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Moderate positive predictive value of a multiplex real-time PCR on whole blood for pathogen detection in critically ill patients with sepsis.

van de Groep K, Bos MP, Varkila MRJ, Savelkoul PHM, Ong DSY, Derde LPG, Juffermans NP, van der Poll T, Bonten MJM, Cremer OL; MARS consortium.

Eur J Clin Microbiol Infect Dis. 2019 Oct;38(10):1829-1836. doi: 10.1007/s10096-019-03616-w. Epub 2019 Jun 26.


Development and first evaluation of a novel multiplex real-time PCR on whole blood samples for rapid pathogen identification in critically ill patients with sepsis.

van de Groep K, Bos MP, Savelkoul PHM, Rubenjan A, Gazenbeek C, Melchers WJG, van der Poll T, Juffermans NP, Ong DSY, Bonten MJM, Cremer OL; MARS consortium.

Eur J Clin Microbiol Infect Dis. 2018 Jul;37(7):1333-1344. doi: 10.1007/s10096-018-3255-1. Epub 2018 Apr 26.


Evaluation of a real-time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates with suspected late-onset sepsis.

van den Brand M, van den Dungen FAM, Bos MP, van Weissenbruch MM, van Furth AM, de Lange A, Rubenjan A, Peters RPH, Savelkoul PHM.

Crit Care. 2018 Apr 22;22(1):105. doi: 10.1186/s13054-018-2010-4.


Porous Silicon-Based Biosensors: Towards Real-Time Optical Detection of Target Bacteria in the Food Industry.

Massad-Ivanir N, Shtenberg G, Raz N, Gazenbeek C, Budding D, Bos MP, Segal E.

Sci Rep. 2016 Nov 30;6:38099. doi: 10.1038/srep38099.


A novel quantitative PCR assay for the detection of Streptococcus pneumoniae using the competence regulator gene target comX.

Habets MN, Cremers AJ, Bos MP, Savelkoul P, Eleveld MJ, Meis JF, Hermans PW, Melchers WJ, de Jonge MI, Diavatopoulos DA.

J Med Microbiol. 2016 Feb;65(2):129-36. doi: 10.1099/jmm.0.000204. Epub 2015 Dec 1.


Experimental Methods for Studying the BAM Complex in Neisseria meningitidis.

Bos MP, Boxtel RT, Tommassen J.

Methods Mol Biol. 2015;1329:33-49. doi: 10.1007/978-1-4939-2871-2_3.


Transport of lipopolysaccharide to the Gram-negative bacterial cell surface.

Putker F, Bos MP, Tommassen J.

FEMS Microbiol Rev. 2015 Nov;39(6):985-1002. doi: 10.1093/femsre/fuv026. Epub 2015 Jun 1. Review.


Development of a multiplex real-time PCR assay for the rapid diagnosis of neonatal late onset sepsis.

van den Brand M, Peters RPH, Catsburg A, Rubenjan A, Broeke FJ, van den Dungen FAM, van Weissenbruch MM, van Furth AM, Kõressaar T, Remm M, Savelkoul PHM, Bos MP.

J Microbiol Methods. 2014 Nov;106:8-15. doi: 10.1016/j.mimet.2014.07.034. Epub 2014 Aug 4.


Involvement of Neisseria meningitidis lipoprotein GNA2091 in the assembly of a subset of outer membrane proteins.

Bos MP, Grijpstra J, Tommassen-van Boxtel R, Tommassen J.

J Biol Chem. 2014 May 30;289(22):15602-10. doi: 10.1074/jbc.M113.539510. Epub 2014 Apr 22.


Species-specificity of the BamA component of the bacterial outer membrane protein-assembly machinery.

Volokhina EB, Grijpstra J, Beckers F, Lindh E, Robert V, Tommassen J, Bos MP.

PLoS One. 2013 Dec 20;8(12):e85799. doi: 10.1371/journal.pone.0085799. eCollection 2013.


Ght protein of Neisseria meningitidis is involved in the regulation of lipopolysaccharide biosynthesis.

Putker F, Grutsch A, Tommassen J, Bos MP.

J Bacteriol. 2014 Feb;196(4):780-9. doi: 10.1128/JB.00943-13. Epub 2013 Dec 2.


Zinc piracy as a mechanism of Neisseria meningitidis for evasion of nutritional immunity.

Stork M, Grijpstra J, Bos MP, Mañas Torres C, Devos N, Poolman JT, Chazin WJ, Tommassen J.

PLoS Pathog. 2013 Oct;9(10):e1003733. doi: 10.1371/journal.ppat.1003733. Epub 2013 Oct 31.


Comparison of pathogen DNA isolation methods from large volumes of whole blood to improve molecular diagnosis of bloodstream infections.

