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Items: 1 to 50 of 54

1.

Mechanistic reconstruction of glycoprotein secretion through monitoring of intracellular N-glycan processing.

Arigoni-Affolter I, Scibona E, Lin CW, Brühlmann D, Souquet J, Broly H, Aebi M.

Sci Adv. 2019 Nov 27;5(11):eaax8930. doi: 10.1126/sciadv.aax8930. eCollection 2019 Nov.

2.

Understanding mAb aggregation during low pH viral inactivation and subsequent neutralization.

Wälchli R, Ressurreição M, Vogg S, Feidl F, Angelo J, Xu X, Ghose S, Jian Li Z, Le Saoût X, Souquet J, Broly H, Morbidelli M.

Biotechnol Bioeng. 2019 Nov 30. doi: 10.1002/bit.27237. [Epub ahead of print]

PMID:
31784982
3.

No interactions between heparin and atacicept, an antagonist of B cell survival cytokines.

Kowalczyk-Quintas C, Willen D, Willen L, Golob M, Schuepbach-Mallepell S, Peter B, Eslami M, Vigolo M, Broly H, Samy E, Yalkinoglu Ö, Schneider P.

Br J Pharmacol. 2019 Oct;176(20):4019-4033. doi: 10.1111/bph.14811. Epub 2019 Oct 15.

4.

Monitoring of antibody glycosylation pattern based on microarray MALDI-TOF mass spectrometry.

Hajduk J, Wolf M, Steinhoff R, Karst D, Souquet J, Broly H, Morbidelli M, Zenobi R.

J Biotechnol. 2019 Aug 20;302:77-84. doi: 10.1016/j.jbiotec.2019.06.306. Epub 2019 Jun 28.

PMID:
31260704
5.

Perfusion cell culture for the production of conjugated recombinant fusion proteins reduces clipping and quality heterogeneity compared to batch-mode processes.

Bielser JM, Chappuis L, Xiao Y, Souquet J, Broly H, Morbidelli M.

J Biotechnol. 2019 Aug 20;302:26-31. doi: 10.1016/j.jbiotec.2019.06.006. Epub 2019 Jun 15.

PMID:
31207262
6.

A new flow cell and chemometric protocol for implementing in-line Raman spectroscopy in chromatography.

Feidl F, Garbellini S, Vogg S, Sokolov M, Souquet J, Broly H, Butté A, Morbidelli M.

Biotechnol Prog. 2019 Sep;35(5):e2847. doi: 10.1002/btpr.2847. Epub 2019 May 28.

PMID:
31099991
7.

Process design and development of a mammalian cell perfusion culture in shake-tube and benchtop bioreactors.

Wolf MKF, Müller A, Souquet J, Broly H, Morbidelli M.

Biotechnol Bioeng. 2019 Aug;116(8):1973-1985. doi: 10.1002/bit.26999. Epub 2019 May 19.

PMID:
31038199
8.

Experimental Evaluation of the Impact of Intrinsic Process Parameters on the Performance of a Continuous Chromatographic Polishing Unit (MCSGP).

Vogg S, Ulmer N, Souquet J, Broly H, Morbidelli M.

Biotechnol J. 2019 Jul;14(7):e1800732. doi: 10.1002/biot.201800732. Epub 2019 May 2.

PMID:
30927513
9.

Semi-continuous scale-down models for clone and operating parameter screening in perfusion bioreactors.

Bielser JM, Domaradzki J, Souquet J, Broly H, Morbidelli M.

Biotechnol Prog. 2019 May;35(3):e2790. doi: 10.1002/btpr.2790. Epub 2019 Mar 13.

PMID:
30773840
10.

Generation of site-distinct N-glycan variants for in vitro bioactivity testing.

Brühlmann D, Vuillemin T, Satwekar A, Galano E, Palmese A, D'Angelo A, Manco Z, Souquet J, Broly H, Sauer M, Hemberger J, Jordan M.

Biotechnol Bioeng. 2019 May;116(5):1017-1028. doi: 10.1002/bit.26930. Epub 2019 Feb 27.

PMID:
30659587
11.

Development of a shake tube-based scale-down model for perfusion cultures.

Wolf MKF, Lorenz V, Karst DJ, Souquet J, Broly H, Morbidelli M.

Biotechnol Bioeng. 2018 Nov;115(11):2703-2713. doi: 10.1002/bit.26804. Epub 2018 Sep 10.

PMID:
30039852
12.

Improved Performance in Mammalian Cell Perfusion Cultures by Growth Inhibition.

Wolf MKF, Closet A, Bzowska M, Bielser JM, Souquet J, Broly H, Morbidelli M.

Biotechnol J. 2019 Feb;14(2):e1700722. doi: 10.1002/biot.201700722. Epub 2018 Jun 4.

PMID:
29781256
13.

