical Experience of an Academic Health System in Southeastern Wisconsin.

Figueroa Castro CE, Johnson C, Williams M, VanDerSlik A, Graham MB, Letzer D, Ledeboer N, Buchan BW, Block T, Borlaug G, Munoz-Price LS.

Open Forum Infect Dis. 2017 Dec 29;4(4):ofx251. doi: 10.1093/ofid/ofx251. eCollection 2017 Fall.


Multicenter Evaluation of the Accelerate PhenoTest BC Kit for Rapid Identification and Phenotypic Antimicrobial Susceptibility Testing Using Morphokinetic Cellular Analysis.

Pancholi P, Carroll KC, Buchan BW, Chan RC, Dhiman N, Ford B, Granato PA, Harrington AT, Hernandez DR, Humphries RM, Jindra MR, Ledeboer NA, Miller SA, Mochon AB, Morgan MA, Patel R, Schreckenberger PC, Stamper PD, Simner PJ, Tucci NE, Zimmerman C, Wolk DM.

J Clin Microbiol. 2018 Mar 26;56(4). pii: e01329-17. doi: 10.1128/JCM.01329-17. Print 2018 Apr.


Effective utilization of C. difficile PCR and identification of clinicopathologic factors associated with conversion to a positive result in symptomatic patients.

Mostafa ME, Flynn T, Hartley CP, Ledeboer NA, Buchan BW.

Diagn Microbiol Infect Dis. 2018 Apr;90(4):307-310. doi: 10.1016/j.diagmicrobio.2017.11.021. Epub 2017 Dec 6.


Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Use with Positive Blood Cultures: Methodology, Performance, and Optimization.

Faron ML, Buchan BW, Ledeboer NA.

J Clin Microbiol. 2017 Dec;55(12):3328-3338. doi: 10.1128/JCM.00868-17. Epub 2017 Aug 30. Review.


Evaluation of Copan FecalSwab as Specimen Type for Use in Xpert C. difficile Assay.

Mashock MJ, Faron ML, Buchan BW, Ledeboer NA.

J Clin Microbiol. 2017 Oct;55(10):3123-3129. doi: 10.1128/JCM.00369-17. Epub 2017 Aug 9.


Multicenter Evaluation of the Bruker MALDI Biotyper CA System for the Identification of Clinically Important Bacteria and Yeasts.

Wilson DA, Young S, Timm K, Novak-Weekley S, Marlowe EM, Madisen N, Lillie JL, Ledeboer NA, Smith R, Hyke J, Griego-Fullbright C, Jim P, Granato PA, Faron ML, Cumpio J, Buchan BW, Procop GW.

Am J Clin Pathol. 2017 Jun 1;147(6):623-631. doi: 10.1093/ajcp/aqw225.


Multicenter Evaluation of the Portrait Staph ID/R Blood Culture Panel for Rapid Identification of Staphylococci and Detection of the mecA Gene.

Denys GA, Collazo-Velez V, Young S, Daly JA, Couturier MR, Faron ML, Buchan BW, Ledeboer N.

J Clin Microbiol. 2017 Apr;55(4):1140-1146. doi: 10.1128/JCM.02348-16. Epub 2017 Jan 25.


Clinical Evaluation and Cost Analysis of Great Basin Shiga Toxin Direct Molecular Assay for Detection of Shiga Toxin-Producing Escherichia coli in Diarrheal Stool Specimens.

Faron ML, Ledeboer NA, Connolly J, Granato PA, Alkins BR, Dien Bard J, Daly JA, Young S, Buchan BW.

J Clin Microbiol. 2017 Feb;55(2):519-525. doi: 10.1128/JCM.01939-16. Epub 2016 Dec 7.


Automatic Digital Analysis of Chromogenic Media for Vancomycin-Resistant-Enterococcus Screens Using Copan WASPLab.

Faron ML, Buchan BW, Coon C, Liebregts T, van Bree A, Jansz AR, Soucy G, Korver J, Ledeboer NA.

