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Items: 1 to 50 of 95

1.

Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery.

Xu P, Modavi C, Demaree B, Twigg F, Liang B, Sun C, Zhang W, Abate AR.

Nucleic Acids Res. 2020 Feb 25. pii: gkaa131. doi: 10.1093/nar/gkaa131. [Epub ahead of print]

PMID:
32095820
2.

Linked optical and gene expression profiling of single cells at high-throughput.

Zhang JQ, Siltanen CA, Liu L, Chang KC, Gartner ZJ, Abate AR.

Genome Biol. 2020 Feb 24;21(1):49. doi: 10.1186/s13059-020-01958-9.

3.

Multiplatform Molecular Profiling Reveals Epigenomic Intratumor Heterogeneity in Ependymoma.

Liu SJ, Magill ST, Vasudevan HN, Hilz S, Villanueva-Meyer JE, Lastella S, Daggubati V, Spatz J, Choudhury A, Orr BA, Demaree B, Seo K, Ferris SP, Abate AR, Oberheim Bush NA, Bollen AW, McDermott MW, Costello JF, Raleigh DR.

Cell Rep. 2020 Feb 4;30(5):1300-1309.e5. doi: 10.1016/j.celrep.2020.01.018.

4.

Discovery of Stable and Selective Antibody Mimetics from Combinatorial Libraries of Polyvalent, Loop-Functionalized Peptoid Nanosheets.

Kim JH, Kim SC, Kline MA, Grzincic EM, Tresca BW, Cardiel J, Karbaschi M, Dehigaspitiya DC, Chen Y, Udumula V, Jian T, Murray DJ, Yun L, Connolly MD, Liu J, Ren G, Chen CL, Kirshenbaum K, Abate AR, Zuckermann RN.

ACS Nano. 2020 Jan 28;14(1):185-195. doi: 10.1021/acsnano.9b07498. Epub 2019 Dec 2.

PMID:
31789500
5.

Uniform, Large-Area, Highly Ordered Peptoid Monolayer and Bilayer Films for Sensing Applications.

Murray DJ, Kim JH, Grzincic EM, Kim SC, Abate AR, Zuckermann RN.

Langmuir. 2019 Oct 22;35(42):13671-13680. doi: 10.1021/acs.langmuir.9b02557. Epub 2019 Oct 11.

PMID:
31603340
6.

Human intestinal spheroids cultured using Sacrificial Micromolding as a model system for studying drug transport.

Samy KE, Levy ES, Phong K, Demaree B, Abate AR, Desai TA.

Sci Rep. 2019 Jul 9;9(1):9936. doi: 10.1038/s41598-019-46408-0.

7.

High throughput gene expression profiling of yeast colonies with microgel-culture Drop-seq.

Liu L, Dalal CK, Heineike BM, Abate AR.

Lab Chip. 2019 May 14;19(10):1838-1849. doi: 10.1039/c9lc00084d.

PMID:
31020292
8.

Reference-based analysis of lung single-cell sequencing reveals a transitional profibrotic macrophage.

Aran D, Looney AP, Liu L, Wu E, Fong V, Hsu A, Chak S, Naikawadi RP, Wolters PJ, Abate AR, Butte AJ, Bhattacharya M.

Nat Immunol. 2019 Feb;20(2):163-172. doi: 10.1038/s41590-018-0276-y. Epub 2019 Jan 14.

9.

Direct quantification of EGFR variant allele frequency in cell-free DNA using a microfluidic-free digital droplet PCR assay.

Demaree B, Weisgerber D, Dolatmoradi A, Hatori M, Abate AR.

Methods Cell Biol. 2018;148:119-131. doi: 10.1016/bs.mcb.2018.10.002. Epub 2018 Nov 1.

PMID:
30473066
10.

Microfluidic bead encapsulation above 20 kHz with triggered drop formation.

Clark IC, Abate AR.

Lab Chip. 2018 Dec 7;18(23):3598-3605. doi: 10.1039/c8lc00514a. Epub 2018 Oct 26.

11.

Particle-Templated Emulsification for Microfluidics-Free Digital Biology.

Hatori MN, Kim SC, Abate AR.

Anal Chem. 2018 Aug 21;90(16):9813-9820. doi: 10.1021/acs.analchem.8b01759. Epub 2018 Aug 1.

12.

An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing.

Demaree B, Weisgerber D, Lan F, Abate AR.

J Vis Exp. 2018 May 23;(135). doi: 10.3791/57598.

13.

An Oil-Free Picodrop Bioassay Platform for Synthetic Biology.

Siltanen CA, Cole RH, Poust S, Chao L, Tyerman J, Kaufmann-Malaga B, Ubersax J, Gartner ZJ, Abate AR.

Sci Rep. 2018 May 21;8(1):7913. doi: 10.1038/s41598-018-25577-4.

14.

A comparative analysis of single cell and droplet-based FACS for improving production phenotypes: Riboflavin overproduction in Yarrowia lipolytica.

Wagner JM, Liu L, Yuan SF, Venkataraman MV, Abate AR, Alper HS.

