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Items: 1 to 50 of 722

1.

The shark-tuna dichotomy: why tuna lay tiny eggs but sharks produce large offspring.

Sibly RM, Kodric-Brown A, Luna SM, Brown JH.

R Soc Open Sci. 2018 Aug 15;5(8):180453. doi: 10.1098/rsos.180453. eCollection 2018 Aug.

2.

Pillars Article: Three-Dimensional Structure of a Human Class II Histocompatibility Molecule Complexed with Superantigen. Nature. 1994. 368: 711-718.

Jardetzky TS, Brown JH, Gorga JC, Stern LJ, Urban RG, Chi YI, Stauffacher C, Strominger JL, Wiley DC.

J Immunol. 2018 Oct 1;201(7):1819-1826. No abstract available.

PMID:
30224361
3.

BioTIME: A database of biodiversity time series for the Anthropocene.

Dornelas M, Antão LH, Moyes F, Bates AE, Magurran AE, Adam D, Akhmetzhanova AA, Appeltans W, Arcos JM, Arnold H, Ayyappan N, Badihi G, Baird AH, Barbosa M, Barreto TE, Bässler C, Bellgrove A, Belmaker J, Benedetti-Cecchi L, Bett BJ, Bjorkman AD, Błażewicz M, Blowes SA, Bloch CP, Bonebrake TC, Boyd S, Bradford M, Brooks AJ, Brown JH, Bruelheide H, Budy P, Carvalho F, Castañeda-Moya E, Chen CA, Chamblee JF, Chase TJ, Siegwart Collier L, Collinge SK, Condit R, Cooper EJ, Cornelissen JHC, Cotano U, Kyle Crow S, Damasceno G, Davies CH, Davis RA, Day FP, Degraer S, Doherty TS, Dunn TE, Durigan G, Duffy JE, Edelist D, Edgar GJ, Elahi R, Elmendorf SC, Enemar A, Ernest SKM, Escribano R, Estiarte M, Evans BS, Fan TY, Turini Farah F, Loureiro Fernandes L, Farneda FZ, Fidelis A, Fitt R, Fosaa AM, Daher Correa Franco GA, Frank GE, Fraser WR, García H, Cazzolla Gatti R, Givan O, Gorgone-Barbosa E, Gould WA, Gries C, Grossman GD, Gutierréz JR, Hale S, Harmon ME, Harte J, Haskins G, Henshaw DL, Hermanutz L, Hidalgo P, Higuchi P, Hoey A, Van Hoey G, Hofgaard A, Holeck K, Hollister RD, Holmes R, Hoogenboom M, Hsieh CH, Hubbell SP, Huettmann F, Huffard CL, Hurlbert AH, Macedo Ivanauskas N, Janík D, Jandt U, Jażdżewska A, Johannessen T, Johnstone J, Jones J, Jones FAM, Kang J, Kartawijaya T, Keeley EC, Kelt DA, Kinnear R, Klanderud K, Knutsen H, Koenig CC, Kortz AR, Král K, Kuhnz LA, Kuo CY, Kushner DJ, Laguionie-Marchais C, Lancaster LT, Min Lee C, Lefcheck JS, Lévesque E, Lightfoot D, Lloret F, Lloyd JD, López-Baucells A, Louzao M, Madin JS, Magnússon B, Malamud S, Matthews I, McFarland KP, McGill B, McKnight D, McLarney WO, Meador J, Meserve PL, Metcalfe DJ, Meyer CFJ, Michelsen A, Milchakova N, Moens T, Moland E, Moore J, Mathias Moreira C, Müller J, Murphy G, Myers-Smith IH, Myster RW, Naumov A, Neat F, Nelson JA, Paul Nelson M, Newton SF, Norden N, Oliver JC, Olsen EM, Onipchenko VG, Pabis K, Pabst RJ, Paquette A, Pardede S, Paterson DM, Pélissier R, Peñuelas J, Pérez-Matus A, Pizarro O, Pomati F, Post E, Prins HHT, Priscu JC, Provoost P, Prudic KL, Pulliainen E, Ramesh BR, Mendivil Ramos O, Rassweiler A, Rebelo JE, Reed DC, Reich PB, Remillard SM, Richardson AJ, Richardson JP, van Rijn I, Rocha R, Rivera-Monroy VH, Rixen C, Robinson KP, Ribeiro Rodrigues R, de Cerqueira Rossa-Feres D, Rudstam L, Ruhl H, Ruz CS, Sampaio EM, Rybicki N, Rypel A, Sal S, Salgado B, Santos FAM, Savassi-Coutinho AP, Scanga S, Schmidt J, Schooley R, Setiawan F, Shao KT, Shaver GR, Sherman S, Sherry TW, Siciński J, Sievers C, da Silva AC, Rodrigues da Silva F, Silveira FL, Slingsby J, Smart T, Snell SJ, Soudzilovskaia NA, Souza GBG, Maluf Souza F, Castro Souza V, Stallings CD, Stanforth R, Stanley EH, Mauro Sterza J, Stevens M, Stuart-Smith R, Rondon Suarez Y, Supp S, Yoshio Tamashiro J, Tarigan S, Thiede GP, Thorn S, Tolvanen A, Teresa Zugliani Toniato M, Totland Ø, Twilley RR, Vaitkus G, Valdivia N, Vallejo MI, Valone TJ, Van Colen C, Vanaverbeke J, Venturoli F, Verheye HM, Vianna M, Vieira RP, Vrška T, Quang Vu C, Van Vu L, Waide RB, Waldock C, Watts D, Webb S, Wesołowski T, White EP, Widdicombe CE, Wilgers D, Williams R, Williams SB, Williamson M, Willig MR, Willis TJ, Wipf S, Woods KD, Woehler EJ, Zawada K, Zettler ML, Hickler T.