Perfusion mammalian cell culture for recombinant protein manufacturing - A critical review.

Bielser JM, Wolf M, Souquet J, Broly H, Morbidelli M.

Biotechnol Adv. 2018 Jul - Aug;36(4):1328-1340. doi: 10.1016/j.biotechadv.2018.04.011. Epub 2018 May 5. Review.

14.

Proteomic analysis of micro-scale bioreactors as scale-down model for a mAb producing CHO industrial fed-batch platform.

Bertrand V, Vogg S, Villiger TK, Stettler M, Broly H, Soos M, Morbidelli M.

J Biotechnol. 2018 Aug 10;279:27-36. doi: 10.1016/j.jbiotec.2018.04.015. Epub 2018 Apr 30.

PMID:
29719200
15.

Implementation of Plate Imaging for Demonstration of Monoclonality in Biologics Manufacturing Development.

Zingaro K, Shaw D, Carson J, Mayer-Bartschmid A, Bender C, Alves C, Mcvey D, Qian NX, Wei Q, Laird MW, Zhu Y, Emmins R, Follit JA, Porter A, Racher AJ, Milne S, Carubelli I, Du Z, Khatri A, Failly M, Broly H, Lee F, Reeser M, Spidel J, Anderson K, Demaria C, Di-Carlo J, Gill J, Lundquist A, Kumar SR, Gill T.

PDA J Pharm Sci Technol. 2018 Jul-Aug;72(4):438-450. doi: 10.5731/pdajpst.2018.008789. Epub 2018 Apr 18.

PMID:
29669815
16.

Sequential Multivariate Cell Culture Modeling at Multiple Scales Supports Systematic Shaping of a Monoclonal Antibody Toward a Quality Target.

Sokolov M, Morbidelli M, Butté A, Souquet J, Broly H.

Biotechnol J. 2018 Apr;13(4):e1700461. doi: 10.1002/biot.201700461. Epub 2018 Jan 30.

PMID:
29283215
17.

Glycosylation flux analysis reveals dynamic changes of intracellular glycosylation flux distribution in Chinese hamster ovary fed-batch cultures.

Hutter S, Villiger TK, Brühlmann D, Stettler M, Broly H, Soos M, Gunawan R.

Metab Eng. 2017 Sep;43(Pt A):9-20. doi: 10.1016/j.ymben.2017.07.005. Epub 2017 Jul 25.

PMID:
28754360
18.

Enhanced process understanding and multivariate prediction of the relationship between cell culture process and monoclonal antibody quality.

Sokolov M, Ritscher J, MacKinnon N, Souquet J, Broly H, Morbidelli M, Butté A.

Biotechnol Prog. 2017 Sep;33(5):1368-1380. doi: 10.1002/btpr.2502. Epub 2017 Jun 21.

PMID:
28556619
19.

Cell culture media supplemented with raffinose reproducibly enhances high mannose glycan formation.

Brühlmann D, Muhr A, Parker R, Vuillemin T, Bucsella B, Kalman F, Torre S, La Neve F, Lembo A, Haas T, Sauer M, Souquet J, Broly H, Hemberger J, Jordan M.

J Biotechnol. 2017 Jun 20;252:32-42. doi: 10.1016/j.jbiotec.2017.04.026. Epub 2017 Apr 29.

PMID:
28465212
20.

Modulation and modeling of monoclonal antibody N-linked glycosylation in mammalian cell perfusion reactors.

Karst DJ, Scibona E, Serra E, Bielser JM, Souquet J, Stettler M, Broly H, Soos M, Morbidelli M, Villiger TK.

Biotechnol Bioeng. 2017 Sep;114(9):1978-1990. doi: 10.1002/bit.26315. Epub 2017 May 23.

PMID:
28409838
21.

Evaluation of several protein a resins for application to multicolumn chromatography for the rapid purification of fed-batch bioreactors.

Hilbold NJ, Le Saoût X, Valery E, Muhr L, Souquet J, Lamproye A, Broly H.

Biotechnol Prog. 2017 Jul;33(4):941-953. doi: 10.1002/btpr.2465. Epub 2017 Apr 26.

PMID:
28371561
22.

Parallel experimental design and multivariate analysis provides efficient screening of cell culture media supplements to improve biosimilar product quality.

Brühlmann D, Sokolov M, Butté A, Sauer M, Hemberger J, Souquet J, Broly H, Jordan M.

Biotechnol Bioeng. 2017 Jul;114(7):1448-1458. doi: 10.1002/bit.26269. Epub 2017 Mar 14.

PMID:
28197999
23.

Robust factor selection in early cell culture process development for the production of a biosimilar monoclonal antibody.

Sokolov M, Ritscher J, MacKinnon N, Bielser JM, Brühlmann D, Rothenhäusler D, Thanei G, Soos M, Stettler M, Souquet J, Broly H, Morbidelli M, Butté A.

