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Items: 1 to 50 of 131

1.

Rat Organic Cation Transporter 1 Contains Three Binding Sites for Substrate 1-Methyl-4-phenylpyridinium per Monomer.

Keller T, Gorboulev V, Mueller T, Dotsch V, Bernhard F, Koepsell H.

Mol Pharmacol. 2018 Nov 8. pii: mol.118.113498. doi: 10.1124/mol.118.113498. [Epub ahead of print]

2.

Structural and functional insights into the interaction and targeting hub TMD0 of the polypeptide transporter TAPL.

Bock C, Löhr F, Tumulka F, Reichel K, Würz J, Hummer G, Schäfer L, Tampé R, Joseph B, Bernhard F, Dötsch V, Abele R.

Sci Rep. 2018 Oct 23;8(1):15662. doi: 10.1038/s41598-018-33841-w.

3.

Molecular Determinants for Ligand Selectivity of the Cell-Free Synthesized Human Endothelin B Receptor.

Dong F, Rues RB, Kazemi S, Dötsch V, Bernhard F.

J Mol Biol. 2018 Dec 7;430(24):5105-5119. doi: 10.1016/j.jmb.2018.10.006. Epub 2018 Oct 18.

PMID:
30342934
4.

Parkinson's disease: evolution of cognitive impairment and CSF Aβ1-42 profiles in a prospective longitudinal study.

Lerche S, Wurster I, Röben B, Machetanz G, Zimmermann M, Bernhard F, Stransky E, Deuschle C, Schulte C, Hansson O, Zetterberg H, Gasser T, Berg D, Maetzler W, Brockmann K.

J Neurol Neurosurg Psychiatry. 2018 Sep 25. pii: jnnp-2018-318956. doi: 10.1136/jnnp-2018-318956. [Epub ahead of print]

PMID:
30254084
5.

Lipid Conversion by Cell-Free Synthesized Phospholipid Methyltransferase Opi3 in Defined Nanodisc Membranes Supports an in Trans Mechanism.

Henrich E, Löhr F, Pawlik G, Peetz O, Dötsch V, Morgner N, de Kroon AI, Bernhard F.

Biochemistry. 2018 Oct 9;57(40):5780-5784. doi: 10.1021/acs.biochem.8b00807. Epub 2018 Sep 24.

PMID:
30226041
6.

LILBID and nESI: Different Native Mass Spectrometry Techniques as Tools in Structural Biology.

Peetz O, Hellwig N, Henrich E, Mezhyrova J, Dötsch V, Bernhard F, Morgner N.

J Am Soc Mass Spectrom. 2018 Sep 17. doi: 10.1007/s13361-018-2061-4. [Epub ahead of print]

PMID:
30225732
7.

[Validation of the Geriatric Check in a cohort of hospitalized neurological patients].

Hobert MA, Bernhard FP, Bettecken K, Sartor J, Maetzler W, Jamour M.

Z Gerontol Geriatr. 2018 Sep 11. doi: 10.1007/s00391-018-1441-5. [Epub ahead of print] German.

PMID:
30206742
8.

Wearables for gait and balance assessment in the neurological ward - study design and first results of a prospective cross-sectional feasibility study with 384 inpatients.

Bernhard FP, Sartor J, Bettecken K, Hobert MA, Arnold C, Weber YG, Poli S, Margraf NG, Schlenstedt C, Hansen C, Maetzler W.

BMC Neurol. 2018 Aug 16;18(1):114. doi: 10.1186/s12883-018-1111-7.

9.

The Effect of Microgravity on Central Aortic Blood Pressure.

Seibert FS, Bernhard F, Stervbo U, Vairavanathan S, Bauer F, Rohn B, Pagonas N, Babel N, Jankowski J, Westhoff TH.

Am J Hypertens. 2018 Oct 15;31(11):1183-1189. doi: 10.1093/ajh/hpy119.

PMID:
30052726
10.

