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Clin Vaccine Immunol. 2007 Feb;14(2):201-3. Epub 2006 Dec 13.

Evaluation of dried whole blood spots obtained by heel or finger stick as an alternative to venous blood for diagnosis of human immunodeficiency virus type 1 infection in vertically exposed infants in the routine diagnostic laboratory.

Author information

1
Wits Pediatric HIV Clinics, Wits Health Consortium, University of the Witwatersrand, Johannesburg, Gauteng, South Africa.

Abstract

The diagnostic accuracy of the Roche Amplicor human immunodeficiency virus type 1 DNA PCR assay (version 1.5) on DNA extracted from pediatric heel prick dried blood spots using Roche MagNA Pure nucleic acid purification technology was evaluated. The methodologies transfer successfully from the labor-intensive research laboratory to the high-throughput automated routine laboratory.

PMID:
17167036
PMCID:
PMC1797794
DOI:
10.1128/CVI.00223-06
[Indexed for MEDLINE]
Free PMC Article

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