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Biochim Biophys Acta. 1987 Dec 11;905(2):499-502.

A simple and rapid method for the purification of the mitochondrial porin from mammalian tissues.

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Department of Pharmaco-Biology, University of Bari, Italy.


A new, simple and rapid procedure for the purification of high amounts of mitochondrial porins from different tissues of mammalia is described. The method consists in a single step hydroxyapatite/celite chromatography of Triton X-100 solubilized mitochondrial membranes. For optimal purification several factors are critical such as the absence of salts, a low protein/detergent ratio and an exact hydroxyapatite/celite ratio of 2:1.

[Indexed for MEDLINE]

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