Metab Eng. 2018 May;47:346-356. doi: 10.1016/j.ymben.2018.04.015. Epub 2018 Apr 23.

PMID:
29698778
15.

Combined aptamer and transcriptome sequencing of single cells.

Delley CL, Liu L, Sarhan MF, Abate AR.

Sci Rep. 2018 Feb 13;8(1):2919. doi: 10.1038/s41598-018-21153-y.

16.

Concentric electrodes improve microfluidic droplet sorting.

Clark IC, Thakur R, Abate AR.

Lab Chip. 2018 Feb 27;18(5):710-713. doi: 10.1039/c7lc01242j.

17.

Single-Cell RT-PCR in Microfluidic Droplets with Integrated Chemical Lysis.

Kim SC, Clark IC, Shahi P, Abate AR.

Anal Chem. 2018 Jan 16;90(2):1273-1279. doi: 10.1021/acs.analchem.7b04050. Epub 2018 Jan 3.

18.

Bulk double emulsification for flow cytometric analysis of microfluidic droplets.

Sukovich DJ, Kim SC, Ahmed N, Abate AR.

Analyst. 2017 Dec 4;142(24):4618-4622. doi: 10.1039/c7an01695f.

19.

RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes.

Abatemarco J, Sarhan MF, Wagner JM, Lin JL, Liu L, Hassouneh W, Yuan SF, Alper HS, Abate AR.

Nat Commun. 2017 Aug 23;8(1):332. doi: 10.1038/s41467-017-00425-7.

20.

Rapid, chemical-free breaking of microfluidic emulsions with a hand-held antistatic gun.

Karbaschi M, Shahi P, Abate AR.

Biomicrofluidics. 2017 Jul 20;11(4):044107. doi: 10.1063/1.4995479. eCollection 2017 Jul.

21.

Cell-free extract based optimization of biomolecular circuits with droplet microfluidics.

Hori Y, Kantak C, Murray RM, Abate AR.

Lab Chip. 2017 Sep 12;17(18):3037-3042. doi: 10.1039/c7lc00552k.

PMID:
28770936
22.

Printed droplet microfluidics for on demand dispensing of picoliter droplets and cells.

Cole RH, Tang SY, Siltanen CA, Shahi P, Zhang JQ, Poust S, Gartner ZJ, Abate AR.

Proc Natl Acad Sci U S A. 2017 Aug 15;114(33):8728-8733. doi: 10.1073/pnas.1704020114. Epub 2017 Jul 31.

23.

Efficient extraction of oil from droplet microfluidic emulsions.

Haliburton JR, Kim SC, Clark IC, Sperling RA, Weitz DA, Abate AR.

Biomicrofluidics. 2017 May 19;11(3):034111. doi: 10.1063/1.4984035. eCollection 2017 May.

24.

Single-cell genome sequencing at ultra-high-throughput with microfluidic droplet barcoding.

Lan F, Demaree B, Ahmed N, Abate AR.

Nat Biotechnol. 2017 Jul;35(7):640-646. doi: 10.1038/nbt.3880. Epub 2017 May 29.

25.

Finding a helix in a haystack: nucleic acid cytometry with droplet microfluidics.

Clark IC, Abate AR.

Lab Chip. 2017 Jun 13;17(12):2032-2045. doi: 10.1039/c7lc00241f. Review.

26.

Abseq: Ultrahigh-throughput single cell protein profiling with droplet microfluidic barcoding.

Shahi P, Kim SC, Haliburton JR, Gartner ZJ, Abate AR.

Sci Rep. 2017 Mar 14;7:44447. doi: 10.1038/srep44447.

27.

Genetic interaction mapping with microfluidic-based single cell sequencing.

Haliburton JR, Shao W, Deutschbauer A, Arkin A, Abate AR.

PLoS One. 2017 Feb 7;12(2):e0171302. doi: 10.1371/journal.pone.0171302. eCollection 2017.

28.

Sequence specific sorting of DNA molecules with FACS using 3dPCR.

Sukovich DJ, Lance ST, Abate AR.

Sci Rep. 2017 Jan 4;7:39385. doi: 10.1038/srep39385.

29.

PCR-activated cell sorting as a general, cultivation-free method for high-throughput identification and enrichment of virus hosts.

Lim SW, Lance ST, Stedman KM, Abate AR.

J Virol Methods. 2017 Apr;242:14-21. doi: 10.1016/j.jviromet.2016.12.009. Epub 2016 Dec 29.

30.

Measurement of copy number variation in single cancer cells using rapid-emulsification digital droplet MDA.

Kim SC, Premasekharan G, Clark IC, Gemeda HB, Paris PL, Abate AR.

Microsyst Nanoeng. 2017;3. pii: 17018. doi: 10.1038/micronano.2017.18. Epub 2017 Jun 19.

31.

Peering below the diffraction limit: robust and specific sorting of viruses with flow cytometry.

Lance ST, Sukovich DJ, Stedman KM, Abate AR.

