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Plant Cell. 2017 Jun;29(6):1460-1479. doi: 10.1105/tpc.17.00136. Epub 2017 May 30.

Interplay of Plasma Membrane and Vacuolar Ion Channels, Together with BAK1, Elicits Rapid Cytosolic Calcium Elevations in Arabidopsis during Aphid Feeding.

Author information

1
Department of Metabolic Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom.
2
Department of Crop Genetics, John Innes Centre, Norwich NR4 7UH, United Kingdom.
3
Department of Botany, University of Wisconsin, Madison, Wisconsin 53706.
4
Department of Biochemistry and Molecular Biology, Saitama University, Sakura-ku, Saitama 338-8570, Japan.
5
Japan Science and Technology Agency, Precursory Research for Embryonic Science and Technology (PRESTO), Kawaguchi, Saitama 332-0012, Japan.
6
Department of Crop Genetics, John Innes Centre, Norwich NR4 7UH, United Kingdom saskia.hogenhout@jic.ac.uk dale.sanders@jic.ac.uk.
7
Department of Metabolic Biology, John Innes Centre, Norwich NR4 7UH, United Kingdom saskia.hogenhout@jic.ac.uk dale.sanders@jic.ac.uk.

Abstract

A transient rise in cytosolic calcium ion concentration is one of the main signals used by plants in perception of their environment. The role of calcium in the detection of abiotic stress is well documented; however, its role during biotic interactions remains unclear. Here, we use a fluorescent calcium biosensor (GCaMP3) in combination with the green peach aphid (Myzus persicae) as a tool to study Arabidopsis thaliana calcium dynamics in vivo and in real time during a live biotic interaction. We demonstrate rapid and highly localized plant calcium elevations around the feeding sites of M. persicae, and by monitoring aphid feeding behavior electrophysiologically, we demonstrate that these elevations correlate with aphid probing of epidermal and mesophyll cells. Furthermore, we dissect the molecular mechanisms involved, showing that interplay between the plant defense coreceptor BRASSINOSTEROID INSENSITIVE-ASSOCIATED KINASE1 (BAK1), the plasma membrane ion channels GLUTAMATE RECEPTOR-LIKE 3.3 and 3.6 (GLR3.3 and GLR3.6), and the vacuolar ion channel TWO-PORE CHANNEL1 (TPC1) mediate these calcium elevations. Consequently, we identify a link between plant perception of biotic threats by BAK1, cellular calcium entry mediated by GLRs, and intracellular calcium release by TPC1 during a biologically relevant interaction.

PMID:
28559475
PMCID:
PMC5502460
DOI:
10.1105/tpc.17.00136
[Indexed for MEDLINE]
Free PMC Article

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