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Items: 1 to 50 of 78

1.

Comparison of high-throughput biophysical methods to identify stabilizing excipients for a model IgG2 monoclonal antibody: conformational stability and kinetic aggregation measurements.

Cheng W, Joshi SB, He F, Brems DN, He B, Kerwin BA, Volkin DB, Middaugh CR.

J Pharm Sci. 2012 May;101(5):1701-20. doi: 10.1002/jps.23076. Epub 2012 Feb 9.

PMID:
22323186
2.

Interactions between Therapeutic Proteins and Acrylic Acid Leachable.

Liu D, Nashed-Samuel Y, Bondarenko PV, Brems DN, Ren D.

PDA J Pharm Sci Technol. 2012 Jan-Feb;66(1):12-9. doi: 10.5731/pdajpst.2012.00803.

PMID:
22307658
3.

Determination of the dipole moments of RNAse SA wild type and a basic mutant.

Chari R, Singh SN, Yadav S, Brems DN, Kalonia DS.

Proteins. 2012 Apr;80(4):1041-52. doi: 10.1002/prot.24006. Epub 2011 Dec 28.

PMID:
22213585
4.

Effective charge measurements reveal selective and preferential accumulation of anions, but not cations, at the protein surface in dilute salt solutions.

Gokarn YR, Fesinmeyer RM, Saluja A, Razinkov V, Chase SF, Laue TM, Brems DN.

Protein Sci. 2011 Mar;20(3):580-7. doi: 10.1002/pro.591.

5.

Diffusion and sedimentation interaction parameters for measuring the second virial coefficient and their utility as predictors of protein aggregation.

Saluja A, Fesinmeyer RM, Hogan S, Brems DN, Gokarn YR.

Biophys J. 2010 Oct 20;99(8):2657-65. doi: 10.1016/j.bpj.2010.08.020.

6.

High-throughput dynamic light scattering method for measuring viscosity of concentrated protein solutions.

He F, Becker GW, Litowski JR, Narhi LO, Brems DN, Razinkov VI.

Anal Biochem. 2010 Apr 1;399(1):141-3. doi: 10.1016/j.ab.2009.12.003. Epub 2009 Dec 6.

PMID:
19995543
7.

Denaturant-dependent conformational changes in a beta-trefoil protein: global and residue-specific aspects of an equilibrium denaturation process.

Latypov RF, Liu D, Jacob J, Harvey TS, Bondarenko PV, Kleemann GR, Brems DN, Raibekas AA.

Biochemistry. 2009 Nov 24;48(46):10934-47. doi: 10.1021/bi901570k.

PMID:
19839644
8.

An improved trypsin digestion method minimizes digestion-induced modifications on proteins.

Ren D, Pipes GD, Liu D, Shih LY, Nichols AC, Treuheit MJ, Brems DN, Bondarenko PV.

Anal Biochem. 2009 Sep 1;392(1):12-21. doi: 10.1016/j.ab.2009.05.018. Epub 2009 May 18.

PMID:
19457431
9.

Silicone oil- and agitation-induced aggregation of a monoclonal antibody in aqueous solution.

Thirumangalathu R, Krishnan S, Ricci MS, Brems DN, Randolph TW, Carpenter JF.

J Pharm Sci. 2009 Sep;98(9):3167-81. doi: 10.1002/jps.21719.

10.

Ion-specific modulation of protein interactions: anion-induced, reversible oligomerization of a fusion protein.

Gokarn YR, Fesinmeyer RM, Saluja A, Cao S, Dankberg J, Goetze A, Remmele RL Jr, Narhi LO, Brems DN.

Protein Sci. 2009 Jan;18(1):169-79. doi: 10.1002/pro.20.

11.

Effect of ions on agitation- and temperature-induced aggregation reactions of antibodies.

Fesinmeyer RM, Hogan S, Saluja A, Brych SR, Kras E, Narhi LO, Brems DN, Gokarn YR.

Pharm Res. 2009 Apr;26(4):903-13. doi: 10.1007/s11095-008-9792-z. Epub 2008 Dec 23.

PMID:
19104916
12.

Degradation products analysis of an Fc fusion protein using LC/MS methods.

Ren D, Ratnaswamy G, Beierle J, Treuheit MJ, Brems DN, Bondarenko PV.

Int J Biol Macromol. 2009 Jan 1;44(1):81-5. doi: 10.1016/j.ijbiomac.2008.10.006. Epub 2008 Oct 30.

PMID:
19014964
13.

Anion binding mediated precipitation of a peptibody.

Saluja A, Crampton S, Kras E, Fesinmeyer RM, Remmele RL Jr, Narhi LO, Brems DN, Gokarn YR.

Pharm Res. 2009 Jan;26(1):152-60. doi: 10.1007/s11095-008-9722-0. Epub 2008 Sep 27.

PMID:
18820999
14.

Structure and stability changes of human IgG1 Fc as a consequence of methionine oxidation.

