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Insights into centriole geometry revealed by cryoTomography of doublet and triplet centrioles.

Greenan GA, Keszthelyi B, Vale RD, Agard DA.

Elife. 2018 Aug 6;7. pii: e36851. doi: 10.7554/eLife.36851. [Epub ahead of print]


The Psychiatric Cell Map Initiative: A Convergent Systems Biological Approach to Illuminating Key Molecular Pathways in Neuropsychiatric Disorders.

Willsey AJ, Morris MT, Wang S, Willsey HR, Sun N, Teerikorpi N, Baum TB, Cagney G, Bender KJ, Desai TA, Srivastava D, Davis GW, Doudna J, Chang E, Sohal V, Lowenstein DH, Li H, Agard D, Keiser MJ, Shoichet B, von Zastrow M, Mucke L, Finkbeiner S, Gan L, Sestan N, Ward ME, Huttenhain R, Nowakowski TJ, Bellen HJ, Frank LM, Khokha MK, Lifton RP, Kampmann M, Ideker T, State MW, Krogan NJ.

Cell. 2018 Jul 26;174(3):505-520. doi: 10.1016/j.cell.2018.06.016. Review.


A simple and robust procedure for preparing graphene-oxide cryo-EM grids.

Palovcak E, Wang F, Zheng SQ, Yu Z, Li S, Betegon M, Bulkley D, Agard DA, Cheng Y.

J Struct Biol. 2018 Jul 11. pii: S1047-8477(18)30165-5. doi: 10.1016/j.jsb.2018.07.007. [Epub ahead of print]


Calcium binding to a remote site can replace magnesium as cofactor for mitochondrial Hsp90 (TRAP1) ATPase activity.

Elnatan D, Agard DA.

J Biol Chem. 2018 Jul 10. pii: jbc.RA118.003562. doi: 10.1074/jbc.RA118.003562. [Epub ahead of print]


Structural basis of mitochondrial receptor binding and constriction by DRP1.

Kalia R, Wang RY, Yusuf A, Thomas PV, Agard DA, Shaw JM, Frost A.

Nature. 2018 Jun;558(7710):401-405. doi: 10.1038/s41586-018-0211-2. Epub 2018 Jun 13.


Cryo-tomography tilt-series alignment with consideration of the beam-induced sample motion.

Fernandez JJ, Li S, Bharat TAM, Agard DA.

J Struct Biol. 2018 Jun;202(3):200-209. doi: 10.1016/j.jsb.2018.02.001. Epub 2018 Feb 3.


The Structural Asymmetry of Mitochondrial Hsp90 (Trap1) Determines Fine Tuning of Functional Dynamics.

Moroni E, Agard DA, Colombo G.

J Chem Theory Comput. 2018 Feb 13;14(2):1033-1044. doi: 10.1021/acs.jctc.7b00766. Epub 2018 Jan 26.


The centrosomin CM2 domain is a multi-functional binding domain with distinct cell cycle roles.

Citron YR, Fagerstrom CJ, Keszthelyi B, Huang B, Rusan NM, Kelly MJS, Agard DA.

PLoS One. 2018 Jan 9;13(1):e0190530. doi: 10.1371/journal.pone.0190530. eCollection 2018.


Protein Expression and Purification of the Hsp90-Cdc37-Cdk4 Kinase Complex from Saccharomyces cerevisiae.

Verba KA, Agard DA.

Bio Protoc. 2017 Oct 5;7(19). pii: e2563. doi: 10.21769/BioProtoc.2563.


How Hsp90 and Cdc37 Lubricate Kinase Molecular Switches.

Verba KA, Agard DA.

Trends Biochem Sci. 2017 Oct;42(10):799-811. doi: 10.1016/j.tibs.2017.07.002. Epub 2017 Aug 4. Review.


Symmetry broken and rebroken during the ATP hydrolysis cycle of the mitochondrial Hsp90 TRAP1.

Elnatan D, Betegon M, Liu Y, Ramelot T, Kennedy MA, Agard DA.

Elife. 2017 Jul 25;6. pii: e25235. doi: 10.7554/eLife.25235.


NMR characterization of HtpG, the E. coli Hsp90, using sparse labeling with 13C-methyl alanine.

Pederson K, Chalmers GR, Gao Q, Elnatan D, Ramelot TA, Ma LC, Montelione GT, Kennedy MA, Agard DA, Prestegard JH.

J Biomol NMR. 2017 Jul;68(3):225-236. doi: 10.1007/s10858-017-0123-8. Epub 2017 Jun 26.


Liquid droplet formation by HP1α suggests a role for phase separation in heterochromatin.

Larson AG, Elnatan D, Keenen MM, Trnka MJ, Johnston JB, Burlingame AL, Agard DA, Redding S, Narlikar GJ.