Precursor-Based Selective Methyl Labeling of Cell-Free Synthesized Proteins.

Lazarova M, Löhr F, Rues RB, Kleebach R, Dötsch V, Bernhard F.

ACS Chem Biol. 2018 Aug 17;13(8):2170-2178. doi: 10.1021/acschembio.8b00338. Epub 2018 Jun 22.

PMID:
29894149
11.

Protein labeling strategies for liquid-state NMR spectroscopy using cell-free synthesis.

Hoffmann B, Löhr F, Laguerre A, Bernhard F, Dötsch V.

Prog Nucl Magn Reson Spectrosc. 2018 Apr;105:1-22. doi: 10.1016/j.pnmrs.2017.11.004. Epub 2017 Dec 7. Review.

PMID:
29548364
12.

Systematic optimization of cell-free synthesized human endothelin B receptor folding.

Rues RB, Dong F, Dötsch V, Bernhard F.

Methods. 2018 Sep 1;147:73-83. doi: 10.1016/j.ymeth.2018.01.012. Epub 2018 Feb 2.

PMID:
29378315
13.

The soluble loop BC region guides, but not dictates, the assembly of the transmembrane cytochrome b6.

Tome-Stangl L, Schaetzel C, Tenzer S, Bernhard F, Schneider D.

PLoS One. 2017 Dec 14;12(12):e0189532. doi: 10.1371/journal.pone.0189532. eCollection 2017.

14.

Corrigendum: White Matter Changes-Related Gait and Executive Function Deficits: Associations with Age and Parkinson's Disease.

Sartor J, Bettecken K, Bernhard FP, Hofmann M, Gladow T, Lindig T, Ciliz M, Ten Kate M, Geritz J, Heinzel S, Benedictus M, Scheltens P, Hobert MA, Maetzler W.

Front Aging Neurosci. 2017 Oct 17;9:342. doi: 10.3389/fnagi.2017.00342. eCollection 2017.

15.

Insights into Cotranslational Membrane Protein Insertion by Combined LILBID-Mass Spectrometry and NMR Spectroscopy.

Peetz O, Henrich E, Laguerre A, Löhr F, Hein C, Dötsch V, Bernhard F, Morgner N.

Anal Chem. 2017 Nov 21;89(22):12314-12318. doi: 10.1021/acs.analchem.7b03309. Epub 2017 Oct 30.

PMID:
29039652
16.

The E. coli S30 lysate proteome: A prototype for cell-free protein production.

Foshag D, Henrich E, Hiller E, Schäfer M, Kerger C, Burger-Kentischer A, Diaz-Moreno I, García-Mauriño SM, Dötsch V, Rupp S, Bernhard F.

N Biotechnol. 2018 Jan 25;40(Pt B):245-260. doi: 10.1016/j.nbt.2017.09.005. Epub 2017 Sep 21.

PMID:
28943390
17.

White Matter Changes-Related Gait and Executive Function Deficits: Associations with Age and Parkinson's Disease.

Sartor J, Bettecken K, Bernhard FP, Hofmann M, Gladow T, Lindig T, Ciliz M, Ten Kate M, Geritz J, Heinzel S, Benedictus M, Scheltens P, Hobert MA, Maetzler W.

Front Aging Neurosci. 2017 Jun 30;9:213. doi: 10.3389/fnagi.2017.00213. eCollection 2017. Erratum in: Front Aging Neurosci. 2017 Oct 17;9:342.

18.

Cell-free protein synthesis in micro compartments: building a minimal cell from biobricks.

Jia H, Heymann M, Bernhard F, Schwille P, Kai L.

N Biotechnol. 2017 Oct 25;39(Pt B):199-205. doi: 10.1016/j.nbt.2017.06.014. Epub 2017 Jul 6. Review.

PMID:
28690157
19.

Progression markers of motor deficits in Parkinson's disease: A biannual 4-year prospective study.

