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Items: 1 to 50 of 92


An easy-to-perform, culture-free Campylobacter point-of-management assay for processing plant applications.

Mason MG, Blackall PJ, Botella JR, Templeton JM.

J Appl Microbiol. 2019 Nov 8. doi: 10.1111/jam.14509. [Epub ahead of print]


Nucleotide exchange-dependent and nucleotide exchange-independent functions of plant heterotrimeric GTP-binding proteins.

Maruta N, Trusov Y, Chakravorty D, Urano D, Assmann SM, Botella JR.

Sci Signal. 2019 Nov 5;12(606). pii: eaav9526. doi: 10.1126/scisignal.aav9526.


Perspectives on the Application of Genome-Editing Technologies in Crop Breeding.

Hua K, Zhang J, Botella JR, Ma C, Kong F, Liu B, Zhu JK.

Mol Plant. 2019 Aug 5;12(8):1047-1059. doi: 10.1016/j.molp.2019.06.009. Epub 2019 Jun 28. Review.


Beyond Light: Insights Into the Role of Constitutively Photomorphogenic1 in Plant Hormonal Signaling.

Wang W, Chen Q, Botella JR, Guo S.

Front Plant Sci. 2019 May 16;10:557. doi: 10.3389/fpls.2019.00557. eCollection 2019. Review.


Biosynthesis of DHGA12 and its roles in Arabidopsis seedling establishment.

Liu H, Guo S, Lu M, Zhang Y, Li J, Wang W, Wang P, Zhang J, Hu Z, Li L, Si L, Zhang J, Qi Q, Jiang X, Botella JR, Wang H, Song CP.

Nat Commun. 2019 Apr 16;10(1):1768. doi: 10.1038/s41467-019-09467-5.


Now for the hard ones: is there a limit on CRISPR genome editing in crops?

Botella JR.

J Exp Bot. 2019 Feb 5;70(3):734-737. doi: 10.1093/jxb/erz007. No abstract available.


A Highly Efficient Cell Division-Specific CRISPR/Cas9 System Generates Homozygous Mutants for Multiple Genes in Arabidopsis.

Feng Z, Zhang Z, Hua K, Gao X, Mao Y, Botella JR, Zhu JK.

Int J Mol Sci. 2018 Dec 7;19(12). pii: E3925. doi: 10.3390/ijms19123925.


Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression.

Long L, Guo DD, Gao W, Yang WW, Hou LP, Ma XN, Miao YC, Botella JR, Song CP.

Plant Methods. 2018 Oct 3;14:85. doi: 10.1186/s13007-018-0353-0. eCollection 2018.


Manipulating plant RNA-silencing pathways to improve the gene editing efficiency of CRISPR/Cas9 systems.

Mao Y, Yang X, Zhou Y, Zhang Z, Botella JR, Zhu JK.

Genome Biol. 2018 Sep 28;19(1):149. doi: 10.1186/s13059-018-1529-7.


A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum).

Li H, Li K, Guo Y, Guo J, Miao K, Botella JR, Song CP, Miao Y.

Plant Methods. 2018 Jun 22;14:50. doi: 10.1186/s13007-018-0319-2. eCollection 2018.


A simple and cost-effective method for screening of CRISPR/Cas9-induced homozygous/biallelic mutants.

Guo J, Li K, Jin L, Xu R, Miao K, Yang F, Qi C, Zhang L, Botella JR, Wang R, Miao Y.

Plant Methods. 2018 May 29;14:40. doi: 10.1186/s13007-018-0305-8. eCollection 2018.


Correction: Nucleic acid purification from plants, animals and microbes in under 30 seconds.

Zou Y, Mason MG, Wang Y, Wee E, Turni C, Blackall PJ, Trau M, Botella JR.

PLoS Biol. 2018 May 7;16(5):e1002630. doi: 10.1371/journal.pbio.1002630. eCollection 2018 May.


Advanced DNA-Based Point-of-Care Diagnostic Methods for Plant Diseases Detection.

Lau HY, Botella JR.

Front Plant Sci. 2017 Dec 6;8:2016. doi: 10.3389/fpls.2017.02016. eCollection 2017. Review.


Calcium-dependent protein kinases in cotton: insights into early plant responses to salt stress.

