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Endocr J. 2005 Dec;52(6):769-73.

Mechanism of adrenomedullin-induced prolactin release from human prolactin-releasing adenoma cells.

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1
Department of Nephrology and Endocrinology, University of Tokyo Faculty of Medicine, Tokyo.

Abstract

The mechanism of adrenomedullin-induced prolactin release was investigated in prolactin-secreting human pituitary adenoma cells by intracellular calcium measurement and static incubation study. Adrenomedullin stimulated prolactin release in a concentration-dependent manner. The stimulation was dependent on extracellular sodium and voltage-gated calcium channels. PKA inhibitor attenuated adrenomedullin-induced prolactin release. The mechanism of adrenomedullin action was studied by fura 2-based intracellular calcium measurement. Adrenomedullin increased intracellular calcium concentration in these cells. The increase was dependent on extracellular sodium and voltage-gated calcium channels. PKA inhibitor attenuated the calcium response. These data indicate that adrenomedullin stimulates prolactin release by modulating calcium influx through voltage-gated calcium channels dependently on extracellular sodium. Mechanisms involving sodium-influx mediated depolarization may play a role in the stimulatory action.

PMID:
16410671
DOI:
10.1507/endocrj.52.769
[Indexed for MEDLINE]
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