J Clin Microbiol. 2016 Oct;54(10):2464-9. doi: 10.1128/JCM.01040-16. Epub 2016 Jul 13.


Treatment of Paecilomyces variotii pneumonia with posaconazole: case report and literature review.

Feldman R, Cockerham L, Buchan BW, Lu Z, Huang AM.

Mycoses. 2016 Dec;59(12):746-750. doi: 10.1111/myc.12525. Epub 2016 Jul 12. Review.


Multicenter Evaluation of the Solana Group A Streptococcus Assay: Comparison with Culture.

Uphoff TS, Buchan BW, Ledeboer NA, Granato PA, Daly JA, Marti TN.

J Clin Microbiol. 2016 Sep;54(9):2388-90. doi: 10.1128/JCM.01268-16. Epub 2016 Jun 29.


Multicenter Evaluation of Meridian Bioscience HSV 1&2 Molecular Assay for Detection of Herpes Simplex Virus 1 and 2 from Clinical Cutaneous and Mucocutaneous Specimens.

Faron ML, Ledeboer NA, Patel A, Beqa SH, Yen-Lieberman B, Kohn D, Leber AL, Mayne D, Northern WI, Buchan BW.

J Clin Microbiol. 2016 Aug;54(8):2008-13. doi: 10.1128/JCM.00483-16. Epub 2016 May 18.


Resistance Mechanisms, Epidemiology, and Approaches to Screening for Vancomycin-Resistant Enterococcus in the Health Care Setting.

Faron ML, Ledeboer NA, Buchan BW.

J Clin Microbiol. 2016 Oct;54(10):2436-47. doi: 10.1128/JCM.00211-16. Epub 2016 May 4. Review.


Comparison of ESwab and Wound Fiber Swab Specimen Collection Devices for Use with Xpert SA Nasal Complete Assay.

Beck ET, Buchan BW, Reymann GC, Ledeboer NA.

J Clin Microbiol. 2016 Jul;54(7):1904-1906. doi: 10.1128/JCM.00449-16. Epub 2016 Apr 27.


Is Staphylococcal Screening and Suppression an Effective Interventional Strategy for Reduction of Surgical Site Infection?

Edmiston CE Jr, Ledeboer NA, Buchan BW, Spencer M, Seabrook GR, Leaper D.

Surg Infect (Larchmt). 2016 Apr;17(2):158-66. doi: 10.1089/sur.2015.257. Epub 2016 Feb 2. Review.


Automated Scoring of Chromogenic Media for Detection of Methicillin-Resistant Staphylococcus aureus by Use of WASPLab Image Analysis Software.

Faron ML, Buchan BW, Vismara C, Lacchini C, Bielli A, Gesu G, Liebregts T, van Bree A, Jansz A, Soucy G, Korver J, Ledeboer NA.

J Clin Microbiol. 2016 Mar;54(3):620-4. doi: 10.1128/JCM.02778-15. Epub 2015 Dec 30.


Multicenter Evaluation of the Xpert Norovirus Assay for Detection of Norovirus Genogroups I and II in Fecal Specimens.

Gonzalez MD, Langley LC, Buchan BW, Faron ML, Maier M, Templeton K, Walker K, Popowitch EB, Miller MB, Rao A, Liebert UG, Ledeboer NA, Vinjé J, Burnham CA.

J Clin Microbiol. 2016 Jan;54(1):142-7. doi: 10.1128/JCM.02361-15. Epub 2015 Nov 11.


Multicenter Evaluation of the Bruker MALDI Biotyper CA System for the Identification of Clinical Aerobic Gram-Negative Bacterial Isolates.

Faron ML, Buchan BW, Hyke J, Madisen N, Lillie JL, Granato PA, Wilson DA, Procop GW, Novak-Weekley S, Marlowe E, Cumpio J, Griego-Fullbright C, Kindig S, Timm K, Young S, Ledeboer NA.

