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Items: 37


Enhancement of the Anti-Aggregation Activity of a Molecular Chaperone Using a Rationally Designed Post-Translational Modification.

Lindstedt PR, Aprile FA, Matos MJ, Perni M, Bertoldo JB, Bernardim B, Peter Q, Jiménez-Osés G, Knowles TPJ, Dobson CM, Corzana F, Vendruscolo M, Bernardes GJL.

ACS Cent Sci. 2019 Aug 28;5(8):1417-1424. doi: 10.1021/acscentsci.9b00467. Epub 2019 Jul 19.


Soluble aggregates present in cerebrospinal fluid change in size and mechanism of toxicity during Alzheimer's disease progression.

De S, Whiten DR, Ruggeri FS, Hughes C, Rodrigues M, Sideris DI, Taylor CG, Aprile FA, Muyldermans S, Knowles TPJ, Vendruscolo M, Bryant C, Blennow K, Skoog I, Kern S, Zetterberg H, Klenerman D.

Acta Neuropathol Commun. 2019 Jul 26;7(1):120. doi: 10.1186/s40478-019-0777-4.


Different soluble aggregates of Aβ42 can give rise to cellular toxicity through different mechanisms.

De S, Wirthensohn DC, Flagmeier P, Hughes C, Aprile FA, Ruggeri FS, Whiten DR, Emin D, Xia Z, Varela JA, Sormanni P, Kundel F, Knowles TPJ, Dobson CM, Bryant C, Vendruscolo M, Klenerman D.

Nat Commun. 2019 Apr 4;10(1):1541. doi: 10.1038/s41467-019-09477-3.


Automated Behavioral Analysis of Large C. elegans Populations Using a Wide Field-of-view Tracking Platform.

Perni M, Casford S, Aprile FA, Nollen EA, Knowles TPJ, Vendruscolo M, Dobson CM.

J Vis Exp. 2018 Nov 28;(141). doi: 10.3791/58643.


Third generation antibody discovery methods: in silico rational design.

Sormanni P, Aprile FA, Vendruscolo M.

Chem Soc Rev. 2018 Dec 10;47(24):9137-9157. doi: 10.1039/c8cs00523k. Review.


Targeting Amyloid Aggregation: An Overview of Strategies and Mechanisms.

Giorgetti S, Greco C, Tortora P, Aprile FA.

Int J Mol Sci. 2018 Sep 9;19(9). pii: E2677. doi: 10.3390/ijms19092677. Review.


Author Correction: The molecular chaperones DNAJB6 and Hsp70 cooperate to suppress α-synuclein aggregation.

Aprile FA, Källstig E, Limorenko G, Vendruscolo M, Ron D, Hansen C.

Sci Rep. 2018 Aug 2;8(1):11848. doi: 10.1038/s41598-018-29893-7.


Multistep Inhibition of α-Synuclein Aggregation and Toxicity in Vitro and in Vivo by Trodusquemine.

Perni M, Flagmeier P, Limbocker R, Cascella R, Aprile FA, Galvagnion C, Heller GT, Meisl G, Chen SW, Kumita JR, Challa PK, Kirkegaard JB, Cohen SIA, Mannini B, Barbut D, Nollen EAA, Cecchi C, Cremades N, Knowles TPJ, Chiti F, Zasloff M, Vendruscolo M, Dobson CM.

ACS Chem Biol. 2018 Aug 17;13(8):2308-2319. doi: 10.1021/acschembio.8b00466. Epub 2018 Jun 28.


Cooperative Assembly of Hsp70 Subdomain Clusters.

Wright MA, Aprile FA, Bellaiche MMJ, Michaels TCT, Müller T, Arosio P, Vendruscolo M, Dobson CM, Knowles TPJ.

Biochemistry. 2018 Jul 3;57(26):3641-3649. doi: 10.1021/acs.biochem.8b00151. Epub 2018 May 29.


