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Items: 1 to 50 of 92

1.

Optimizing locked nucleic acid/2'-O-methyl-RNA fluorescence in situ hybridization (LNA/2'OMe-FISH) procedure for bacterial detection.

Azevedo AS, Sousa IM, Fernandes RM, Azevedo NF, Almeida C.

PLoS One. 2019 May 31;14(5):e0217689. doi: 10.1371/journal.pone.0217689. eCollection 2019.

2.

Propidium iodide staining underestimates viability of adherent bacterial cells.

Rosenberg M, Azevedo NF, Ivask A.

Sci Rep. 2019 Apr 24;9(1):6483. doi: 10.1038/s41598-019-42906-3.

3.

Application of agent-based modelling to assess single-molecule transport across the cell envelope of E. coli.

Maia P, Pérez-Rodríguez G, Pérez-Pérez M, Fdez-Riverola F, Lourenço A, Azevedo NF.

Comput Biol Med. 2019 Apr;107:218-226. doi: 10.1016/j.compbiomed.2019.02.020. Epub 2019 Mar 2.

PMID:
30852248
4.

Development and application of Peptide Nucleic Acid Fluorescence in situ Hybridization for the specific detection of Listeria monocytogenes.

Rocha R, Sousa JM, Cerqueira L, Vieira MJ, Almeida C, Azevedo NF.

Food Microbiol. 2019 Jun;80:1-8. doi: 10.1016/j.fm.2018.12.009. Epub 2018 Dec 15.

PMID:
30704592
5.

Correction: Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria.

Rocha R, Almeida C, Azevedo NF.

PLoS One. 2018 Dec 5;13(12):e0208867. doi: 10.1371/journal.pone.0208867. eCollection 2018.

6.

Validation of Biomode S.A. Probe4CronobacterTM for the Identification of Cronobacter spp.

Sousa JM, Rocha R, Cerqueira L, Almeida C, Azevedo NF, Bastin B, Bird P, Benzinger MJ, Agin J, Goins D, Chen Y, Brodsky M, Odumoru J.

J AOAC Int. 2019 May 1;102(3):855-864. doi: 10.5740/jaoacint.18-0328. Epub 2018 Nov 20.

PMID:
30458901
7.

Eco-friendly non-biocide-release coatings for marine biofouling prevention.

Silva ER, Ferreira O, Ramalho PA, Azevedo NF, Bayón R, Igartua A, Bordado JC, Calhorda MJ.

Sci Total Environ. 2019 Feb 10;650(Pt 2):2499-2511. doi: 10.1016/j.scitotenv.2018.10.010. Epub 2018 Oct 2.

PMID:
30293004
8.

Quantitative assessment of individual populations within polymicrobial biofilms.

Lopes SP, Azevedo NF, Pereira MO.

Sci Rep. 2018 Jun 22;8(1):9494. doi: 10.1038/s41598-018-27497-9.

9.

Targeting miR-9 in gastric cancer cells using locked nucleic acid oligonucleotides.

Lima JF, Carvalho J, Pinto-Ribeiro I, Almeida C, Wengel J, Cerqueira L, Figueiredo C, Oliveira C, Azevedo NF.

BMC Mol Biol. 2018 Jun 7;19(1):6. doi: 10.1186/s12867-018-0107-6.

10.

Influence of the fixation/permeabilization step on peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria.

Rocha R, Almeida C, Azevedo NF.

PLoS One. 2018 May 31;13(5):e0196522. doi: 10.1371/journal.pone.0196522. eCollection 2018. Erratum in: PLoS One. 2018 Dec 5;13(12):e0208867.

11.

Anti-miRNA oligonucleotides: A comprehensive guide for design.

Lima JF, Cerqueira L, Figueiredo C, Oliveira C, Azevedo NF.

RNA Biol. 2018 Mar 4;15(3):338-352. doi: 10.1080/15476286.2018.1445959. Epub 2018 Mar 23. Review.

12.

Agent-based model of diffusion of N-acyl homoserine lactones in a multicellular environment of Pseudomonas aeruginosa and Candida albicans.

Pérez-Rodríguez G, Dias S, Pérez-Pérez M, Fdez-Riverola F, Azevedo NF, Lourenço A.

Biofouling. 2018 Mar;34(3):335-345. doi: 10.1080/08927014.2018.1440392. Epub 2018 Mar 8.

PMID:
29516751
13.

Surface modifications for antimicrobial effects in the healthcare setting: a critical overview.

Adlhart C, Verran J, Azevedo NF, Olmez H, Keinänen-Toivola MM, Gouveia I, Melo LF, Crijns F.

