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Items: 8

1.

Chemically Inducible Chromosomal Evolution (CIChE) for Multicopy Metabolic Pathway Engineering.

Love AM, Biggs BW, Tyo KEJ, Ajikumar PK.

Methods Mol Biol. 2019;1927:37-45. doi: 10.1007/978-1-4939-9142-6_4.

PMID:
30788784
2.

Publisher Correction: Engineered bidirectional promoters enable rapid multi-gene co-expression optimization.

Vogl T, Kickenweiz T, Pitzer J, Sturmberger L, Weninger A, Biggs BW, Köhler EM, Baumschlager A, Fischer JE, Hyden P, Wagner M, Baumann M, Borth N, Geier M, Ajikumar PK, Glieder A.

Nat Commun. 2018 Oct 29;9(1):4566. doi: 10.1038/s41467-018-07112-1.

3.

Engineered bidirectional promoters enable rapid multi-gene co-expression optimization.

Vogl T, Kickenweiz T, Pitzer J, Sturmberger L, Weninger A, Biggs BW, Köhler EM, Baumschlager A, Fischer JE, Hyden P, Wagner M, Baumann M, Borth N, Geier M, Ajikumar PK, Glieder A.

Nat Commun. 2018 Sep 4;9(1):3589. doi: 10.1038/s41467-018-05915-w. Erratum in: Nat Commun. 2018 Oct 29;9(1):4566.

4.

Heterologous expression and characterization of plant Taxadiene-5α-Hydroxylase (CYP725A4) in Escherichia coli.

Rouck JE, Biggs BW, Kambalyal A, Arnold WR, De Mey M, Ajikumar PK, Das A.

Protein Expr Purif. 2017 Apr;132:60-67. doi: 10.1016/j.pep.2017.01.008. Epub 2017 Jan 18.

5.

Overcoming heterologous protein interdependency to optimize P450-mediated Taxol precursor synthesis in Escherichia coli.

Biggs BW, Lim CG, Sagliani K, Shankar S, Stephanopoulos G, De Mey M, Ajikumar PK.

Proc Natl Acad Sci U S A. 2016 Mar 22;113(12):3209-14. doi: 10.1073/pnas.1515826113. Epub 2016 Mar 7.

6.

Orthogonal Assays Clarify the Oxidative Biochemistry of Taxol P450 CYP725A4.

Biggs BW, Rouck JE, Kambalyal A, Arnold W, Lim CG, De Mey M, O'Neil-Johnson M, Starks CM, Das A, Ajikumar PK.

ACS Chem Biol. 2016 May 20;11(5):1445-51. doi: 10.1021/acschembio.5b00968. Epub 2016 Mar 16.

PMID:
26930136
7.

Multivariate modular metabolic engineering for pathway and strain optimization.

Biggs BW, De Paepe B, Santos CN, De Mey M, Kumaran Ajikumar P.

Curr Opin Biotechnol. 2014 Oct;29:156-62. doi: 10.1016/j.copbio.2014.05.005. Epub 2014 Jun 11. Review.

PMID:
24927371
8.

Selective patterning of Si-based biosensor surfaces using isotropic silicon etchants.

Biggs BW, Hunt HK, Armani AM.

J Colloid Interface Sci. 2012 Mar 1;369(1):477-81. doi: 10.1016/j.jcis.2011.11.082. Epub 2011 Dec 11.

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