Abstract
The methylation status of seven cancer-related genes was investigated in a series of 58 colorectal cancers, 18 of which showed the microsatellite instability (MSI+) phenotype. Methylation of the hMLH1, p16 and MDR1 genes was found in 23, 29 and 28% of tumors, respectively. None of the tumors showed methylation of the TS, ATM, PARP or p21 genes. Methylation of the hMLH1, p16 and MDR1 genes was more frequent and more concordant in MSI+ compared to MSI- tumors (P<0.001) and was also strongly associated with poor histological differentiation (P<0.001). There were trends for associations between methylation at one or more of these loci and proximal tumor location, advanced Dukes' stage and the presence of wild-type p53 (P=0.06 for each).
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Adult
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Aged
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Ataxia Telangiectasia Mutated Proteins
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Carrier Proteins
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Cell Cycle Proteins
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Colorectal Neoplasms / genetics*
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Colorectal Neoplasms / metabolism
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Colorectal Neoplasms / pathology
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CpG Islands / genetics
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Cyclin-Dependent Kinase Inhibitor p21
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Cyclins / genetics
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DNA Methylation*
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DNA Primers
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DNA-Binding Proteins
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Female
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Genes, MDR*
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Genes, p16*
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Humans
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Male
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Microsatellite Repeats / genetics
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Middle Aged
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MutL Protein Homolog 1
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Neoplasm Proteins / genetics*
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Nuclear Proteins
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Poly(ADP-ribose) Polymerases / genetics
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Polymorphism, Single-Stranded Conformational
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Protein Serine-Threonine Kinases / genetics
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Thymidylate Synthase / genetics
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Tumor Suppressor Proteins
Substances
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Adaptor Proteins, Signal Transducing
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CDKN1A protein, human
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Carrier Proteins
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Cell Cycle Proteins
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Cyclin-Dependent Kinase Inhibitor p21
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Cyclins
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DNA Primers
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DNA-Binding Proteins
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MLH1 protein, human
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Neoplasm Proteins
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Nuclear Proteins
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Tumor Suppressor Proteins
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Thymidylate Synthase
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Poly(ADP-ribose) Polymerases
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ATM protein, human
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Ataxia Telangiectasia Mutated Proteins
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Protein Serine-Threonine Kinases
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MutL Protein Homolog 1