Loonen AJ, Bos MP, van Meerbergen B, Neerken S, Catsburg A, Dobbelaer I, Penterman R, Maertens G, van de Wiel P, Savelkoul P, van den Brule AJ.

PLoS One. 2013 Aug 15;8(8):e72349. doi: 10.1371/journal.pone.0072349. eCollection 2013.


Assembly of bacterial outer membrane proteins.

Grijpstra J, Bos MP, Tommassen J.

Methods Mol Biol. 2013;966:223-37. doi: 10.1007/978-1-62703-245-2_14.


Cellular solid-state nuclear magnetic resonance spectroscopy.

Renault M, Tommassen-van Boxtel R, Bos MP, Post JA, Tommassen J, Baldus M.

Proc Natl Acad Sci U S A. 2012 Mar 27;109(13):4863-8. doi: 10.1073/pnas.1116478109. Epub 2012 Feb 13.


Solid-state NMR spectroscopy on cellular preparations enhanced by dynamic nuclear polarization.

Renault M, Pawsey S, Bos MP, Koers EJ, Nand D, Tommassen-van Boxtel R, Rosay M, Tommassen J, Maas WE, Baldus M.

Angew Chem Int Ed Engl. 2012 Mar 19;51(12):2998-3001. doi: 10.1002/anie.201105984. Epub 2012 Feb 1. No abstract available.


Generating knock-out and complementation strains of Neisseria meningitidis.

van Dam V, Bos MP.

Methods Mol Biol. 2012;799:55-72. doi: 10.1007/978-1-61779-346-2_4.


The LptD chaperone LptE is not directly involved in lipopolysaccharide transport in Neisseria meningitidis.

Bos MP, Tommassen J.

J Biol Chem. 2011 Aug 19;286(33):28688-96. doi: 10.1074/jbc.M111.239673. Epub 2011 Jun 24.


Solid-state NMR on a large multidomain integral membrane protein: the outer membrane protein assembly factor BamA.

Renault M, Bos MP, Tommassen J, Baldus M.

J Am Chem Soc. 2011 Mar 30;133(12):4175-7. doi: 10.1021/ja109469c. Epub 2011 Mar 1.


Structural characterization of outer membrane components of the type IV pili system in pathogenic Neisseria.

Jain S, Mościcka KB, Bos MP, Pachulec E, Stuart MC, Keegstra W, Boekema EJ, van der Does C.

PLoS One. 2011 Jan 31;6(1):e16624. doi: 10.1371/journal.pone.0016624.


Role of the periplasmic chaperones Skp, SurA, and DegQ in outer membrane protein biogenesis in Neisseria meningitidis.

Volokhina EB, Grijpstra J, Stork M, Schilders I, Tommassen J, Bos MP.

J Bacteriol. 2011 Apr;193(7):1612-21. doi: 10.1128/JB.00532-10. Epub 2011 Feb 4.


An outer membrane receptor of Neisseria meningitidis involved in zinc acquisition with vaccine potential.

Stork M, Bos MP, Jongerius I, de Kok N, Schilders I, Weynants VE, Poolman JT, Tommassen J.

PLoS Pathog. 2010 Jul 1;6:e1000969. doi: 10.1371/journal.ppat.1000969.


NalP-mediated proteolytic release of lactoferrin-binding protein B from the meningococcal cell surface.

Roussel-Jazédé V, Jongerius I, Bos MP, Tommassen J, van Ulsen P.

Infect Immun. 2010 Jul;78(7):3083-9. doi: 10.1128/IAI.01193-09. Epub 2010 Apr 26.


The mitochondrial porin, VDAC, has retained the ability to be assembled in the bacterial outer membrane.

Walther DM, Bos MP, Rapaport D, Tommassen J.

Mol Biol Evol. 2010 Apr;27(4):887-95. doi: 10.1093/molbev/msp294. Epub 2009 Dec 3.


The beta-barrel outer membrane protein assembly complex of Neisseria meningitidis.

Volokhina EB, Beckers F, Tommassen J, Bos MP.

J Bacteriol. 2009 Nov;191(22):7074-85. doi: 10.1128/JB.00737-09. Epub 2009 Sep 18.


Signals in bacterial beta-barrel proteins are functional in eukaryotic cells for targeting to and assembly in mitochondria.

Walther DM, Papic D, Bos MP, Tommassen J, Rapaport D.

Proc Natl Acad Sci U S A. 2009 Feb 24;106(8):2531-6. doi: 10.1073/pnas.0807830106. Epub 2009 Jan 30.


Functioning of outer membrane protein assembly factor Omp85 requires a single POTRA domain.

Bos MP, Robert V, Tommassen J.

EMBO Rep. 2007 Dec;8(12):1149-54. Epub 2007 Nov 9.


Additive and synergistic bactericidal activity of antibodies directed against minor outer membrane proteins of Neisseria meningitidis.

