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Items: 1 to 50 of 65

1.

YHp as a highly stable, hyper-copy, hyper-expression plasmid constructed using a full 2-μm circle sequence in cir0 strains of Saccharomyces cerevisiae.

Misumi Y, Nishioka S, Fukuda A, Uemura T, Nakamura M, Hoshida H, Akada R.

Yeast. 2019 May;36(5):249-257. doi: 10.1002/yea.3371. Epub 2019 Jan 24.

PMID:
30537227
2.
3.

Enhanced production of extracellular inulinase by the yeast Kluyveromyces marxianus in xylose catabolic state.

Hoshida H, Kidera K, Takishita R, Fujioka N, Fukagawa T, Akada R.

J Biosci Bioeng. 2018 Jun;125(6):676-681. doi: 10.1016/j.jbiosc.2017.12.024. Epub 2018 Jan 20.

PMID:
29370981
4.

Enzymatic Hydrolysis of Black Liquor Xylan by a Novel Xylose-Tolerant, Thermostable β-Xylosidase from a Tropical Strain of Aureobasidium pullulans CBS 135684.

Bankeeree W, Akada R, Lotrakul P, Punnapayak H, Prasongsuk S.

Appl Biochem Biotechnol. 2018 Mar;184(3):919-934. doi: 10.1007/s12010-017-2598-x. Epub 2017 Sep 16.

PMID:
28918541
5.

Polymorphisms in the LAC12 gene explain lactose utilisation variability in Kluyveromyces marxianus strains.

Varela JA, Montini N, Scully D, Van der Ploeg R, Oreb M, Boles E, Hirota J, Akada R, Hoshida H, Morrissey JP.

FEMS Yeast Res. 2017 May 1;17(3). doi: 10.1093/femsyr/fox021.

PMID:
28444380
6.

5´-UTR introns enhance protein expression in the yeast Saccharomyces cerevisiae.

Hoshida H, Kondo M, Kobayashi T, Yarimizu T, Akada R.

Appl Microbiol Biotechnol. 2017 Jan;101(1):241-251. doi: 10.1007/s00253-016-7891-z. Epub 2016 Oct 12.

PMID:
27734122
7.

Selection of Error-Less Synthetic Genes in Yeast.

Hoshida H, Yarimizu T, Akada R.

Methods Mol Biol. 2017;1472:237-46. doi: 10.1007/978-1-4939-6343-0_18.

PMID:
27671945
8.

Genomic analyses of thermotolerant microorganisms used for high-temperature fermentations.

Matsushita K, Azuma Y, Kosaka T, Yakushi T, Hoshida H, Akada R, Yamada M.

Biosci Biotechnol Biochem. 2016;80(4):655-68. doi: 10.1080/09168451.2015.1104235. Epub 2015 Nov 13. Review.

PMID:
26566045
9.

The Thermotolerant Yeast Kluyveromyces marxianus Is a Useful Organism for Structural and Biochemical Studies of Autophagy.

Yamamoto H, Shima T, Yamaguchi M, Mochizuki Y, Hoshida H, Kakuta S, Kondo-Kakuta C, Noda NN, Inagaki F, Itoh T, Akada R, Ohsumi Y.

J Biol Chem. 2015 Dec 4;290(49):29506-18. doi: 10.1074/jbc.M115.684233. Epub 2015 Oct 6.

10.

End Joining-Mediated Gene Expression in Mammalian Cells Using PCR-Amplified DNA Constructs that Contain Terminator in Front of Promoter.

Nakamura M, Suzuki A, Akada J, Tomiyoshi K, Hoshida H, Akada R.

Mol Biotechnol. 2015 Dec;57(11-12):1018-29. doi: 10.1007/s12033-015-9890-1.

PMID:
26350674
11.

Gene expression analysis using strains constructed by NHEJ-mediated one-step promoter cloning in the yeast Kluyveromyces marxianus.

Suzuki A, Fujii H, Hoshida H, Akada R.

FEMS Yeast Res. 2015 Sep;15(6). pii: fov059. doi: 10.1093/femsyr/fov059. Epub 2015 Jun 30.

PMID:
26136515
12.

A Novel Terminator Primer and Enhancer Reagents for Direct Expression of PCR-Amplified Genes in Mammalian Cells.

Nakamura M, Suzuki A, Akada J, Yarimizu T, Iwakiri R, Hoshida H, Akada R.

Mol Biotechnol. 2015 Aug;57(8):767-80. doi: 10.1007/s12033-015-9870-5.

PMID:
25997599
13.

Mutant screening for oncogenes of Ewing's sarcoma using yeast.