Biotechnol Prog. 2017 Jan;33(1):181-191. doi: 10.1002/btpr.2374. Epub 2016 Oct 31.

PMID:
27689949
24.

Controlling the time evolution of mAb N-linked glycosylation - Part II: Model-based predictions.

Villiger TK, Scibona E, Stettler M, Broly H, Morbidelli M, Soos M.

Biotechnol Prog. 2016 Sep;32(5):1135-1148. doi: 10.1002/btpr.2315. Epub 2016 Jul 1.

PMID:
27273889
25.

Controlling the time evolution of mAb N-linked glycosylation, Part I: Microbioreactor experiments.

Villiger TK, Roulet A, Périlleux A, Stettler M, Broly H, Morbidelli M, Soos M.

Biotechnol Prog. 2016 Sep;32(5):1123-1134. doi: 10.1002/btpr.2305. Epub 2016 Jun 27.

PMID:
27254475
26.

High-throughput profiling of nucleotides and nucleotide sugars to evaluate their impact on antibody N-glycosylation.

Villiger TK, Steinhoff RF, Ivarsson M, Solacroup T, Stettler M, Broly H, Krismer J, Pabst M, Zenobi R, Morbidelli M, Soos M.

J Biotechnol. 2016 Jul 10;229:3-12. doi: 10.1016/j.jbiotec.2016.04.039. Epub 2016 Apr 27.

PMID:
27131894
27.

Pilot-scale verification of maximum tolerable hydrodynamic stress for mammalian cell culture.

Neunstoecklin B, Villiger TK, Lucas E, Stettler M, Broly H, Morbidelli M, Soos M.

Appl Microbiol Biotechnol. 2016 Apr;100(8):3489-98. doi: 10.1007/s00253-015-7193-x. Epub 2015 Dec 5.

PMID:
26637424
28.

Screening and assessment of performance and molecule quality attributes of industrial cell lines across different fed-batch systems.

Rouiller Y, Bielser JM, Brühlmann D, Jordan M, Broly H, Stettler M.

Biotechnol Prog. 2016 Jan-Feb;32(1):160-70. doi: 10.1002/btpr.2186. Epub 2015 Nov 3.

PMID:
26489036
29.

Fingerprint detection and process prediction by multivariate analysis of fed-batch monoclonal antibody cell culture data.

Sokolov M, Soos M, Neunstoecklin B, Morbidelli M, Butté A, Leardi R, Solacroup T, Stettler M, Broly H.

Biotechnol Prog. 2015 Nov-Dec;31(6):1633-44. doi: 10.1002/btpr.2174. Epub 2015 Oct 7.

PMID:
26399784
30.

Reciprocal Translocation Observed in End-of-Production Cells of a Commercial CHO-Based Process.

Rouiller Y, Kleuser B, Toso E, Palinksy W, Rossi M, Rossatto P, Barberio D, Broly H.

PDA J Pharm Sci Technol. 2015 Jul-Aug;69(4):540-52. doi: 10.5731/pdajpst.2015.01063.

PMID:
26242789
31.

Tailoring recombinant protein quality by rational media design.

Brühlmann D, Jordan M, Hemberger J, Sauer M, Stettler M, Broly H.

Biotechnol Prog. 2015 May-Jun;31(3):615-29. doi: 10.1002/btpr.2089. Epub 2015 Apr 29. Review.

PMID:
25864704
32.

Determination of the maximum operating range of hydrodynamic stress in mammalian cell culture.

Neunstoecklin B, Stettler M, Solacroup T, Broly H, Morbidelli M, Soos M.

J Biotechnol. 2015 Jan 20;194:100-9. doi: 10.1016/j.jbiotec.2014.12.003. Epub 2014 Dec 18.

PMID:
25529344
33.

Modulation of mAb quality attributes using microliter scale fed-batch cultures.

Rouiller Y, Périlleux A, Vesin MN, Stettler M, Jordan M, Broly H.

Biotechnol Prog. 2014 May-Jun;30(3):571-83. doi: 10.1002/btpr.1921. Epub 2014 May 10.

PMID:
24777991
34.

A high-throughput media design approach for high performance mammalian fed-batch cultures.

Rouiller Y, Périlleux A, Collet N, Jordan M, Stettler M, Broly H.

MAbs. 2013 May-Jun;5(3):501-11. doi: 10.4161/mabs.23942. Epub 2013 Apr 5.

35.

Application of the quality by design approach to the drug substance manufacturing process of an Fc fusion protein: towards a global multi-step design space.

Eon-duval A, Valax P, Solacroup T, Broly H, Gleixner R, Strat CL, Sutter J.

J Pharm Sci. 2012 Oct;101(10):3604-18. doi: 10.1002/jps.23273. Epub 2012 Jul 20.