A Rationally Designed Hsp70 Variant Rescues the Aggregation-Associated Toxicity of Human IAPP in Cultured Pancreatic Islet β-Cells.

Bongiovanni MN, Aprile FA, Sormanni P, Vendruscolo M.

Int J Mol Sci. 2018 May 12;19(5). pii: E1443. doi: 10.3390/ijms19051443.


Publisher Correction: The polyglutamine protein ataxin-3 enables normal growth under heat shock conditions in the methylotrophic yeast Pichia pastoris.

Bonanomi M, Roffia V, De Palma A, Lombardi A, Aprile FA, Visentin C, Tortora P, Mauri P, Regonesi ME.

Sci Rep. 2018 Mar 6;8(1):4285. doi: 10.1038/s41598-018-22523-2.


Delivery of Native Proteins into C. elegans Using a Transduction Protocol Based on Lipid Vesicles.

Perni M, Aprile FA, Casford S, Mannini B, Sormanni P, Dobson CM, Vendruscolo M.

Sci Rep. 2017 Nov 8;7(1):15045. doi: 10.1038/s41598-017-13755-9.


A Water-Bridged Cysteine-Cysteine Redox Regulation Mechanism in Bacterial Protein Tyrosine Phosphatases.

Bertoldo JB, Rodrigues T, Dunsmore L, Aprile FA, Marques MC, Rosado LA, Boutureira O, Steinbrecher TB, Sherman W, Corzana F, Terenzi H, Bernardes GJL.

Chem. 2017 Oct 12;3(4):665-677. doi: 10.1016/j.chempr.2017.07.009.


The polyglutamine protein ataxin-3 enables normal growth under heat shock conditions in the methylotrophic yeast Pichia pastoris.

Bonanomi M, Roffia V, De Palma A, Lombardi A, Aprile FA, Visentin C, Tortora P, Mauri P, Regonesi ME.

Sci Rep. 2017 Oct 17;7(1):13417. doi: 10.1038/s41598-017-13814-1. Erratum in: Sci Rep. 2018 Mar 6;8(1):4285.


The molecular chaperones DNAJB6 and Hsp70 cooperate to suppress α-synuclein aggregation.

Aprile FA, Källstig E, Limorenko G, Vendruscolo M, Ron D, Hansen C.

Sci Rep. 2017 Aug 22;7(1):9039. doi: 10.1038/s41598-017-08324-z. Erratum in: Sci Rep. 2018 Aug 2;8(1):11848.


Sequence Specificity in the Entropy-Driven Binding of a Small Molecule and a Disordered Peptide.

Heller GT, Aprile FA, Bonomi M, Camilloni C, De Simone A, Vendruscolo M.

J Mol Biol. 2017 Sep 1;429(18):2772-2779. doi: 10.1016/j.jmb.2017.07.016. Epub 2017 Jul 22.


Selective targeting of primary and secondary nucleation pathways in Aβ42 aggregation using a rational antibody scanning method.

Aprile FA, Sormanni P, Perni M, Arosio P, Linse S, Knowles TPJ, Dobson CM, Vendruscolo M.

Sci Adv. 2017 Jun 21;3(6):e1700488. doi: 10.1126/sciadv.1700488. eCollection 2017 Jun.


Methods of probing the interactions between small molecules and disordered proteins.

Heller GT, Aprile FA, Vendruscolo M.

Cell Mol Life Sci. 2017 Sep;74(17):3225-3243. doi: 10.1007/s00018-017-2563-4. Epub 2017 Jun 19. Review.


Identification of an RNA Polymerase III Regulator Linked to Disease-Associated Protein Aggregation.

Sin O, de Jong T, Mata-Cabana A, Kudron M, Zaini MA, Aprile FA, Seinstra RI, Stroo E, Prins RW, Martineau CN, Wang HH, Hogewerf W, Steinhof A, Wanker EE, Vendruscolo M, Calkhoven CF, Reinke V, Guryev V, Nollen EA.

