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Salmonella Translocated Effectors Recruit OSBP1 to the Phagosome to Promote Vacuolar Membrane Integrity.

Kolodziejek AM, Altura MA, Fan J, Petersen EM, Cook M, Brzovic PS, Miller SI.

Cell Rep. 2019 May 14;27(7):2147-2156.e5. doi: 10.1016/j.celrep.2019.04.021.


The Evolution of SlyA/RovA Transcription Factors from Repressors to Countersilencers in Enterobacteriaceae.

Will WR, Brzovic P, Le Trong I, Stenkamp RE, Lawrenz MB, Karlinsey JE, Navarre WW, Main-Hester K, Miller VL, Libby SJ, Fang FC.

mBio. 2019 Mar 5;10(2). pii: e00009-19. doi: 10.1128/mBio.00009-19.


The ubiquitin ligase SspH1 from Salmonella uses a modular and dynamic E3 domain to catalyze substrate ubiquitylation.

Cook M, Delbecq SP, Schweppe TP, Guttman M, Klevit RE, Brzovic PS.

J Biol Chem. 2019 Jan 18;294(3):783-793. doi: 10.1074/jbc.RA118.004247. Epub 2018 Nov 20.


A Bifunctional Role for the UHRF1 UBL Domain in the Control of Hemi-methylated DNA-Dependent Histone Ubiquitylation.

DaRosa PA, Harrison JS, Zelter A, Davis TN, Brzovic P, Kuhlman B, Klevit RE.

Mol Cell. 2018 Nov 15;72(4):753-765.e6. doi: 10.1016/j.molcel.2018.09.029. Epub 2018 Nov 1.


Mechanism of phosphoribosyl-ubiquitination mediated by a single Legionella effector.

Akturk A, Wasilko DJ, Wu X, Liu Y, Zhang Y, Qiu J, Luo ZQ, Reiter KH, Brzovic PS, Klevit RE, Mao Y.

Nature. 2018 May;557(7707):729-733. doi: 10.1038/s41586-018-0147-6. Epub 2018 May 23.


Tuning BRCA1 and BARD1 activity to investigate RING ubiquitin ligase mechanisms.

Stewart MD, Duncan ED, Coronado E, DaRosa PA, Pruneda JN, Brzovic PS, Klevit RE.

Protein Sci. 2017 Mar;26(3):475-483. doi: 10.1002/pro.3091. Epub 2017 Jan 23.


E2 enzymes: more than just middle men.

Stewart MD, Ritterhoff T, Klevit RE, Brzovic PS.

Cell Res. 2016 Apr;26(4):423-40. doi: 10.1038/cr.2016.35. Epub 2016 Mar 22. Review.


Structure and Energetics of Allosteric Regulation of HCN2 Ion Channels by Cyclic Nucleotides.

DeBerg HA, Brzovic PS, Flynn GE, Zagotta WN, Stoll S.

J Biol Chem. 2016 Jan 1;291(1):371-81. doi: 10.1074/jbc.M115.696450. Epub 2015 Nov 11.


Regulating the Regulators: Recent Revelations in the Control of E3 Ubiquitin Ligases.

Vittal V, Stewart MD, Brzovic PS, Klevit RE.

J Biol Chem. 2015 Aug 28;290(35):21244-51. doi: 10.1074/jbc.R115.675165. Epub 2015 Jul 17. Review.


Structural mechanism for the regulation of HCN ion channels by the accessory protein TRIP8b.

DeBerg HA, Bankston JR, Rosenbaum JC, Brzovic PS, Zagotta WN, Stoll S.

Structure. 2015 Apr 7;23(4):734-44. doi: 10.1016/j.str.2015.02.007. Epub 2015 Mar 19.


Intrinsic disorder drives N-terminal ubiquitination by Ube2w.

Vittal V, Shi L, Wenzel DM, Scaglione KM, Duncan ED, Basrur V, Elenitoba-Johnson KS, Baker D, Paulson HL, Brzovic PS, Klevit RE.

Nat Chem Biol. 2015 Jan;11(1):83-9. doi: 10.1038/nchembio.1700. Epub 2014 Dec 1.


The bacterial pathogen-ubiquitin interface: lessons learned from Shigella.

Tanner K, Brzovic P, Rohde JR.

Cell Microbiol. 2015 Jan;17(1):35-44. doi: 10.1111/cmi.12390. Epub 2014 Nov 28. Review.


E2~Ub conjugates regulate the kinase activity of Shigella effector OspG during pathogenesis.

Pruneda JN, Smith FD, Daurie A, Swaney DL, Villén J, Scott JD, Stadnyk AW, Le Trong I, Stenkamp RE, Klevit RE, Rohde JR, Brzovic PS.

