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Items: 1 to 50 of 129

1.

Structural and functional characterization of Escherichia coli UMP kinase in complex with its allosteric regulator GTP.

Meyer P, Evrin C, Briozzo P, Joly N, Bârzu O, Gilles AM.

J Biol Chem. 2008 Dec 19;283(51):36011-8. doi: 10.1074/jbc.M802614200. Epub 2008 Oct 22.

2.

Regulatory mechanisms differ in UMP kinases from gram-negative and gram-positive bacteria.

Evrin C, Straut M, Slavova-Azmanova N, Bucurenci N, Onu A, Assairi L, Ionescu M, Palibroda N, Bârzu O, Gilles AM.

J Biol Chem. 2007 Mar 9;282(10):7242-53. Epub 2007 Jan 8.

3.

Conversion of methionine to cysteine in Bacillus subtilis and its regulation.

Hullo MF, Auger S, Soutourina O, Barzu O, Yvon M, Danchin A, Martin-Verstraete I.

J Bacteriol. 2007 Jan;189(1):187-97. Epub 2006 Oct 20.

4.

Structure of Escherichia coli UMP kinase differs from that of other nucleoside monophosphate kinases and sheds new light on enzyme regulation.

Briozzo P, Evrin C, Meyer P, Assairi L, Joly N, Barzu O, Gilles AM.

J Biol Chem. 2005 Jul 8;280(27):25533-40. Epub 2005 Apr 27.

5.

High-level production of recombinant sulfide-reactive hemoglobin I from Lucina pectinata in Escherichia coli. High yields of fully functional holoprotein synthesis in the BLi5 E. coli strain.

León RG, Munier-Lehmann H, Barzu O, Baudin-Creuza V, Pietri R, López-Garriga J, Cadilla CL.

Protein Expr Purif. 2004 Dec;38(2):184-95.

PMID:
15555934
6.

Structure-function relationships of UMP kinases from pyrH mutants of Gram-negative bacteria.

Sakamoto H, Landais S, Evrin C, Laurent-Winter C, Bârzu O, Kelln RA.

Microbiology. 2004 Jul;150(Pt 7):2153-9.

PMID:
15256558
7.

Deciphering the function of an ORF: Salmonella enterica DeoM protein is a new mutarotase specific for deoxyribose.

Assairi L, Bertrand T, Ferdinand J, Slavova-Azmanova N, Christensen M, Briozzo P, Schaeffer F, Craescu CT, Neuhard J, Bârzu O, Gilles AM.

Protein Sci. 2004 May;13(5):1295-303. Epub 2004 Apr 9.

8.

Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy.

Gallay J, Vincent M, Li de la Sierra IM, Munier-Lehmann H, Renouard M, Sakamoto H, Bârzu O, Gilles AM.

Eur J Biochem. 2004 Feb;271(4):821-33. Erratum in: Eur J Biochem. 2004 Jun;271(11):2291.

9.

Regulation of expression of the 2-deoxy-D-ribose utilization regulon, deoQKPX, from Salmonella enterica serovar typhimurium.

Christensen M, Borza T, Dandanell G, Gilles AM, Barzu O, Kelln RA, Neuhard J.

J Bacteriol. 2003 Oct;185(20):6042-50.

10.

Relationship between bacterial virulence and nucleotide metabolism: a mutation in the adenylate kinase gene renders Yersinia pestis avirulent.

Munier-Lehmann H, Chenal-Francisque V, Ionescu M, Chrisova P, Foulon J, Carniel E, Bârzu O.

Biochem J. 2003 Jul 15;373(Pt 2):515-22.

11.

UMP kinase from the Gram-positive bacterium Bacillus subtilis is strongly dependent on GTP for optimal activity.

Gagyi C, Bucurenci N, Sîrbu O, Labesse G, Ionescu M, Ofiteru A, Assairi L, Landais S, Danchin A, Bârzu O, Gilles AM.

Eur J Biochem. 2003 Aug;270(15):3196-204.

