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Items: 1 to 50 of 80

1.

Classical Genetics with Saccharomyces cerevisiae.

Amberg DC, Burke DJ.

Cold Spring Harb Protoc. 2016 May 2;2016(5). doi: 10.1101/pdb.top077628.

PMID:
27140921
2.

The Gcn2 Regulator Yih1 Interacts with the Cyclin Dependent Kinase Cdc28 and Promotes Cell Cycle Progression through G2/M in Budding Yeast.

Silva RC, Dautel M, Di Genova BM, Amberg DC, Castilho BA, Sattlegger E.

PLoS One. 2015 Jul 15;10(7):e0131070. doi: 10.1371/journal.pone.0131070. eCollection 2015.

3.

Using two-hybrid interactions to identify separation-of-function mutations.

Haarer B, Amberg DC.

Methods Mol Biol. 2014;1205:131-42. doi: 10.1007/978-1-4939-1363-3_9.

PMID:
25213243
4.

Coordination of the filament stabilizing versus destabilizing activities of cofilin through its secondary binding site on actin.

Aggeli D, Kish-Trier E, Lin MC, Haarer B, Cingolani G, Cooper JA, Wilkens S, Amberg DC.

Cytoskeleton (Hoboken). 2014 Jun;71(6):361-79. doi: 10.1002/cm.21178. Epub 2014 Jun 23.

5.

Linking genetics to structural biology: complex heterozygosity screening with actin alanine scan alleles identifies functionally related surfaces on yeast actin.

DiPrima S, Haarer B, Viggiano S, Pons C, Myers CL, Amberg DC.

G3 (Bethesda). 2014 Jun 17;4(8):1491-501. doi: 10.1534/g3.114.012054.

6.

Novel interactions between actin and the proteasome revealed by complex haploinsufficiency.

Haarer B, Aggeli D, Viggiano S, Burke DJ, Amberg DC.

PLoS Genet. 2011 Sep;7(9):e1002288. doi: 10.1371/journal.pgen.1002288. Epub 2011 Sep 22.

7.

Diverse protective roles of the actin cytoskeleton during oxidative stress.

Farah ME, Sirotkin V, Haarer B, Kakhniashvili D, Amberg DC.

Cytoskeleton (Hoboken). 2011 Jun;68(6):340-54. doi: 10.1002/cm.20516. Epub 2011 Jun 10.

8.

Correction/completion of the yeast actin, alanine scan alleles.

Viggiano S, Haarer B, Amberg DC.

Genetics. 2010 May;185(1):391-4. doi: 10.1534/genetics.110.114942. Epub 2010 Mar 9. No abstract available.

9.

Synthetic genetic array analysis in Saccharomyces cerevisiae provides evidence for an interaction between RAT8/DBP5 and genes encoding P-body components.

Scarcelli JJ, Viggiano S, Hodge CA, Heath CV, Amberg DC, Cole CN.

Genetics. 2008 Aug;179(4):1945-55. doi: 10.1534/genetics.108.091256. Epub 2008 Aug 9.

11.

Stable preanaphase spindle positioning requires Bud6p and an apparent interaction between the spindle pole bodies and the neck.

Haarer BK, Helfant AH, Nelson SA, Cooper JA, Amberg DC.

Eukaryot Cell. 2007 May;6(5):797-807. Epub 2007 Apr 6.

12.

The MEK kinases MEKK4/Ssk2p facilitate complexity in the stress signaling responses of diverse systems.

Bettinger BT, Amberg DC.

J Cell Biochem. 2007 May 1;101(1):34-43. Review.

PMID:
17348032
13.

Conserved actin cysteine residues are oxidative stress sensors that can regulate cell death in yeast.

Farah ME, Amberg DC.

Mol Biol Cell. 2007 Apr;18(4):1359-65. Epub 2007 Feb 7. Erratum in: Mol Biol Cell. 2010 Mar;21(5):843.

14.
15.

Modeling complex genetic interactions in a simple eukaryotic genome: actin displays a rich spectrum of complex haploinsufficiencies.

Haarer B, Viggiano S, Hibbs MA, Troyanskaya OG, Amberg DC.

Genes Dev. 2007 Jan 15;21(2):148-59. Epub 2006 Dec 13.

16.

A genetic dissection of Aip1p's interactions leads to a model for Aip1p-cofilin cooperative activities.

Clark MG, Teply J, Haarer BK, Viggiano SC, Sept D, Amberg DC.

Mol Biol Cell. 2006 Apr;17(4):1971-84. Epub 2006 Jan 18.

17.

Measuring yeast cell density by spectrophotometry.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4186. doi: 10.1101/pdb.prot4186. No abstract available.

PMID:
22485587
18.

Determining plating efficiency in yeast: direct method.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4185. doi: 10.1101/pdb.prot4185. No abstract available.

PMID:
22485586
19.

Determining plating efficiency in yeast: indirect method.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4184. doi: 10.1101/pdb.prot4184. No abstract available.

PMID:
22485585
20.

Picking zygotes.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4183. doi: 10.1101/pdb.prot4183. No abstract available.

PMID:
22485584
21.

