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Items: 16

1.

Best practices and benchmarks for intact protein analysis for top-down mass spectrometry.

Donnelly DP, Rawlins CM, DeHart CJ, Fornelli L, Schachner LF, Lin Z, Lippens JL, Aluri KC, Sarin R, Chen B, Lantz C, Jung W, Johnson KR, Koller A, Wolff JJ, Campuzano IDG, Auclair JR, Ivanov AR, Whitelegge JP, Paša-Tolić L, Chamot-Rooke J, Danis PO, Smith LM, Tsybin YO, Loo JA, Ge Y, Kelleher NL, Agar JN.

Nat Methods. 2019 Jul;16(7):587-594. doi: 10.1038/s41592-019-0457-0. Epub 2019 Jun 27. Review.

PMID:
31249407
2.

Genetically Encoded Fluorescent Proteins Enable High-Throughput Assignment of Cell Cohorts Directly from MALDI-MS Images.

Schmitt ND, Rawlins CM, Randall EC, Wang X, Koller A, Auclair JR, Kowalski JM, Kowalski PJ, Luther E, Ivanov AR, Agar NYR, Agar JN.

Anal Chem. 2019 Mar 19;91(6):3810-3817. doi: 10.1021/acs.analchem.8b03454. Epub 2019 Mar 6.

PMID:
30839199
3.

Regulatory Convergence for Biologics through Capacity Building and Training.

Auclair JR.

Trends Biotechnol. 2019 Jan;37(1):5-9. doi: 10.1016/j.tibtech.2018.06.001. Epub 2018 Jun 20. Review.

PMID:
29935836
4.

Cyclic Thiosulfinates and Cyclic Disulfides Selectively Cross-Link Thiols While Avoiding Modification of Lone Thiols.

Donnelly DP, Dowgiallo MG, Salisbury JP, Aluri KC, Iyengar S, Chaudhari M, Mathew M, Miele I, Auclair JR, Lopez SA, Manetsch R, Agar JN.

J Am Chem Soc. 2018 Jun 20;140(24):7377-7380. doi: 10.1021/jacs.8b01136. Epub 2018 Jun 11.

PMID:
29851341
5.

Integrated Bottom-Up and Top-Down Liquid Chromatography-Mass Spectrometry for Characterization of Recombinant Human Growth Hormone Degradation Products.

Wang YA, Wu D, Auclair JR, Salisbury JP, Sarin R, Tang Y, Mozdzierz NJ, Shah K, Zhang AF, Wu SL, Agar JN, Love JC, Love KR, Hancock WS.

Anal Chem. 2017 Dec 5;89(23):12771-12777. doi: 10.1021/acs.analchem.7b03026. Epub 2017 Nov 21.

PMID:
29096433
6.

Heavy Sugar and Heavy Water Create Tunable Intact Protein Mass Increases for Quantitative Mass Spectrometry in Any Feed and Organism.

Quijada JV, Schmitt ND, Salisbury JP, Auclair JR, Agar JN.

Anal Chem. 2016 Nov 15;88(22):11139-11146. Epub 2016 Oct 28.

7.

Mildly acidic conditions eliminate deamidation artifact during proteolysis: digestion with endoprotease Glu-C at pH 4.5.

Liu S, Moulton KR, Auclair JR, Zhou ZS.

Amino Acids. 2016 Apr;48(4):1059-67. doi: 10.1007/s00726-015-2166-z. Epub 2016 Jan 9.

8.

The central nervous system transcriptome of the weakly electric brown ghost knifefish (Apteronotus leptorhynchus): de novo assembly, annotation, and proteomics validation.

Salisbury JP, Sîrbulescu RF, Moran BM, Auclair JR, Zupanc GK, Agar JN.

BMC Genomics. 2015 Mar 11;16:166. doi: 10.1186/s12864-015-1354-2.

9.

Identification of a misfolded region in superoxide dismutase 1 that is exposed in amyotrophic lateral sclerosis.

Rotunno MS, Auclair JR, Maniatis S, Shaffer SA, Agar J, Bosco DA.

J Biol Chem. 2014 Oct 10;289(41):28527-38. doi: 10.1074/jbc.M114.581801. Epub 2014 Aug 27.

10.

Artifacts to avoid while taking advantage of top-down mass spectrometry based detection of protein S-thiolation.

Auclair JR, Salisbury JP, Johnson JL, Petsko GA, Ringe D, Bosco DA, Agar NY, Santagata S, Durham HD, Agar JN.

Proteomics. 2014 May;14(10):1152-7. doi: 10.1002/pmic.201300450. Epub 2014 Apr 17.

11.

Post-translational modification by cysteine protects Cu/Zn-superoxide dismutase from oxidative damage.

Auclair JR, Johnson JL, Liu Q, Salisbury JP, Rotunno MS, Petsko GA, Ringe D, Brown RH Jr, Bosco DA, Agar JN.

Biochemistry. 2013 Sep 10;52(36):6137-44. doi: 10.1021/bi4006122. Epub 2013 Aug 26.

12.

Structural consequences of cysteinylation of Cu/Zn-superoxide dismutase.

Auclair JR, Brodkin HR, D'Aquino JA, Petsko GA, Ringe D, Agar JN.

Biochemistry. 2013 Sep 10;52(36):6145-50. doi: 10.1021/bi400613h. Epub 2013 Aug 26.

13.

Mass spectrometry tools for analysis of intermolecular interactions.

Auclair JR, Somasundaran M, Green KM, Evans JE, Schiffer CA, Ringe D, Petsko GA, Agar JN.

Methods Mol Biol. 2012;896:387-98. doi: 10.1007/978-1-4614-3704-8_26.

14.

A soluble α-synuclein construct forms a dynamic tetramer.

Wang W, Perovic I, Chittuluru J, Kaganovich A, Nguyen LT, Liao J, Auclair JR, Johnson D, Landeru A, Simorellis AK, Ju S, Cookson MR, Asturias FJ, Agar JN, Webb BN, Kang C, Ringe D, Petsko GA, Pochapsky TC, Hoang QQ.

Proc Natl Acad Sci U S A. 2011 Oct 25;108(43):17797-802. doi: 10.1073/pnas.1113260108. Epub 2011 Oct 17.

15.

Strategies for stabilizing superoxide dismutase (SOD1), the protein destabilized in the most common form of familial amyotrophic lateral sclerosis.

Auclair JR, Boggio KJ, Petsko GA, Ringe D, Agar JN.

Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21394-9. doi: 10.1073/pnas.1015463107. Epub 2010 Nov 22.

16.

Mass spectrometry analysis of HIV-1 Vif reveals an increase in ordered structure upon oligomerization in regions necessary for viral infectivity.

Auclair JR, Green KM, Shandilya S, Evans JE, Somasundaran M, Schiffer CA.

Proteins. 2007 Nov 1;69(2):270-84.

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