Kitagawa T, Okita H, Baron B, Tokuda K, Nakamura M, Wang Y, Akada J, Hoshida H, Akada R, Kuramitsu Y, Nakamura K.

Appl Microbiol Biotechnol. 2015 Aug;99(16):6737-44. doi: 10.1007/s00253-015-6621-2. Epub 2015 May 5.

PMID:
25936378
14.

Synthetic signal sequences that enable efficient secretory protein production in the yeast Kluyveromyces marxianus.

Yarimizu T, Nakamura M, Hoshida H, Akada R.

Microb Cell Fact. 2015 Feb 14;14:20. doi: 10.1186/s12934-015-0203-y.

15.

Screening of accurate clones for gene synthesis in yeast.

Yarimizu T, Nakamura M, Hoshida H, Akada R.

J Biosci Bioeng. 2015 Mar;119(3):251-9. doi: 10.1016/j.jbiosc.2014.08.006. Epub 2014 Sep 6.

PMID:
25201012
16.

Structural basis of starvation-induced assembly of the autophagy initiation complex.

Fujioka Y, Suzuki SW, Yamamoto H, Kondo-Kakuta C, Kimura Y, Hirano H, Akada R, Inagaki F, Ohsumi Y, Noda NN.

Nat Struct Mol Biol. 2014 Jun;21(6):513-21. doi: 10.1038/nsmb.2822. Epub 2014 May 4.

PMID:
24793651
17.

Minimum GC-rich sequences for overlap extension PCR and primer annealing.

Nakamura M, Suzuki A, Hoshida H, Akada R.

Methods Mol Biol. 2014;1116:165-81. doi: 10.1007/978-1-62703-764-8_12.

PMID:
24395364
18.

Non-homologous end joining-mediated functional marker selection for DNA cloning in the yeast Kluyveromyces marxianus.

Hoshida H, Murakami N, Suzuki A, Tamura R, Asakawa J, Abdel-Banat BM, Nonklang S, Nakamura M, Akada R.

Yeast. 2014 Jan;31(1):29-46. doi: 10.1002/yea.2993. Epub 2013 Dec 19.

19.

Identification of auxotrophic mutants of the yeast Kluyveromyces marxianus by non-homologous end joining-mediated integrative transformation with genes from Saccharomyces cerevisiae.

Yarimizu T, Nonklang S, Nakamura J, Tokuda S, Nakagawa T, Lorreungsil S, Sutthikhumpha S, Pukahuta C, Kitagawa T, Nakamura M, Cha-Aim K, Limtong S, Hoshida H, Akada R.

Yeast. 2013 Dec;30(12):485-500. doi: 10.1002/yea.2985. Epub 2013 Nov 19.

20.

Novel small-molecule compounds that affect cellular morphogenesis in yeast and mammalian cells.

Fukunaga T, Nakamura M, Kitagawa T, Watanapokasin R, Hoshida H, Akada R.

Biosci Biotechnol Biochem. 2013;77(8):1669-76. Epub 2013 Aug 7.

21.

Designed construction of recombinant DNA at the ura3Δ0 locus in the yeast Saccharomyces cerevisiae.

Fukunaga T, Cha-Aim K, Hirakawa Y, Sakai R, Kitagawa T, Nakamura M, Nonklang S, Hoshida H, Akada R.

Yeast. 2013 Jun;30(6):243-53. doi: 10.1002/yea.2957. Epub 2013 May 20.

22.

N-Glycosylation deficiency enhanced heterologous production of a Bacillus licheniformis thermostable α-amylase in Saccharomyces cerevisiae.

Hoshida H, Fujita T, Cha-aim K, Akada R.

Appl Microbiol Biotechnol. 2013 Jun;97(12):5473-82. doi: 10.1007/s00253-012-4582-2. Epub 2013 Jan 11.

PMID:
23306636
23.

Noncanonical recognition and UBL loading of distinct E2s by autophagy-essential Atg7.

Yamaguchi M, Matoba K, Sawada R, Fujioka Y, Nakatogawa H, Yamamoto H, Kobashigawa Y, Hoshida H, Akada R, Ohsumi Y, Noda NN, Inagaki F.

Nat Struct Mol Biol. 2012 Dec;19(12):1250-6. doi: 10.1038/nsmb.2451. Epub 2012 Nov 11.

PMID:
23142983
24.

Structure-based analyses reveal distinct binding sites for Atg2 and phosphoinositides in Atg18.

Watanabe Y, Kobayashi T, Yamamoto H, Hoshida H, Akada R, Inagaki F, Ohsumi Y, Noda NN.