Nature. 2017 Jul 13;547(7662):236-240. doi: 10.1038/nature22822. Epub 2017 Jun 21.


Grafting both acute wound site and adjacent donor site with the same graft: an easy and safe procedure to improve healing and minimize pain in elderly and bedridden patients.

Keilani C, Agard D, Duhoux A, Lakhel A, Giraud O, Brachet M, Duhamel P, Bey E.

Ann Burns Fire Disasters. 2017 Mar 31;30(1):52-56.


MotionCor2: anisotropic correction of beam-induced motion for improved cryo-electron microscopy.

Zheng SQ, Palovcak E, Armache JP, Verba KA, Cheng Y, Agard DA.

Nat Methods. 2017 Apr;14(4):331-332. doi: 10.1038/nmeth.4193. Epub 2017 Feb 27. No abstract available.


Design of Allosteric Stimulators of the Hsp90 ATPase as New Anticancer Leads.

D'Annessa I, Sattin S, Tao J, Pennati M, Sànchez-Martìn C, Moroni E, Rasola A, Zaffaroni N, Agard DA, Bernardi A, Colombo G.

Chemistry. 2017 Apr 19;23(22):5188-5192. doi: 10.1002/chem.201700169. Epub 2017 Mar 22.


Assembly of a nucleus-like structure during viral replication in bacteria.

Chaikeeratisak V, Nguyen K, Khanna K, Brilot AF, Erb ML, Coker JK, Vavilina A, Newton GL, Buschauer R, Pogliano K, Villa E, Agard DA, Pogliano J.

Science. 2017 Jan 13;355(6321):194-197. doi: 10.1126/science.aal2130.


Internal Structure and Preferential Protein Binding of Colloidal Aggregates.

Duan D, Torosyan H, Elnatan D, McLaughlin CK, Logie J, Shoichet MS, Agard DA, Shoichet BK.

ACS Chem Biol. 2017 Jan 20;12(1):282-290. doi: 10.1021/acschembio.6b00791. Epub 2016 Dec 16.


Atomic structure of Hsp90-Cdc37-Cdk4 reveals that Hsp90 traps and stabilizes an unfolded kinase.

Verba KA, Wang RY, Arakawa A, Liu Y, Shirouzu M, Yokoyama S, Agard DA.

Science. 2016 Jun 24;352(6293):1542-7. doi: 10.1126/science.aaf5023.


Higher-order oligomerization of Spc110p drives γ-tubulin ring complex assembly.

Lyon AS, Morin G, Moritz M, Yabut KC, Vojnar T, Zelter A, Muller E, Davis TN, Agard DA.

Mol Biol Cell. 2016 Jul 15;27(14):2245-58. doi: 10.1091/mbc.E16-02-0072. Epub 2016 May 25.


A Fortunate Story of an Unusual AK-47 Bullet Trajectory: Always Keep a Smartphone in Your Pocket.

Thabouillot O, Perrier P, Roche NC, Agard D, Barbier O, Martin G, Viant E, Leclere JB.

Prehosp Disaster Med. 2016 Jun;31(3):343-5. doi: 10.1017/S1049023X16000364. Epub 2016 Apr 18.


The Seckel syndrome and centrosomal protein Ninein localizes asymmetrically to stem cell centrosomes but is not required for normal development, behavior, or DNA damage response in Drosophila.

Zheng Y, Mennella V, Marks S, Wildonger J, Elnagdi E, Agard D, Megraw TL.

Mol Biol Cell. 2016 Jun 1;27(11):1740-52. doi: 10.1091/mbc.E15-09-0655. Epub 2016 Apr 6. Erratum in: Mol Biol Cell. 2016 Oct 15;27(20):3180.


Molecular Dynamics Simulations Reveal the Mechanisms of Allosteric Activation of Hsp90 by Designed Ligands.

Vettoretti G, Moroni E, Sattin S, Tao J, Agard DA, Bernardi A, Colombo G.

Sci Rep. 2016 Apr 1;6:23830. doi: 10.1038/srep23830.


Structure of γ-tubulin small complex based on a cryo-EM map, chemical cross-links, and a remotely related structure.

Greenberg CH, Kollman J, Zelter A, Johnson R, MacCoss MJ, Davis TN, Agard DA, Sali A.

J Struct Biol. 2016 Jun;194(3):303-10. doi: 10.1016/j.jsb.2016.03.006. Epub 2016 Mar 8.


Clinical validation of Anyplex™ II HPV HR Detection according to the guidelines for HPV test requirements for cervical cancer screening.

Hesselink AT, Sahli R, Berkhof J, Snijders PJ, van der Salm ML, Agard D, Bleeker MC, Heideman DA.