PLoS One. 2015 Nov 3;10(11):e0141350. doi: 10.1371/journal.pone.0141350. eCollection 2015.


Preliminary Evaluation of the Research-Use-Only (RUO) iCubate iC-GPC Assay for Identification of Select Gram-Positive Bacteria and Their Resistance Determinants in Blood Culture Broths.

Buchan BW, Reymann GC, Granato PA, Alkins BR, Jim P, Young S.

J Clin Microbiol. 2015 Dec;53(12):3931-4. doi: 10.1128/JCM.01934-15. Epub 2015 Oct 14.


Comparative Evaluation of AmpliVue HSV 1+2 Assay with ELVIS Culture for Detecting Herpes Simplex Virus 1 (HSV-1) and HSV-2 in Clinical Specimens.

Granato PA, Alkins BR, Yen-Lieberman B, Greene WH, Connolly J, Buchan BW, Ledeboer NA.

J Clin Microbiol. 2015 Dec;53(12):3922-5. doi: 10.1128/JCM.01905-15. Epub 2015 Oct 14.


Identification of Gram-Negative Bacteria and Genetic Resistance Determinants from Positive Blood Culture Broths by Use of the Verigene Gram-Negative Blood Culture Multiplex Microarray-Based Molecular Assay.

Ledeboer NA, Lopansri BK, Dhiman N, Cavagnolo R, Carroll KC, Granato P, Thomson R Jr, Butler-Wu SM, Berger H, Samuel L, Pancholi P, Swyers L, Hansen GT, Tran NK, Polage CR, Thomson KS, Hanson ND, Winegar R, Buchan BW.

J Clin Microbiol. 2015 Aug;53(8):2460-72. doi: 10.1128/JCM.00581-15. Epub 2015 May 20.


Detection of Group A Streptococcus in Pharyngeal Swab Specimens by Use of the AmpliVue GAS Isothermal Helicase-Dependent Amplification Assay.

Faron ML, Ledeboer NA, Granato P, Daly JA, Pierce K, Pancholi P, Uphoff TS, Buchan BW.

J Clin Microbiol. 2015 Jul;53(7):2365-7. doi: 10.1128/JCM.01085-15. Epub 2015 May 13.


Multicenter evaluation of the BD max enteric bacterial panel PCR assay for rapid detection of Salmonella spp., Shigella spp., Campylobacter spp. (C. jejuni and C. coli), and Shiga toxin 1 and 2 genes.

Harrington SM, Buchan BW, Doern C, Fader R, Ferraro MJ, Pillai DR, Rychert J, Doyle L, Lainesse A, Karchmer T, Mortensen JE.

J Clin Microbiol. 2015 May;53(5):1639-47. doi: 10.1128/JCM.03480-14. Epub 2015 Mar 4.


Comparison of the next-generation Xpert MRSA/SA BC assay and the GeneOhm StaphSR assay to routine culture for identification of Staphylococcus aureus and methicillin-resistant S. aureus in positive-blood-culture broths.

Buchan BW, Allen S, Burnham CA, McElvania TeKippe E, Davis T, Levi M, Mayne D, Pancholi P, Relich RF, Thomson R, Ledeboer NA.

J Clin Microbiol. 2015 Mar;53(3):804-9. doi: 10.1128/JCM.03108-14. Epub 2014 Dec 24.


Multicenter clinical evaluation of the Xpert GBS LB assay for detection of group B Streptococcus in prenatal screening specimens.

Buchan BW, Faron ML, Fuller D, Davis TE, Mayne D, Ledeboer NA.

J Clin Microbiol. 2015 Feb;53(2):443-8. doi: 10.1128/JCM.02598-14. Epub 2014 Nov 19.


Emerging technologies for the clinical microbiology laboratory.

Buchan BW, Ledeboer NA.

Clin Microbiol Rev. 2014 Oct;27(4):783-822. doi: 10.1128/CMR.00003-14. Review.