Gao W, Xu FC, Guo DD, Zhao JR, Liu J, Guo YW, Singh PK, Ma XN, Long L, Botella JR, Song CP.

BMC Plant Biol. 2018 Jan 17;18(1):15. doi: 10.1186/s12870-018-1230-8.


Generation of new glutinous rice by CRISPR/Cas9-targeted mutagenesis of the Waxy gene in elite rice varieties.

Zhang J, Zhang H, Botella JR, Zhu JK.

J Integr Plant Biol. 2018 May;60(5):369-375. doi: 10.1111/jipb.12620. Epub 2018 Jan 23.


Nucleic acid purification from plants, animals and microbes in under 30 seconds.

Zou Y, Mason MG, Wang Y, Wee E, Turni C, Blackall PJ, Trau M, Botella JR.

PLoS Biol. 2017 Nov 21;15(11):e2003916. doi: 10.1371/journal.pbio.2003916. eCollection 2017 Nov. Erratum in: PLoS Biol. 2018 May 7;16(5):e1002630.


VERNALIZATION1 Modulates Root System Architecture in Wheat and Barley.

Voss-Fels KP, Robinson H, Mudge SR, Richard C, Newman S, Wittkop B, Stahl A, Friedt W, Frisch M, Gabur I, Miller-Cooper A, Campbell BC, Kelly A, Fox G, Christopher J, Christopher M, Chenu K, Franckowiak J, Mace ES, Borrell AK, Eagles H, Jordan DR, Botella JR, Hammer G, Godwin ID, Trevaskis B, Snowdon RJ, Hickey LT.

Mol Plant. 2018 Jan 8;11(1):226-229. doi: 10.1016/j.molp.2017.10.005. Epub 2017 Oct 19. No abstract available.


Genome Editing in Cotton with the CRISPR/Cas9 System.

Gao W, Long L, Tian X, Xu F, Liu J, Singh PK, Botella JR, Song C.

Front Plant Sci. 2017 Aug 3;8:1364. doi: 10.3389/fpls.2017.01364. eCollection 2017.


Host Delivered RNAi, an efficient approach to increase rice resistance to sheath blight pathogen (Rhizoctonia solani).

Tiwari IM, Jesuraj A, Kamboj R, Devanna BN, Botella JR, Sharma TR.

Sci Rep. 2017 Aug 8;7(1):7521. doi: 10.1038/s41598-017-07749-w.


Whole-Genome Analysis of Candidate genes Associated with Seed Size and Weight in Sorghum bicolor Reveals Signatures of Artificial Selection and Insights into Parallel Domestication in Cereal Crops.

Tao Y, Mace ES, Tai S, Cruickshank A, Campbell BC, Zhao X, Van Oosterom EJ, Godwin ID, Botella JR, Jordan DR.

Front Plant Sci. 2017 Jul 18;8:1237. doi: 10.3389/fpls.2017.01237. eCollection 2017.


Novel Chitinase Gene LOC_Os11g47510 from Indica Rice Tetep Provides Enhanced Resistance against Sheath Blight Pathogen Rhizoctonia solani in Rice.

Richa K, Tiwari IM, Devanna BN, Botella JR, Sharma V, Sharma TR.

Front Plant Sci. 2017 Apr 25;8:596. doi: 10.3389/fpls.2017.00596. eCollection 2017.


Short tandem target mimic rice lines uncover functions of miRNAs in regulating important agronomic traits.

Zhang H, Zhang J, Yan J, Gou F, Mao Y, Tang G, Botella JR, Zhu JK.

Proc Natl Acad Sci U S A. 2017 May 16;114(20):5277-5282. doi: 10.1073/pnas.1703752114. Epub 2017 May 1.


Gene Targeting by Homology-Directed Repair in Rice Using a Geminivirus-Based CRISPR/Cas9 System.

Wang M, Lu Y, Botella JR, Mao Y, Hua K, Zhu JK.

Mol Plant. 2017 Jul 5;10(7):1007-1010. doi: 10.1016/j.molp.2017.03.002. Epub 2017 Mar 16. No abstract available.