PMID:
22821774
36.

Cell culture medium improvement by rigorous shuffling of components using media blending.

Jordan M, Voisard D, Berthoud A, Tercier L, Kleuser B, Baer G, Broly H.

Cytotechnology. 2013 Jan;65(1):31-40. doi: 10.1007/s10616-012-9462-1. Epub 2012 Jun 14.

37.

Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity.

Zagari F, Jordan M, Stettler M, Broly H, Wurm FM.

N Biotechnol. 2013 Jan 25;30(2):238-45. doi: 10.1016/j.nbt.2012.05.021. Epub 2012 Jun 6.

PMID:
22683938
38.

Effect of hydrocortisone on the production and glycosylation of an Fc-fusion protein in CHO cell cultures.

Rouiller Y, Périlleux A, Marsaut M, Stettler M, Vesin MN, Broly H.

Biotechnol Prog. 2012 May-Jun;28(3):803-13. doi: 10.1002/btpr.1530. Epub 2012 Apr 26.

PMID:
22535835
39.

Quality attributes of recombinant therapeutic proteins: an assessment of impact on safety and efficacy as part of a quality by design development approach.

Eon-Duval A, Broly H, Gleixner R.

Biotechnol Prog. 2012 May-Jun;28(3):608-22. doi: 10.1002/btpr.1548. Epub 2012 May 2. Review.

PMID:
22473974
40.

Application of Quality by Design to the characterization of the cell culture process of an Fc-Fusion protein.

Rouiller Y, Solacroup T, Deparis V, Barbafieri M, Gleixner R, Broly H, Eon-Duval A.

Eur J Pharm Biopharm. 2012 Jun;81(2):426-37. doi: 10.1016/j.ejpb.2012.02.018. Epub 2012 Mar 9.

PMID:
22426134
41.

Minimizing hydrodynamic stress in mammalian cell culture through the lobed Taylor-Couette bioreactor.

Sorg R, Tanzeglock T, Soos M, Morbidelli M, Périlleux A, Solacroup T, Broly H.

Biotechnol J. 2011 Dec;6(12):1504-15. doi: 10.1002/biot.201000477. Epub 2011 Aug 15.

PMID:
21766459
42.

Degradation of an Fc-fusion recombinant protein by host cell proteases: Identification of a CHO cathepsin D protease.

Robert F, Bierau H, Rossi M, Agugiaro D, Soranzo T, Broly H, Mitchell-Logean C.

Biotechnol Bioeng. 2009 Dec 15;104(6):1132-41. doi: 10.1002/bit.22494.

PMID:
19655395
43.

Influenza virus host response of C57Bl/6 mice treated with TACI-Ig.

Roque R, Ponce R, Burleson F, Cabrit M, Broly H, Rogge M.

Immunopharmacol Immunotoxicol. 2006;28(1):13-32.

PMID:
16684665
44.

Restricted use of cationic germline V(H) gene segments in human Rh(D) red cell antibodies.

Boucher G, Broly H, Lemieux R.

Blood. 1997 May 1;89(9):3277-86.

PMID:
9129033
45.

Use of hu-IgG-SCID mice to evaluate the in vivo stability of human monoclonal IgG antibodies.

Bazin R, Boucher G, Monier G, Chevrier MC, Verrette S, Broly H, Lemieux R.

J Immunol Methods. 1994 Jun 24;172(2):209-17.

PMID:
7518484
46.

Human monoclonal anti-D-induced clearance of human D-positive red cells in a chimpanzee model.

Blancher A, Socha WW, Roubinet F, Rowe AW, Broly H, Byrne P, Holuigue M, Bouzidi A, Huart JJ, Ruffié J.

Vox Sang. 1993;65(1):47-54.

PMID:
8362514
47.

Direct agglutination of weak D red cells by tetramolecular complexes containing monoclonal IgG anti-D.

Lemieux R, Verrette S, Broly H, Perron S.

Vox Sang. 1993;65(2):141-5.

PMID:
7692674
48.

Detection of HIV-1 infections by PCR: evaluation in a seropositive subject population.

Bournique B, Akar A, Broly H, Ajana F, Counis R, Scholler R.

Mol Cell Probes. 1992 Dec;6(6):443-50.

PMID:
1480185
49.

Survival of human monoclonal anti-Rho (D) antibodies in the rhesus monkey.

Blancher A, Roubinet F, Blanchard N, Byrne P, Broly H, Ducos J, Socha WW, Ruffie J.

J Med Primatol. 1992 Aug;21(6):328-31.

PMID:
1338470
50.

[Characterization and validation of a human anti-C monoclonal antibody].

Mannessier L, Horbez C, Broly H.

Rev Fr Transfus Hemobiol. 1991 Oct;34(5):403-8. French.

PMID:
1772524

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