Glob Ecol Biogeogr. 2018 Jul;27(7):760-786. doi: 10.1111/geb.12729. Epub 2018 Jul 24.

4.

RhoA regulates Drp1 mediated mitochondrial fission through ROCK to protect cardiomyocytes.

Brand CS, Tan VP, Brown JH, Miyamoto S.

Cell Signal. 2018 Oct;50:48-57. doi: 10.1016/j.cellsig.2018.06.012. Epub 2018 Jun 25.

PMID:
29953931
5.

YAP and MRTF-A, transcriptional co-activators of RhoA-mediated gene expression, are critical for glioblastoma tumorigenicity.

Yu OM, Benitez JA, Plouffe SW, Ryback D, Klein A, Smith J, Greenbaum J, Delatte B, Rao A, Guan KL, Furnari FB, Chaim OM, Miyamoto S, Brown JH.

Oncogene. 2018 Jun 11. doi: 10.1038/s41388-018-0301-5. [Epub ahead of print]

PMID:
29887596
6.

A Tailorable In-Situ Light-Activated Biodegradable Vascular Scaffold.

Albaghdadi MS, Yang J, Brown JH, Mansukhani NA, Ameer GA, Kibbe MR.

Adv Mater Technol. 2017 Apr;2(4). pii: 1600243. doi: 10.1002/admt.201600243. Epub 2017 Feb 20.

7.

Nanofibrous PLGA electrospun scaffolds modified with type I collagen influence hepatocyte function and support viability in vitro.

Brown JH, Das P, DiVito MD, Ivancic D, Tan LP, Wertheim JA.

Acta Biomater. 2018 Jun;73:217-227. doi: 10.1016/j.actbio.2018.02.009. Epub 2018 Feb 15.

PMID:
29454157
8.

Chronic inhalation of e-cigarette vapor containing nicotine disrupts airway barrier function and induces systemic inflammation and multiorgan fibrosis in mice.

Crotty Alexander LE, Drummond CA, Hepokoski M, Mathew D, Moshensky A, Willeford A, Das S, Singh P, Yong Z, Lee JH, Vega K, Du A, Shin J, Javier C, Tian J, Brown JH, Breen EC.

Am J Physiol Regul Integr Comp Physiol. 2018 Jun 1;314(6):R834-R847. doi: 10.1152/ajpregu.00270.2017. Epub 2018 Jan 31.

PMID:
29384700
9.

Equal fitness paradigm explained by a trade-off between generation time and energy production rate.

Brown JH, Hall CAS, Sibly RM.

Nat Ecol Evol. 2018 Feb;2(2):262-268. doi: 10.1038/s41559-017-0430-1. Epub 2018 Jan 8.

PMID:
29311701
10.

