Interleukin 10 receptor alpha subunit (IL-10RA) gene polymorphism and IL-10 serum levels in Egyptian atopic patients

J Investig Allergol Clin Immunol. 2010;20(1):20-6.

Abstract

Objectives: To assess the value of serum interleukin (IL) 10 levels as an immunological marker in atopy and to determine the role of an IL-10RA gene single nucleotide polymorphism (SNP) (serine 138-to-glycine exchange [S138G]) in the pathogenesis of atopic diseases.

Methods: Seventy-five patients with atopic disorders were compared with 25 age-matched healthy volunteers. Serum total immunoglobulin (Ig) E and IL-10 levels were measured by enzyme-linked immunosorbent assay and the IL-10RA gene S138G variant was screened by multiplex allele-specific polymerase chain reaction.

Results: There was a significant association between G allele frequencies of the S138G variant (62%, 60% and 68% for atopic asthma, atopic dermatitis, and allergic rhinitis, respectively) in atopic patients compared to in controls. There were significant differences in mean IgE levels but not mean serum IL-10 levels between the allelic variants in atopy groups.

Conclusion: The IL-10RA gene SNP S138G may contribute to susceptibility to atopic diseases but serum IL-10 level is not a sensitive indicator in atopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Egypt
  • Gene Frequency
  • Genetic Predisposition to Disease
  • Genotype
  • Humans
  • Hypersensitivity / genetics*
  • Hypersensitivity / immunology
  • Immunoglobulin E / blood
  • Interleukin-10 / blood*
  • Polymorphism, Single Nucleotide*
  • Protein Subunits
  • Receptors, Interleukin-10 / genetics*

Substances

  • Protein Subunits
  • Receptors, Interleukin-10
  • Interleukin-10
  • Immunoglobulin E