Multicenter evaluation of the Quidel Lyra Direct C. difficile nucleic acid amplification assay.

Beck ET, Buchan BW, Riebe KM, Alkins BR, Pancholi P, Granato PA, Ledeboer NA.

J Clin Microbiol. 2014 Jun;52(6):1998-2002. doi: 10.1128/JCM.03089-13. Epub 2014 Mar 26.


Clinical evaluation of the walk-away specimen processor and ESwab for recovery of Streptococcus agalactiae isolates in prenatal screening specimens.

Buchan BW, Olson WJ, Mackey TL, Ledeboer NA.

J Clin Microbiol. 2014 Jun;52(6):2166-8. doi: 10.1128/JCM.00374-14. Epub 2014 Mar 12.


Comparison of MALDI-TOF MS with HPLC and nucleic acid sequencing for the identification of Mycobacterium species in cultures using solid medium and broth.

Buchan BW, Riebe KM, Timke M, Kostrzewa M, Ledeboer NA.

Am J Clin Pathol. 2014 Jan;141(1):25-34. doi: 10.1309/AJCPBPUBUDEW2OAG.


Light microscopy, culture, molecular, and serologic methods for detection of herpes simplex virus.

Anderson NW, Buchan BW, Ledeboer NA.

J Clin Microbiol. 2014 Jan;52(1):2-8. doi: 10.1128/JCM.01966-13. Epub 2013 Oct 16. Review.


Multicenter evaluation of the Verigene Clostridium difficile nucleic acid assay.

Carroll KC, Buchan BW, Tan S, Stamper PD, Riebe KM, Pancholi P, Kelly C, Rao A, Fader R, Cavagnolo R, Watson W, Goering RV, Trevino EA, Weissfeld AS, Ledeboer NA.

J Clin Microbiol. 2013 Dec;51(12):4120-5. doi: 10.1128/JCM.01690-13. Epub 2013 Oct 2.


Clinical evaluation of a real-time PCR assay for identification of Salmonella, Shigella, Campylobacter (Campylobacter jejuni and C. coli), and shiga toxin-producing Escherichia coli isolates in stool specimens.

Buchan BW, Olson WJ, Pezewski M, Marcon MJ, Novicki T, Uphoff TS, Chandramohan L, Revell P, Ledeboer NA.

J Clin Microbiol. 2013 Dec;51(12):4001-7. doi: 10.1128/JCM.02056-13. Epub 2013 Sep 18.


Multiplex identification of gram-positive bacteria and resistance determinants directly from positive blood culture broths: evaluation of an automated microarray-based nucleic acid test.

Buchan BW, Ginocchio CC, Manii R, Cavagnolo R, Pancholi P, Swyers L, Thomson RB Jr, Anderson C, Kaul K, Ledeboer NA.

PLoS Med. 2013;10(7):e1001478. doi: 10.1371/journal.pmed.1001478. Epub 2013 Jul 2.


Multicenter clinical evaluation of VRESelect agar for identification of vancomycin-resistant Enterococcus faecalis and Enterococcus faecium.

Anderson NW, Buchan BW, Young CL, Newton DW, Brenke C, Lapsley L, Granato PA, Ledeboer NA.

J Clin Microbiol. 2013 Aug;51(8):2758-60. doi: 10.1128/JCM.00979-13. Epub 2013 Jun 12.


Multicenter clinical evaluation of the illumigene group A Streptococcus DNA amplification assay for detection of group A Streptococcus from pharyngeal swabs.

Anderson NW, Buchan BW, Mayne D, Mortensen JE, Mackey TL, Ledeboer NA.

J Clin Microbiol. 2013 May;51(5):1474-7. doi: 10.1128/JCM.00176-13. Epub 2013 Feb 27.


Advances in identification of clinical yeast isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry.

Buchan BW, Ledeboer NA.

J Clin Microbiol. 2013 May;51(5):1359-66. doi: 10.1128/JCM.03105-12. Epub 2013 Feb 20.

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