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J Craniomaxillofac Surg. 2018 Sep;46(9):1593-1600. doi: 10.1016/j.jcms.2018.05.049. Epub 2018 Jun 2.

Combining autologous bone marrow mononuclear cells seeded on collagen sponge with Nano Hydroxyapatite, and platelet-rich fibrin: Reporting a novel strategy for alveolar cleft bone regeneration.

Author information

1
Al-Azhar Cleft Lip and Palate Treatment Center, Faculty of Dental Medicine for Girls, Al-Azhar University, 1 Al Mokhaym Al Daem, Cairo, Egypt.
2
Al-Azhar Cleft Lip and Palate Treatment Center, Faculty of Dental Medicine for Girls, Al-Azhar University, 1 Al Mokhaym Al Daem, Cairo, Egypt; Department of Oro-dental Genetics, Center of Medical Excellence, National Research Centre, 33 El Buhouth St., Dokki, Cairo, Egypt; Stem Cell Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, 33 El Buhouth St., Dokki, Cairo, Egypt.
3
Department of Oro-dental Genetics, Center of Medical Excellence, National Research Centre, 33 El Buhouth St., Dokki, Cairo, Egypt; Stem Cell Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, 33 El Buhouth St., Dokki, Cairo, Egypt. Electronic address: nermeenkandel@gmail.com.
4
Nasr City Hospital, 23 Youssef Abbas St. Off El Tayaran St., Nasr City, Cairo, Egypt.
5
Ganzouri Specialized Hospital, 63 Tomanbay St., Tahra Sq., Roxy, Heliopolis, Cairo, Egypt.
6
Department of Oro-dental Genetics, Center of Medical Excellence, National Research Centre, 33 El Buhouth St., Dokki, Cairo, Egypt.

Abstract

INTRODUCTION:

The aim of this study was to examine and assess the use of autologous bone marrow mononuclear cells (BMMNCs) combined with platelet-rich fibrin (PRF) and nanohydroxyapatite for bone regeneration as an effective technique for alveolar cleft repair.

PATIENTS AND METHODS:

This study included 20 patients with unilateral alveolar cleft defects and with an age range of 8-15 years. They were divided equally into two groups: Group A, received treatment via the regenerative approach which includes; autologous BMMNCs seeded on a collagen sponge in combination with nanohydroxyapatite and autologous PRF. Group B, received the standard alveolar bone grafting with iliac crest bone. The effectiveness of the new technique was evaluated and compared to the standard grafting technique through a 12-month follow-up via clinical and radiographic assessments.

RESULTS:

During the 12-month follow-up, Group A exhibited less donor site complications, faster and better soft tissue healing, and less postoperative pain, when compared to group B. 90% of the cases in group A, exhibited complete alveolar bone union verses 70% only in group B.

CONCLUSION:

Combination of BMMNCs, nanohydroxyapatite, and PRF greatly promote bone regeneration in alveolar cleft defects providing an alternative novel therapeutic strategy to the standard alveolar bone grafting.

KEYWORDS:

Alveolar cleft; Bone marrow stem cells; Bone regeneration; Platelet-rich fibrin

PMID:
30196860
DOI:
10.1016/j.jcms.2018.05.049
[Indexed for MEDLINE]

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