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Items: 1 to 50 of 348

1.

The human adrenal gland as a drug metabolizer: First in-vivo evidence for the conversion of steroidal drugs.

Hartmann MF, Reincke M, Wudy SA, Bernhardt R.

J Steroid Biochem Mol Biol. 2019 Nov;194:105438. doi: 10.1016/j.jsbmb.2019.105438. Epub 2019 Jul 27.

PMID:
31362063
2.

HIV Drug Efavirenz Inhibits CYP21A2 Activity with Possible Clinical Implications.

Malikova J, Zingg T, Fingerhut R, Sluka S, Grössl M, Brixius-Anderko S, Bernhardt R, McDougall J, Pandey AV, Flück CE.

Horm Res Paediatr. 2019;91(4):262-270. doi: 10.1159/000500522. Epub 2019 Jun 28.

PMID:
31256164
3.

High-yield C11-oxidation of hydrocortisone by establishment of an efficient whole-cell system in Bacillus megaterium.

König L, Hartz P, Bernhardt R, Hannemann F.

Metab Eng. 2019 Sep;55:59-67. doi: 10.1016/j.ymben.2019.06.005. Epub 2019 Jun 15.

PMID:
31212031
4.

Bioinspired calcium phosphate mineralization on Net-Shape-Nonwoven chitosan scaffolds stimulates human bone marrow stromal cell differentiation.

Heinemann C, Brünler R, Kreschel C, Kruppke B, Bernhardt R, Aibibu D, Cherif C, Wiesmann HP, Hanke T.

Biomed Mater. 2019 Jun 19;14(4):045017. doi: 10.1088/1748-605X/ab27a4.

PMID:
31170696
5.

A novel short chain dehydrogenase from Bacillus megaterium for the conversion of the sesquiterpene nootkatol to (+)-nootkatone.

Milhim M, Hartz P, Gerber A, Bernhardt R.

J Biotechnol. 2019 Aug 10;301:52-55. doi: 10.1016/j.jbiotec.2019.05.017. Epub 2019 May 28.

PMID:
31150680
6.

Plasma membrane localization of CYP4Z1 and CYP19A1 and the detection of anti-CYP19A1 autoantibodies in humans.

Khayeka-Wandabwa C, Ma X, Cao X, Nunna V, Pathak JL, Bernhardt R, Cai P, Bureik M.

Int Immunopharmacol. 2019 Aug;73:64-71. doi: 10.1016/j.intimp.2019.05.003. Epub 2019 May 10.

PMID:
31082724
7.

Expanding the promoter toolbox of Bacillus megaterium.

Hartz P, Mattes C, Schad M, Bernhardt R, Hannemann F.

J Biotechnol. 2019 Mar 20;294:38-48. doi: 10.1016/j.jbiotec.2019.01.018. Epub 2019 Feb 13.

PMID:
30771444
8.

Synergistic effect of bimodal pore distribution and artificial extracellular matrices in polymeric scaffolds on osteogenic differentiation of human mesenchymal stem cells.

Wojak-Ćwik IM, Rumian Ł, Krok-Borkowicz M, Hess R, Bernhardt R, Dobrzyński P, Möller S, Schnabelrauch M, Hintze V, Scharnweber D, Pamuła E.

Mater Sci Eng C Mater Biol Appl. 2019 Apr;97:12-22. doi: 10.1016/j.msec.2018.12.012. Epub 2018 Dec 7.

PMID:
30678897
9.

Novel approach to improve progesterone hydroxylation selectivity by CYP106A2 via rational design of adrenodoxin binding.

Sagadin T, Riehm J, Putkaradze N, Hutter MC, Bernhardt R.

FEBS J. 2019 Mar;286(6):1240-1249. doi: 10.1111/febs.14722. Epub 2019 Jan 2.

PMID:
30537187
10.

Sesquiterpenoids Produced by Combining Two Sesquiterpene Cyclases with Promiscuous Myxobacterial CYP260B1.

Yuan Y, Litzenburger M, Cheng S, Bian G, Hu B, Yan P, Cai Y, Deng Z, Bernhardt R, Liu T.

Chembiochem. 2019 Mar 1;20(5):677-682. doi: 10.1002/cbic.201800670. Epub 2019 Jan 25.

