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Items: 11


Exploring protein-DNA interactions in 3D using in situ construction, manipulation and visualization of individual DNA dumbbells with optical traps, microfluidics and fluorescence microscopy.

Forget AL, Dombrowski CC, Amitani I, Kowalczykowski SC.

Nat Protoc. 2013 Mar;8(3):525-38. doi: 10.1038/nprot.2013.016. Epub 2013 Feb 14.


Watching individual proteins acting on single molecules of DNA.

Amitani I, Liu B, Dombrowski CC, Baskin RJ, Kowalczykowski SC.

Methods Enzymol. 2010;472:261-91. doi: 10.1016/S0076-6879(10)72007-3.


Single molecule analysis of a red fluorescent RecA protein reveals a defect in nucleoprotein filament nucleation that relates to its reduced biological functions.

Handa N, Amitani I, Gumlaw N, Sandler SJ, Kowalczykowski SC.

J Biol Chem. 2009 Jul 10;284(28):18664-73. doi: 10.1074/jbc.M109.004895. Epub 2009 May 5.


The BRC repeats of BRCA2 modulate the DNA-binding selectivity of RAD51.

Carreira A, Hilario J, Amitani I, Baskin RJ, Shivji MK, Venkitaraman AR, Kowalczykowski SC.

Cell. 2009 Mar 20;136(6):1032-43. doi: 10.1016/j.cell.2009.02.019.


Direct imaging of human Rad51 nucleoprotein dynamics on individual DNA molecules.

Hilario J, Amitani I, Baskin RJ, Kowalczykowski SC.

Proc Natl Acad Sci U S A. 2009 Jan 13;106(2):361-8. doi: 10.1073/pnas.0811965106. Epub 2009 Jan 2.


RecBCD enzyme switches lead motor subunits in response to chi recognition.

Spies M, Amitani I, Baskin RJ, Kowalczykowski SC.

Cell. 2007 Nov 16;131(4):694-705.


Single molecule imaging of Tid1/Rdh54, a Rad54 homolog that translocates on duplex DNA and can disrupt joint molecules.

Nimonkar AV, Amitani I, Baskin RJ, Kowalczykowski SC.

J Biol Chem. 2007 Oct 19;282(42):30776-84. Epub 2007 Aug 16.


Direct observation of individual RecA filaments assembling on single DNA molecules.

Galletto R, Amitani I, Baskin RJ, Kowalczykowski SC.

Nature. 2006 Oct 19;443(7113):875-8. Epub 2006 Sep 20.


Visualization of Rad54, a chromatin remodeling protein, translocating on single DNA molecules.

Amitani I, Baskin RJ, Kowalczykowski SC.

Mol Cell. 2006 Jul 7;23(1):143-8.


Link between the enzymatic kinetics and mechanical behavior in an actomyosin motor.

Amitani I, Sakamoto T, Ando T.

Biophys J. 2001 Jan;80(1):379-97.


Direct observation of processive movement by individual myosin V molecules.

Sakamoto T, Amitani I, Yokota E, Ando T.

Biochem Biophys Res Commun. 2000 Jun 7;272(2):586-90.


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