Heinzel S, Bernhard FP, Roeben B, Nussbaum S, Heger T, Martus P, Hobert MA, Maetzler W, Berg D.

Mov Disord. 2017 Aug;32(8):1254-1256. doi: 10.1002/mds.27062. Epub 2017 Jul 6. No abstract available.

PMID:
28681985
20.

No relevant association of kinematic gait parameters with Health-related Quality of Life in Parkinson's disease.

Bettecken K, Bernhard F, Sartor J, Hobert MA, Hofmann M, Gladow T, van Uem JMT, Liepelt-Scarfone I, Maetzler W.

PLoS One. 2017 May 22;12(5):e0176816. doi: 10.1371/journal.pone.0176816. eCollection 2017.

21.

Membrane Protein Production in E. coli Lysates in Presence of Preassembled Nanodiscs.

Rues RB, Gräwe A, Henrich E, Bernhard F.

Methods Mol Biol. 2017;1586:291-312. doi: 10.1007/978-1-4939-6887-9_19.

PMID:
28470613
22.

From Gene to Function: Cell-Free Electrophysiological and Optical Analysis of Ion Pumps in Nanodiscs.

Henrich E, Sörmann J, Eberhardt P, Peetz O, Mezhyrova J, Morgner N, Fendler K, Dötsch V, Wachtveitl J, Bernhard F, Bamann C.

Biophys J. 2017 Sep 19;113(6):1331-1341. doi: 10.1016/j.bpj.2017.03.026. Epub 2017 Apr 24.

23.

Analyzing native membrane protein assembly in nanodiscs by combined non-covalent mass spectrometry and synthetic biology.

Henrich E, Peetz O, Hein C, Laguerre A, Hoffmann B, Hoffmann J, Dötsch V, Bernhard F, Morgner N.

Elife. 2017 Jan 9;6. pii: e20954. doi: 10.7554/eLife.20954.

24.

The synaptic vesicle protein SV31 assembles into a dimer and transports Zn2.

Waberer L, Henrich E, Peetz O, Morgner N, Dötsch V, Bernhard F, Volknandt W.

J Neurochem. 2017 Jan;140(2):280-293. doi: 10.1111/jnc.13886. Epub 2016 Dec 5.

25.

From Nanodiscs to Isotropic Bicelles: A Procedure for Solution Nuclear Magnetic Resonance Studies of Detergent-Sensitive Integral Membrane Proteins.

Laguerre A, Löhr F, Henrich E, Hoffmann B, Abdul-Manan N, Connolly PJ, Perozo E, Moore JM, Bernhard F, Dötsch V.

Structure. 2016 Oct 4;24(10):1830-1841. doi: 10.1016/j.str.2016.07.017. Epub 2016 Sep 15.

26.

Cell-Free Production of Membrane Proteins in Escherichia coli Lysates for Functional and Structural Studies.

Rues RB, Henrich E, Boland C, Caffrey M, Bernhard F.

Methods Mol Biol. 2016;1432:1-21. doi: 10.1007/978-1-4939-3637-3_1.

PMID:
27485326
27.

Combining in Vitro Folding with Cell Free Protein Synthesis for Membrane Protein Expression.

Focke PJ, Hein C, Hoffmann B, Matulef K, Bernhard F, Dötsch V, Valiyaveetil FI.

Biochemistry. 2016 Aug 2;55(30):4212-9. doi: 10.1021/acs.biochem.6b00488. Epub 2016 Jul 21.

28.

Insulin-Like Growth Factor 1 (IGF-1) in Parkinson's Disease: Potential as Trait-, Progression- and Prediction Marker and Confounding Factors.

Bernhard FP, Heinzel S, Binder G, Weber K, Apel A, Roeben B, Deuschle C, Maechtel M, Heger T, Nussbaum S, Gasser T, Maetzler W, Berg D.

PLoS One. 2016 Mar 11;11(3):e0150552. doi: 10.1371/journal.pone.0150552. eCollection 2016.

29.