J Hosp Infect. 2018 Jul;99(3):239-249. doi: 10.1016/j.jhin.2018.01.018. Epub 2018 Feb 2. Review.

14.

Nanomaterials and molecular transporters to overcome the bacterial envelope barrier: Towards advanced delivery of antibiotics.

Santos RS, Figueiredo C, Azevedo NF, Braeckmans K, De Smedt SC.

Adv Drug Deliv Rev. 2018 Nov - Dec;136-137:28-48. doi: 10.1016/j.addr.2017.12.010. Epub 2017 Dec 14. Review.

PMID:
29248479
15.

Developing a model for cystic fibrosis sociomicrobiology based on antibiotic and environmental stress.

Lopes SP, Azevedo NF, Pereira MO.

Int J Med Microbiol. 2017 Dec;307(8):460-470. doi: 10.1016/j.ijmm.2017.09.018. Epub 2017 Sep 28.

PMID:
29033313
16.

Morphological transition of Helicobacter pylori adapted to water.

Fernandes RM, Silva H, Oliveira R, Almeida C, Azevedo NF, Vieira MJ.

Future Microbiol. 2017 Oct;12:1167-1179. doi: 10.2217/fmb-2016-0174. Epub 2017 Sep 7.

PMID:
28879777
17.

It is all about location: how to pinpoint microorganisms and their functions in multispecies biofilms.

Costa AM, Mergulhão FJ, Briandet R, Azevedo NF.

Future Microbiol. 2017 Sep;12:987-999. doi: 10.2217/fmb-2017-0053. Epub 2017 Jul 26.

PMID:
28745517
18.

Applications of optical DNA mapping in microbiology.

Bogas D, Nyberg L, Pacheco R, Azevedo NF, Beech JP, Gomila M, Lalucat J, Manaia CM, Nunes OC, Tegenfeldt JO, Westerlund F.

Biotechniques. 2017 Jun 1;62(6):255-267. doi: 10.2144/000114555.

19.

Detection of Helicobacter pylori in the Gastric Mucosa by Fluorescence In Vivo Hybridization.

Fontenete S, Leite M, Figueiredo C, Cos P, Azevedo NF.

Methods Mol Biol. 2017;1616:137-146. doi: 10.1007/978-1-4939-7037-7_8.

PMID:
28600766
20.

Intracellular delivery of oligonucleotides in Helicobacter pylori by fusogenic liposomes in the presence of gastric mucus.

Santos RS, Dakwar GR, Zagato E, Brans T, Figueiredo C, Raemdonck K, Azevedo NF, De Smedt SC, Braeckmans K.

Biomaterials. 2017 Sep;138:1-12. doi: 10.1016/j.biomaterials.2017.05.029. Epub 2017 May 20.

PMID:
28550752
21.

Knowledge about the human immunodeficiency syndrome among elders in a unit for the care of the elderly.

Nardelli GG, Malaquias BSS, Gaudenci EM, Ledic CS, Azevedo NF, Martins VE, Santos ÁDS.

Rev Gaucha Enferm. 2017 May 18;37(spe):e20160039. doi: 10.1590/1983-1447.2016.esp.2016-0039. Portuguese, English.

22.

Yeasts identification in microfluidic devices using peptide nucleic acid fluorescence in situ hybridization (PNA-FISH).

Ferreira AM, Cruz-Moreira D, Cerqueira L, Miranda JM, Azevedo NF.

Biomed Microdevices. 2017 Mar;19(1):11. doi: 10.1007/s10544-017-0150-y.

PMID:
28144839
23.

Polymicrobial Ventilator-Associated Pneumonia: Fighting In Vitro Candida albicans-Pseudomonas aeruginosa Biofilms with Antifungal-Antibacterial Combination Therapy.

Rodrigues ME, Lopes SP, Pereira CR, Azevedo NF, Lourenço A, Henriques M, Pereira MO.

PLoS One. 2017 Jan 23;12(1):e0170433. doi: 10.1371/journal.pone.0170433. eCollection 2017.

24.

Impact of polymicrobial biofilms in catheter-associated urinary tract infections.

Azevedo AS, Almeida C, Melo LF, Azevedo NF.

Crit Rev Microbiol. 2017 Aug;43(4):423-439. doi: 10.1080/1040841X.2016.1240656. Epub 2016 Dec 30. Review.

PMID:
28033847
25.

Critical review on biofilm methods.

Azeredo J, Azevedo NF, Briandet R, Cerca N, Coenye T, Costa AR, Desvaux M, Di Bonaventura G, Hébraud M, Jaglic Z, Kačániová M, Knøchel S, Lourenço A, Mergulhão F, Meyer RL, Nychas G, Simões M, Tresse O, Sternberg C.

