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Items: 13

1.

One step engineering of the small-subunit ribosomal RNA using CRISPR/Cas9.

Kannan K, Tsvetanova B, Chuang RY, Noskov VN, Assad-Garcia N, Ma L, Hutchison Iii CA, Smith HO, Glass JI, Merryman C, Venter JC, Gibson DG.

Sci Rep. 2016 Aug 4;6:30714. doi: 10.1038/srep30714.

2.

Design and synthesis of a minimal bacterial genome.

Hutchison CA 3rd, Chuang RY, Noskov VN, Assad-Garcia N, Deerinck TJ, Ellisman MH, Gill J, Kannan K, Karas BJ, Ma L, Pelletier JF, Qi ZQ, Richter RA, Strychalski EA, Sun L, Suzuki Y, Tsvetanova B, Wise KS, Smith HO, Glass JI, Merryman C, Gibson DG, Venter JC.

Science. 2016 Mar 25;351(6280):aad6253. doi: 10.1126/science.aad6253. Erratum in: ACS Chem Biol. 2016 May 20;11(5):1463.

PMID:
27013737
3.

Bacterial genome reduction using the progressive clustering of deletions via yeast sexual cycling.

Suzuki Y, Assad-Garcia N, Kostylev M, Noskov VN, Wise KS, Karas BJ, Stam J, Montague MG, Hanly TJ, Enriquez NJ, Ramon A, Goldgof GM, Richter RA, Vashee S, Chuang RY, Winzeler EA, Hutchison CA 3rd, Gibson DG, Smith HO, Glass JI, Venter JC.

Genome Res. 2015 Mar;25(3):435-44. doi: 10.1101/gr.182477.114. Epub 2015 Feb 5.

4.

A structurally distinct human mycoplasma protein that generically blocks antigen-antibody union.

Grover RK, Zhu X, Nieusma T, Jones T, Boreo I, MacLeod AS, Mark A, Niessen S, Kim HJ, Kong L, Assad-Garcia N, Kwon K, Chesi M, Smider VV, Salomon DR, Jelinek DF, Kyle RA, Pyles RB, Glass JI, Ward AB, Wilson IA, Lerner RA.

Science. 2014 Feb 7;343(6171):656-661. doi: 10.1126/science.1246135.

5.

A whole-cell computational model predicts phenotype from genotype.

Karr JR, Sanghvi JC, Macklin DN, Gutschow MV, Jacobs JM, Bolival B Jr, Assad-Garcia N, Glass JI, Covert MW.

Cell. 2012 Jul 20;150(2):389-401. doi: 10.1016/j.cell.2012.05.044.

6.

Enhancement of targeted homologous recombination in Mycoplasma mycoides subsp. capri by inclusion of heterologous recA.

Allam AB, Reyes L, Assad-Garcia N, Glass JI, Brown MB.

Appl Environ Microbiol. 2010 Oct;76(20):6951-4. doi: 10.1128/AEM.00056-10. Epub 2010 Aug 27.

7.

Targeted chromosomal knockouts in Mycoplasma pneumoniae.

Krishnakumar R, Assad-Garcia N, Benders GA, Phan Q, Montague MG, Glass JI.

Appl Environ Microbiol. 2010 Aug;76(15):5297-9. doi: 10.1128/AEM.00024-10. Epub 2010 Jun 11.

8.

Creation of a bacterial cell controlled by a chemically synthesized genome.

Gibson DG, Glass JI, Lartigue C, Noskov VN, Chuang RY, Algire MA, Benders GA, Montague MG, Ma L, Moodie MM, Merryman C, Vashee S, Krishnakumar R, Assad-Garcia N, Andrews-Pfannkoch C, Denisova EA, Young L, Qi ZQ, Segall-Shapiro TH, Calvey CH, Parmar PP, Hutchison CA 3rd, Smith HO, Venter JC.

Science. 2010 Jul 2;329(5987):52-6. doi: 10.1126/science.1190719. Epub 2010 May 20.

9.

Cloning whole bacterial genomes in yeast.

Benders GA, Noskov VN, Denisova EA, Lartigue C, Gibson DG, Assad-Garcia N, Chuang RY, Carrera W, Moodie M, Algire MA, Phan Q, Alperovich N, Vashee S, Merryman C, Venter JC, Smith HO, Glass JI, Hutchison CA 3rd.

Nucleic Acids Res. 2010 May;38(8):2558-69. doi: 10.1093/nar/gkq119. Epub 2010 Mar 7.

10.

Creating bacterial strains from genomes that have been cloned and engineered in yeast.

Lartigue C, Vashee S, Algire MA, Chuang RY, Benders GA, Ma L, Noskov VN, Denisova EA, Gibson DG, Assad-Garcia N, Alperovich N, Thomas DW, Merryman C, Hutchison CA 3rd, Smith HO, Venter JC, Glass JI.

Science. 2009 Sep 25;325(5948):1693-6. doi: 10.1126/science.1173759. Epub 2009 Aug 20.

11.

New selectable marker for manipulating the simple genomes of Mycoplasma species.

Algire MA, Lartigue C, Thomas DW, Assad-Garcia N, Glass JI, Merryman C.

Antimicrob Agents Chemother. 2009 Oct;53(10):4429-32. doi: 10.1128/AAC.00388-09. Epub 2009 Aug 17.

12.

Essential genes of a minimal bacterium.

Glass JI, Assad-Garcia N, Alperovich N, Yooseph S, Lewis MR, Maruf M, Hutchison CA 3rd, Smith HO, Venter JC.

Proc Natl Acad Sci U S A. 2006 Jan 10;103(2):425-30. Epub 2006 Jan 3.

13.

Agrobacterium-mediated transformation of tomatillo (Physalis ixocarpa) and tissue specific and developmental expression of the CaMV 35S promoter in transgenic tomatillo plants.

Assad-García N, Ochoa-Alejo N, García-Hernández E, Herrera-Estrella L, Simpson J.

Plant Cell Rep. 1992 Oct;11(11):558-62. doi: 10.1007/BF00233092.

PMID:
24213286

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