In Situ Dark Adaptation Enhances the Efficiency of DNA Extraction from Mature Pin Oak (Quercus palustris) Leaves, Facilitating the Identification of Partial Sequences of the 18S rRNA and Isoprene Synthase (IspS) Genes.

Barta CE, Bolander B, Bilby SR, Brown JH, Brown RN, Duryee AM, Edelman DR, Gray CE, Gossett C, Haddock AG, Helsel MM, Jones AD, Klingseis ME, Leslie K, Miles EW, Prawitz RA.

Plants (Basel). 2017 Oct 24;6(4). pii: E52. doi: 10.3390/plants6040052.

11.

C-terminal phosphorylation of NaV1.5 impairs FGF13-dependent regulation of channel inactivation.

Burel S, Coyan FC, Lorenzini M, Meyer MR, Lichti CF, Brown JH, Loussouarn G, Charpentier F, Nerbonne JM, Townsend RR, Maier LS, Marionneau C.

J Biol Chem. 2017 Oct 20;292(42):17431-17448. doi: 10.1074/jbc.M117.787788. Epub 2017 Sep 7.

PMID:
28882890
12.

Correspondence: Reply to 'Analytical flaws in a continental-scale forest soil microbial diversity study'.

Zhou J, Deng Y, Shen L, Wen C, Yan Q, Ning D, Qin Y, Xue K, Wu L, He Z, Voordeckers JW, Van Nostrand JD, Buzzard V, Michaletz ST, Enquist BJ, Weiser MD, Kaspari M, Waide R, Yang Y, Brown JH.

Nat Commun. 2017 Jun 6;8:15583. doi: 10.1038/ncomms15583. No abstract available.

13.

Sphingosine 1-phosphate receptor 3 and RhoA signaling mediate inflammatory gene expression in astrocytes.

Dusaban SS, Chun J, Rosen H, Purcell NH, Brown JH.

J Neuroinflammation. 2017 Jun 2;14(1):111. doi: 10.1186/s12974-017-0882-x.

14.

Calcium/Calmodulin-Dependent Protein Kinase II Activity Persists During Chronic β-Adrenoceptor Blockade in Experimental and Human Heart Failure.

Dewenter M, Neef S, Vettel C, Lämmle S, Beushausen C, Zelarayan LC, Katz S, von der Lieth A, Meyer-Roxlau S, Weber S, Wieland T, Sossalla S, Backs J, Brown JH, Maier LS, El-Armouche A.

Circ Heart Fail. 2017 May;10(5):e003840. doi: 10.1161/CIRCHEARTFAILURE.117.003840.

15.

Who is and isn't having babies with Down syndrome in western Sydney: a ten year hospital cohort study.

Moses RM, Brown JH, Wright DC, Diplock H, Melov SJ, McGee TM.

Aust N Z J Obstet Gynaecol. 2017 Apr;57(2):146-151. doi: 10.1111/ajo.12617. Epub 2017 Mar 29.

PMID:
28369907
16.

CaMKIIδ subtypes differentially regulate infarct formation following ex vivo myocardial ischemia/reperfusion through NF-κB and TNF-α.

Gray CB, Suetomi T, Xiang S, Mishra S, Blackwood EA, Glembotski CC, Miyamoto S, Westenbrink BD, Brown JH.

J Mol Cell Cardiol. 2017 Feb;103:48-55. doi: 10.1016/j.yjmcc.2017.01.002. Epub 2017 Jan 7.

17.

Selective coupling of the S1P3 receptor subtype to S1P-mediated RhoA activation and cardioprotection.

Yung BS, Brand CS, Xiang SY, Gray CB, Means CK, Rosen H, Chun J, Purcell NH, Brown JH, Miyamoto S.

J Mol Cell Cardiol. 2017 Feb;103:1-10. doi: 10.1016/j.yjmcc.2016.12.008. Epub 2016 Dec 23.

18.

Temperature mediates continental-scale diversity of microbes in forest soils.

Zhou J, Deng Y, Shen L, Wen C, Yan Q, Ning D, Qin Y, Xue K, Wu L, He Z, Voordeckers JW, Nostrand JD, Buzzard V, Michaletz ST, Enquist BJ, Weiser MD, Kaspari M, Waide R, Yang Y, Brown JH.

Nat Commun. 2016 Jul 5;7:12083. doi: 10.1038/ncomms12083.