J Biol Chem. 2012 Sep 14;287(38):31681-90. doi: 10.1074/jbc.M112.397570. Epub 2012 Jul 31.

25.

Structural insights into Atg10-mediated formation of the autophagy-essential Atg12-Atg5 conjugate.

Yamaguchi M, Noda NN, Yamamoto H, Shima T, Kumeta H, Kobashigawa Y, Akada R, Ohsumi Y, Inagaki F.

Structure. 2012 Jul 3;20(7):1244-54. doi: 10.1016/j.str.2012.04.018. Epub 2012 Jun 7.

26.

Construction of a URA3 deletion strain from the allotetraploid bottom-fermenting yeast Saccharomyces pastorianus.

Murakami N, Miyoshi S, Yokoyama R, Hoshida H, Akada R, Ogata T.

Yeast. 2012 May;29(5):155-65. doi: 10.1002/yea.2897. Epub 2012 May 11.

27.

Fusion PCR via novel overlap sequences.

Cha-Aim K, Hoshida H, Fukunaga T, Akada R.

Methods Mol Biol. 2012;852:97-110. doi: 10.1007/978-1-61779-564-0_8.

PMID:
22328428
28.

Whole-genome sequencing of sake yeast Saccharomyces cerevisiae Kyokai no. 7.

Akao T, Yashiro I, Hosoyama A, Kitagaki H, Horikawa H, Watanabe D, Akada R, Ando Y, Harashima S, Inoue T, Inoue Y, Kajiwara S, Kitamoto K, Kitamoto N, Kobayashi O, Kuhara S, Masubuchi T, Mizoguchi H, Nakao Y, Nakazato A, Namise M, Oba T, Ogata T, Ohta A, Sato M, Shibasaki S, Takatsume Y, Tanimoto S, Tsuboi H, Nishimura A, Yoda K, Ishikawa T, Iwashita K, Fujita N, Shimoi H.

DNA Res. 2011 Dec;18(6):423-34. doi: 10.1093/dnares/dsr029. Epub 2011 Sep 6.

29.

Identification of genes that enhance cellulase protein production in yeast.

Kitagawa T, Kohda K, Tokuhiro K, Hoshida H, Akada R, Takahashi H, Imaeda T.

J Biotechnol. 2011 Jan 20;151(2):194-203. doi: 10.1016/j.jbiotec.2010.12.002. Epub 2010 Dec 15.

PMID:
21167225
30.

Helicobacter pylori CagA inhibits endocytosis of cytotoxin VacA in host cells.

Akada JK, Aoki H, Torigoe Y, Kitagawa T, Kurazono H, Hoshida H, Nishikawa J, Terai S, Matsuzaki M, Hirayama T, Nakazawa T, Akada R, Nakamura K.

Dis Model Mech. 2010 Sep-Oct;3(9-10):605-17. doi: 10.1242/dmm.004879. Epub 2010 Aug 3.

31.

Random and targeted gene integrations through the control of non-homologous end joining in the yeast Kluyveromyces marxianus.

Abdel-Banat BM, Nonklang S, Hoshida H, Akada R.

Yeast. 2010 Jan;27(1):29-39. doi: 10.1002/yea.1729.

32.

High-temperature fermentation: how can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast?

Abdel-Banat BM, Hoshida H, Ano A, Nonklang S, Akada R.

Appl Microbiol Biotechnol. 2010 Jan;85(4):861-7. doi: 10.1007/s00253-009-2248-5. Epub 2009 Oct 10. Review.

PMID:
19820925
33.

Development of a novel functional high-throughput screening system for pathogen effectors in the yeast Saccharomyces cerevisiae.

Tabuchi M, Kawai Y, Nishie-Fujita M, Akada R, Izumi T, Yanatori I, Miyashita N, Ouchi K, Kishi F.

Biosci Biotechnol Biochem. 2009 Oct;73(10):2261-7. Epub 2009 Oct 7.

34.

Marker-disruptive gene integration and URA3 recycling for multiple gene manipulation in Saccharomyces cerevisiae.

Kaneko S, Tanaka T, Noda H, Fukuda H, Akada R, Kondo A.

Appl Microbiol Biotechnol. 2009 Jun;83(4):783-9. doi: 10.1007/s00253-009-2038-0. Epub 2009 May 20.

PMID:
19455322
35.

Construction of flocculent Kluyveromyces marxianus strains suitable for high-temperature ethanol fermentation.

Nonklang S, Ano A, Abdel-Banat BM, Saito Y, Hoshida H, Akada R.

Biosci Biotechnol Biochem. 2009 May;73(5):1090-5. Epub 2009 May 7.

36.

Combinatorial gene overexpression and recessive mutant gene introduction in sake yeast.