PMID:
30484946
11.

CYP109E1 from Bacillus megaterium Acts as a 24- and 25-Hydroxylase for Cholesterol.

Putkaradze N, Litzenburger M, Hutter MC, Bernhardt R.

Chembiochem. 2019 Mar 1;20(5):655-658. doi: 10.1002/cbic.201800595. Epub 2019 Jan 9.

PMID:
30398298
12.

Binding modes of CYP106A2 redox partners determine differences in progesterone hydroxylation product patterns.

Sagadin T, Riehm JL, Milhim M, Hutter MC, Bernhardt R.

Commun Biol. 2018 Jul 30;1:99. doi: 10.1038/s42003-018-0104-9. eCollection 2018.

13.

Correction to: Bacterial steroid hydroxylases: enzyme classes, their functions and comparison of their catalytic mechanisms.

Szaleniec M, Wojtkiewicz AM, Bernhardt R, Borowski T, Donova M.

Appl Microbiol Biotechnol. 2018 Oct;102(19):8173. doi: 10.1007/s00253-018-9320-y.

14.

Characterization and engineering of a carotenoid biosynthesis operon from Bacillus megaterium.

Hartz P, Milhim M, Trenkamp S, Bernhardt R, Hannemann F.

Metab Eng. 2018 Sep;49:47-58. doi: 10.1016/j.ymben.2018.07.017. Epub 2018 Jul 26.

PMID:
30055324
15.

Structural insights into oxidation of medium-chain fatty acids and flavanone by myxobacterial cytochrome P450 CYP267B1.

Jóźwik IK, Litzenburger M, Khatri Y, Schifrin A, Girhard M, Urlacher V, Thunnissen AWH, Bernhardt R.

Biochem J. 2018 Sep 11;475(17):2801-2817. doi: 10.1042/BCJ20180402.

PMID:
30045877
16.

Bacterial steroid hydroxylases: enzyme classes, their functions and comparison of their catalytic mechanisms.

Szaleniec M, Wojtkiewicz AM, Bernhardt R, Borowski T, Donova M.

Appl Microbiol Biotechnol. 2018 Oct;102(19):8153-8171. doi: 10.1007/s00253-018-9239-3. Epub 2018 Jul 21. Review. Erratum in: Appl Microbiol Biotechnol. 2018 Aug 23;:.

17.

An Isotopic Labelling Strategy to Study Cytochrome P450 Oxidations of Terpenes.

Rinkel J, Litzenburger M, Bernhardt R, Dickschat JS.

Chembiochem. 2018 Apr 26. doi: 10.1002/cbic.201800215. [Epub ahead of print]

PMID:
29697903
18.

Structure-Based Engineering of Steroidogenic CYP260A1 for Stereo- and Regioselective Hydroxylation of Progesterone.

Khatri Y, Jóźwik IK, Ringle M, Ionescu IA, Litzenburger M, Hutter MC, Thunnissen AWH, Bernhardt R.

ACS Chem Biol. 2018 Apr 20;13(4):1021-1028. doi: 10.1021/acschembio.8b00026. Epub 2018 Mar 13.

PMID:
29509407
19.

Influence of estrogen on individual exercise motivation and bone protection in ovariectomized rats.

Müller ST, Keiler AM, Kräker K, Zierau O, Bernhardt R.

Lab Anim. 2018 Oct;52(5):479-489. doi: 10.1177/0023677218756455. Epub 2018 Feb 9.

PMID:
29426272
20.

Efficiency of the sulfate pathway in comparison to the Δ4- and Δ5-pathway of steroidogenesis in the porcine testis.

Klymiuk MC, Neunzig J, Bernhardt R, Sánchez-Guijo A, Hartmann MF, Wudy SA, Schuler G.

J Steroid Biochem Mol Biol. 2018 May;179:64-72. doi: 10.1016/j.jsbmb.2017.10.017. Epub 2017 Nov 6.

PMID:
29107177
21.

CYP109E1 is a novel versatile statin and terpene oxidase from Bacillus megaterium.

Putkaradze N, Litzenburger M, Abdulmughni A, Milhim M, Brill E, Hannemann F, Bernhardt R.