Weynants VE, Feron CM, Goraj KK, Bos MP, Denoël PA, Verlant VG, Tommassen J, Peak IR, Judd RC, Jennings MP, Poolman JT.

Infect Immun. 2007 Nov;75(11):5434-42. Epub 2007 Jul 30.


Biogenesis of the gram-negative bacterial outer membrane.

Bos MP, Robert V, Tommassen J.

Annu Rev Microbiol. 2007;61:191-214. Review.


Assembly factor Omp85 recognizes its outer membrane protein substrates by a species-specific C-terminal motif.

Robert V, Volokhina EB, Senf F, Bos MP, Van Gelder P, Tommassen J.

PLoS Biol. 2006 Nov;4(11):e377.


Shielding of immunogenic domains in Neisseria meningitidis FrpB (FetA) by the major variable region.

Kortekaas J, Pettersson A, van der Biezen J, Weynants VE, van der Ley P, Poolman J, Bos MP, Tommassen J.

Vaccine. 2007 Jan 2;25(1):72-84. Epub 2006 Jul 28.


Immunogenicity and structural characterisation of an in vitro folded meningococcal siderophore receptor (FrpB, FetA).

Kortekaas J, Müller SA, Ringler P, Gregorini M, Weynants VE, Rutten L, Bos MP, Tommassen J.

Microbes Infect. 2006 Jul;8(8):2145-53. Epub 2006 May 24.


Vaccine potential of the Neisseria meningitidis lactoferrin-binding proteins LbpA and LbpB.

Pettersson A, Kortekaas J, Weynants VE, Voet P, Poolman JT, Bos MP, Tommassen J.

Vaccine. 2006 Apr 24;24(17):3545-57. Epub 2006 Feb 20.


MsbA is not required for phospholipid transport in Neisseria meningitidis.

Tefsen B, Bos MP, Beckers F, Tommassen J, de Cock H.

J Biol Chem. 2005 Oct 28;280(43):35961-6. Epub 2005 Aug 25.


Function of neisserial outer membrane phospholipase a in autolysis and assessment of its vaccine potential.

Bos MP, Tefsen B, Voet P, Weynants V, van Putten JP, Tommassen J.

Infect Immun. 2005 Apr;73(4):2222-31.


Biogenesis of the Gram-negative bacterial outer membrane.

Bos MP, Tommassen J.

Curr Opin Microbiol. 2004 Dec;7(6):610-6. Review.


Identification of an outer membrane protein required for the transport of lipopolysaccharide to the bacterial cell surface.

Bos MP, Tefsen B, Geurtsen J, Tommassen J.

Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9417-22. Epub 2004 Jun 10.


Crystal structure of Neisserial surface protein A (NspA), a conserved outer membrane protein with vaccine potential.

Vandeputte-Rutten L, Bos MP, Tommassen J, Gros P.

J Biol Chem. 2003 Jul 4;278(27):24825-30. Epub 2003 Apr 26.


Role of a highly conserved bacterial protein in outer membrane protein assembly.

Voulhoux R, Bos MP, Geurtsen J, Mols M, Tommassen J.

Science. 2003 Jan 10;299(5604):262-5.


Conformational analysis of opacity proteins from Neisseria meningitidis.

de Jonge MI, Bos MP, Hamstra HJ, Jiskoot W, van Ulsen P, Tommassen J, van Alphen L, van der Ley P.

Eur J Biochem. 2002 Nov;269(21):5215-23.


CD66 receptor specificity exhibited by neisserial Opa variants is controlled by protein determinants in CD66 N-domains.

Bos MP, Kuroki M, Krop-Watorek A, Hogan D, Belland RJ.

Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9584-9.


Selection of Opa+ Neisseria gonorrhoeae by limited availability of normal human serum.

Bos MP, Hogan D, Belland RJ.

Infect Immun. 1997 Feb;65(2):645-50.


Down-regulation of the receptor for parathyroid hormone (PTH) and PTH-related peptide by PTH in primary fetal rat osteoblasts.

Jongen JW, Willemstein-van Hove EC, van der Meer JM, Bos MP, Jüppner H, Segre GV, Abou-Samra AB, Feyen JH, Herrmann-Erlee MP.

J Bone Miner Res. 1996 Sep;11(9):1218-25.


Expression of the parathyroid hormone receptor and correlation with other osteoblastic parameters in fetal rat osteoblasts.

Bos MP, van der Meer JM, Feyen JH, Herrmann-Erlee MP.

Calcif Tissue Int. 1996 Feb;58(2):95-100.


Down-regulation and differential restoration of cAMP responses upon transient phorbol ester treatment of primary osteoblastic cells.

Bos MP, Van der Meer JM, Feyen JH, Herrmann-Erlee MP.

Cell Signal. 1995 Aug;7(6):617-26.


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