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J Biol Chem. 2004 Jan 30;279(5):3680-4. Epub 2003 Dec 11.

Annexin II is required for apical transport in polarized epithelial cells.

Author information

1
Department of Physiological Chemistry, School of Veterinary Medicine Hannover, Germany. jacob@staff.uni-marburg.de

Abstract

The sorting of apical proteins comprises an initial recognition step in the trans Golgi network and a final partitioning of the apical pool of proteins into at least two different types of vesicular carriers. One criteria of these carriers is the association or non-association of the protein content with lipid rafts. We have previously characterized a population containing the raft-associated sucrase-isomaltase-carrying vesicles (SAVs) and another one, the non-raft-associated lactase-phlorizin hydrolase-carrrying vesicles (LAVs) that are targeted separately to the apical membrane. Here, we demonstrate biochemically and by employing confocal laser microscopy that the annexin II-S100A10 complex is a component of SAVs and is absent from LAVs. The unequivocal role of annexin II in the apical targeting of SI is clearly demonstrated when down-regulation of this protein by annexin II-specific small interfering RNA drastically decreases the apical delivery of SI in the epithelial cell line Madin-Darby canine kidney. The annexin II-S100A10 complex plays therefore a crucial role in routing SAVs to the apical membrane of epithelial cells.

PMID:
14670963
DOI:
10.1074/jbc.C300503200
[Indexed for MEDLINE]
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