Appl Microbiol Biotechnol. 2017 Dec;101(23-24):8379-8393. doi: 10.1007/s00253-017-8552-6. Epub 2017 Oct 11.

PMID:
29018905
22.

Physicochemical properties of sorghum flour are selectively modified by combined germination-fermentation.

Elkhalifa AEO, Bernhardt R, Cardone G, Marti A, Iametti S, Marengo M.

J Food Sci Technol. 2017 Sep;54(10):3307-3313. doi: 10.1007/s13197-017-2781-7. Epub 2017 Aug 22.

23.

Biochemical and structural characterization of CYP109A2, a vitamin D3 25-hydroxylase from Bacillus megaterium.

Abdulmughni A, Jóźwik IK, Brill E, Hannemann F, Thunnissen AWH, Bernhardt R.

FEBS J. 2017 Nov;284(22):3881-3894. doi: 10.1111/febs.14276. Epub 2017 Oct 16.

24.

A standardized Humulus lupulus (L.) ethanol extract partially prevents ovariectomy-induced bone loss in the rat without induction of adverse effects in the uterus.

Keiler AM, Helle J, Bader MI, Ehrhardt T, Nestler K, Kretzschmar G, Bernhardt R, Vollmer G, Nikolić D, Bolton JL, Pauli GF, Chen SN, Dietz BM, van Breemen RB, Zierau O.

Phytomedicine. 2017 Oct 15;34:50-58. doi: 10.1016/j.phymed.2017.08.001. Epub 2017 Aug 2.

25.

CYP17A1 inhibitor abiraterone, an anti-prostate cancer drug, also inhibits the 21-hydroxylase activity of CYP21A2.

Malikova J, Brixius-Anderko S, Udhane SS, Parween S, Dick B, Bernhardt R, Pandey AV.

J Steroid Biochem Mol Biol. 2017 Nov;174:192-200. doi: 10.1016/j.jsbmb.2017.09.007. Epub 2017 Sep 8.

PMID:
28893623
26.

Functionalized poly(3-hydroxybutyric acid) bodies as new in vitro biocatalysts.

Stenger B, Gerber A, Bernhardt R, Hannemann F.

Biochim Biophys Acta Proteins Proteom. 2018 Jan;1866(1):52-59. doi: 10.1016/j.bbapap.2017.08.010. Epub 2017 Sep 15.

PMID:
28870733
27.

Engineering of versatile redox partner fusions that support monooxygenase activity of functionally diverse cytochrome P450s.

Bakkes PJ, Riehm JL, Sagadin T, Rühlmann A, Schubert P, Biemann S, Girhard M, Hutter MC, Bernhardt R, Urlacher VB.

Sci Rep. 2017 Aug 29;7(1):9570. doi: 10.1038/s41598-017-10075-w.

28.

CYP260B1 acts as 9α-hydroxylase for 11-deoxycorticosterone.

Litzenburger M, Bernhardt R.

Steroids. 2017 Nov;127:40-45. doi: 10.1016/j.steroids.2017.08.006. Epub 2017 Aug 18.

PMID:
28827071
29.

CYP106A2-A versatile biocatalyst with high potential for biotechnological production of selectively hydroxylated steroid and terpenoid compounds.

Schmitz D, Janocha S, Kiss FM, Bernhardt R.

Biochim Biophys Acta Proteins Proteom. 2018 Jan;1866(1):11-22. doi: 10.1016/j.bbapap.2017.07.011. Epub 2017 Aug 2. Review.

PMID:
28780179
30.

Effect of sulfonated steroids on steroidogenic cytochrome P450-dependent steroid hydroxylases.

Neunzig J, Bernhardt R.

J Steroid Biochem Mol Biol. 2018 May;179:3-7. doi: 10.1016/j.jsbmb.2017.07.004. Epub 2017 Jul 8. Review.

PMID:
28694196
31.

Raman and infrared spectroscopic evidence for the structural changes of the 2Fe2S cluster and its environment during the interaction of adrenodoxin and adrenodoxin reductase.

Khalil M, Bernhardt R, Hellwig P.

Spectrochim Acta A Mol Biomol Spectrosc. 2017 Aug 5;183:298-305. doi: 10.1016/j.saa.2017.04.064. Epub 2017 Apr 22.

