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Bioprocess Biosyst Eng. 2007 May;30(3):157-64. Epub 2007 Feb 4.

Effect of bioactive peptides isolated from yeastolate, lactalbumin and NZCase in the insect cell growth.

Author information

1
Laboratório de Imunologia Viral, Instituto Butantan, CEP, São Paulo, SP, Brazil. zucatelli@uol.com.br

Abstract

In this study, we have described the biological activity of various hydrolysates and its effect on cell growth, growth rate and doubling time. A potent cell culture enhancer factor was observed in the yeastolate hydrolysates, mainly in the protein fractions with low molecular weight. In this case, a growth enhancer of 60.66% was obtained. Despite a lower efficiency of crude lactalbumin hydrolysates (14%), when lactalbumin and yeastolate were added together to the culture, the cell yields were of 102%, showing a synergic effect. Nevertheless, sub fraction from LMW, of lactalbumin, obtained by Sephadex G-10 gel filtration chromatography showed a higher positive effect (23.3%) than low molecular weight fraction of lactalbumin without this chromatography step (11.3%). It is suggested that low molecular weight lactalbumin could have some inhibitory protein. On the other hand, NZCase low molecular weight showed a positive effect of 29.33%, while its sub fractions showed a negative effect of 5.5%. With these data we can suggest that these hydrolysates could be an important element to design new media, serum free, being helpful in protein recombinant production.

PMID:
17278046
DOI:
10.1007/s00449-006-0099-3
[Indexed for MEDLINE]

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