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J Biol Chem. 2013 Dec 27;288(52):36890-901. doi: 10.1074/jbc.M113.517797. Epub 2013 Nov 19.

Structure and function of the peanut panallergen Ara h 8.

Author information

1
From the Southern Regional Research Center, Agricultural Research Service, United States Department of Agriculture, New Orleans, Louisiana 70124.

Abstract

The incidence of peanut allergy continues to rise in the United States and Europe. Whereas exposure to the major allergens Ara h 1, 2, 3, and 6 can cause fatal anaphylaxis, exposure to the minor allergens usually does not. Ara h 8 is a minor allergen. Importantly, it is the minor food allergens that are thought to be responsible for oral allergy syndrome (OAS), in which sensitization to airborne allergens causes a Type 2 allergic reaction to ingested foods. Furthermore, it is believed that similar protein structure rather than a similar linear sequence is the cause of OAS. Bet v 1 from birch pollen is a common sensitizing agent, and OAS results when patients consume certain fruits, vegetables, tree nuts, and peanuts. Here, we report the three-dimensional structure of Ara h 8, a Bet v 1 homolog. The overall fold is very similar to that of Bet v 1, Api g 1 (celery), Gly m 4 (soy), and Pru av 1 (cherry). Ara h 8 binds the isoflavones quercetin and apigenin as well as resveratrol avidly.

KEYWORDS:

Allergen; Antigen; Crystal Structure; Flavonoids; Fluorescence; Peanut

PMID:
24253038
PMCID:
PMC3873548
DOI:
10.1074/jbc.M113.517797
[Indexed for MEDLINE]
Free PMC Article

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