Sort by
Items per page

Send to

Choose Destination

Search results

Items: 1 to 50 of 58


Kinetochore-associated Stu2 promotes chromosome biorientation in vivo.

Miller MP, Evans RK, Zelter A, Geyer EA, MacCoss MJ, Rice LM, Davis TN, Asbury CL, Biggins S.

PLoS Genet. 2019 Oct 4;15(10):e1008423. doi: 10.1371/journal.pgen.1008423. eCollection 2019 Oct.


Autophosphorylation is sufficient to release Mps1 kinase from native kinetochores.

Koch LB, Opoku KN, Deng Y, Barber A, Littleton AJ, London N, Biggins S, Asbury CL.

Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17355-17360. doi: 10.1073/pnas.1901653116. Epub 2019 Aug 12.


Tight bending of the Ndc80 complex provides intrinsic regulation of its binding to microtubules.

Scarborough EA, Davis TN, Asbury CL.

Elife. 2019 May 2;8. pii: e44489. doi: 10.7554/eLife.44489.


Relax, Kinetochores Are Exquisitely Sensitive to Tension.

Larson JD, Asbury CL.

Dev Cell. 2019 Apr 8;49(1):5-7. doi: 10.1016/j.devcel.2019.03.019.


The kinetoplastid kinetochore protein KKT4 is an unconventional microtubule tip-coupling protein.

Llauró A, Hayashi H, Bailey ME, Wilson A, Ludzia P, Asbury CL, Akiyoshi B.

J Cell Biol. 2018 Nov 5;217(11):3886-3900. doi: 10.1083/jcb.201711181. Epub 2018 Sep 12.


Minimizing ATP depletion by oxygen scavengers for single-molecule fluorescence imaging in live cells.

Jung SR, Deng Y, Kushmerick C, Asbury CL, Hille B, Koh DS.

Proc Natl Acad Sci U S A. 2018 Jun 19;115(25):E5706-E5715. doi: 10.1073/pnas.1717724115. Epub 2018 Jun 4.


Human Ska complex and Ndc80 complex interact to form a load-bearing assembly that strengthens kinetochore-microtubule attachments.

Helgeson LA, Zelter A, Riffle M, MacCoss MJ, Asbury CL, Davis TN.

Proc Natl Acad Sci U S A. 2018 Mar 13;115(11):2740-2745. doi: 10.1073/pnas.1718553115. Epub 2018 Feb 27.


Direct measurement of conformational strain energy in protofilaments curling outward from disassembling microtubule tips.

Driver JW, Geyer EA, Bailey ME, Rice LM, Asbury CL.

Elife. 2017 Jun 19;6. pii: e28433. doi: 10.7554/eLife.28433.


Direct measurement of the strength of microtubule attachment to yeast centrosomes.

Fong KK, Sarangapani KK, Yusko EC, Riffle M, Llauró A, Graczyk B, Davis TN, Asbury CL.

Mol Biol Cell. 2017 Jul 7;28(14):1853-1861. doi: 10.1091/mbc.E17-01-0034. Epub 2017 Mar 22.


Anaphase A: Disassembling Microtubules Move Chromosomes toward Spindle Poles.

Asbury CL.

Biology (Basel). 2017 Feb 17;6(1). pii: E15. doi: 10.3390/biology6010015. Review.


The Ndc80 complex bridges two Dam1 complex rings.

Kim JO, Zelter A, Umbreit NT, Bollozos A, Riffle M, Johnson R, MacCoss MJ, Asbury CL, Davis TN.

Elife. 2017 Feb 13;6. pii: e21069. doi: 10.7554/eLife.21069.


Simultaneous Manipulation and Super-Resolution Fluorescence Imaging of Individual Kinetochores Coupled to Microtubule Tips.

Deng Y, Asbury CL.

Methods Mol Biol. 2017;1486:437-467. doi: 10.1007/978-1-4939-6421-5_17.


A TOG Protein Confers Tension Sensitivity to Kinetochore-Microtubule Attachments.

Miller MP, Asbury CL, Biggins S.

Cell. 2016 Jun 2;165(6):1428-1439. doi: 10.1016/j.cell.2016.04.030. Epub 2016 May 5.


Single-Molecule Total Internal Reflection Fluorescence Microscopy.

Kudalkar EM, Davis TN, Asbury CL.

Cold Spring Harb Protoc. 2016 May 2;2016(5). doi: 10.1101/pdb.top077800.


Data Analysis for Total Internal Reflection Fluorescence Microscopy.

Asbury CL.

Cold Spring Harb Protoc. 2016 May 2;2016(5). doi: 10.1101/pdb.prot085571.


Preparation of Reactions for Imaging with Total Internal Reflection Fluorescence Microscopy.

Kudalkar EM, Davis TN, Asbury CL.

Cold Spring Harb Protoc. 2016 May 2;2016(5). doi: 10.1101/pdb.prot085563.


Coverslip Cleaning and Functionalization for Total Internal Reflection Fluorescence Microscopy.

Kudalkar EM, Deng Y, Davis TN, Asbury CL.