Liu D, Ren D, Huang H, Dankberg J, Rosenfeld R, Cocco MJ, Li L, Brems DN, Remmele RL Jr.

Biochemistry. 2008 May 6;47(18):5088-100. doi: 10.1021/bi702238b. Epub 2008 Apr 12.

PMID:
18407665
15.

Isomerization of a single aspartyl residue of anti-epidermal growth factor receptor immunoglobulin gamma2 antibody highlights the role avidity plays in antibody activity.

Rehder DS, Chelius D, McAuley A, Dillon TM, Xiao G, Crouse-Zeineddini J, Vardanyan L, Perico N, Mukku V, Brems DN, Matsumura M, Bondarenko PV.

Biochemistry. 2008 Feb 26;47(8):2518-30. doi: 10.1021/bi7018223. Epub 2008 Jan 31.

PMID:
18232715
16.

Contributions of a disulfide bond to the structure, stability, and dimerization of human IgG1 antibody CH3 domain.

McAuley A, Jacob J, Kolvenbach CG, Westland K, Lee HJ, Brych SR, Rehder D, Kleemann GR, Brems DN, Matsumura M.

Protein Sci. 2008 Jan;17(1):95-106.

17.

Assignment of backbone (1)H, (13)C and (15)N resonances of human IgG1 Fc (51.4 kDa).

Liu D, Cocco MJ, Rosenfied R, Lewis JK, Ren D, Li L, Remmele RL Jr, Brems DN.

Biomol NMR Assign. 2007 Dec;1(2):233-5. doi: 10.1007/s12104-007-9065-5. Epub 2007 Nov 22.

PMID:
19636873
18.

Self-buffering antibody formulations.

Gokarn YR, Kras E, Nodgaard C, Dharmavaram V, Fesinmeyer RM, Hultgen H, Brych S, Remmele RL Jr, Brems DN, Hershenson S.

J Pharm Sci. 2008 Aug;97(8):3051-66.

PMID:
18023013
19.

Reversed-phase liquid chromatography of immunoglobulin G molecules and their fragments with the diphenyl column.

Ren D, Pipes GD, Hambly DM, Bondarenko PV, Treuheit MJ, Brems DN, Gadgil HS.

J Chromatogr A. 2007 Dec 14;1175(1):63-8. Epub 2007 Oct 10.

PMID:
17980377
20.

Assignment of 1H, 13C and 15N resonances of the reduced human IgG1 C(H)3 domain.

Liu D, Cocco M, Matsumura M, Ren D, Becker B, Remmele RL Jr, Brems DN.

Biomol NMR Assign. 2007 Jul;1(1):93-4. doi: 10.1007/s12104-007-9026-z. Epub 2007 Jul 4.

PMID:
19636836
21.

Oxidation of methionine residues in recombinant human interleukin-1 receptor antagonist: implications of conformational stability on protein oxidation kinetics.

Thirumangalathu R, Krishnan S, Bondarenko P, Speed-Ricci M, Randolph TW, Carpenter JF, Brems DN.

Biochemistry. 2007 May 29;46(21):6213-24. Epub 2007 May 5.

PMID:
17480058
22.

Biophysical characterization of structural properties and folding of interleukin-1 receptor antagonist.

Latypov RF, Harvey TS, Liu D, Bondarenko PV, Kohno T, Fachini RA 2nd, Rosenfeld RD, Ketchem RR, Brems DN, Raibekas AA.

J Mol Biol. 2007 May 11;368(4):1187-201. Epub 2007 Feb 22.

PMID:
17391700
23.

Comparative oxidation studies of methionine residues reflect a structural effect on chemical kinetics in rhG-CSF.

Pan B, Abel J, Ricci MS, Brems DN, Wang DI, Trout BL.

Biochemistry. 2006 Dec 26;45(51):15430-43.

PMID:
17176065
24.

Effects of pH, temperature, and sucrose on benzyl alcohol-induced aggregation of recombinant human granulocyte colony stimulating factor.

Thirumangalathu R, Krishnan S, Brems DN, Randolph TW, Carpenter JF.

J Pharm Sci. 2006 Jul;95(7):1480-97.

PMID:
16729274
25.

Modulation of protein aggregation by polyethylene glycol conjugation: GCSF as a case study.

Rajan RS, Li T, Aras M, Sloey C, Sutherland W, Arai H, Briddell R, Kinstler O, Lueras AM, Zhang Y, Yeghnazar H, Treuheit M, Brems DN.

Protein Sci. 2006 May;15(5):1063-75. Epub 2006 Apr 5.

26.

Active dimer of Epratuzumab provides insight into the complex nature of an antibody aggregate.

Remmele RL Jr, Callahan WJ, Krishnan S, Zhou L, Bondarenko PV, Nichols AC, Kleemann GR, Pipes GD, Park S, Fodor S, Kras E, Brems DN.

J Pharm Sci. 2006 Jan;95(1):126-45.

PMID:
16315222
27.

