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Items: 42

1.

Intestinal Enteroendocrine Lineage Cells Possess Homeostatic and Injury-Inducible Stem Cell Activity.

Yan KS, Gevaert O, Zheng GXY, Anchang B, Probert CS, Larkin KA, Davies PS, Cheng ZF, Kaddis JS, Han A, Roelf K, Calderon RI, Cynn E, Hu X, Mandleywala K, Wilhelmy J, Grimes SM, Corney DC, Boutet SC, Terry JM, Belgrader P, Ziraldo SB, Mikkelsen TS, Wang F, von Furstenberg RJ, Smith NR, Chandrakesan P, May R, Chrissy MAS, Jain R, Cartwright CA, Niland JC, Hong YK, Carrington J, Breault DT, Epstein J, Houchen CW, Lynch JP, Martin MG, Plevritis SK, Curtis C, Ji HP, Li L, Henning SJ, Wong MH, Kuo CJ.

Cell Stem Cell. 2017 Jul 6;21(1):78-90.e6. doi: 10.1016/j.stem.2017.06.014.

2.

Non-equivalence of Wnt and R-spondin ligands during Lgr5+ intestinal stem-cell self-renewal.

Yan KS, Janda CY, Chang J, Zheng GXY, Larkin KA, Luca VC, Chia LA, Mah AT, Han A, Terry JM, Ootani A, Roelf K, Lee M, Yuan J, Li X, Bolen CR, Wilhelmy J, Davies PS, Ueno H, von Furstenberg RJ, Belgrader P, Ziraldo SB, Ordonez H, Henning SJ, Wong MH, Snyder MP, Weissman IL, Hsueh AJ, Mikkelsen TS, Garcia KC, Kuo CJ.

Nature. 2017 May 11;545(7653):238-242. doi: 10.1038/nature22313. Epub 2017 May 3.

3.

Massively parallel digital transcriptional profiling of single cells.

Zheng GX, Terry JM, Belgrader P, Ryvkin P, Bent ZW, Wilson R, Ziraldo SB, Wheeler TD, McDermott GP, Zhu J, Gregory MT, Shuga J, Montesclaros L, Underwood JG, Masquelier DA, Nishimura SY, Schnall-Levin M, Wyatt PW, Hindson CM, Bharadwaj R, Wong A, Ness KD, Beppu LW, Deeg HJ, McFarland C, Loeb KR, Valente WJ, Ericson NG, Stevens EA, Radich JP, Mikkelsen TS, Hindson BJ, Bielas JH.

Nat Commun. 2017 Jan 16;8:14049. doi: 10.1038/ncomms14049.

4.

Haplotyping germline and cancer genomes with high-throughput linked-read sequencing.

Zheng GX, Lau BT, Schnall-Levin M, Jarosz M, Bell JM, Hindson CM, Kyriazopoulou-Panagiotopoulou S, Masquelier DA, Merrill L, Terry JM, Mudivarti PA, Wyatt PW, Bharadwaj R, Makarewicz AJ, Li Y, Belgrader P, Price AD, Lowe AJ, Marks P, Vurens GM, Hardenbol P, Montesclaros L, Luo M, Greenfield L, Wong A, Birch DE, Short SW, Bjornson KP, Patel P, Hopmans ES, Wood C, Kaur S, Lockwood GK, Stafford D, Delaney JP, Wu I, Ordonez HS, Grimes SM, Greer S, Lee JY, Belhocine K, Giorda KM, Heaton WH, McDermott GP, Bent ZW, Meschi F, Kondov NO, Wilson R, Bernate JA, Gauby S, Kindwall A, Bermejo C, Fehr AN, Chan A, Saxonov S, Ness KD, Hindson BJ, Ji HP.

Nat Biotechnol. 2016 Mar;34(3):303-11. doi: 10.1038/nbt.3432. Epub 2016 Feb 1.

5.

Akonni TruTip(®) and Qiagen(®) methods for extraction of fetal circulating DNA--evaluation by real-time and digital PCR.

Holmberg RC, Gindlesperger A, Stokes T, Lopez D, Hyman L, Freed M, Belgrader P, Harvey J, Li Z.

PLoS One. 2013 Aug 6;8(8):e73068. doi: 10.1371/journal.pone.0073068. Print 2013.

6.

High-throughput, automated extraction of DNA and RNA from clinical samples using TruTip technology on common liquid handling robots.

Holmberg RC, Gindlesperger A, Stokes T, Brady D, Thakore N, Belgrader P, Cooney CG, Chandler DP.

J Vis Exp. 2013 Jun 11;(76):e50356. doi: 10.3791/50356.

7.

Detection of methicillin-resistant Staphylococcus aureus by a duplex droplet digital PCR assay.

