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Environ Microbiol. 2016 Dec;18(12):4471-4484. doi: 10.1111/1462-2920.13433. Epub 2016 Jul 18.

Metagenomics unveils the attributes of the alginolytic guilds of sediments from four distant cold coastal environments.

Author information

1
Laboratorio de Microbiología Ambiental, Centro para el Estudio de Sistemas Marinos (CESIMAR, CONICET), Puerto Madryn, U9120ACD, Argentina.
2
Architecture et Fonction des Macromolécules Biologiques, CNRS, Aix-Marseille Université, Marseille, 13288, France.
3
INRA, USC 1408 AFMB, Marseille, F-13288, France.
4
Department of Biological Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia.
5
Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, 99352, USA.
6
Instituto Antártico Argentino, Ciudad Autónoma de Buenos Aires, C1064ABR, Argentina.
7
Instituto Nanobiotec, CONICET - Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, C1113AAC, Argentina.
8
Akvaplan-niva, Fram - High North Research Centre for Climate and the Environment, Tromsø, NO-9296, Norway.
9
CAGE - Centre for Arctic Gas Hydrate, Environment and Climate, UiT The Arctic University of Norway, Tromsø, N-9037, Norway.
10
School of Natural Sciences and Environmental Studies, Södertörn University, Huddinge, 141 89, Sweden.
11
Stockholm University, Stockholm, SE-106 91, Sweden.

Abstract

Alginates are abundant polysaccharides in brown algae that constitute an important energy source for marine heterotrophic bacteria. Despite the key role of alginate degradation processes in the marine carbon cycle, little information is available on the bacterial populations involved in these processes. The aim of this work was to gain a better understanding of alginate utilization capabilities in cold coastal environments. Sediment metagenomes from four high-latitude regions of both Hemispheres were interrogated for alginate lyase gene homologue sequences and their genomic context. Sediments contained highly abundant and diverse bacterial assemblages with alginolytic potential, including members of Bacteroidetes and Proteobacteria, as well as several poorly characterized taxa. The microbial communities in Arctic and Antarctic sediments exhibited the most similar alginolytic profiles, whereas brackish sediments showed distinct structures with a higher proportion of novel genes. Examination of the gene neighbourhood of the alginate lyase homologues revealed distinct patterns depending on the potential lineage of the scaffolds, with evidence of evolutionary relationships among alginolytic gene clusters from Bacteroidetes and Proteobacteria. This information is relevant for understanding carbon fluxes in cold coastal environments and provides valuable information for the development of biotechnological applications from brown algae biomass.

PMID:
27348213
DOI:
10.1111/1462-2920.13433
[Indexed for MEDLINE]

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