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Anal Chem. 2015 Aug 4;87(15):7540-4. doi: 10.1021/acs.analchem.5b01410. Epub 2015 Jul 22.

Isotope-Coded Labeling for Accelerated Protein Interaction Profiling Using MS.

Author information

1
†Genomics Institute of the Novartis Research Foundation, San Diego, California 92121, United States.
2
‡Joint Center for Structural Genomics, La Jolla, California 92037, United States, http://www.jcsg.org.

Abstract

Protein interaction surface mapping using MS is widely applied but comparatively resource-intensive. Here, a workflow adaptation for use of isotope-coded tandem mass tags for the purpose is reported. The key benefit of improved throughput derived from sample acquisition multiplexing and automated analysis is shown to be maintained in the new application. Mapping of the epitopes of two monoclonal antibodies on their respective targets serves to illustrate the novel approach. We conclude that the approach enables mapping of interactions by MS at significantly larger scales than hereto possible.

PMID:
26151661
PMCID:
PMC4535432
DOI:
10.1021/acs.analchem.5b01410
[Indexed for MEDLINE]
Free PMC Article

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