Cold Spring Harb Protoc. 2016 May 2;2016(5). doi: 10.1101/pdb.prot085548.


Contributions of protein kinases and β-arrestin to termination of protease-activated receptor 2 signaling.

Jung SR, Seo JB, Deng Y, Asbury CL, Hille B, Koh DS.

J Gen Physiol. 2016 Mar;147(3):255-71. doi: 10.1085/jgp.201511477.


Regulation of outer kinetochore Ndc80 complex-based microtubule attachments by the central kinetochore Mis12/MIND complex.

Kudalkar EM, Scarborough EA, Umbreit NT, Zelter A, Gestaut DR, Riffle M, Johnson RS, MacCoss MJ, Asbury CL, Davis TN.

Proc Natl Acad Sci U S A. 2015 Oct 13;112(41):E5583-9. doi: 10.1073/pnas.1513882112. Epub 2015 Oct 1.


Force is a signal that cells cannot ignore.

Yusko EC, Asbury CL.

Mol Biol Cell. 2014 Nov 15;25(23):3717-25. doi: 10.1091/mbc.E13-12-0707. Review.


Kinetochores require oligomerization of Dam1 complex to maintain microtubule attachments against tension and promote biorientation.

Umbreit NT, Miller MP, Tien JF, Ortolá JC, Gui L, Lee KK, Biggins S, Asbury CL, Davis TN.

Nat Commun. 2014 Sep 19;5:4951. doi: 10.1038/ncomms5951.


Kinetochore biorientation in Saccharomyces cerevisiae requires a tightly folded conformation of the Ndc80 complex.

Tien JF, Umbreit NT, Zelter A, Riffle M, Hoopmann MR, Johnson RS, Fonslow BR, Yates JR 3rd, MacCoss MJ, Moritz RL, Asbury CL, Davis TN.

Genetics. 2014 Dec;198(4):1483-93. doi: 10.1534/genetics.114.167775. Epub 2014 Sep 16.


Sister kinetochores are mechanically fused during meiosis I in yeast.

Sarangapani KK, Duro E, Deng Y, Alves Fde L, Ye Q, Opoku KN, Ceto S, Rappsilber J, Corbett KD, Biggins S, Marston AL, Asbury CL.

Science. 2014 Oct 10;346(6206):248-51. doi: 10.1126/science.1256729. Epub 2014 Sep 11.


Catch and release: how do kinetochores hook the right microtubules during mitosis?

Sarangapani KK, Asbury CL.

Trends Genet. 2014 Apr;30(4):150-9. doi: 10.1016/j.tig.2014.02.004. Epub 2014 Mar 13. Review.


Measuring kinetochore-microtubule interaction in vitro.

Driver JW, Powers AF, Sarangapani KK, Biggins S, Asbury CL.

Methods Enzymol. 2014;540:321-37. doi: 10.1016/B978-0-12-397924-7.00018-2.


Coupling unbiased mutagenesis to high-throughput DNA sequencing uncovers functional domains in the Ndc80 kinetochore protein of Saccharomyces cerevisiae.

Tien JF, Fong KK, Umbreit NT, Payen C, Zelter A, Asbury CL, Dunham MJ, Davis TN.

Genetics. 2013 Sep;195(1):159-70. doi: 10.1534/genetics.113.152728. Epub 2013 Jul 5.


Phosphoregulation promotes release of kinetochores from dynamic microtubules via multiple mechanisms.

Sarangapani KK, Akiyoshi B, Duggan NM, Biggins S, Asbury CL.

Proc Natl Acad Sci U S A. 2013 Apr 30;110(18):7282-7. doi: 10.1073/pnas.1220700110. Epub 2013 Apr 15.


The Ndc80 kinetochore complex directly modulates microtubule dynamics.

Umbreit NT, Gestaut DR, Tien JF, Vollmar BS, Gonen T, Asbury CL, Davis TN.

Proc Natl Acad Sci U S A. 2012 Oct 2;109(40):16113-8. doi: 10.1073/pnas.1209615109. Epub 2012 Aug 20.


Kif18A and chromokinesins confine centromere movements via microtubule growth suppression and spatial control of kinetochore tension.

Stumpff J, Wagenbach M, Franck A, Asbury CL, Wordeman L.

Dev Cell. 2012 May 15;22(5):1017-29. doi: 10.1016/j.devcel.2012.02.013.


A tethering mechanism controls the processivity and kinetochore-microtubule plus-end enrichment of the kinesin-8 Kif18A.

Stumpff J, Du Y, English CA, Maliga Z, Wagenbach M, Asbury CL, Wordeman L, Ohi R.

Mol Cell. 2011 Sep 2;43(5):764-75. doi: 10.1016/j.molcel.2011.07.022.


Tension directly stabilizes reconstituted kinetochore-microtubule attachments.

Akiyoshi B, Sarangapani KK, Powers AF, Nelson CR, Reichow SL, Arellano-Santoyo H, Gonen T, Ranish JA, Asbury CL, Biggins S.