PMID:
28458234
32.

Improvement of a P450-Based Recombinant Escherichia coli Whole-Cell System for the Production of Oxygenated Sesquiterpene Derivatives.

Ly TTB, Schifrin A, Nguyen BD, Bernhardt R.

J Agric Food Chem. 2017 May 17;65(19):3891-3899. doi: 10.1021/acs.jafc.7b00792. Epub 2017 May 4.

PMID:
28447451
33.

Investigating the effect of available redox protein ratios for the conversion of a steroid by a myxobacterial CYP260A1.

Khatri Y, Schifrin A, Bernhardt R.

FEBS Lett. 2017 Apr;591(8):1126-1140. doi: 10.1002/1873-3468.12619. Epub 2017 Apr 6.

34.

The impact of the clinical CYP11B2 mutation V386A strongly depends on the enzyme's genetic background.

Neunzig J, Khatri Y, Bernhardt R.

Endocr J. 2017 Apr 29;64(4):457-461. doi: 10.1507/endocrj.EJ16-0417. Epub 2017 Feb 10.

35.

Engineering of CYP106A2 for steroid 9α- and 6β-hydroxylation.

Nikolaus J, Nguyen KT, Virus C, Riehm JL, Hutter M, Bernhardt R.

Steroids. 2017 Apr;120:41-48. doi: 10.1016/j.steroids.2017.01.005. Epub 2017 Feb 3.

PMID:
28163026
36.

The steroid metabolite 16(β)-OH-androstenedione generated by CYP21A2 serves as a substrate for CYP19A1.

Neunzig J, Milhim M, Schiffer L, Khatri Y, Zapp J, Sánchez-Guijo A, Hartmann MF, Wudy SA, Bernhardt R.

J Steroid Biochem Mol Biol. 2017 Mar;167:182-191. doi: 10.1016/j.jsbmb.2017.01.002. Epub 2017 Jan 5.

PMID:
28065637
37.

Characterization of cytochrome P450 CYP109E1 from Bacillus megaterium as a novel vitamin D3 hydroxylase.

Abdulmughni A, Jóźwik IK, Putkaradze N, Brill E, Zapp J, Thunnissen AW, Hannemann F, Bernhardt R.

J Biotechnol. 2017 Feb 10;243:38-47. doi: 10.1016/j.jbiotec.2016.12.023. Epub 2016 Dec 30.

PMID:
28043840
38.

Biotransformation of prednisone and dexamethasone by cytochrome P450 based systems - Identification of new potential drug candidates.

Putkaradze N, Kiss FM, Schmitz D, Zapp J, Hutter MC, Bernhardt R.

J Biotechnol. 2017 Jan 20;242:101-110. doi: 10.1016/j.jbiotec.2016.12.011. Epub 2016 Dec 14.

PMID:
27988215
39.

Collagen/glycosaminoglycan coatings enhance new bone formation in a critical size bone defect - A pilot study in rats.

Förster Y, Bernhardt R, Hintze V, Möller S, Schnabelrauch M, Scharnweber D, Rammelt S.

Mater Sci Eng C Mater Biol Appl. 2017 Feb 1;71:84-92. doi: 10.1016/j.msec.2016.09.071. Epub 2016 Sep 29.

PMID:
27987780
40.

Investigating the roles of T224 and T232 in the oxidation of cinnamaldehyde catalyzed by myxobacterial CYP260B1.

Litzenburger M, Lo Izzo R, Bernhardt R, Khatri Y.

FEBS Lett. 2017 Jan;591(1):39-46. doi: 10.1002/1873-3468.12519. Epub 2016 Dec 24.

41.

Structural characterization of CYP260A1 from Sorangium cellulosum to investigate the 1α-hydroxylation of a mineralocorticoid.

Khatri Y, Carius Y, Ringle M, Lancaster CR, Bernhardt R.

FEBS Lett. 2016 Dec;590(24):4638-4648. doi: 10.1002/1873-3468.12479. Epub 2016 Nov 23.

42.

Identification of a new plasmid-encoded cytochrome P450 CYP107DY1 from Bacillus megaterium with a catalytic activity towards mevastatin.

Milhim M, Putkaradze N, Abdulmughni A, Kern F, Hartz P, Bernhardt R.

