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Biophys J. 2008 Jul;95(1):1-9. doi: 10.1529/biophysj.108.131789. Epub 2008 Apr 25.

Determination of protein structures--a series of fortunate events.

Author information

1
Department of Molecular Physiology and Biological Physics, University of Virginia, Charlottesville, Virginia, USA.

Abstract

Determination of a macromolecular structure using x-ray diffraction is a multistep process that involves a plethora of techniques involving molecular biology, bioinformatics, and physical sciences. Counterintuitively, the success of any or all individual steps does not guarantee the success of the overall process. This review examines the difficulties presented by each step on the path from a gene to the final publication, together with certain lucky (or unlucky) circumstances that can affect the velocity along that path.

PMID:
18441029
PMCID:
PMC2426657
DOI:
10.1529/biophysj.108.131789
[Indexed for MEDLINE]
Free PMC Article

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