J Clin Virol. 2016 Mar;76:36-9. doi: 10.1016/j.jcv.2016.01.009. Epub 2016 Jan 15.


Asynchronous data acquisition and on-the-fly analysis of dose fractionated cryoEM images by UCSFImage.

Li X, Zheng S, Agard DA, Cheng Y.

J Struct Biol. 2015 Nov;192(2):174-8. doi: 10.1016/j.jsb.2015.09.003. Epub 2015 Sep 11.


Activity-Independent Discovery of Secondary Metabolites Using Chemical Elicitation and Cheminformatic Inference.

Pimentel-Elardo SM, Sørensen D, Ho L, Ziko M, Bueler SA, Lu S, Tao J, Moser A, Lee R, Agard D, Fairn G, Rubinstein JL, Shoichet BK, Nodwell JR.

ACS Chem Biol. 2015 Nov 20;10(11):2616-23. doi: 10.1021/acschembio.5b00612. Epub 2015 Sep 18.


Evaluation of super-resolution performance of the K2 electron-counting camera using 2D crystals of aquaporin-0.

Chiu PL, Li X, Li Z, Beckett B, Brilot AF, Grigorieff N, Agard DA, Cheng Y, Walz T.

J Struct Biol. 2015 Nov;192(2):163-73. doi: 10.1016/j.jsb.2015.08.015. Epub 2015 Aug 28.


Activation of Hsp90 Enzymatic Activity and Conformational Dynamics through Rationally Designed Allosteric Ligands.

Sattin S, Tao J, Vettoretti G, Moroni E, Pennati M, Lopergolo A, Morelli L, Bugatti A, Zuehlke A, Moses M, Prince T, Kijima T, Beebe K, Rusnati M, Neckers L, Zaffaroni N, Agard DA, Bernardi A, Colombo G.

Chemistry. 2015 Sep 21;21(39):13598-608. doi: 10.1002/chem.201502211. Epub 2015 Aug 18.


The cancer cell map initiative: defining the hallmark networks of cancer.

Krogan NJ, Lippman S, Agard DA, Ashworth A, Ideker T.

Mol Cell. 2015 May 21;58(4):690-8. doi: 10.1016/j.molcel.2015.05.008. Review.


Interaction of CK1δ with γTuSC ensures proper microtubule assembly and spindle positioning.

Peng Y, Moritz M, Han X, Giddings TH, Lyon A, Kollman J, Winey M, Yates J 3rd, Agard DA, Drubin DG, Barnes G.

Mol Biol Cell. 2015 Jul 1;26(13):2505-18. doi: 10.1091/mbc.E14-12-1627. Epub 2015 May 13.


Hsp70 forms antiparallel dimers stabilized by post-translational modifications to position clients for transfer to Hsp90.

Morgner N, Schmidt C, Beilsten-Edmands V, Ebong IO, Patel NA, Clerico EM, Kirschke E, Daturpalli S, Jackson SE, Agard D, Robinson CV.

Cell Rep. 2015 May 5;11(5):759-69. doi: 10.1016/j.celrep.2015.03.063. Epub 2015 Apr 23.


MRC2014: Extensions to the MRC format header for electron cryo-microscopy and tomography.

Cheng A, Henderson R, Mastronarde D, Ludtke SJ, Schoenmakers RH, Short J, Marabini R, Dallakyan S, Agard D, Winn M.

J Struct Biol. 2015 Nov;192(2):146-50. doi: 10.1016/j.jsb.2015.04.002. Epub 2015 Apr 13.


Ring closure activates yeast γTuRC for species-specific microtubule nucleation.

Kollman JM, Greenberg CH, Li S, Moritz M, Zelter A, Fong KK, Fernandez JJ, Sali A, Kilmartin J, Davis TN, Agard DA.

Nat Struct Mol Biol. 2015 Feb;22(2):132-7. doi: 10.1038/nsmb.2953. Epub 2015 Jan 19.


A novel N-terminal extension in mitochondrial TRAP1 serves as a thermal regulator of chaperone activity.

Partridge JR, Lavery LA, Elnatan D, Naber N, Cooke R, Agard DA.

Elife. 2014 Dec 22;3. doi: 10.7554/eLife.03487.


A bacteriophage tubulin harnesses dynamic instability to center DNA in infected cells.

Erb ML, Kraemer JA, Coker JK, Chaikeeratisak V, Nonejuie P, Agard DA, Pogliano J.

Elife. 2014 Nov 27;3. doi: 10.7554/eLife.03197.


Glucocorticoid receptor function regulated by coordinated action of the Hsp90 and Hsp70 chaperone cycles.

Kirschke E, Goswami D, Southworth D, Griffin PR, Agard DA.

