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Acta Crystallogr D Biol Crystallogr. 2013 Mar;69(Pt 3):464-70. doi: 10.1107/S0907444912050135. Epub 2013 Feb 16.

Ultratight crystal packing of a 10 kDa protein.

Author information

1
Proteolysis Laboratory, Department of Structural Biology, Molecular Biology Institute of Barcelona, Spanish Research Council CSIC, Barcelona Science Park, c/Baldiri Reixac 15-21, 08028 Barcelona, Spain.

Abstract

While small organic molecules generally crystallize forming tightly packed lattices with little solvent content, proteins form air-sensitive high-solvent-content crystals. Here, the crystallization and full structure analysis of a novel recombinant 10 kDa protein corresponding to the C-terminal domain of a putative U32 peptidase are reported. The orthorhombic crystal contained only 24.5% solvent and is therefore among the most tightly packed protein lattices ever reported.

KEYWORDS:

crystal packing; high resolution; solvent content

PMID:
23519421
PMCID:
PMC4048058
DOI:
10.1107/S0907444912050135
[Indexed for MEDLINE]
Free PMC Article

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