EMBO J. 2014 Mar 3;33(5):437-49. doi: 10.1002/embj.201386386. Epub 2014 Jan 20.


Biochemical and structural characterization of the ubiquitin-conjugating enzyme UBE2W reveals the formation of a noncovalent homodimer.

Vittal V, Wenzel DM, Brzovic PS, Klevit RE.

Cell Biochem Biophys. 2013 Sep;67(1):103-10. doi: 10.1007/s12013-013-9633-5.


Activity-enhancing mutations in an E3 ubiquitin ligase identified by high-throughput mutagenesis.

Starita LM, Pruneda JN, Lo RS, Fowler DM, Kim HJ, Hiatt JB, Shendure J, Brzovic PS, Fields S, Klevit RE.

Proc Natl Acad Sci U S A. 2013 Apr 2;110(14):E1263-72. doi: 10.1073/pnas.1303309110. Epub 2013 Mar 18.


Structure of an E3:E2~Ub complex reveals an allosteric mechanism shared among RING/U-box ligases.

Pruneda JN, Littlefield PJ, Soss SE, Nordquist KA, Chazin WJ, Brzovic PS, Klevit RE.

Mol Cell. 2012 Sep 28;47(6):933-42. doi: 10.1016/j.molcel.2012.07.001. Epub 2012 Aug 9.


A Salmonella typhimurium-translocated glycerophospholipid:cholesterol acyltransferase promotes virulence by binding to the RhoA protein switch regions.

LaRock DL, Brzovic PS, Levin I, Blanc MP, Miller SI.

J Biol Chem. 2012 Aug 24;287(35):29654-63. doi: 10.1074/jbc.M112.363598. Epub 2012 Jun 27.


OTUB1 co-opts Lys48-linked ubiquitin recognition to suppress E2 enzyme function.

Juang YC, Landry MC, Sanches M, Vittal V, Leung CC, Ceccarelli DF, Mateo AR, Pruneda JN, Mao DY, Szilard RK, Orlicky S, Munro M, Brzovic PS, Klevit RE, Sicheri F, Durocher D.

Mol Cell. 2012 Feb 10;45(3):384-97. doi: 10.1016/j.molcel.2012.01.011. Erratum in: Mol Cell. 2012 May 25;46(4):549.


The acidic transcription activator Gcn4 binds the mediator subunit Gal11/Med15 using a simple protein interface forming a fuzzy complex.

Brzovic PS, Heikaus CC, Kisselev L, Vernon R, Herbig E, Pacheco D, Warfield L, Littlefield P, Baker D, Klevit RE, Hahn S.

Mol Cell. 2011 Dec 23;44(6):942-53. doi: 10.1016/j.molcel.2011.11.008.


UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids.

Wenzel DM, Lissounov A, Brzovic PS, Klevit RE.

Nature. 2011 Jun 2;474(7349):105-8. doi: 10.1038/nature09966. Epub 2011 May 1.


The essential Ubc4/Ubc5 function in yeast is HECT E3-dependent, and RING E3-dependent pathways require only monoubiquitin transfer by Ubc4.

Stoll KE, Brzovic PS, Davis TN, Klevit RE.

J Biol Chem. 2011 Apr 29;286(17):15165-70. doi: 10.1074/jbc.M110.203968. Epub 2011 Feb 25.


Ubiquitin in motion: structural studies of the ubiquitin-conjugating enzyme∼ubiquitin conjugate.

Pruneda JN, Stoll KE, Bolton LJ, Brzovic PS, Klevit RE.

Biochemistry. 2011 Mar 15;50(10):1624-33. doi: 10.1021/bi101913m. Epub 2011 Feb 21.


Identification of an unconventional E3 binding surface on the UbcH5 ~ Ub conjugate recognized by a pathogenic bacterial E3 ligase.

Levin I, Eakin C, Blanc MP, Klevit RE, Miller SI, Brzovic PS.

Proc Natl Acad Sci U S A. 2010 Feb 16;107(7):2848-53. doi: 10.1073/pnas.0914821107. Epub 2010 Feb 1.


Structural and functional characterization of the monomeric U-box domain from E4B.

Nordquist KA, Dimitrova YN, Brzovic PS, Ridenour WB, Munro KA, Soss SE, Caprioli RM, Klevit RE, Chazin WJ.

Biochemistry. 2010 Jan 19;49(2):347-55. doi: 10.1021/bi901620v.


Solution structure of the cGMP binding GAF domain from phosphodiesterase 5: insights into nucleotide specificity, dimerization, and cGMP-dependent conformational change.

Heikaus CC, Stout JR, Sekharan MR, Eakin CM, Rajagopal P, Brzovic PS, Beavo JA, Klevit RE.