Virol J. 2016 Dec 1;13(1):201.

32.

Operation of Droplet-Microfluidic Devices with a Lab Centrifuge.

Ahmed N, Sukovich D, Abate AR.

Micromachines (Basel). 2016 Sep 6;7(9). pii: E161. doi: 10.3390/mi7090161.

33.

Droplet barcoding for massively parallel single-molecule deep sequencing.

Lan F, Haliburton JR, Yuan A, Abate AR.

Nat Commun. 2016 Jun 29;7:11784. doi: 10.1038/ncomms11784.

34.

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers.

Cole RH, Gartner ZJ, Abate AR.

J Vis Exp. 2016 May 5;(111). doi: 10.3791/54010.

35.

Electrical lysis of cells for detergent-free droplet assays.

de Lange N, Tran TM, Abate AR.

Biomicrofluidics. 2016 Mar 22;10(2):024114. doi: 10.1063/1.4944742. eCollection 2016 Mar.

36.

Double Emulsion Generation Using a Polydimethylsiloxane (PDMS) Co-axial Flow Focus Device.

Cole RH, Tran TM, Abate AR.

J Vis Exp. 2015 Dec 25;(106):e53516. doi: 10.3791/53516.

37.

Enhanced sequencing coverage with digital droplet multiple displacement amplification.

Sidore AM, Lan F, Lim SW, Abate AR.

Nucleic Acids Res. 2016 Apr 20;44(7):e66. doi: 10.1093/nar/gkv1493. Epub 2015 Dec 23.

38.

Patterning microfluidic device wettability with spatially-controlled plasma oxidation.

Kim SC, Sukovich DJ, Abate AR.

Lab Chip. 2015 Aug 7;15(15):3163-9. doi: 10.1039/c5lc00626k.

39.

Dissecting enzyme function with microfluidic-based deep mutational scanning.

Romero PA, Tran TM, Abate AR.

Proc Natl Acad Sci U S A. 2015 Jun 9;112(23):7159-64. doi: 10.1073/pnas.1422285112. Epub 2015 May 26.

40.

Compact and modular multicolour fluorescence detector for droplet microfluidics.

Cole RH, de Lange N, Gartner ZJ, Abate AR.

Lab Chip. 2015 Jul 7;15(13):2754-8. doi: 10.1039/c5lc00333d. Epub 2015 Jun 2.

41.

Microfluidic droplet enrichment for targeted sequencing.

Eastburn DJ, Huang Y, Pellegrino M, Sciambi A, Ptáček LJ, Abate AR.

Nucleic Acids Res. 2015 Jul 27;43(13):e86. doi: 10.1093/nar/gkv297. Epub 2015 Apr 14.

42.

PCR-activated cell sorting for cultivation-free enrichment and sequencing of rare microbes.

Lim SW, Tran TM, Abate AR.

PLoS One. 2015 Jan 28;10(1):e0113549. doi: 10.1371/journal.pone.0113549. eCollection 2015.

43.

Rapid modulation of droplet composition with pincer microvalves.

Ochs CJ, Abate AR.

Lab Chip. 2015 Jan 7;15(1):52-6. doi: 10.1039/c4lc01040j.

44.

Accurate microfluidic sorting of droplets at 30 kHz.

Sciambi A, Abate AR.

Lab Chip. 2015 Jan 7;15(1):47-51. doi: 10.1039/c4lc01194e.

45.

Identification and genetic analysis of cancer cells with PCR-activated cell sorting.

Eastburn DJ, Sciambi A, Abate AR.

Nucleic Acids Res. 2014;42(16):e128. doi: 10.1093/nar/gku606. Epub 2014 Jul 16.

46.

Coaxial flow focusing in poly(dimethylsiloxane) microfluidic devices.

Tran TM, Cater S, Abate AR.

Biomicrofluidics. 2014 Feb 3;8(1):016502. doi: 10.1063/1.4863576. eCollection 2014 Jan.

47.

Generating electric fields in PDMS microfluidic devices with salt water electrodes.

Sciambi A, Abate AR.

Lab Chip. 2014 Aug 7;14(15):2605-9. doi: 10.1039/c4lc00078a. Epub 2014 Mar 27.

48.

Adding reagent to droplets with controlled rupture of encapsulated double emulsions.

Sciambi A, Abate AR.

Biomicrofluidics. 2013 Aug 5;7(4):44112. doi: 10.1063/1.4817793. eCollection 2013.

49.

Block-and-break generation of microdroplets with fixed volume.

van Steijn V, Korczyk PM, Derzsi L, Abate AR, Weitz DA, Garstecki P.

Biomicrofluidics. 2013 Apr 10;7(2):24108. doi: 10.1063/1.4801637. eCollection 2013.

50.

DNA sequence analysis with droplet-based microfluidics.

Abate AR, Hung T, Sperling RA, Mary P, Rotem A, Agresti JJ, Weiner MA, Weitz DA.

Lab Chip. 2013 Dec 21;13(24):4864-9. doi: 10.1039/c3lc50905b.

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