Nature. 2010 Nov 25;468(7323):576-9. doi: 10.1038/nature09594.


Kinetochores' gripping feat: conformational wave or biased diffusion?

Asbury CL, Tien JF, Davis TN.

Trends Cell Biol. 2011 Jan;21(1):38-46. doi: 10.1016/j.tcb.2010.09.003. Epub 2010 Oct 15. Review.


Kip3, the yeast kinesin-8, is required for clustering of kinetochores at metaphase.

Wargacki MM, Tay JC, Muller EG, Asbury CL, Davis TN.

Cell Cycle. 2010 Jul 1;9(13):2581-8.


Cooperation of the Dam1 and Ndc80 kinetochore complexes enhances microtubule coupling and is regulated by aurora B.

Tien JF, Umbreit NT, Gestaut DR, Franck AD, Cooper J, Wordeman L, Gonen T, Asbury CL, Davis TN.

J Cell Biol. 2010 May 17;189(4):713-23. doi: 10.1083/jcb.200910142.


Reconstitution and functional analysis of kinetochore subcomplexes.

Gestaut DR, Cooper J, Asbury CL, Davis TN, Wordeman L.

Methods Cell Biol. 2010;95:641-56. doi: 10.1016/S0091-679X(10)95032-2. Review.


Direct physical study of kinetochore-microtubule interactions by reconstitution and interrogation with an optical force clamp.

Franck AD, Powers AF, Gestaut DR, Davis TN, Asbury CL.

Methods. 2010 Jun;51(2):242-50. doi: 10.1016/j.ymeth.2010.01.020. Epub 2010 Jan 22. Review.


Catalysis of the microtubule on-rate is the major parameter regulating the depolymerase activity of MCAK.

Cooper JR, Wagenbach M, Asbury CL, Wordeman L.

Nat Struct Mol Biol. 2010 Jan;17(1):77-82. doi: 10.1038/nsmb.1728. Epub 2009 Dec 6.


On the origin of kinesin limping.

Fehr AN, Gutiérrez-Medina B, Asbury CL, Block SM.

Biophys J. 2009 Sep 16;97(6):1663-70. doi: 10.1016/j.bpj.2009.07.004.


The Ndc80 kinetochore complex forms load-bearing attachments to dynamic microtubule tips via biased diffusion.

Powers AF, Franck AD, Gestaut DR, Cooper J, Gracyzk B, Wei RR, Wordeman L, Davis TN, Asbury CL.

Cell. 2009 Mar 6;136(5):865-75. doi: 10.1016/j.cell.2008.12.045.


Insights into the kinetochore.

Asbury CL, Davis TN.

Structure. 2008 Jun;16(6):834-6. doi: 10.1016/j.str.2008.05.005.


Phosphoregulation and depolymerization-driven movement of the Dam1 complex do not require ring formation.

Gestaut DR, Graczyk B, Cooper J, Widlund PO, Zelter A, Wordeman L, Asbury CL, Davis TN.

Nat Cell Biol. 2008 Apr;10(4):407-14. doi: 10.1038/ncb1702. Epub 2008 Mar 23.


Chromosome bi-orientation: Euclidian euploidy.

Stumpff J, Asbury CL.

Curr Biol. 2008 Jan 22;18(2):R81-3. doi: 10.1016/j.cub.2007.11.036.


XMAP215: a tip tracker that really moves.

Asbury CL.

Cell. 2008 Jan 11;132(1):19-20. doi: 10.1016/j.cell.2007.12.019. Review.


Kinesin steps do not alternate in size.

Fehr AN, Asbury CL, Block SM.

Biophys J. 2008 Feb 1;94(3):L20-2. Epub 2007 Dec 14.


Tension applied through the Dam1 complex promotes microtubule elongation providing a direct mechanism for length control in mitosis.

Franck AD, Powers AF, Gestaut DR, Gonen T, Davis TN, Asbury CL.

Nat Cell Biol. 2007 Jul;9(7):832-7. Epub 2007 Jun 17.


The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement.

Asbury CL, Gestaut DR, Powers AF, Franck AD, Davis TN.

Proc Natl Acad Sci U S A. 2006 Jun 27;103(26):9873-8. Epub 2006 Jun 15.


Kinesin: world's tiniest biped.

Asbury CL.

Curr Opin Cell Biol. 2005 Feb;17(1):89-97. Review.


The polarization of fluorescence of DNA stains depends on the incorporation density of the dye molecules.

Uy JL, Asbury CL, Petersen TW, van den Engh G.

Cytometry A. 2004 Sep;61(1):18-25.


Kinesin moves by an asymmetric hand-over-hand mechanism.

Asbury CL, Fehr AN, Block SM.

Science. 2003 Dec 19;302(5653):2130-4. Epub 2003 Dec 4.


Probing the kinesin reaction cycle with a 2D optical force clamp.

Block SM, Asbury CL, Shaevitz JW, Lang MJ.

Proc Natl Acad Sci U S A. 2003 Mar 4;100(5):2351-6. Epub 2003 Feb 18.

Supplemental Content

Loading ...
Support Center