Mol Cell. 2017 Mar 16;65(6):1096-1108.e6. doi: 10.1016/j.molcel.2017.02.022.


Inhibition of α-Synuclein Fibril Elongation by Hsp70 Is Governed by a Kinetic Binding Competition between α-Synuclein Species.

Aprile FA, Arosio P, Fusco G, Chen SW, Kumita JR, Dhulesia A, Tortora P, Knowles TP, Vendruscolo M, Dobson CM, Cremades N.

Biochemistry. 2017 Mar 7;56(9):1177-1180. doi: 10.1021/acs.biochem.6b01178. Epub 2017 Feb 23.


A natural product inhibits the initiation of α-synuclein aggregation and suppresses its toxicity.

Perni M, Galvagnion C, Maltsev A, Meisl G, Müller MB, Challa PK, Kirkegaard JB, Flagmeier P, Cohen SI, Cascella R, Chen SW, Limbocker R, Sormanni P, Heller GT, Aprile FA, Cremades N, Cecchi C, Chiti F, Nollen EA, Knowles TP, Vendruscolo M, Bax A, Zasloff M, Dobson CM.

Proc Natl Acad Sci U S A. 2017 Feb 7;114(6):E1009-E1017. doi: 10.1073/pnas.1610586114. Epub 2017 Jan 17. Erratum in: Proc Natl Acad Sci U S A. 2017 Mar 21;114(12 ):E2543. Limboker, Ryan [corrected to Limbocker, Ryan].


Structure of a low-population binding intermediate in protein-RNA recognition.

Borkar AN, Bardaro MF Jr, Camilloni C, Aprile FA, Varani G, Vendruscolo M.

Proc Natl Acad Sci U S A. 2016 Jun 28;113(26):7171-6. doi: 10.1073/pnas.1521349113. Epub 2016 Jun 10.


Microfluidic Diffusion Viscometer for Rapid Analysis of Complex Solutions.

Arosio P, Hu K, Aprile FA, Müller T, Knowles TP.

Anal Chem. 2016 Apr 5;88(7):3488-93. doi: 10.1021/acs.analchem.5b02930. Epub 2016 Mar 16.


Microfluidic Diffusion Analysis of the Sizes and Interactions of Proteins under Native Solution Conditions.

Arosio P, Müller T, Rajah L, Yates EV, Aprile FA, Zhang Y, Cohen SI, White DA, Herling TW, De Genst EJ, Linse S, Vendruscolo M, Dobson CM, Knowles TP.

ACS Nano. 2016 Jan 26;10(1):333-41. doi: 10.1021/acsnano.5b04713. Epub 2015 Dec 23.


Biophysical approaches for the study of interactions between molecular chaperones and protein aggregates.

Wright MA, Aprile FA, Arosio P, Vendruscolo M, Dobson CM, Knowles TP.

Chem Commun (Camb). 2015 Oct 4;51(77):14425-34. doi: 10.1039/c5cc03689e. Review.


Cell surface localised Hsp70 is a cancer specific regulator of clathrin-independent endocytosis.

Nimmervoll B, Chtcheglova LA, Juhasz K, Cremades N, Aprile FA, Sonnleitner A, Hinterdorfer P, Vigh L, Preiner J, Balogi Z.

FEBS Lett. 2015 Sep 14;589(19 Pt B):2747-53. doi: 10.1016/j.febslet.2015.07.037. Epub 2015 Aug 6.


Rational design of antibodies targeting specific epitopes within intrinsically disordered proteins.

Sormanni P, Aprile FA, Vendruscolo M.

Proc Natl Acad Sci U S A. 2015 Aug 11;112(32):9902-7. doi: 10.1073/pnas.1422401112. Epub 2015 Jul 27.


A Rational Design Strategy for the Selective Activity Enhancement of a Molecular Chaperone toward a Target Substrate.