Cell. 2014 Jun 19;157(7):1685-97. doi: 10.1016/j.cell.2014.04.038.


Elucidating the mechanism of substrate recognition by the bacterial Hsp90 molecular chaperone.

Street TO, Zeng X, Pellarin R, Bonomi M, Sali A, Kelly MJ, Chu F, Agard DA.

J Mol Biol. 2014 Jun 12;426(12):2393-404. doi: 10.1016/j.jmb.2014.04.001. Epub 2014 Apr 12.


Microarray-based screening of heat shock protein inhibitors.

Schax E, Walter JG, Märzhäuser H, Stahl F, Scheper T, Agard DA, Eichner S, Kirschning A, Zeilinger C.

J Biotechnol. 2014 Jun 20;180:1-9. doi: 10.1016/j.jbiotec.2014.03.006. Epub 2014 Mar 22.


Sparse labeling of proteins: structural characterization from long range constraints.

Prestegard JH, Agard DA, Moremen KW, Lavery LA, Morris LC, Pederson K.

J Magn Reson. 2014 Apr;241:32-40. doi: 10.1016/j.jmr.2013.12.012. Review.


The structure and assembly mechanism of a novel three-stranded tubulin filament that centers phage DNA.

Zehr EA, Kraemer JA, Erb ML, Coker JK, Montabana EA, Pogliano J, Agard DA.

Structure. 2014 Apr 8;22(4):539-48. doi: 10.1016/j.str.2014.02.006. Epub 2014 Mar 13.


Bacterial tubulin TubZ-Bt transitions between a two-stranded intermediate and a four-stranded filament upon GTP hydrolysis.

Montabana EA, Agard DA.

Proc Natl Acad Sci U S A. 2014 Mar 4;111(9):3407-12. doi: 10.1073/pnas.1318339111. Epub 2014 Feb 18.


The free energy profile of tubulin straight-bent conformational changes, with implications for microtubule assembly and drug discovery.

Peng LX, Hsu MT, Bonomi M, Agard DA, Jacobson MP.

PLoS Comput Biol. 2014 Feb 6;10(2):e1003464. doi: 10.1371/journal.pcbi.1003464. eCollection 2014 Feb.


Structural asymmetry in the closed state of mitochondrial Hsp90 (TRAP1) supports a two-step ATP hydrolysis mechanism.

Lavery LA, Partridge JR, Ramelot TA, Elnatan D, Kennedy MA, Agard DA.

Mol Cell. 2014 Jan 23;53(2):330-43. doi: 10.1016/j.molcel.2013.12.023.


Amorphous no more: subdiffraction view of the pericentriolar material architecture.

Mennella V, Agard DA, Huang B, Pelletier L.

Trends Cell Biol. 2014 Mar;24(3):188-97. doi: 10.1016/j.tcb.2013.10.001. Epub 2013 Nov 19. Review.


Cell-cycle regulation of formin-mediated actin cable assembly.

Miao Y, Wong CC, Mennella V, Michelot A, Agard DA, Holt LJ, Yates JR 3rd, Drubin DG.

Proc Natl Acad Sci U S A. 2013 Nov 19;110(47):E4446-55. doi: 10.1073/pnas.1314000110. Epub 2013 Oct 16.


A new approach for the laboratory culture of the fathead minnow, Pimephales promelas.

Gordon DA, Smith ME, Wratschko M, Agard D, Holden L, Wilcox S, Lazorchak JM.

Environ Toxicol Chem. 2014 Jan;33(1):126-33. doi: 10.1002/etc.2396. Epub 2013 Nov 5.


High-resolution restoration of 3D structures from widefield images with extreme low signal-to-noise-ratio.

Arigovindan M, Fung JC, Elnatan D, Mennella V, Chan YH, Pollard M, Branlund E, Sedat JW, Agard DA.

Proc Natl Acad Sci U S A. 2013 Oct 22;110(43):17344-9. doi: 10.1073/pnas.1315675110. Epub 2013 Oct 8.


The Aptima HPV assay fulfills the cross-sectional clinical and reproducibility criteria of international guidelines for human papillomavirus test requirements for cervical screening.

Heideman DA, Hesselink AT, van Kemenade FJ, Iftner T, Berkhof J, Topal F, Agard D, Meijer CJ, Snijders PJ.

J Clin Microbiol. 2013 Nov;51(11):3653-7. doi: 10.1128/JCM.01517-13. Epub 2013 Aug 28.


Influence of electron dose rate on electron counting images recorded with the K2 camera.

Li X, Zheng SQ, Egami K, Agard DA, Cheng Y.

J Struct Biol. 2013 Nov;184(2):251-60. doi: 10.1016/j.jsb.2013.08.005. Epub 2013 Aug 20.

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