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Fertil Steril. 2003 Mar;79 Suppl 1:763-9.

Cytokine regulation by peroxisome proliferator-activated receptor gamma in human endometrial cells.

Author information

1
Division of Research, Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, Georgia 30322, USA.

Abstract

OBJECTIVE:

To determine whether peroxisome proliferator-activated receptor (PPAR)-gamma ligands can affect the expression of interleukin-6 (IL-6) and cytokines related to the pathogenesis of endometriosis.

DESIGN:

In vitro study to determine whether PPARs are expressed in human endometrial cells and determine the effects of various PPAR-gamma ligands on IL-6 and other cytokine expression in these cells.

SETTING:

Academic medical center.

PATIENT(S):

Women presenting for infertility workup.

INTERVENTION(S):

Endometrial cell cultures were treated with PPAR-gamma ligands.

MAIN OUTCOME MEASURE(S):

Interleukin-6, IL-8, colony stimulating factor-1 (CSF-1) and macrophage chemotactic factor (MCP-1) protein secretion, messenger RNA expression of IL-6, PPAR-alpha, -beta, and -gamma.

RESULT(S):

Using a human endometrial cell line (EM42), as well as primary stromal and epithelial endometrial cells, we show the presence of PPAR-alpha, -beta, and -gamma by reverse transcription-polymerase chain reaction (RT-PCR) in these cells. PPAR-gamma ligands stimulated IL-6 secretion and induced enhancement of IL-6 mRNA levels. These ligands also stimulated the secretion of IL-8 and CSF-1.

CONCLUSION(S):

PPAR-gamma may play a role in the pathogenesis of endometriosis related to the production of IL-6 and some other cytokines.

PMID:
12620489
DOI:
10.1016/s0015-0282(02)04835-5
[Indexed for MEDLINE]

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