Aprile FA, Sormanni P, Vendruscolo M.

Biochemistry. 2015 Aug 18;54(32):5103-12. doi: 10.1021/acs.biochem.5b00459. Epub 2015 Aug 7.


Structural characterization of toxic oligomers that are kinetically trapped during α-synuclein fibril formation.

Chen SW, Drakulic S, Deas E, Ouberai M, Aprile FA, Arranz R, Ness S, Roodveldt C, Guilliams T, De-Genst EJ, Klenerman D, Wood NW, Knowles TP, Alfonso C, Rivas G, Abramov AY, Valpuesta JM, Dobson CM, Cremades N.

Proc Natl Acad Sci U S A. 2015 Apr 21;112(16):E1994-2003. doi: 10.1073/pnas.1421204112. Epub 2015 Apr 8.


Structure and dynamics of the integrin LFA-1 I-domain in the inactive state underlie its inside-out/outside-in signaling and allosteric mechanisms.

Kukic P, Alvin Leung HT, Bemporad F, Aprile FA, Kumita JR, De Simone A, Camilloni C, Vendruscolo M.

Structure. 2015 Apr 7;23(4):745-53. doi: 10.1016/j.str.2014.12.020. Epub 2015 Mar 12.


Structure of a low-population intermediate state in the release of an enzyme product.

De Simone A, Aprile FA, Dhulesia A, Dobson CM, Vendruscolo M.

Elife. 2015 Jan 9;4. doi: 10.7554/eLife.02777.


The CamSol method of rational design of protein mutants with enhanced solubility.

Sormanni P, Aprile FA, Vendruscolo M.

J Mol Biol. 2015 Jan 30;427(2):478-90. doi: 10.1016/j.jmb.2014.09.026. Epub 2014 Oct 14.


NMR characterization of the conformational fluctuations of the human lymphocyte function-associated antigen-1 I-domain.

Leung HT, Kukic P, Camilloni C, Bemporad F, De Simone A, Aprile FA, Kumita JR, Vendruscolo M.

Protein Sci. 2014 Nov;23(11):1596-606. doi: 10.1002/pro.2538. Epub 2014 Sep 5.


Hsp70 oligomerization is mediated by an interaction between the interdomain linker and the substrate-binding domain.

Aprile FA, Dhulesia A, Stengel F, Roodveldt C, Benesch JL, Tortora P, Robinson CV, Salvatella X, Dobson CM, Cremades N.

PLoS One. 2013 Jun 28;8(6):e67961. doi: 10.1371/journal.pone.0067961. Print 2013.


Nanobodies raised against monomeric α-synuclein distinguish between fibrils at different maturation stages.

Guilliams T, El-Turk F, Buell AK, O'Day EM, Aprile FA, Esbjörner EK, Vendruscolo M, Cremades N, Pardon E, Wyns L, Welland ME, Steyaert J, Christodoulou J, Dobson CM, De Genst E.

J Mol Biol. 2013 Jul 24;425(14):2397-411. doi: 10.1016/j.jmb.2013.01.040. Epub 2013 Apr 1.


The relationship between aggregation and toxicity of polyglutamine-containing ataxin-3 in the intracellular environment of Escherichia coli.

Invernizzi G, Aprile FA, Natalello A, Ghisleni A, Penco A, Relini A, Doglia SM, Tortora P, Regonesi ME.

PLoS One. 2012;7(12):e51890. doi: 10.1371/journal.pone.0051890. Epub 2012 Dec 14.


Direct observation of the interconversion of normal and toxic forms of α-synuclein.

Cremades N, Cohen SI, Deas E, Abramov AY, Chen AY, Orte A, Sandal M, Clarke RW, Dunne P, Aprile FA, Bertoncini CW, Wood NW, Knowles TP, Dobson CM, Klenerman D.

Cell. 2012 May 25;149(5):1048-59. doi: 10.1016/j.cell.2012.03.037.

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