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Nat Methods. 2007 Dec;4(12):1019-21. Epub 2007 Nov 4.

In situ proteolysis for protein crystallization and structure determination.

Author information

1
Structural Genomics Consortium, University of Toronto, 100 College Street, Toronto, Ontario M5G 1L5, Canada.

Abstract

We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.

PMID:
17982461
PMCID:
PMC3366506
DOI:
10.1038/nmeth1118
[Indexed for MEDLINE]
Free PMC Article

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