Aggregation of granulocyte-colony stimulating factor in vitro involves a conformationally altered monomeric state.

Raso SW, Abel J, Barnes JM, Maloney KM, Pipes G, Treuheit MJ, King J, Brems DN.

Protein Sci. 2005 Sep;14(9):2246-57.

28.

Charge-charge interactions in the denatured state influence the folding kinetics of ribonuclease Sa.

Trefethen JM, Pace CN, Scholtz JM, Brems DN.

Protein Sci. 2005 Jul;14(7):1934-8. Epub 2005 Jun 3.

29.

Effects of excipients on the hydrogen peroxide-induced oxidation of methionine residues in granulocyte colony-stimulating factor.

Yin J, Chu JW, Ricci MS, Brems DN, Wang DI, Trout BL.

Pharm Res. 2005 Jan;22(1):141-7.

PMID:
15771240
31.
32.

A comprehensive picture of non-site specific oxidation of methionine residues by peroxides in protein pharmaceuticals.

Chu JW, Yin J, Brooks BR, Wang DI, Ricci MS, Brems DN, Trout BL.

J Pharm Sci. 2004 Dec;93(12):3096-102.

PMID:
15514984
33.

pH Dependence of structural stability of interleukin-2 and granulocyte colony-stimulating factor.

Ricci MS, Sarkar CA, Fallon EM, Lauffenburger DA, Brems DN.

Protein Sci. 2003 May;12(5):1030-8.

34.

The kinetics of G-CSF folding.

Brems DN.

Protein Sci. 2002 Oct;11(10):2504-11.

35.

Inverse relationship of protein concentration and aggregation.

Treuheit MJ, Kosky AA, Brems DN.

Pharm Res. 2002 Apr;19(4):511-6.

PMID:
12033388
36.
37.

PEGylation prevents the N-terminal degradation of megakaryocyte growth and development factor.

Guerra PI, Acklin C, Kosky AA, Davis JM, Treuheit MJ, Brems DN.

Pharm Res. 1998 Dec;15(12):1822-7.

PMID:
9892464
38.
39.

Physicochemical basis for the rapid time-action of LysB28ProB29-insulin: dissociation of a protein-ligand complex.

Bakaysa DL, Radziuk J, Havel HA, Brader ML, Li S, Dodd SW, Beals JM, Pekar AH, Brems DN.

Protein Sci. 1996 Dec;5(12):2521-31.

40.
42.

Characterization and stability of N-terminally PEGylated rhG-CSF.

Kinstler OB, Brems DN, Lauren SL, Paige AG, Hamburger JB, Treuheit MJ.

Pharm Res. 1996 Jul;13(7):996-1002.

PMID:
8842035
43.

Kinetic analysis of the folding of human growth hormone. Influence of disulfide bonds.

Youngman KM, Spencer DB, Brems DN, DeFelippis MR.

J Biol Chem. 1995 Aug 25;270(34):19816-22.

44.

Amide hydrogen exchange of the central B-chain helix within the T- and R-states of insulin hexamers.

Hardaway LA, Brems DN, Beals JM, MacKenzie NE.

Biochim Biophys Acta. 1994 Sep 21;1208(1):101-3.

PMID:
8086423
45.

Serine 228 is essential for catalytic activities of 85-kDa cytosolic phospholipase A2.

Sharp JD, Pickard RT, Chiou XG, Manetta JV, Kovacevic S, Miller JR, Varshavsky AD, Roberts EF, Strifler BA, Brems DN, et al.

J Biol Chem. 1994 Sep 16;269(37):23250-4.

46.

Secondary structure of amyloid beta peptide correlates with neurotoxic activity in vitro.

Simmons LK, May PC, Tomaselli KJ, Rydel RE, Fuson KS, Brigham EF, Wright S, Lieberburg I, Becker GW, Brems DN, et al.

Mol Pharmacol. 1994 Mar;45(3):373-9.

PMID:
8145724
47.

Equilibrium intermediates in the denaturation of human insulin and two monomeric insulin analogs.

Millican RL, Brems DN.

Biochemistry. 1994 Feb 8;33(5):1116-24.

PMID:
8110743
48.

Acid stabilization of insulin.

Bryant C, Spencer DB, Miller A, Bakaysa DL, McCune KS, Maple SR, Pekar AH, Brems DN.

Biochemistry. 1993 Aug 17;32(32):8075-82.

PMID:
8394123
49.

Evidence for a self-associating equilibrium intermediate during folding of human growth hormone.

DeFelippis MR, Alter LA, Pekar AH, Havel HA, Brems DN.

Biochemistry. 1993 Feb 16;32(6):1555-62.

PMID:
8431435
50.

Altering the association properties of insulin by amino acid replacement.

Brems DN, Alter LA, Beckage MJ, Chance RE, DiMarchi RD, Green LK, Long HB, Pekar AH, Shields JE, Frank BH.

Protein Eng. 1992 Sep;5(6):527-33.

PMID:
1438163

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