J Biotechnol. 2016 Dec 20;240:68-75. doi: 10.1016/j.jbiotec.2016.11.002. Epub 2016 Nov 2.

PMID:
27816653
43.

Structural basis of steroid binding and oxidation by the cytochrome P450 CYP109E1 from Bacillus megaterium.

Jóźwik IK, Kiss FM, Gricman Ł, Abdulmughni A, Brill E, Zapp J, Pleiss J, Bernhardt R, Thunnissen AW.

FEBS J. 2016 Nov;283(22):4128-4148. doi: 10.1111/febs.13911. Epub 2016 Oct 17.

44.

Scrutiny of electrochemically-driven electrocatalysis of C-19 steroid 1α-hydroxylase (CYP260A1) from Sorangium cellulosum So ce56.

Kuzikov AV, Masamrekh RA, Khatri Y, Zavialova MG, Bernhardt R, Archakov AI, Shumyantseva VV.

Anal Biochem. 2016 Nov 15;513:28-35. doi: 10.1016/j.ab.2016.08.016. Epub 2016 Aug 25.

PMID:
27567992
45.

Role of steroid sulfatase in steroid homeostasis and characterization of the sulfated steroid pathway: Evidence from steroid sulfatase deficiency.

Sánchez-Guijo A, Neunzig J, Gerber A, Oji V, Hartmann MF, Schuppe HC, Traupe H, Bernhardt R, Wudy SA.

Mol Cell Endocrinol. 2016 Dec 5;437:142-153. doi: 10.1016/j.mce.2016.08.019. Epub 2016 Aug 13.

PMID:
27531568
46.

The role of sulfated steroid hormones in reproductive processes.

Geyer J, Bakhaus K, Bernhardt R, Blaschka C, Dezhkam Y, Fietz D, Grosser G, Hartmann K, Hartmann MF, Neunzig J, Papadopoulos D, Sánchez-Guijo A, Scheiner-Bobis G, Schuler G, Shihan M, Wrenzycki C, Wudy SA, Bergmann M.

J Steroid Biochem Mol Biol. 2017 Sep;172:207-221. doi: 10.1016/j.jsbmb.2016.07.002. Epub 2016 Jul 5. Review.

PMID:
27392637
47.

A Novel NADPH-dependent flavoprotein reductase from Bacillus megaterium acts as an efficient cytochrome P450 reductase.

Milhim M, Gerber A, Neunzig J, Hannemann F, Bernhardt R.

J Biotechnol. 2016 Aug 10;231:83-94. doi: 10.1016/j.jbiotec.2016.05.035. Epub 2016 May 26.

PMID:
27238232
48.

Structure-function analysis for the hydroxylation of Δ4 C21-steroids by the myxobacterial CYP260B1.

Salamanca-Pinzon SG, Khatri Y, Carius Y, Keller L, Müller R, Lancaster CR, Bernhardt R.

FEBS Lett. 2016 Jun;590(12):1838-51. doi: 10.1002/1873-3468.12217. Epub 2016 Jun 3.

49.

Sulfated hyaluronan improves bone regeneration of diabetic rats by binding sclerostin and enhancing osteoblast function.

Picke AK, Salbach-Hirsch J, Hintze V, Rother S, Rauner M, Kascholke C, Möller S, Bernhardt R, Rammelt S, Pisabarro MT, Ruiz-Gómez G, Schnabelrauch M, Schulz-Siegmund M, Hacker MC, Scharnweber D, Hofbauer C, Hofbauer LC.

Biomaterials. 2016 Jul;96:11-23. doi: 10.1016/j.biomaterials.2016.04.013. Epub 2016 Apr 21.

PMID:
27131598
50.

Biotransformation of the mineralocorticoid receptor antagonists spironolactone and canrenone by human CYP11B1 and CYP11B2: Characterization of the products and their influence on mineralocorticoid receptor transactivation.

Schiffer L, Müller AR, Hobler A, Brixius-Anderko S, Zapp J, Hannemann F, Bernhardt R.

J Steroid Biochem Mol Biol. 2016 Oct;163:68-76. doi: 10.1016/j.jsbmb.2016.04.004. Epub 2016 Apr 25.

PMID:
27125452

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