Specific and Sensitive Isothermal Electrochemical Biosensor for Plant Pathogen DNA Detection with Colloidal Gold Nanoparticles as Probes.

Lau HY, Wu H, Wee EJ, Trau M, Wang Y, Botella JR.

Sci Rep. 2017 Jan 17;7:38896. doi: 10.1038/srep38896.


Whole Genome Sequencing Reveals Potential New Targets for Improving Nitrogen Uptake and Utilization in Sorghum bicolor.

Massel K, Campbell BC, Mace ES, Tai S, Tao Y, Worland BG, Jordan DR, Botella JR, Godwin ID.

Front Plant Sci. 2016 Oct 25;7:1544. eCollection 2016.


Heritability of targeted gene modifications induced by plant-optimized CRISPR systems.

Mao Y, Botella JR, Zhu JK.

Cell Mol Life Sci. 2017 Mar;74(6):1075-1093. doi: 10.1007/s00018-016-2380-1. Epub 2016 Sep 27. Review.


Saltational evolution of the heterotrimeric G protein signaling mechanisms in the plant kingdom.

Urano D, Maruta N, Trusov Y, Stoian R, Wu Q, Liang Y, Jaiswal DK, Thung L, Jackson D, Botella JR, Jones AM.

Sci Signal. 2016 Sep 20;9(446):ra93. doi: 10.1126/scisignal.aaf9558.


Heterotrimeric G-proteins facilitate resistance to plant pathogenic viruses in Arabidopsis thaliana (L.) Heynh.

Brenya E, Trusov Y, Dietzgen RG, Botella JR.

Plant Signal Behav. 2016 Aug 2;11(8):e1212798. doi: 10.1080/15592324.2016.1212798.


Field Demonstration of a Multiplexed Point-of-Care Diagnostic Platform for Plant Pathogens.

Lau HY, Wang Y, Wee EJ, Botella JR, Trau M.

Anal Chem. 2016 Aug 16;88(16):8074-81. doi: 10.1021/acs.analchem.6b01551. Epub 2016 Jul 27.


Plant G-Proteins Come of Age: Breaking the Bond with Animal Models.

Trusov Y, Botella JR.

Front Chem. 2016 May 24;4:24. doi: 10.3389/fchem.2016.00024. eCollection 2016.


Functional Characterization of Novel Chitinase Genes Present in the Sheath Blight Resistance QTL: qSBR11-1 in Rice Line Tetep.

Richa K, Tiwari IM, Kumari M, Devanna BN, Sonah H, Kumari A, Nagar R, Sharma V, Botella JR, Sharma TR.

Front Plant Sci. 2016 Mar 1;7:244. doi: 10.3389/fpls.2016.00244. eCollection 2016.



Botella JR, Botella MA.

Methods Mol Biol. 2016;1363:vii-x. No abstract available.


Type B Heterotrimeric G Protein γ-Subunit Regulates Auxin and ABA Signaling in Tomato.

Subramaniam G, Trusov Y, Lopez-Encina C, Hayashi S, Batley J, Botella JR.

Plant Physiol. 2016 Feb;170(2):1117-34. doi: 10.1104/pp.15.01675. Epub 2015 Dec 14.


A multiplex CRISPR/Cas9 platform for fast and efficient editing of multiple genes in Arabidopsis.

Zhang Z, Mao Y, Ha S, Liu W, Botella JR, Zhu JK.

Plant Cell Rep. 2016 Jul;35(7):1519-33. doi: 10.1007/s00299-015-1900-z. Epub 2015 Dec 10.


Simplified Assays for Evaluation of Resistance to Alternaria brassicicola and Turnip Mosaic Virus.

Trusov Y, Dietzgen RG, Maruta N, Botella JR.

Methods Mol Biol. 2016;1363:219-28. doi: 10.1007/978-1-4939-3115-6_18.


Yeast Three-Hybrid System for the Detection of Protein-Protein Interactions.

Maruta N, Trusov Y, Botella JR.

Methods Mol Biol. 2016;1363:145-54. doi: 10.1007/978-1-4939-3115-6_12.


Heterotrimeric G proteins interact with defense-related receptor-like kinases in Arabidopsis.

Aranda-Sicilia MN, Trusov Y, Maruta N, Chakravorty D, Zhang Y, Botella JR.