Co-translational formation and pharmacological characterization of beta1-adrenergic receptor/nanodisc complexes with different lipid environments.

Rues RB, Dötsch V, Bernhard F.

Biochim Biophys Acta. 2016 Jun;1858(6):1306-16. doi: 10.1016/j.bbamem.2016.02.031. Epub 2016 Feb 26.

30.

Lipid Requirements for the Enzymatic Activity of MraY Translocases and in Vitro Reconstitution of the Lipid II Synthesis Pathway.

Henrich E, Ma Y, Engels I, Münch D, Otten C, Schneider T, Henrichfreise B, Sahl HG, Dötsch V, Bernhard F.

J Biol Chem. 2016 Jan 29;291(5):2535-46. doi: 10.1074/jbc.M115.664292. Epub 2015 Nov 30.

31.

Labeling of membrane proteins by cell-free expression.

LaGuerre A, Löhr F, Bernhard F, Dötsch V.

Methods Enzymol. 2015;565:367-88. doi: 10.1016/bs.mie.2015.06.001. Epub 2015 Jul 2.

PMID:
26577739
32.

Biosynthesis of membrane dependent proteins in insect cell lysates: identification of limiting parameters for folding and processing.

Merk H, Rues RB, Gless C, Beyer K, Dong F, Dötsch V, Gerrits M, Bernhard F.

Biol Chem. 2015 Sep;396(9-10):1097-107. doi: 10.1515/hsz-2015-0105.

PMID:
25999328
33.

Membrane protein production in Escherichia coli cell-free lysates.

Henrich E, Hein C, Dötsch V, Bernhard F.

FEBS Lett. 2015 Jul 8;589(15):1713-22. doi: 10.1016/j.febslet.2015.04.045. Epub 2015 May 1. Review.

34.

Screening for lipid requirements of membrane proteins by combining cell-free expression with nanodiscs.

Henrich E, Dötsch V, Bernhard F.

Methods Enzymol. 2015;556:351-69. doi: 10.1016/bs.mie.2014.12.016. Epub 2015 Mar 20.

PMID:
25857790
35.

Cell-free expression of G-protein-coupled receptors.

Orbán E, Proverbio D, Haberstock S, Dötsch V, Bernhard F.

Methods Mol Biol. 2015;1261:171-95. doi: 10.1007/978-1-4939-2230-7_10.

PMID:
25502200
36.

Naturally occurring alpha-synuclein autoantibodies in Parkinson's disease: sources of (error) variance in biomarker assays.

Heinzel S, Gold M, Deuschle C, Bernhard F, Maetzler W, Berg D, Dodel R.

PLoS One. 2014 Dec 3;9(12):e114566. doi: 10.1371/journal.pone.0114566. eCollection 2014.

37.

Co-translational stabilization of insoluble proteins in cell-free expression systems.

Kai L, Orbán E, Henrich E, Proverbio D, Dötsch V, Bernhard F.

Methods Mol Biol. 2015;1258:125-43. doi: 10.1007/978-1-4939-2205-5_7. Review.

PMID:
25447862
38.

Cell-free expression of G-protein coupled receptors: new pipelines for challenging targets.

Rues RB, Orbán E, Dötsch V, Bernhard F.

Biol Chem. 2014 Dec;395(12):1425-34. doi: 10.1515/hsz-2014-0217. Review.

PMID:
25178907
39.

Cell-free expression and in meso crystallisation of an integral membrane kinase for structure determination.

Boland C, Li D, Shah STA, Haberstock S, Dötsch V, Bernhard F, Caffrey M.

Cell Mol Life Sci. 2014 Dec;71(24):4895-4910. doi: 10.1007/s00018-014-1655-7. Epub 2014 Jul 11.

40.

High-level cell-free production of membrane proteins with nanodiscs.

Roos C, Kai L, Haberstock S, Proverbio D, Ghoshdastider U, Ma Y, Filipek S, Wang X, Dötsch V, Bernhard F.