12.

Structural and nucleotide-binding properties of YajQ and YnaF, two Escherichia coli proteins of unknown function.

Saveanu C, Miron S, Borza T, Craescu CT, Labesse G, Gagyi C, Popescu A, Schaeffer F, Namane A, Laurent-Winter C, Bârzu O, Gilles AM.

Protein Sci. 2002 Nov;11(11):2551-60.

13.

Comparative modelling and immunochemical reactivity of Escherichia coli UMP kinase.

Labesse G, Bucurenci N, Douguet D, Sakamoto H, Landais S, Gagyi C, Gilles AM, Bârzu O.

Biochem Biophys Res Commun. 2002 May 31;294(1):173-9.

PMID:
12054759
14.

Sugar specificity of bacterial CMP kinases as revealed by crystal structures and mutagenesis of Escherichia coli enzyme.

Bertrand T, Briozzo P, Assairi L, Ofiteru A, Bucurenci N, Munier-Lehmann H, Golinelli-Pimpaneau B, Bârzu O, Gilles AM.

J Mol Biol. 2002 Feb 1;315(5):1099-110.

PMID:
11827479
15.

1H, 13C and 15N resonance assignment of YajQ, a protein of unknown structure and function from Escherichia coli.

Miron S, Borza T, Saveanu C, Gilles AM, Bârzu O, Craescu CT.

J Biomol NMR. 2001 Jul;20(3):287-8. No abstract available.

PMID:
11519751
16.

X-ray structure of TMP kinase from Mycobacterium tuberculosis complexed with TMP at 1.95 A resolution.

Li de la Sierra I, Munier-Lehmann H, Gilles AM, Bârzu O, Delarue M.

J Mol Biol. 2001 Aug 3;311(1):87-100.

PMID:
11469859
17.

Substrate-induced fit of the ATP binding site of cytidine monophosphate kinase from Escherichia coli: time-resolved fluorescence of 3'-anthraniloyl-2'-deoxy-ADP and molecular modeling.

Li de La Sierra IM, Gallay J, Vincent M, Bertrand T, Briozzo P, Bârzu O, Gilles AM.

Biochemistry. 2000 Dec 26;39(51):15870-8.

PMID:
11123913
18.

Crystallization and preliminary X-ray analysis of the thymidylate kinase from Mycobacterium tuberculosis.

Li de la Sierra I, Munier-Lehmann H, Gilles AM, Bârzu O, Delarue M.

Acta Crystallogr D Biol Crystallogr. 2000 Feb;56(Pt 2):226-8.

PMID:
10666613
19.

Genetic and biochemical characterization of Salmonella enterica serovar typhi deoxyribokinase.

Tourneux L, Bucurenci N, Saveanu C, Kaminski PA, Bouzon M, Pistotnik E, Namane A, Marlière P, Bârzu O, Li De La Sierra I, Neuhard J, Gilles AM.

J Bacteriol. 2000 Feb;182(4):869-73.

20.

Decreased capacity of recombinant 45/47-kDa molecules (Apa) of Mycobacterium tuberculosis to stimulate T lymphocyte responses related to changes in their mannosylation pattern.

Horn C, Namane A, Pescher P, Rivière M, Romain F, Puzo G, Bârzu O, Marchal G.

J Biol Chem. 1999 Nov 5;274(45):32023-30.

21.
22.

The highly similar TMP kinases of Yersinia pestis and Escherichia coli differ markedly in their AZTMP phosphorylating activity.

Chenal-Francisque V, Tourneux L, Carniel E, Christova P, Li de la Sierra I, Bârzu O, Gilles AM.

Eur J Biochem. 1999 Oct 1;265(1):112-9.

23.
24.

Assay of nucleoside 2-deoxyribosyltransferase activity with pyruvate kinase/lactate dehydrogenase coupling system.

Pistotnik E, Sakamoto H, Pochet S, Namane A, Bârzu O.

Anal Biochem. 1999 Jul 1;271(2):192-3. No abstract available.