J Plant Physiol. 2015 Sep 1;188:44-8. doi: 10.1016/j.jplph.2015.09.005. Epub 2015 Sep 24.


Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications in Arabidopsis.

Mao Y, Zhang Z, Feng Z, Wei P, Zhang H, Botella JR, Zhu JK.

Plant Biotechnol J. 2016 Feb;14(2):519-32. doi: 10.1111/pbi.12468. Epub 2015 Sep 11.


TALEN-mediated targeted mutagenesis produces a large variety of heritable mutations in rice.

Zhang H, Gou F, Zhang J, Liu W, Li Q, Mao Y, Botella JR, Zhu JK.

Plant Biotechnol J. 2016 Jan;14(1):186-94. doi: 10.1111/pbi.12372. Epub 2015 Apr 13.


Down-regulation of Fusarium oxysporum endogenous genes by Host-Delivered RNA interference enhances disease resistance.

Hu Z, Parekh U, Maruta N, Trusov Y, Botella JR.

Front Chem. 2015 Jan 20;3:1. doi: 10.3389/fchem.2015.00001. eCollection 2015.


Re-purposing bridging flocculation for on-site, rapid, qualitative DNA detection in resource-poor settings.

Wee EJ, Lau HY, Botella JR, Trau M.

Chem Commun (Camb). 2015 Apr 7;51(27):5828-31. doi: 10.1039/c4cc10068a. Epub 2015 Jan 26.


Evidence for an unusual transmembrane configuration of AGG3, a class C Gγ subunit of Arabidopsis.

Wolfenstetter S, Chakravorty D, Kula R, Urano D, Trusov Y, Sheahan MB, McCurdy DW, Assmann SM, Jones AM, Botella JR.

Plant J. 2015 Feb;81(3):388-98. doi: 10.1111/tpj.12732. Epub 2014 Dec 22.


Molecular inversion probe: a new tool for highly specific detection of plant pathogens.

Lau HY, Palanisamy R, Trau M, Botella JR.

PLoS One. 2014 Oct 24;9(10):e111182. doi: 10.1371/journal.pone.0111182. eCollection 2014.


The pineapple AcMADS1 promoter confers high level expression in tomato and Arabidopsis flowering and fruiting tissues, but AcMADS1 does not complement the tomato LeMADS-RIN (rin) mutant.

Moyle RL, Koia JH, Vrebalov J, Giovannoni J, Botella JR.

Plant Mol Biol. 2014 Nov;86(4-5):395-407. doi: 10.1007/s11103-014-0236-3. Epub 2014 Aug 20.


Fusarium oxysporum infection assays in Arabidopsis.

Trusov Y, Chakravorty D, Botella JR.

Methods Mol Biol. 2013;1043:67-72. doi: 10.1007/978-1-62703-532-3_7.


Heterotrimeric G protein signalling in the plant kingdom.

Urano D, Chen JG, Botella JR, Jones AM.

Open Biol. 2013 Mar 27;3(3):120186. doi: 10.1098/rsob.120186. Review.


Signaling specificity provided by the Arabidopsis thaliana heterotrimeric G-protein γ subunits AGG1 and AGG2 is partially but not exclusively provided through transcriptional regulation.

Thung L, Chakravorty D, Trusov Y, Jones AM, Botella JR.

PLoS One. 2013;8(3):e58503. doi: 10.1371/journal.pone.0058503. Epub 2013 Mar 8.


Heterotrimeric G proteins serve as a converging point in plant defense signaling activated by multiple receptor-like kinases.

Liu J, Ding P, Sun T, Nitta Y, Dong O, Huang X, Yang W, Li X, Botella JR, Zhang Y.

Plant Physiol. 2013 Apr;161(4):2146-58. doi: 10.1104/pp.112.212431. Epub 2013 Feb 19.


Pineapple translation factor SUI1 and ribosomal protein L36 promoters drive constitutive transgene expression patterns in Arabidopsis thaliana.

Koia J, Moyle R, Hendry C, Lim L, Botella JR.

Plant Mol Biol. 2013 Mar;81(4-5):327-36. doi: 10.1007/s11103-012-0002-3. Epub 2012 Dec 22.


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