Methods Mol Biol. 2014;1118:109-30. doi: 10.1007/978-1-62703-782-2_7.

PMID:
24395412
41.

Conformational stabilization of the membrane embedded targeting domain of the lysosomal peptide transporter TAPL for solution NMR.

Tumulka F, Roos C, Löhr F, Bock C, Bernhard F, Dötsch V, Abele R.

J Biomol NMR. 2013 Oct;57(2):141-54. doi: 10.1007/s10858-013-9774-2. Epub 2013 Sep 7.

PMID:
24013930
42.

Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artificial membrane environments.

Proverbio D, Roos C, Beyermann M, Orbán E, Dötsch V, Bernhard F.

Biochim Biophys Acta. 2013 Sep;1828(9):2182-92. doi: 10.1016/j.bbamem.2013.05.031. Epub 2013 Jun 6.

43.

Cell-free expression--making a mark.

Bernhard F, Tozawa Y.

Curr Opin Struct Biol. 2013 Jun;23(3):374-80. doi: 10.1016/j.sbi.2013.03.012. Epub 2013 Apr 27. Review.

PMID:
23628286
44.

Artificial environments for the co-translational stabilization of cell-free expressed proteins.

Kai L, Dötsch V, Kaldenhoff R, Bernhard F.

PLoS One. 2013;8(2):e56637. doi: 10.1371/journal.pone.0056637. Epub 2013 Feb 22.

45.

Modified lipid and protein dynamics in nanodiscs.

Mörs K, Roos C, Scholz F, Wachtveitl J, Dötsch V, Bernhard F, Glaubitz C.

Biochim Biophys Acta. 2013 Apr;1828(4):1222-9. doi: 10.1016/j.bbamem.2012.12.011. Epub 2012 Dec 28.

46.

Functional relevance of genes implicated by obesity genome-wide association study signals for human adipocyte biology.

Bernhard F, Landgraf K, Klöting N, Berthold A, Büttner P, Friebe D, Kiess W, Kovacs P, Blüher M, Körner A.

Diabetologia. 2013 Feb;56(2):311-22. doi: 10.1007/s00125-012-2773-0. Epub 2012 Nov 16.

PMID:
23229156
47.

Cell-free expression of human glucosamine 6-phosphate N-acetyltransferase (HsGNA1) for inhibitor screening.

Ma Y, Ghoshdastider U, Wang J, Ye W, Dötsch V, Filipek S, Bernhard F, Wang X.

Protein Expr Purif. 2012 Dec;86(2):120-6. doi: 10.1016/j.pep.2012.09.011. Epub 2012 Oct 2.

PMID:
23036358
48.

Characterization of co-translationally formed nanodisc complexes with small multidrug transporters, proteorhodopsin and with the E. coli MraY translocase.

Roos C, Zocher M, Müller D, Münch D, Schneider T, Sahl HG, Scholz F, Wachtveitl J, Ma Y, Proverbio D, Henrich E, Dötsch V, Bernhard F.

Biochim Biophys Acta. 2012 Dec;1818(12):3098-106. doi: 10.1016/j.bbamem.2012.08.007. Epub 2012 Aug 18.

49.

Co-translational association of cell-free expressed membrane proteins with supplied lipid bilayers.

Roos C, Kai L, Proverbio D, Ghoshdastider U, Filipek S, Dötsch V, Bernhard F.

Mol Membr Biol. 2013 Feb;30(1):75-89. doi: 10.3109/09687688.2012.693212. Epub 2012 Jun 20.

PMID:
22716775
50.

A systematic approach to increase the efficiency of membrane protein production in cell-free expression systems.

Haberstock S, Roos C, Hoevels Y, Dötsch V, Schnapp G, Pautsch A, Bernhard F.

Protein Expr Purif. 2012 Apr;82(2):308-16. doi: 10.1016/j.pep.2012.01.018. Epub 2012 Feb 8.

PMID:
22342679

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