Ano A, Suehiro D, Cha-Aim K, Aritomi K, Phonimdaeng P, Nontaso N, Hoshida H, Mizunuma M, Miyakawa T, Akada R.

Biosci Biotechnol Biochem. 2009 Mar 23;73(3):633-40. Epub 2009 Mar 7.

37.

Reliable fusion PCR mediated by GC-rich overlap sequences.

Cha-aim K, Fukunaga T, Hoshida H, Akada R.

Gene. 2009 Apr 1;434(1-2):43-9. doi: 10.1016/j.gene.2008.12.014. Epub 2008 Dec 29.

PMID:
19159667
38.

High-temperature ethanol fermentation and transformation with linear DNA in the thermotolerant yeast Kluyveromyces marxianus DMKU3-1042.

Nonklang S, Abdel-Banat BM, Cha-aim K, Moonjai N, Hoshida H, Limtong S, Yamada M, Akada R.

Appl Environ Microbiol. 2008 Dec;74(24):7514-21. doi: 10.1128/AEM.01854-08. Epub 2008 Oct 17.

39.

Isolation of a novel mutant strain of Saccharomyces cerevisiae by an ethyl methane sulfonate-induced mutagenesis approach as a high producer of bioethanol.

Mobini-Dehkordi M, Nahvi I, Zarkesh-Esfahani H, Ghaedi K, Tavassoli M, Akada R.

J Biosci Bioeng. 2008 Apr;105(4):403-8. doi: 10.1263/jbb.105.403.

PMID:
18499058
40.

Loss of heterozygosity is induced in Candida albicans by ultraviolet irradiation.

Takagi Y, Akada R, Kumagai H, Yamamoto K, Tamaki H.

Appl Microbiol Biotechnol. 2008 Jan;77(5):1073-82. Epub 2007 Nov 17.

PMID:
18026727
41.

Screening of drugs that suppress Ste11 MAPKKK activation in yeast identified a c-Abl tyrosine kinase inhibitor.

Kitagawa T, Hashizume Y, Murakane T, Koga E, Nomura Y, Kakihara Y, Fujieda A, Uchida M, Takahashi H, Hoshida H, Akada R.

Biosci Biotechnol Biochem. 2007 Mar;71(3):772-82. Epub 2007 Mar 7.

42.

Genome-wide analysis of cellular response to bacterial genotoxin CdtB in yeast.

Kitagawa T, Hoshida H, Akada R.

Infect Immun. 2007 Mar;75(3):1393-402. Epub 2007 Jan 12.

43.

PCR-mediated seamless gene deletion and marker recycling in Saccharomyces cerevisiae.

Akada R, Kitagawa T, Kaneko S, Toyonaga D, Ito S, Kakihara Y, Hoshida H, Morimura S, Kondo A, Kida K.

Yeast. 2006 Apr 15;23(5):399-405.

44.

Genetically modified industrial yeast ready for application.

Akada R.

J Biosci Bioeng. 2002;94(6):536-44.

PMID:
16233347
45.

Isolation of five laccase gene sequences from the white-rot fungus Trametes sanguinea by PCR, and cloning, characterization and expression of the laccase cDNA in yeasts.

Hoshida H, Nakao M, Kanazawa H, Kubo K, Hakukawa T, Morimasa K, Akada R, Nishizawa Y.

J Biosci Bioeng. 2001;92(4):372-80.

PMID:
16233113
46.

Detection of a point mutation in FAS2 gene of sake yeast strains by allele-specific PCR amplification.

Akada R, Hirosawa I, Hoshida H, Nishizawa Y.

J Biosci Bioeng. 2001;92(2):189-92.

PMID:
16233083
47.
48.

Direct mating between diploid sake strains of Saccharomyces cerevisiae.

Hashimoto S, Aritomi K, Minohara T, Nishizawa Y, Hoshida H, Kashiwagi S, Akada R.

Appl Microbiol Biotechnol. 2006 Feb;69(6):689-96. Epub 2005 Jun 30.

PMID:
15988574
49.

Copper-dependent production of a Pycnoporus coccineus extracellular laccase in Aspergillus oryzae and Saccharomyces cerevisiae.

Hoshida H, Fujita T, Murata K, Kubo K, Akada R.

Biosci Biotechnol Biochem. 2005 Jun;69(6):1090-7.

50.

Isolation of auxotrophic mutants of diploid industrial yeast strains after UV mutagenesis.

Hashimoto S, Ogura M, Aritomi K, Hoshida H, Nishizawa Y, Akada R.

Appl Environ Microbiol. 2005 Jan;71(1):312-9.

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