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Protein Sci. 2015 Oct;24(10):1600-8. doi: 10.1002/pro.2743. Epub 2015 Jul 30.

Cofactor-induced reversible folding of Flavodoxin-4 from Lactobacillus acidophilus.

Author information

1
Joint Center for Structural Genomics, La Jolla, California, 92037.
2
Department of Integrative Structural and Computational Biology, the Scripps Research Institute, La Jolla, California, 92037.
3
SLAC National Accelerator Laboratory, Stanford Synchrotron Radiation Lightsource, California, 94025.
4
Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, California, 92121.
5
Program on Bioinformatics and Systems Biology, Sanford-Burnham Medical Research Institute, La Jolla, California, 92037.
6
Center for Research in Biological Systems, University of California, San Diego, La Jolla, California, 92093.
7
Skaggs Institute for Chemical Biology, the Scripps Research Institute, La Jolla, California, 92037.

Abstract

Flavodoxins in combination with the flavin mononucleotide (FMN) cofactor play important roles for electron transport in prokaryotes. Here, novel insights into the FMN-binding mechanism to flavodoxins-4 were obtained from the NMR structures of the apo-protein from Lactobacillus acidophilus (YP_193882.1) and comparison of its complex with FMN. Extensive reversible conformational changes were observed upon FMN binding and release. The NMR structure of the FMN complex is in agreement with the crystal structure (PDB ID: 3EDO) and exhibits the characteristic flavodoxin fold, with a central five-stranded parallel β-sheet and five α-helices forming an α/β-sandwich architecture. The structure differs from other flavoproteins in that helix α2 is oriented perpendicular to the β-sheet and covers the FMN-binding site. This helix reversibly unfolds upon removal of the FMN ligand, which represents a unique structural rearrangement among flavodoxins.

KEYWORDS:

cofactor binding; flavin mononucleotide; protein folding; protein-ligand interaction

PMID:
26177955
PMCID:
PMC4594659
DOI:
10.1002/pro.2743
[Indexed for MEDLINE]
Free PMC Article

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