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PLoS One. 2015 Mar 31;10(3):e0122512. doi: 10.1371/journal.pone.0122512. eCollection 2015.

Crystal structure of a two-subunit TrkA octameric gating ring assembly.

Author information

1
The Joint Center for Structural Genomics, and Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, United States of America.
2
The Joint Center for Structural Genomics, and Stanford Synchrotron Radiation Lightsource, SLAC National Accelerator Laboratory, Menlo Park, California, United States of America.
3
Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, California, United States of America.
4
The Joint Center for Structural Genomics, and Department of Integrative Structural and Computational Biology, The Scripps Research Institute, La Jolla, California, United States of America; Protein Sciences Department, Genomics Institute of the Novartis Research Foundation, San Diego, California, United States of America.

Abstract

The TM1088 locus of T. maritima codes for two proteins designated TM1088A and TM1088B, which combine to form the cytosolic portion of a putative Trk K+ transporter. We report the crystal structure of this assembly to a resolution of 3.45 Å. The high resolution crystal structures of the components of the assembly, TM1088A and TM1088B, were also determined independently to 1.50 Å and 1.55 Å, respectively. The TM1088 proteins are structurally homologous to each other and to other K+ transporter proteins, such as TrkA. These proteins form a cytosolic gating ring assembly that controls the flow of K+ ions across the membrane. TM1088 represents the first structure of a two-subunit Trk assembly. Despite the atypical genetics and chain organization of the TM1088 assembly, it shares significant structural homology and an overall quaternary organization with other single-subunit K+ gating ring assemblies. This structure provides the first structural insights into what may be an evolutionary ancestor of more modern single-subunit K+ gating ring assemblies.

PMID:
25826626
PMCID:
PMC4380455
DOI:
10.1371/journal.pone.0122512
[Indexed for MEDLINE]
Free PMC Article

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