19.

The macroecology of infectious diseases: a new perspective on global-scale drivers of pathogen distributions and impacts.

Stephens PR, Altizer S, Smith KF, Alonso Aguirre A, Brown JH, Budischak SA, Byers JE, Dallas TA, Jonathan Davies T, Drake JM, Ezenwa VO, Farrell MJ, Gittleman JL, Han BA, Huang S, Hutchinson RA, Johnson P, Nunn CL, Onstad D, Park A, Vazquez-Prokopec GM, Schmidt JP, Poulin R.

Ecol Lett. 2016 Sep;19(9):1159-71. doi: 10.1111/ele.12644. Epub 2016 Jun 28. Review.

PMID:
27353433
20.

Exercise training reverses myocardial dysfunction induced by CaMKIIδC overexpression by restoring Ca2+ homeostasis.

Høydal MA, Stølen TO, Kettlewell S, Maier LS, Brown JH, Sowa T, Catalucci D, Condorelli G, Kemi OJ, Smith GL, Wisløff U.

J Appl Physiol (1985). 2016 Jul 1;121(1):212-20. doi: 10.1152/japplphysiol.00188.2016. Epub 2016 May 26.

21.

Sphingosine 1-phosphate elicits RhoA-dependent proliferation and MRTF-A mediated gene induction in CPCs.

Castaldi A, Chesini GP, Taylor AE, Sussman MA, Brown JH, Purcell NH.

Cell Signal. 2016 Aug;28(8):871-9. doi: 10.1016/j.cellsig.2016.04.006. Epub 2016 Apr 14.

22.

Biogeographic patterns of soil diazotrophic communities across six forests in the North America.

Tu Q, Deng Y, Yan Q, Shen L, Lin L, He Z, Wu L, Van Nostrand JD, Buzzard V, Michaletz ST, Enquist BJ, Weiser MD, Kaspari M, Waide RB, Brown JH, Zhou J.

Mol Ecol. 2016 Jun;25(12):2937-48. doi: 10.1111/mec.13651. Epub 2016 May 21. Erratum in: Mol Ecol. 2017 Apr;26(8):2405.

PMID:
27085668
23.

Long-term monitoring and experimental manipulation of a Chihuahuan desert ecosystem near Portal, Arizona (1977-2013).

Morgan Ernest SK, Yenni GM, Allington G, Christensen EM, Geluso K, Goheen JR, Schutzenhofer MR, Supp SR, Thibault KM, Brown JH, Valone TJ.

Ecology. 2016 Apr;97(4):1082. doi: 10.1890/15-2115.1.

PMID:
28792597
24.

Drp1 and Mitochondrial Autophagy Lend a Helping Hand in Adaptation to Pressure Overload.

Miyamoto S, Brown JH.

Circulation. 2016 Mar 29;133(13):1225-7. doi: 10.1161/CIRCULATIONAHA.116.021796. Epub 2016 Feb 25. No abstract available.

25.

Reductions in the Cardiac Transient Outward K+ Current Ito Caused by Chronic β-Adrenergic Receptor Stimulation Are Partly Rescued by Inhibition of Nuclear Factor κB.

Panama BK, Korogyi AS, Aschar-Sobbi R, Oh Y, Gray CB, Gang H, Brown JH, Kirshenbaum LA, Backx PH.

J Biol Chem. 2016 Feb 19;291(8):4156-65. doi: 10.1074/jbc.M115.694984. Epub 2016 Jan 7.

26.

Metabolic heat production and thermal conductance are mass-independent adaptations to thermal environment in birds and mammals.

Fristoe TS, Burger JR, Balk MA, Khaliq I, Hof C, Brown JH.

Proc Natl Acad Sci U S A. 2015 Dec 29;112(52):15934-9. doi: 10.1073/pnas.1521662112. Epub 2015 Dec 14.

27.

Fundamental insights into ontogenetic growth from theory and fish.

Sibly RM, Baker J, Grady JM, Luna SM, Kodric-Brown A, Venditti C, Brown JH.

Proc Natl Acad Sci U S A. 2015 Nov 10;112(45):13934-9. doi: 10.1073/pnas.1518823112. Epub 2015 Oct 27.

28.