Crit Rev Microbiol. 2017 May;43(3):313-351. doi: 10.1080/1040841X.2016.1208146. Epub 2016 Nov 21. Review.

PMID:
27868469
26.

Novel strategy to detect and locate periodontal pathogens: The PNA-FISH technique.

Mendes L, Rocha R, Azevedo AS, Ferreira C, Henriques M, Pinto MG, Azevedo NF.

Microbiol Res. 2016 Nov;192:185-191. doi: 10.1016/j.micres.2016.07.002. Epub 2016 Jul 14.

27.

Discriminating typical and atypical cystic fibrosis-related bacteria by multiplex PNA-FISH.

Lopes SP, Carvalho DT, Pereira MO, Azevedo NF.

Biotechnol Bioeng. 2017 Feb;114(2):355-367. doi: 10.1002/bit.26085. Epub 2016 Sep 26.

PMID:
27571488
28.

Single Molecule Simulation of Diffusion and Enzyme Kinetics.

Pérez-Rodríguez G, Gameiro D, Pérez-Pérez M, Lourenço A, Azevedo NF.

J Phys Chem B. 2016 Apr 28;120(16):3809-20. doi: 10.1021/acs.jpcb.5b12544. Epub 2016 Apr 13.

PMID:
27049044
29.

Optimization of peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) for the detection of bacteria: The effect of pH, dextran sulfate and probe concentration.

Rocha R, Santos RS, Madureira P, Almeida C, Azevedo NF.

J Biotechnol. 2016 May 20;226:1-7. doi: 10.1016/j.jbiotec.2016.03.047. Epub 2016 Mar 25.

PMID:
27021959
30.

Application of locked nucleic acid-based probes in fluorescence in situ hybridization.

Fontenete S, Carvalho D, Guimarães N, Madureira P, Figueiredo C, Wengel J, Azevedo NF.

Appl Microbiol Biotechnol. 2016 Jul;100(13):5897-906. doi: 10.1007/s00253-016-7429-4. Epub 2016 Mar 12.

PMID:
26969040
31.

Impact of Delftia tsuruhatensis and Achromobacter xylosoxidans on Escherichia coli dual-species biofilms treated with antibiotic agents.

Azevedo AS, Almeida C, Pereira B, Melo LF, Azevedo NF.

Biofouling. 2016;32(3):227-41. doi: 10.1080/08927014.2015.1124096.

PMID:
26901701
32.

Fluorescence In Vivo Hybridization (FIVH) for Detection of Helicobacter pylori Infection in a C57BL/6 Mouse Model.

Fontenete S, Leite M, Cappoen D, Santos R, Ginneken CV, Figueiredo C, Wengel J, Cos P, Azevedo NF.

PLoS One. 2016 Feb 5;11(2):e0148353. doi: 10.1371/journal.pone.0148353. eCollection 2016.

33.

Discrimination of bacteriophage infected cells using locked nucleic acid fluorescent in situ hybridization (LNA-FISH).

Vilas Boas D, Almeida C, Sillankorva S, Nicolau A, Azeredo J, Azevedo NF.

Biofouling. 2016;32(2):179-90. doi: 10.1080/08927014.2015.1131821. Erratum in: Biofouling. 2016;32(2):ii.

PMID:
26813295
34.

Effect of Native Gastric Mucus on in vivo Hybridization Therapies Directed at Helicobacter pylori.

Santos RS, Dakwar GR, Xiong R, Forier K, Remaut K, Stremersch S, Guimarães N, Fontenete S, Wengel J, Leite M, Figueiredo C, De Smedt SC, Braeckmans K, Azevedo NF.

Mol Ther Nucleic Acids. 2015 Dec 8;4:e269. doi: 10.1038/mtna.2015.46.

35.

The cystic fibrosis microbiome in an ecological perspective and its impact in antibiotic therapy.

Magalhães AP, Azevedo NF, Pereira MO, Lopes SP.

Appl Microbiol Biotechnol. 2016 Feb;100(3):1163-1181. doi: 10.1007/s00253-015-7177-x. Epub 2015 Dec 5. Review.

PMID:
26637419
36.

Computational resources and strategies to construct single-molecule metabolic models of microbial cells.

Gameiro D, Pérez-Pérez M, Pérez-Rodríguez G, Monteiro G, Azevedo NF, Lourenço A.

Brief Bioinform. 2016 Sep;17(5):863-76. doi: 10.1093/bib/bbv096. Epub 2015 Oct 28. Review.

