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Nat Methods. 2018 Mar;15(3):201-206. doi: 10.1038/nmeth.4577. Epub 2018 Jan 15.

Highly parallel direct RNA sequencing on an array of nanopores.

Author information

1
Oxford Nanopore Technologies Ltd., Oxford, UK.
2
Oxford Nanopore Technologies Inc., New York, New York, USA.

Abstract

Sequencing the RNA in a biological sample can unlock a wealth of information, including the identity of bacteria and viruses, the nuances of alternative splicing or the transcriptional state of organisms. However, current methods have limitations due to short read lengths and reverse transcription or amplification biases. Here we demonstrate nanopore direct RNA-seq, a highly parallel, real-time, single-molecule method that circumvents reverse transcription or amplification steps. This method yields full-length, strand-specific RNA sequences and enables the direct detection of nucleotide analogs in RNA.

PMID:
29334379
DOI:
10.1038/nmeth.4577

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