Format

Send to

Choose Destination
Angew Chem Int Ed Engl. 2018 Dec 21;57(52):17028-17032. doi: 10.1002/anie.201810331. Epub 2018 Nov 20.

Self-Assembling All-Enzyme Hydrogels for Flow Biocatalysis.

Author information

1
Karlsruhe Institute of Technology (KIT), Institute for Biological Interfaces (IBG 1), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
2
Karlsruhe Institute of Technology (KIT), Institute for Mechanical Process Engineering and Mechanics, Gotthard-Franz-Strasse 3, 76131, Karlsruhe, Germany.

Abstract

Continuous flow biocatalysis is an emerging field of industrial biotechnology that uses enzymes immobilized in flow channels for the production of value-added chemicals. We describe the construction of self-assembling all-enzyme hydrogels that are comprised of two tetrameric enzymes. The stereoselective dehydrogenase LbADH and the cofactor-regenerating glucose 1-dehydrogenase GDH were genetically fused with a SpyTag or SpyCatcher domain, respectively, to generate two complementary homo-tetrameric building blocks that polymerize under physiological conditions into porous hydrogels. Mounted in microfluidic reactors, the gels show excellent stereoselectivity with near quantitative conversion in the reduction of prochiral ketones along with high robustness under process and storage conditions. The gels function as compartment that retains intermediates thus enabling high total turnover numbers of the expensive cofactor NADP(H).

KEYWORDS:

enzymes; flow biocatalysis; immobilization; microreactors; stereoselective reactions

PMID:
30380178
DOI:
10.1002/anie.201810331

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center