[Evaluation of immunohistochemical markers for differential diagnosis of hepatocellular carcinoma from intrahepatic cholangiocarcinoma]

Zhonghua Zhong Liu Za Zhi. 2008 Sep;30(9):702-5.
[Article in Chinese]

Abstract

Objective: To evaluate the significance of a panel of immunohistochemical markers for distinguishing hepatocellular carcinoma (HCC) from intrahepatic cholangiocarcinoma (ICC).

Methods: Ten markers including hepatocyte paraffin 1 (Hep Par 1), polyclonal carcinoembryonic antigen (pCEA), CD34, CD10, CD105, multidrug resistance-associated protein-3 (MRP-3), cyclooxygenase-2 (COX-2), mucinous glycoprotein-1 (MUC-1), aquaporin-1 (AQP-1) and CK19 were immunohistochemically stained in the samples from 90 surgically resected HCC and 80 ICC, respectively,and the positive rate of their expression were compared statistically.

Results: The positive expression rates of Hep Par 1, pCEA, CD34, CD10, CD105, MRP-3, COX-2 were 85.6%, 82.2%, 87.8%, 18.9%, 8.9%, 11.1% and 48.9%, respectively, in HCC. While the positive expression rates of MUC-1, AQP-1 and CK19 were 73.8%, 65% and 92.5%, respectively, in ICC.

Conclusion: Based on our results, Hep Par 1 and CD34 can be used as the first line markers, and pCEA and COX-2 as the second line makers, for differential diagnosis of hepatocellular carcinoma from intrahepatic cholangiocarcinoma. While MUC-1 and CK19 can be used as the first line markers and AQP-1 as the second one for the differential diagnosis of intrahepatic cholangiocarcinoma from hepatocellular carcinoma.

Publication types

  • Evaluation Study

MeSH terms

  • Bile Duct Neoplasms / chemistry
  • Bile Duct Neoplasms / diagnosis*
  • Bile Ducts, Intrahepatic / chemistry
  • Biomarkers, Tumor / analysis*
  • Carcinoma, Hepatocellular / chemistry
  • Carcinoma, Hepatocellular / diagnosis*
  • Cholangiocarcinoma / chemistry
  • Cholangiocarcinoma / diagnosis*
  • Diagnosis, Differential
  • Female
  • Hepatocytes / chemistry
  • Hepatocytes / pathology
  • Humans
  • Immunohistochemistry
  • Liver Neoplasms / chemistry
  • Liver Neoplasms / diagnosis*
  • Male
  • Middle Aged

Substances

  • Biomarkers, Tumor