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Biochem Biophys Res Commun. 2015 Mar 27;459(1):54-9. doi: 10.1016/j.bbrc.2015.02.062. Epub 2015 Feb 21.

Cardiomyocyte lipotoxicity is mediated by Il-6 and causes down-regulation of PPARs.

Author information

1
Montreal Heart Institute, Université de Montréal, Canada; Department of Medicine, Université de Montréal, Canada.
2
Montreal Heart Institute, Université de Montréal, Canada.
3
Montreal Heart Institute, Université de Montréal, Canada; Department of Surgery, Université de Montréal, Canada. Electronic address: nicolas.bousette@umontreal.ca.

Abstract

Here we sought to evaluate the effect of palmitate on cytokine and PPAR activity/expression. We investigated the effect of BSA conjugated palmitate and oleate on PPAR activity, PPAR-α and δ expression, as well as the expression of cytokines and key factors responsible for β-oxidation by qRT-PCR and western blotting in primary rat neonatal cardiomyocytes (NCMs). Furthermore we evaluated the effect of anti-inflammatory actions of AICAR and PPAR agonists on cytokine expression and cell death in palmitate treated NCMs. We found that palmitate caused down regulation of PPARs and increased cytokine expression and cell death, all of which was significantly attenuated by the co-administration of either AICAR or PPAR agonists. This work supports the pro-inflammatory actions of intracellular lipid and provides further insight into the pathological mechanism of cardiac lipotoxicity as occurs in diabetic hearts.

KEYWORDS:

Cardiomyocytes; Cytokines; Fatty acids; Lipotoxicity; PPARs

PMID:
25712520
DOI:
10.1016/j.bbrc.2015.02.062
[Indexed for MEDLINE]

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