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Protein Sci. 2011 Jul;20(7):1137-44. doi: 10.1002/pro.641. Epub 2011 Jun 14.

NMR structure of the Bordetella bronchiseptica protein NP_888769.1 establishes a new phage-related protein family PF13554.

Author information

1
Joint Center for Structural Genomics (http://www.jcsg.org), The Scripps Research Institute, La Jolla, California 92037, USA.

Abstract

The solution structure of the hypothetical phage-related protein NP_888769.1 from the Gram-negative bacterium Bordetella bronchoseptica contains a well-structured core comprising a five-stranded, antiparallel β-sheet packed on one side against two α-helices and a short β-hairpin with three flexibly disordered loops extending from the central β-sheet. A homology search with the software DALI identified two Protein Data Bank deposits with Z-scores > 8, where both of these proteins have less than 8% sequence identity relative to NP_888769.1, and one has been functionally annotated as a lambda phage tail terminator protein. A sequence-homology analysis then confirmed that NP_888769.1 represents the first three-dimensional structural representative of a new protein family that was previously predicted by the Joint Center for Structural Genomics, which includes so far about 20 prophage proteins encoded in bacterial genomes.

PMID:
21520320
PMCID:
PMC3149187
DOI:
10.1002/pro.641
[Indexed for MEDLINE]
Free PMC Article

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