Kelley K, Cosman A, Belgrader P, Chapman B, Sullivan DC.

J Clin Microbiol. 2013 Jul;51(7):2033-9. doi: 10.1128/JCM.00196-13. Epub 2013 Apr 17.

8.

Droplet digital PCR measurement of HER2 copy number alteration in formalin-fixed paraffin-embedded breast carcinoma tissue.

Belgrader P, Tanner SC, Regan JF, Koehler R, Hindson BJ, Brown AS.

Clin Chem. 2013 Jun;59(6):991-4. doi: 10.1373/clinchem.2012.197855. Epub 2013 Jan 28.

9.

Droplet Digital™ PCR quantitation of HER2 expression in FFPE breast cancer samples.

Heredia NJ, Belgrader P, Wang S, Koehler R, Regan J, Cosman AM, Saxonov S, Hindson B, Tanner SC, Brown AS, Karlin-Neumann G.

Methods. 2013 Jan;59(1):S20-3. doi: 10.1016/j.ymeth.2012.09.012. Epub 2012 Oct 2.

PMID:
23036330
10.

Rapid, simple influenza RNA extraction from nasopharyngeal samples.

Chandler DP, Griesemer SB, Cooney CG, Holmberg R, Thakore N, Mokhiber B, Belgrader P, Knickerbocker C, Schied J, St George K.

J Virol Methods. 2012 Jul;183(1):8-13. doi: 10.1016/j.jviromet.2012.03.002. Epub 2012 Mar 7.

11.

High-throughput droplet digital PCR system for absolute quantitation of DNA copy number.

Hindson BJ, Ness KD, Masquelier DA, Belgrader P, Heredia NJ, Makarewicz AJ, Bright IJ, Lucero MY, Hiddessen AL, Legler TC, Kitano TK, Hodel MR, Petersen JF, Wyatt PW, Steenblock ER, Shah PH, Bousse LJ, Troup CB, Mellen JC, Wittmann DK, Erndt NG, Cauley TH, Koehler RT, So AP, Dube S, Rose KA, Montesclaros L, Wang S, Stumbo DP, Hodges SP, Romine S, Milanovich FP, White HE, Regan JF, Karlin-Neumann GA, Hindson CM, Saxonov S, Colston BW.

Anal Chem. 2011 Nov 15;83(22):8604-10. doi: 10.1021/ac202028g. Epub 2011 Oct 28.

12.

A plastic, disposable microfluidic flow cell for coupled on-chip PCR and microarray detection of infectious agents.

Cooney CG, Sipes D, Thakore N, Holmberg R, Belgrader P.

Biomed Microdevices. 2012 Feb;14(1):45-53. doi: 10.1007/s10544-011-9584-9.

13.

A reusable flow-through polymerase chain reaction instrument for the continuous monitoring of infectious biological agents.

Belgrader P, Elkin CJ, Brown SB, Nasarabadi SN, Langlois RG, Milanovich FP, Colston BW Jr, Marshall GD.

Anal Chem. 2003 Jul 15;75(14):3446-50.

PMID:
14570196
14.

Microchip isoelectric focusing using a miniature scanning detection system.

Raisi F, Belgrader P, Borkholder DA, Herr AE, Kintz GJ, Pourhamadi F, Taylor MT, Northrup MA.

Electrophoresis. 2001 Jul;22(11):2291-5.

PMID:
11504064
15.

Lysing bacterial spores by sonication through a flexible interface in a microfluidic system.

Taylor MT, Belgrader P, Furman BJ, Pourahmadi F, Kovacs GT, Northrup MA.

Anal Chem. 2001 Feb 1;73(3):492-6.

PMID:
11217752
16.

A battery-powered notebook thermal cycler for rapid multiplex real-time PCR analysis.

Belgrader P, Young S, Yuan B, Primeau M, Christel LA, Pourahmadi F, Northrup MA.

Anal Chem. 2001 Jan 15;73(2):286-9. Erratum in: Anal Chem 2001 Jan 15;73(2):391.

PMID:
11199979
17.

Toward a rapid, integrated, and fully automated DNA diagnostic assay for chlamydia trachomatis and neisseria gonorrhoeae

Pourahmadi F, Taylor M, Kovacs G, Lloyd K, Sakai S, Schafer T, Helton B, Western L, Zaner S, Ching J, McMillan B, Belgrader P, Northrup MA.

Clin Chem. 2000 Sep;46(9):1511-3. No abstract available.

18.

A microfluidic cartridge to prepare spores for PCR analysis.

Belgrader P, Okuzumi M, Pourahmadi F, Borkholder DA, Northrup MA.

Biosens Bioelectron. 2000 Jan;14(10-11):849-52.

PMID:
10945459
19.