Bitopic Sphingosine 1-Phosphate Receptor 3 (S1P3) Antagonist Rescue from Complete Heart Block: Pharmacological and Genetic Evidence for Direct S1P3 Regulation of Mouse Cardiac Conduction.

Sanna MG, Vincent KP, Repetto E, Nguyen N, Brown SJ, Abgaryan L, Riley SW, Leaf NB, Cahalan SM, Kiosses WB, Kohno Y, Brown JH, McCulloch AD, Rosen H, Gonzalez-Cabrera PJ.

Mol Pharmacol. 2016 Jan;89(1):176-86. doi: 10.1124/mol.115.100222. Epub 2015 Oct 22.

29.
30.

Thrombin promotes sustained signaling and inflammatory gene expression through the CDC25 and Ras-associating domains of phospholipase Cϵ.

Dusaban SS, Kunkel MT, Smrcka AV, Brown JH.

J Biol Chem. 2015 Oct 30;290(44):26776-83. doi: 10.1074/jbc.M115.676098. Epub 2015 Sep 8.

31.

Human domination of the biosphere: Rapid discharge of the earth-space battery foretells the future of humankind.

Schramski JR, Gattie DK, Brown JH.

Proc Natl Acad Sci U S A. 2015 Aug 4;112(31):9511-7. doi: 10.1073/pnas.1508353112. Epub 2015 Jul 15.

32.

Face processing in Williams syndrome is already atypical in infancy.

D'Souza D, Cole V, Farran EK, Brown JH, Humphreys K, Howard J, Rodic M, Dekker TM, D'Souza H, Karmiloff-Smith A.

Front Psychol. 2015 Jun 15;6:760. doi: 10.3389/fpsyg.2015.00760. eCollection 2015.

33.

CaMKIIδ mediates β-adrenergic effects on RyR2 phosphorylation and SR Ca(2+) leak and the pathophysiological response to chronic β-adrenergic stimulation.

Grimm M, Ling H, Willeford A, Pereira L, Gray CB, Erickson JR, Sarma S, Respress JL, Wehrens XH, Bers DM, Brown JH.

J Mol Cell Cardiol. 2015 Aug;85:282-91. doi: 10.1016/j.yjmcc.2015.06.007. Epub 2015 Jun 14.

34.

The First 50 Years of Molecular Pharmacology.

Brown JH, Catterall WA, Conn PJ, Cull-Candy SG, Dingledine R, Harden TK, Insel PA, Milligan G, Traynelis SF.

Mol Pharmacol. 2015 Jul;88(1):139-40. doi: 10.1124/mol.115.099564. Epub 2015 May 5. Review.

35.

G Protein-Coupled Receptor and RhoA-Stimulated Transcriptional Responses: Links to Inflammation, Differentiation, and Cell Proliferation.

Yu OM, Brown JH.

Mol Pharmacol. 2015 Jul;88(1):171-80. doi: 10.1124/mol.115.097857. Epub 2015 Apr 22. Review.

36.

Why are there so many species in the tropics?

Brown JH.

J Biogeogr. 2014 Jan;41(1):8-22.

37.

Metabolic theory predicts whole-ecosystem properties.

Schramski JR, Dell AI, Grady JM, Sibly RM, Brown JH.

Proc Natl Acad Sci U S A. 2015 Feb 24;112(8):2617-22. doi: 10.1073/pnas.1423502112. Epub 2015 Jan 26.

38.

Pillars article: three-dimensional structure of the human class II histocompatibility antigen HLA-DR1. Nature. 1993. 364: 33-39.

Brown JH, Jardetzky TS, Gorga JC, Stern LJ, Urban RG, Strominger JL, Wiley DC.

J Immunol. 2015 Jan 1;194(1):5-11. No abstract available.

39.

PLCε mediated sustained signaling pathways.

Dusaban SS, Brown JH.

Adv Biol Regul. 2015 Jan;57:17-23. doi: 10.1016/j.jbior.2014.09.014. Epub 2014 Oct 5. Review.

40.

CaMKII confirms its promise in ischaemic heart disease.

van Oort RJ, Brown JH, Westenbrink BD.

Eur J Heart Fail. 2014 Dec;16(12):1268-9. doi: 10.1002/ejhf.204. No abstract available.

41.

Induction of the matricellular protein CCN1 through RhoA and MRTF-A contributes to ischemic cardioprotection.

