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Mol Plant. 2009 Jul;2(4):688-699. doi: 10.1093/mp/ssp026. Epub 2009 May 19.

A truncated Arabidopsis NUCLEOSOME ASSEMBLY PROTEIN 1, AtNAP1;3T, alters plant growth responses to abscisic acid and salt in the Atnap1;3-2 mutant.

Author information

1
Institut de Biologie Moléculaire des Plantes (IBMP) du CNRS, Université de Strasbourg (UdS), 12 rue du Général Zimmer, 67084 Strasbourg Cédex, France; State Key Laboratory of Genetic Engineering, Institute of Plant Biology, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China.
2
State Key Laboratory of Genetic Engineering, Institute of Plant Biology, Department of Biochemistry, School of Life Sciences, Fudan University, Shanghai 200433, People's Republic of China. Electronic address: aiwudong@fudan.edu.cn.
3
Institut de Biologie Moléculaire des Plantes (IBMP) du CNRS, Université de Strasbourg (UdS), 12 rue du Général Zimmer, 67084 Strasbourg Cédex, France. Electronic address: Wen-Hui.Shen@ibmp-ulp.u-strasbg.fr.

Abstract

Chromatin remodeling is thought to have crucial roles in plant adaptive response to environmental stimulus. Here, we report that, in Arabidopsis, the evolutionarily conserved histone chaperone, NUCLEOSOME ASSEMBLY PROTEIN 1 (NAP1), is involved in plant response to abscisic acid (ABA), a phytohormone important in stress adaptation. We show that simultaneous loss-of-function of AtNAP1;1, AtNAP1;2, and AtNAP1;3 (the triple mutant m123-1) caused a slight hypersensitive response to ABA in seedling growth. Strikingly, the other triple mutant m123-2 containing a different mutant allele of AtNAP1;3, the Atnap1;3-2 allele, showed a hyposensitive response to ABA and a decreased tolerance to salt stress. This ABA-hyposensitive and salt response phenotype specifically associated with the Atnap1;3-2 mutant allele. We show that this mutant allele produced a truncated protein, AtNAP1;3T, which lacks 34 amino acids at the C-terminus compared to the wild-type protein AtNAP1;3. We further show that the heterozygous plants containing the Atnap1;3-2 mutant allele as well as transgenic plants overexpressing AtNAP1;3T exhibit ABA-hyposensitive phenotype. It thus indicates that AtNAP1;3T functions as a dominant negative factor in ABA response. The expression of some ABA-responsive genes, including genes encoding protein kinases and transcription regulators, was found perturbed in the mutant and in the AtNAP1;3T transgenic plants. Taken together, our study uncovered AtNAP1 proteins as positive regulators and AtNAP1;3T as a negative regulator in ABA signaling pathways, providing a novel link of chromatin remodeling to hormonal and stress responses.

PMID:
19825649
DOI:
10.1093/mp/ssp026
[Indexed for MEDLINE]
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