Making a tetrad dissection needle.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4182. doi: 10.1101/pdb.prot4182. No abstract available.

PMID:
22485583
22.

Tetrad dissection.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4181. doi: 10.1101/pdb.prot4181. No abstract available.

PMID:
22485582
23.

Ethyl Methane Sulfonate (EMS) Mutagenesis.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4180. doi: 10.1101/pdb.prot4180. No abstract available.

PMID:
22485581
24.

Logarithmic growth.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4179. doi: 10.1101/pdb.prot4179. No abstract available.

PMID:
22485580
25.

Preparation of yeast cells for flow cytometry.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4178. doi: 10.1101/pdb.prot4178. No abstract available.

PMID:
22485579
26.

Chromatin immunoprecipitation in yeast.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4177. doi: 10.1101/pdb.prot4177. No abstract available.

PMID:
22485578
27.

Inducing yeast cell synchrony: dilution of stationary-phase cultures.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4176. doi: 10.1101/pdb.prot4176. No abstract available.

PMID:
22485577
28.

Inducing yeast cell synchrony: nocodazole arrest.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4175. doi: 10.1101/pdb.prot4175. No abstract available.

PMID:
22485576
29.

Inducing yeast cell synchrony: hydroxyurea arrest.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4174. doi: 10.1101/pdb.prot4174. No abstract available.

PMID:
22485575
30.

Inducing Yeast Cell Synchrony: α-Factor Arrest Using bar1 Mutants.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4173. doi: 10.1101/pdb.prot4173. No abstract available.

PMID:
22485574
31.

Inducing Yeast Cell Synchrony: α-Factor Arrest Using Low pH.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4172. doi: 10.1101/pdb.prot4172. No abstract available.

PMID:
22485573
32.

Inducing Yeast Cell Synchrony: α-Factor Arrest Using Low Cell Concentration.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4171. doi: 10.1101/pdb.prot4171. No abstract available.

PMID:
22485572
33.

Yeast colony PCR.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4170. doi: 10.1101/pdb.prot4170. No abstract available.

PMID:
22485571
34.

PCR-Mediated Gene Disruption: One-Step Method.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4169. doi: 10.1101/pdb.prot4169. No abstract available.

PMID:
22485570
35.

Actin staining in fixed yeast cells.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4168. doi: 10.1101/pdb.prot4168. No abstract available.

PMID:
22485569
36.

Yeast immunofluorescence.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4167. doi: 10.1101/pdb.prot4167. No abstract available.

PMID:
22485568
37.

Yeast vital stains: calcofluor staining of chitin and bud scars.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4166. doi: 10.1101/pdb.prot4166. No abstract available.

PMID:
22485567
38.

Yeast Vital Stains: Visualizing Vacuoles and Endocytic Compartments with FM4-64.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4165. doi: 10.1101/pdb.prot4165. No abstract available.

PMID:
22485566
39.

Yeast Vital Stains: Visualizing Mitochondria with DiOC6 or DiIC5(3).

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4164. doi: 10.1101/pdb.prot4164. No abstract available.

PMID:
22485565
40.

Yeast Vital Stains: DAPI Stain of Nuclear and Mitochondrial DNA.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4163. doi: 10.1101/pdb.prot4163. No abstract available.

PMID:
22485564
41.

Random spore analysis in yeast.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4162. doi: 10.1101/pdb.prot4162. No abstract available.

PMID:
22485563
42.

Plate assay for carboxypeptidase y in yeast cells.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4161. doi: 10.1101/pdb.prot4161. No abstract available.

PMID:
22485562
43.

Assay of β-Galactosidase in Yeast: Freeze/Thaw Assay by Chemiluminescence in 96-Well Plates.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4160. doi: 10.1101/pdb.prot4160. No abstract available.

PMID:
22485561
44.

Assay of β-Galactosidase in Yeast: Freeze/Thaw Assay by Chemiluminescence in Microcentrifuge Tubes.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4159. doi: 10.1101/pdb.prot4159. No abstract available.

PMID:
22485560
45.

Assay of β-Galactosidase in Yeast: Permeabilized Cell Assay.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4158. doi: 10.1101/pdb.prot4158. No abstract available.

PMID:
22485559
46.

Assay of β-Galactosidase in Yeast: Assay of Crude Extracts.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4157. doi: 10.1101/pdb.prot4157. No abstract available.

PMID:
22485558
47.

Hydroxylamine mutagenesis of plasmid DNA.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4156. doi: 10.1101/pdb.prot4156. No abstract available.

PMID:
22485557
48.

Yeast RNA isolation: small-scale.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4155. doi: 10.1101/pdb.prot4155. No abstract available.

PMID:
22485556
49.

Yeast RNA isolation: large-scale.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4154. doi: 10.1101/pdb.prot4154. No abstract available.

PMID:
22485555
50.

Tandem Affinity Protein (TAP) Purification from Yeast.

Amberg DC, Burke DJ, Strathern JN.

CSH Protoc. 2006 Jun 1;2006(1). pii: pdb.prot4153. doi: 10.1101/pdb.prot4153. No abstract available.

PMID:
22485554

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