Simultaneous detection of TaqMan probes containing Fam and Tamra reporter fluorophores.

Nasarabadi S, Milanovich F, Richards J, Belgrader P.

Biotechniques. 1999 Dec;27(6):1116-8. No abstract available.

PMID:
10631486
20.

A minisonicator to rapidly disrupt bacterial spores for DNA analysis.

Belgrader P, Hansford D, Kovacs GT, Venkateswaran K, Mariella R Jr, Milanovich F, Nasarabadi S, Okuzumi M, Pourahmadi F, Northrup MA.

Anal Chem. 1999 Oct 1;71(19):4232-6.

PMID:
10517145
21.

PCR detection of bacteria in seven minutes.

Belgrader P, Benett W, Hadley D, Richards J, Stratton P, Mariella R Jr, Milanovich F.

Science. 1999 Apr 16;284(5413):449-50. No abstract available.

PMID:
10232992
22.

Rapid pathogen detection using a microchip PCR array instrument.

Belgrader P, Benett W, Hadley D, Long G, Mariella R Jr, Milanovich F, Nasarabadi S, Nelson W, Richards J, Stratton P.

Clin Chem. 1998 Oct;44(10):2191-4.

23.
24.

Real-time microchip PCR for detecting single-base differences in viral and human DNA.

Ibrahim MS, Lofts RS, Jahrling PB, Henchal EA, Weedn VW, Northrup MA, Belgrader P.

Anal Chem. 1998 May 1;70(9):2013-7.

PMID:
9599592
25.

Rapid PCR for identity testing using a battery-powered miniature thermal cycler.

Belgrader P, Smith JK, Weedn VW, Northrup MA.

J Forensic Sci. 1998 Mar;43(2):315-9.

PMID:
9544539
26.

DNA typing of human leukocyte antigen sequence polymorphisms by peptide nucleic acid probes and MALDI-TOF mass spectrometry.

Jiang-Baucom P, Girard JE, Butler J, Belgrader P.

Anal Chem. 1997 Dec 1;69(23):4894-8.

PMID:
9406536
27.
28.
29.

DNA typing in thirty seconds with a microfabricated device.

Schmalzing D, Koutny L, Adourian A, Belgrader P, Matsudaira P, Ehrlich D.

Proc Natl Acad Sci U S A. 1997 Sep 16;94(19):10273-8.

30.

Peptide nucleic acid characterization by MALDI-TOF mass spectrometry.

Butler JM, Jiang-Baucom P, Huang M, Belgrader P, Girard J.

Anal Chem. 1996 Sep 15;68(18):3283-7.

PMID:
8797387
31.
32.

Automated polymerase chain reaction product sample preparation for capillary electrophoresis analysis.

Belgrader P, Devaney JM, Del Rio SA, Turner KA, Weaver KR, Marino MA.

J Chromatogr B Biomed Appl. 1996 Aug 9;683(1):109-14.

PMID:
8876445
33.

Reusing the same blood-stained punch for sequential DNA amplifications and typing.

Del Rio SA, Marino MA, Belgrader P.

Biotechniques. 1996 Jun;20(6):970-2, 974. No abstract available.

34.

PCR-based human leukocyte antigen (HLA) DQ alpha typing of blood stained light and dark blue denim fabric.

Del Rio SA, Marino MA, Belgrader P.

J Forensic Sci. 1996 May;41(3):490-2.

PMID:
8656191
35.

The evaluation of fast purification methods for preparing polymerase chain reaction (PCR) products for capillary electrophoresis analysis.

Devaney JM, Marino MA, Williams PE, Weaver KR, Del Rio SA, Turner KA, Belgrader P.

Appl Theor Electrophor. 1996;6(1):11-4.

PMID:
9072075
36.

Automated DNA purification and amplification from blood-stained cards using a robotic workstation.

Belgrader P, Del Rio SA, Turner KA, Marino MA, Weaver KR, Williams PE.

Biotechniques. 1995 Sep;19(3):426-32.

PMID:
7495556
37.

Mammalian nonsense codons can be cis effectors of nuclear mRNA half-life.

Belgrader P, Cheng J, Zhou X, Stephenson LS, Maquat LE.

Mol Cell Biol. 1994 Dec;14(12):8219-28.

39.

Introns are cis effectors of the nonsense-codon-mediated reduction in nuclear mRNA abundance.

Cheng J, Belgrader P, Zhou X, Maquat LE.

Mol Cell Biol. 1994 Sep;14(9):6317-25.

41.
42.

A comprehensive study on the isolation and characterization of the HeLa S3 nuclear matrix.

Belgrader P, Siegel AJ, Berezney R.

J Cell Sci. 1991 Mar;98 ( Pt 3):281-91.

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