PMID:
10419637
25.

1H, 13C and 15N backbone resonance assignment of Escherichia coli adenylate kinase, a 23.6 kDa protein.

Burlacu-Miron S, Perrier V, Gilles AM, Mispelter J, Bârzu O, Craescu CT.

J Biomol NMR. 1999 Jan;13(1):93-4. No abstract available.

PMID:
10070751
26.

Immunochemical analysis of UMP kinase from Escherichia coli.

Landais S, Gounon P, Laurent-Winter C, Mazié JC, Danchin A, Bârzu O, Sakamoto H.

J Bacteriol. 1999 Feb;181(3):833-40.

27.

Structures of escherichia coli CMP kinase alone and in complex with CDP: a new fold of the nucleoside monophosphate binding domain and insights into cytosine nucleotide specificity.

Briozzo P, Golinelli-Pimpaneau B, Gilles AM, Gaucher JF, Burlacu-Miron S, Sakamoto H, Janin J, Bârzu O.

Structure. 1998 Dec 15;6(12):1517-27.

28.

Mutational analysis of UMP kinase from Escherichia coli.

Bucurenci N, Serina L, Zaharia C, Landais S, Danchin A, Bârzu O.

J Bacteriol. 1998 Feb;180(3):473-7.

29.
30.

Borrelia burgdorferi uridine kinase: an enzyme of the pyrimidine salvage pathway for endogenous use of nucleotides.

Boursaux-Eude C, Margarita D, Gilles AM, Bârzu O, Saint Girons I.

FEMS Microbiol Lett. 1997 Jun 15;151(2):257-61.

31.

Structural and catalytic properties of CMP kinase from Bacillus subtilis: a comparative analysis with the homologous enzyme from Escherichia coli.

Schultz CP, Ylisastigui-Pons L, Serina L, Sakamoto H, Mantsch HH, Neuhard J, Bârzu O, Gilles AM.

Arch Biochem Biophys. 1997 Apr 1;340(1):144-53.

PMID:
9126287
32.

Characterization of metal and nucleotide liganded forms of adenylate kinase by electrospray ionization mass spectrometry.

Briand G, Perrier V, Kouach M, Takahashi M, Gilles AM, Bârzu O.

Arch Biochem Biophys. 1997 Mar 15;339(2):291-7.

PMID:
9056261
33.

Structural properties of UMP-kinase from Escherichia coli: modulation of protein solubility by pH and UTP.

Serina L, Bucurenci N, Gilles AM, Surewicz WK, Fabian H, Mantsch HH, Takahashi M, Petrescu I, Batelier G, Bârzu O.

Biochemistry. 1996 Jun 4;35(22):7003-11.

PMID:
8679525
34.

Conformational transitions within the calmodulin-binding site of Bordetella pertussis adenylate cyclase studied by time-resolved fluorescence of Trp242 and circular dichroism.

Bouhss A, Vincent M, Munier H, Gilles AM, Takahashi M, Bârzu O, Danchin A, Gallay J.

Eur J Biochem. 1996 May 1;237(3):619-28.

35.

CMP kinase from Escherichia coli is structurally related to other nucleoside monophosphate kinases.

Bucurenci N, Sakamoto H, Briozzo P, Palibroda N, Serina L, Sarfati RS, Labesse G, Briand G, Danchin A, Bărzu O, Gilles AM.

J Biol Chem. 1996 Feb 2;271(5):2856-62.

36.

Chemienzymatic synthesis of uridine nucleotides labeled with [15N] and [13C].

Gilles AM, Cristea I, Palibroda N, Hilden I, Jensen KF, Sarfati RS, Namane A, Ughetto-Monfrin J, Bârzu O.

Anal Biochem. 1995 Dec 10;232(2):197-203.

PMID:
8747475
37.

Structural characterization by nuclear magnetic resonance spectroscopy of a genetically engineered high-affinity calmodulin-binding peptide derived from Bordetella pertussis adenylate cyclase.