Zhao X, Ding EY, Yu OM, Xiang SY, Tan-Sah VP, Yung BS, Hedgpeth J, Neubig RR, Lau LF, Brown JH, Miyamoto S.

J Mol Cell Cardiol. 2014 Oct;75:152-61. doi: 10.1016/j.yjmcc.2014.07.017. Epub 2014 Aug 8.

42.

CaMKII-dependent phosphorylation of cardiac ryanodine receptors regulates cell death in cardiac ischemia/reperfusion injury.

Di Carlo MN, Said M, Ling H, Valverde CA, De Giusti VC, Sommese L, Palomeque J, Aiello EA, Skapura DG, Rinaldi G, Respress JL, Brown JH, Wehrens XH, Salas MA, Mattiazzi A.

J Mol Cell Cardiol. 2014 Sep;74:274-83. doi: 10.1016/j.yjmcc.2014.06.004. Epub 2014 Jun 17.

43.

Macroecology Meets Macroeconomics: Resource Scarcity and Global Sustainability.

Brown JH, Burger JR, Burnside WR, Chang M, Davidson AD, Fristoe TS, Hamilton MJ, Hammond ST, Kodric-Brown A, Mercado-Silva N, Nekola JC, Okie JG.

Ecol Eng. 2014 Apr 1;65:24-32.

44.

In vivo selective expression of thyroid hormone receptor α1 in endothelial cells attenuates myocardial injury in experimental myocardial infarction in mice.

Suarez J, Wang H, Scott BT, Ling H, Makino A, Swanson E, Brown JH, Suarez JA, Feinstein S, Diaz-Juarez J, Dillmann WH.

Am J Physiol Regul Integr Comp Physiol. 2014 Aug 1;307(3):R340-6. doi: 10.1152/ajpregu.00449.2013. Epub 2014 May 21.

45.

Why marine islands are farther apart in the tropics.

Brown JH.

Am Nat. 2014 Jun;183(6):842-6. doi: 10.1086/676015. Epub 2014 May 1.

PMID:
24823826
46.

The Ras-related protein, Rap1A, mediates thrombin-stimulated, integrin-dependent glioblastoma cell proliferation and tumor growth.

Sayyah J, Bartakova A, Nogal N, Quilliam LA, Stupack DG, Brown JH.

J Biol Chem. 2014 Jun 20;289(25):17689-98. doi: 10.1074/jbc.M113.536227. Epub 2014 May 1.

47.

Patterns of maximum body size evolution in Cenozoic land mammals: eco-evolutionary processes and abiotic forcing.

Saarinen JJ, Boyer AG, Brown JH, Costa DP, Ernest SK, Evans AR, Fortelius M, Gittleman JL, Hamilton MJ, Harding LE, Lintulaakso K, Lyons SK, Okie JG, Sibly RM, Stephens PR, Theodor J, Uhen MD, Smith FA.

Proc Biol Sci. 2014 Apr 16;281(1784):20132049. doi: 10.1098/rspb.2013.2049. Print 2014 Jun 7.

48.

Striatins contain a noncanonical coiled coil that binds protein phosphatase 2A A subunit to form a 2:2 heterotetrameric core of striatin-interacting phosphatase and kinase (STRIPAK) complex.

Chen C, Shi Z, Zhang W, Chen M, He F, Zhang Z, Wang Y, Feng M, Wang W, Zhao Y, Brown JH, Jiao S, Zhou Z.

J Biol Chem. 2014 Apr 4;289(14):9651-61. doi: 10.1074/jbc.M113.529297. Epub 2014 Feb 18.

49.

A new non-canonical pathway of Gα(q) protein regulating mitochondrial dynamics and bioenergetics.

Benincá C, Planagumà J, de Freitas Shuck A, Acín-Perez R, Muñoz JP, de Almeida MM, Brown JH, Murphy AN, Zorzano A, Enríquez JA, Aragay AM.

Cell Signal. 2014 May;26(5):1135-46. doi: 10.1016/j.cellsig.2014.01.009. Epub 2014 Jan 18.

50.

How body mass and lifestyle affect juvenile biomass production in placental mammals.

Sibly RM, Grady JM, Venditti C, Brown JH.

Proc Biol Sci. 2014 Jan 8;281(1777):20132818. doi: 10.1098/rspb.2013.2818. Print 2014 Feb 22.

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