PMID:
26515531
37.

Towards Fluorescence In Vivo Hybridization (FIVH) Detection of H. pylori in Gastric Mucosa Using Advanced LNA Probes.

Fontenete S, Leite M, Guimarães N, Madureira P, Ferreira RM, Figueiredo C, Wengel J, Azevedo NF.

PLoS One. 2015 Apr 27;10(4):e0125494. doi: 10.1371/journal.pone.0125494. eCollection 2015.

38.

Agent-based spatiotemporal simulation of biomolecular systems within the open source MASON framework.

Pérez-Rodríguez G, Pérez-Pérez M, Glez-Peña D, Fdez-Riverola F, Azevedo NF, Lourenço A.

Biomed Res Int. 2015;2015:769471. doi: 10.1155/2015/769471. Epub 2015 Mar 22.

39.

Erratum to: mismatch discrimination in fluorescent in situ hybridization using different types of nucleic acids.

Fontenete S, Barros J, Madureira P, Figueiredo C, Wengel J, Azevedo NF.

Appl Microbiol Biotechnol. 2015 May;99(9):4117. doi: 10.1007/s00253-015-6568-3. No abstract available.

PMID:
25846338
41.

Relationship between invasion of the periodontium by periodontal pathogens and periodontal disease: a systematic review.

Mendes L, Azevedo NF, Felino A, Pinto MG.

Virulence. 2015;6(3):208-15. doi: 10.4161/21505594.2014.984566. Review.

42.

Enabling systematic, harmonised and large-scale biofilms data computation: the Biofilms Experiment Workbench.

Pérez-Rodríguez G, Glez-Peña D, Azevedo NF, Pereira MO, Fdez-Riverola F, Lourenço A.

Comput Methods Programs Biomed. 2015 Mar;118(3):309-21. doi: 10.1016/j.cmpb.2014.12.005. Epub 2015 Jan 8.

PMID:
25600941
43.

Prediction of melting temperatures in fluorescence in situ hybridization (FISH) procedures using thermodynamic models.

Fontenete S, Guimarães N, Wengel J, Azevedo NF.

Crit Rev Biotechnol. 2016;36(3):566-77. doi: 10.3109/07388551.2014.993589. Epub 2015 Jan 14. Review.

PMID:
25586037
44.

A new colorimetric peptide nucleic acid-based assay for the specific detection of bacteria.

Almeida C, Constante D, Ferreira A, Cerqueira L, Vieira MJ, Azevedo NF.

Future Microbiol. 2014;9(10):1131-42. doi: 10.2217/fmb.14.68.

PMID:
25405883
45.

An harmonised vocabulary for communicating and interchanging biofilms experimental results.

Sousa AM, Pereira MO, Azevedo NF, Lourenço A.

J Integr Bioinform. 2014 Oct 23;11(3):249. doi: 10.2390/biecoll-jib-2014-249.

PMID:
25339083
46.

Interaction between atypical microorganisms and E. coli in catheter-associated urinary tract biofilms.

Azevedo AS, Almeida C, Melo LF, Azevedo NF.

Biofouling. 2014 Sep;30(8):893-902. doi: 10.1080/08927014.2014.944173.

PMID:
25184430
47.

Water-induced modulation of Helicobacter pylori virulence properties.

Guimarães NM, Azevedo NF, Vieira MJ, Figueiredo C.

Mem Inst Oswaldo Cruz. 2014 Jul;109(4):414-9.

48.

Optimization of a peptide nucleic acid fluorescence in situ hybridization (PNA-FISH) method for the detection of bacteria and disclosure of a formamide effect.

Santos RS, Guimarães N, Madureira P, Azevedo NF.

J Biotechnol. 2014 Oct 10;187:16-24. doi: 10.1016/j.jbiotec.2014.06.023. Epub 2014 Jul 14.

PMID:
25034435
49.

Detection of Dehalococcoides spp. by peptide nucleic acid fluorescent in situ hybridization.

Danko AS, Fontenete SJ, de Aquino Leite D, Leitão PO, Almeida C, Schaefer CE, Vainberg S, Steffan RJ, Azevedo NF.

J Mol Microbiol Biotechnol. 2014;24(3):142-9. doi: 10.1159/000362790. Epub 2014 Jun 26.

PMID:
24970105
50.

Emergent bacteria in cystic fibrosis: in vitro biofilm formation and resilience under variable oxygen conditions.

Lopes SP, Azevedo NF, Pereira MO.

Biomed Res Int. 2014;2014:678301. doi: 10.1155/2014/678301. Epub 2014 Apr 29.

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