Munier H, Bouhss A, Gilles AM, Palibroda N, Bârzu O, Mispelter J, Craescu CT.

Arch Biochem Biophys. 1995 Jul 10;320(2):224-35.

PMID:
7625828
38.

Escherichia coli UMP-kinase, a member of the aspartokinase family, is a hexamer regulated by guanine nucleotides and UTP.

Serina L, Blondin C, Krin E, Sismeiro O, Danchin A, Sakamoto H, Gilles AM, Bârzu O.

Biochemistry. 1995 Apr 18;34(15):5066-74.

PMID:
7711027
39.

Calmodulin binding of a peptide derived from the regulatory domain of Bordetella pertussis adenylate cyclase.

Craescu CT, Bouhss A, Mispelter J, Diesis E, Popescu A, Chiriac M, Bârzu O.

J Biol Chem. 1995 Mar 31;270(13):7088-96.

40.

Enzymatic synthesis of guanine nucleotides labeled with 15N at the 2-amino group of the purine ring.

Bouhss A, Sakamoto H, Palibroda N, Chiriac M, Sarfati R, Smith JM, Craescu CT, Bârzu O.

Anal Biochem. 1995 Feb 10;225(1):18-23.

PMID:
7778777
41.

Zinc chelation and structural stability of adenylate kinase from Bacillus subtilis.

Perrier V, Surewicz WK, Glaser P, Martineau L, Craescu CT, Fabian H, Mantsch HH, Bârzu O, Gilles AM.

Biochemistry. 1994 Aug 23;33(33):9960-7.

PMID:
8061005
42.

Improved spectrophotometric assay of nucleoside monophosphate kinase activity using the pyruvate kinase/lactate dehydrogenase coupling system.

Blondin C, Serina L, Wiesmüller L, Gilles AM, Bârzu O.

Anal Biochem. 1994 Jul;220(1):219-21. No abstract available.

PMID:
7978251
43.

Zinc, a structural component of adenylate kinases from gram-positive bacteria.

Gilles AM, Glaser P, Perrier V, Meier A, Longin R, Sebald M, Maignan L, Pistotnik E, Bârzu O.

J Bacteriol. 1994 Jan;176(2):520-3.

44.

Adenylyl cyclases: a heterogeneous class of ATP-utilizing enzymes.

Bârzu O, Danchin A.

Prog Nucleic Acid Res Mol Biol. 1994;49:241-83. Review. No abstract available.

PMID:
7863008
45.

Structural and physico-chemical characteristics of Bordetella pertussis adenylate kinase, a tryptophan-containing enzyme.

Gilles AM, Sismeiro O, Munier H, Fabian H, Mantsch HH, Surewicz WK, Craescu CC, Barzu O, Danchin A.

Eur J Biochem. 1993 Dec 15;218(3):921-7.

47.

Asparagine-135 of elongation factor Tu is a crucial residue for the folding of the guanine nucleotide binding pocket.

Weijland A, Sarfati R, Bârzu O, Parmeggiani A.

FEBS Lett. 1993 Sep 20;330(3):334-8.

48.

Unipolar localization and ATPase activity of IcsA, a Shigella flexneri protein involved in intracellular movement.

Goldberg MB, Barzu O, Parsot C, Sansonetti PJ.

Infect Agents Dis. 1993 Aug;2(4):210-1. No abstract available.

PMID:
8173795
49.

Unipolar localization and ATPase activity of IcsA, a Shigella flexneri protein involved in intracellular movement.

Goldberg MB, Bârzu O, Parsot C, Sansonetti PJ.

J Bacteriol. 1993 Apr;175(8):2189-96.

50.

Characterization of a synthetic calmodulin-binding peptide derived from Bacillus anthracis adenylate cyclase.

Munier H, Blanco FJ, Prêcheur B, Diesis E, Nieto JL, Craescu CT, Bârzu O.

J Biol Chem. 1993 Jan 25;268(3):1695-701.

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