J Biol Chem. 2008 Aug 15;283(33):22749-59. doi: 10.1074/jbc.M801577200. Epub 2008 Jun 4.


Crystal structure of the BARD1 ankyrin repeat domain and its functional consequences.

Fox D 3rd, Le Trong I, Rajagopal P, Brzovic PS, Stenkamp RE, Klevit RE.

J Biol Chem. 2008 Jul 25;283(30):21179-86. doi: 10.1074/jbc.M802333200. Epub 2008 May 14.


E2-BRCA1 RING interactions dictate synthesis of mono- or specific polyubiquitin chain linkages.

Christensen DE, Brzovic PS, Klevit RE.

Nat Struct Mol Biol. 2007 Oct;14(10):941-8. Epub 2007 Sep 16.


Allosteric regulation of tryptophan synthase channeling: the internal aldimine probed by trans-3-indole-3'-acrylate binding.

Casino P, Niks D, Ngo H, Pan P, Brzovic P, Blumenstein L, Barends TR, Schlichting I, Dunn MF.

Biochemistry. 2007 Jul 3;46(26):7728-39. Epub 2007 Jun 9.


Ubiquitin transfer from the E2 perspective: why is UbcH5 so promiscuous?

Brzovic PS, Klevit RE.

Cell Cycle. 2006 Dec;5(24):2867-73. Epub 2006 Dec 8.


A UbcH5/ubiquitin noncovalent complex is required for processive BRCA1-directed ubiquitination.

Brzovic PS, Lissounov A, Christensen DE, Hoyt DW, Klevit RE.

Mol Cell. 2006 Mar 17;21(6):873-80.


Binding and recognition in the assembly of an active BRCA1/BARD1 ubiquitin-ligase complex.

Brzovic PS, Keeffe JR, Nishikawa H, Miyamoto K, Fox D 3rd, Fukuda M, Ohta T, Klevit R.

Proc Natl Acad Sci U S A. 2003 May 13;100(10):5646-51. Epub 2003 May 5.


Structure of a BRCA1-BARD1 heterodimeric RING-RING complex.

Brzovic PS, Rajagopal P, Hoyt DW, King MC, Klevit RE.

Nat Struct Biol. 2001 Oct;8(10):833-7.


BRCA1 RING domain cancer-predisposing mutations. Structural consequences and effects on protein-protein interactions.

Brzovic PS, Meza JE, King MC, Klevit RE.

J Biol Chem. 2001 Nov 2;276(44):41399-406. Epub 2001 Aug 28.


Mapping the functional domains of BRCA1. Interaction of the ring finger domains of BRCA1 and BARD1.

Meza JE, Brzovic PS, King MC, Klevit RE.

J Biol Chem. 1999 Feb 26;274(9):5659-65.


Ca2+-dependent conformational changes in bovine GCAP-2.

Hughes RE, Brzovic PS, Dizhoor AM, Klevit RE, Hurley JB.

Protein Sci. 1998 Dec;7(12):2675-80.


Spectroscopic evidence for preexisting T- and R-state insulin hexamer conformations.

Choi WE, Borchardt D, Kaarsholm NC, Brzovic PS, Dunn MF.

Proteins. 1996 Dec;26(4):377-90.


Calcium-dependent solvation of the myristoyl group of recoverin.

Hughes RE, Brzovic PS, Klevit RE, Hurley JB.

Biochemistry. 1995 Sep 12;34(36):11410-6.


Ligand binding to wild-type and E-B13Q mutant insulins: a three-state allosteric model system showing half-site reactivity.

Bloom CR, Choi WE, Brzovic PS, Ha JJ, Huang ST, Kaarsholm NC, Dunn MF.

J Mol Biol. 1995 Jan 27;245(4):324-30.


Rapid-scanning ultraviolet/visible spectroscopy applied in stopped-flow studies.

Brzović PS, Dunn MF.

Methods Enzymol. 1995;246:168-201. No abstract available.


Structural asymmetry and half-site reactivity in the T to R allosteric transition of the insulin hexamer.

Brzović PS, Choi WE, Borchardt D, Kaarsholm NC, Dunn MF.

Biochemistry. 1994 Nov 8;33(44):13057-69.


Rapid-scanning stopped-flow spectrophotometry.

Brzović PS, Dunn MF.

Methods Biochem Anal. 1994;37:191-273. Review. No abstract available.


The tryptophan synthase bienzyme complex transfers indole between the alpha- and beta-sites via a 25-30 A long tunnel.

Dunn MF, Aguilar V, Brzović P, Drewe WF Jr, Houben KF, Leja CA, Roy M.

Biochemistry. 1990 Sep 18;29(37):8598-607.


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