Send to

Choose Destination
Sex Transm Infect. 2018 Nov;94(7):479-482. doi: 10.1136/sextrans-2017-053502. Epub 2018 Apr 19.

Quality assurance for antimicrobial susceptibility testing of Neisseria gonorrhoeae in Latin American and Caribbean countries, 2013-2015.

Author information

Streptococcus and STI Unit, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Servicio de Enfermedades deTransmisión Sexual, Centro Nacional de Referencia en ITS, INEI-ANLIS 'Dr. Carlos G. Malbrán', Buenos Aires, Argentina.
Programa de ITS/VIH/SIDA, Laboratorio del Centro Departamental de Vigilancia, Informacion y Referencia, La Paz, Bolivia.
Bacteriology section,Infectious Diseases Sub-Department, Biomedical Department, Instituto de Salud Publica de Chile, Santiago, Chile.
Grupo de Microbiología, Instituto National de Salud, Bogota, Colombia.
Laboratorio Nacional de Referencia de Microbiología, Instituto de Medicina Tropical 'Pedro Kouri' (IPK), La Habana, Cuba.
Seccion Antimicrobianos, Laboratorio Central De Salud Pública, Asunción, Paraguay.
Laboratorio de Microbiologia, Departamento deBiociencias, Facultad de Quimica, Universidad de la Republica, Montevideo, Uruguay.
Departamento de Bacteriologia, Instituto Nacional de Higine 'Rafael Rangel', Caracas, Venezuela.
Laboratorio Central de Referencia en Salud Publica, Instituto Conmemeorahvo Gorgas de Estudios de la Salud, Panamá, República de Panamá.
Facultad de Salud Pública y Administración, Universidad Peruana Cayetano Heredia, Lima, Peru.
Department of Microbiology and Immunology, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.



A Neisseria gonorrhoeae antimicrobial susceptibility quality control comparison programme was re-established in Latin America and the Caribbean to ensure antimicrobial susceptibility data produced from the region are comparable nationally and internationally.


Three panels, consisting of N. gonorrhoeae isolates comprising reference strains and other characterised isolates were sent to 11 participating laboratories between 2013 and 2015. Antimicrobial susceptibilities for these isolates were determined using agar dilution, Etest or disc diffusion methods. Modal minimum inhibitory concentrations (MICs) for each panel isolate/antibiotic combination were calculated. The guidelines of the Clinical and Laboratory Standards Institute were used for interpretations of antimicrobial susceptibility. The agreement of MICs with the modal MICs was determined for each of the participating laboratories as well as for each of the antibiotics tested.


Five of 11 laboratories that participated in at least one panel had an overall average agreement between participants' MIC results and modal MICs of >90%. For other laboratories, agreements ranged from 60.0% to 82.4%. The proportion of agreement between interpretations for all the antibiotics, except penicillin and tetracycline, was >90%. The percentages of agreement between MIC results and their modes for erythromycin, spectinomycin, cefixime and azithromycin were >90%. Tetracycline, ceftriaxone and ciprofloxacin agreement ranged from 84.5% to 89.1%, while penicillin had 78.8% agreement between MICs and modal MICs.


The participating laboratories had acceptable results, similar to other international quality assurance programmes. It is important to ensure continuation of the International Gonococcal Antimicrobial Susceptibility Quality Control Comparison Programme to ensure that participants can identify and correct any problems in antimicrobial susceptibility testing for N. gonorrhoeae as they arise and continue to generate reproducible and reliable data.


Latin America; antimicrobial resistance; gonorrhoea; surveillance

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for HighWire
Loading ...
Support Center